Mutagenetix > Incidental Mutation

Incidental Mutation 'R6111:Zfp933'

484834

Institutional Source

Beutler Lab

Gene Symbol

Zfp933

Ensembl Gene

ENSMUSG00000059423

Gene Name

zinc finger protein 933

Synonyms

2810408P10Rik

MMRRC Submission

044260-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R6111 (G1)

Quality Score

215.009

Status

Validated

Chromosome

Chromosomal Location

147907443-147932823 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 147913217 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 14 (T14A)

Ref Sequence

ENSEMBL: ENSMUSP00000101343 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105718] [ENSMUST00000135798]

AlphaFold

Q6PEE4

Predicted Effect

probably damaging
Transcript: ENSMUST00000105718
AA Change: T14A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101343
Gene: ENSMUSG00000059423
AA Change: T14A

Domain	Start	End	E-Value	Type
KRAB	4	66	9.49e-16	SMART
ZnF_C2H2	131	153	3.21e-4	SMART
ZnF_C2H2	159	181	5.21e-4	SMART
ZnF_C2H2	187	209	2.12e-4	SMART
ZnF_C2H2	215	237	7.26e-3	SMART
ZnF_C