Mutagenetix > Incidental Mutation

Incidental Mutation 'R6089:Zscan26'

484678

Institutional Source

Beutler Lab

Gene Symbol

Zscan26

Ensembl Gene

ENSMUSG00000022228

Gene Name

zinc finger and SCAN domain containing 26

Synonyms

Zfp187

MMRRC Submission

044246-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R6089 (G1)

Quality Score

192.009

Status

Not validated

Chromosome

Chromosomal Location

21442180-21453730 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 21448329 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 77 (S77R)

Ref Sequence

ENSEMBL: ENSMUSP00000106111 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032820] [ENSMUST00000110485]

AlphaFold

Q5RJ54

Predicted Effect

probably benign
Transcript: ENSMUST00000032820

SMART Domains

Protein: ENSMUSP00000032820
Gene: ENSMUSG00000022228

Domain	Start	End	E-Value	Type
ZnF_C2H2	144	166	1.58e-3	SMART
ZnF_C2H2	172	194	6.99e-5	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	228	250	1.45e-2	SMART
ZnF_C2H2	256	278	2.53e-2	SMART
ZnF_C2H2	284	306	5.14e-3	SMART
ZnF_C2H2	312	334	2.95e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000110485
AA Change: S77R

PolyPhen 2 Score 0.969 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000106111
Gene: ENSMUSG00000022228
AA Change: S77R

Domain	Start	End	E-Value	Type
SCAN	38	151	1.58e-48	SMART
ZnF_C2H2	270	292	1.58e-3	SMART
ZnF_C2H2	298	320	6.99e-5	SMART
ZnF_C2H2	326	348	1.67e-2	SMART
ZnF_C2H2	354	376	1.45e-2	SMART
ZnF_C2H2	382	404	2.53e-2	SMART
ZnF_C2H2	410	432	5.14e-3	SMART
ZnF_C2H2	438	460	2.95e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122641

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122926

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.8%
20x: 93.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9030624J02Rik	T	G	7: 118,746,435	S59A	possibly damaging	Het
A830010M20Rik	C	T	5: 107,507,029	A386V	probably damaging	Het
Actr3	A	G	1: 125,407,395	V95A	probably damaging	Het
Ankrd40	A	T	11: 94,333,925	K53N	probably damaging	Het
Ash1l	C	T	3: 89,053,143	Q2338*	probably null	Het
Astn2	A	G	4: 65,794,573	F610S	probably damaging	Het
Baz2a	TCTCCTC	TCTC	10: 128,114,642		probably benign	Het
Calm4	A	T	13: 3,837,874		probably benign	Het
Card10	T	C	15: 78,802,414	T68A	probably benign	Het
Cars2	A	G	8: 11,530,301	M245T	probably damaging	Het
Cat	C	A	2: 103,472,951	L160F	probably null	Het
Cers5	G	T	15: 99,741,002	T144K	probably benign	Het
Cyp3a13	T	A	5: 137,909,953	L211F	probably benign	Het
Defb4	A	T	8: 19,201,305	R63*	probably null	Het
Dnah14	G	T	1: 181,750,154	W3012L	probably damaging	Het
Dusp18	A	G	11: 3,897,555	T182A	probably benign	Het
Dzip1l	C	A	9: 99,642,684	T286K	possibly damaging	Het
Eif2ak3	A	T	6: 70,896,934	T944S	possibly damaging	Het
Fbn1	A	T	2: 125,321,225	I2119N	possibly damaging	Het
Gabbr2	A	G	4: 46,846,448	S154P	probably damaging	Het
Gm15440	A	T	13: 51,691,401	H107Q	unknown	Het
Grm2	G	A	9: 106,653,891	P133L	probably damaging	Het
Gsdme	A	G	6: 50,251,305	V61A	probably damaging	Het
Gtpbp1	A	G	15: 79,706,997	T23A	probably benign	Het
Helz	A	T	11: 107,595,137		probably null	Het
Herc1	A	C	9: 66,445,532	H2100P	probably damaging	Het
Hjurp	GT	GTT	1: 88,266,524		probably null	Het
Ifngr1	T	C	10: 19,606,300	S213P	probably benign	Het
Itgb7	T	C	15: 102,217,286	D636G	probably benign	Het
Mre11a	G	A	9: 14,819,464	D491N	probably benign	Het
Muc16	A	G	9: 18,643,252	L3915P	unknown	Het
Myh1	T	A	11: 67,202,167		probably null	Het
Myh1	T	G	11: 67,220,787		probably null	Het
Myh7b	C	T	2: 155,622,489	T608M	probably damaging	Het
Myof	T	C	19: 37,967,060	K23E	probably benign	Het
Nacad	A	G	11: 6,601,331	V620A	probably benign	Het
Pbrm1	T	C	14: 31,087,585	Y1121H	probably damaging	Het
Pcdha9	T	C	18: 36,998,498	F207L	probably benign	Het
Pcdhb10	T	C	18: 37,413,626	V585A	possibly damaging	Het
Pigg	A	T	5: 108,341,922	M798L	probably benign	Het
Poc5	G	T	13: 96,396,671	M102I	probably damaging	Het
Ppp2r3a	T	A	9: 101,211,636	Q496L	probably benign	Het
Ptprf	A	G	4: 118,211,084	Y1752H	probably damaging	Het
Ptpru	G	C	4: 131,772,630	S1244R	possibly damaging	Het
Rnf213	A	G	11: 119,416,559	K682E	probably benign	Het
Rtp3	T	C	9: 110,986,973	D170G	probably benign	Het
Sall4	T	C	2: 168,755,486	D478G	possibly damaging	Het
Scai	A	T	2: 39,083,554	Y58*	probably null	Het
Simc1	G	A	13: 54,528,490	V1007M	probably benign	Het
Tcf20	G	T	15: 82,853,208	D1347E	probably benign	Het
Tdo2	T	C	3: 81,962,728	Y279C	probably damaging	Het
Trdn	C	T	10: 33,464,575	P602S	probably benign	Het
Txndc5	A	T	13: 38,523,416	M1K	probably null	Het
Ubr2	A	C	17: 46,982,292	M356R	possibly damaging	Het
Uqcrc2	A	T	7: 120,654,009	D332V	probably damaging	Het
Usp48	T	C	4: 137,605,818	F108L	probably damaging	Het
Vill	A	G	9: 119,057,799	N4S	probably benign	Het
Vmn1r11	T	C	6: 57,137,660	V103A	possibly damaging	Het
Vmn2r98	A	G	17: 19,066,074	D278G	probably benign	Het
Wfdc15a	A	T	2: 164,199,666	L79*	probably null	Het
Xxylt1	C	T	16: 31,080,981	V119M	probably damaging	Het
Zfp26	G	T	9: 20,437,693	A525D	probably damaging	Het
Zgrf1	G	A	3: 127,595,993	G396D	probably damaging	Het
Zkscan16	A	G	4: 58,948,889	D148G	possibly damaging	Het

Other mutations in Zscan26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Zscan26	APN	13	21445101	missense	probably damaging	0.99
IGL00720:Zscan26	APN	13	21445199	missense	probably benign	0.00
R0558:Zscan26	UTSW	13	21445055	missense	probably benign	0.37
R1236:Zscan26	UTSW	13	21445770	missense	probably benign
R1912:Zscan26	UTSW	13	21445140	missense	possibly damaging	0.84
R4692:Zscan26	UTSW	13	21445257	missense	probably damaging	1.00
R5818:Zscan26	UTSW	13	21445761	missense	probably benign	0.00
R8029:Zscan26	UTSW	13	21445350	missense	probably damaging	1.00
R9448:Zscan26	UTSW	13	21448261	missense	probably benign
Z1088:Zscan26	UTSW	13	21445463	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TACTTTCCTGCAAATGGGCAC -3'
(R):5'- CCAAGTCTGTGCTTTAAGCCAC -3'

Sequencing Primer
(F):5'- GGCACCTGCACCCCTTAC -3'
(R):5'- CCACTGTTAAAACTACTGTGCTTGGG -3'

Posted On

2017-08-16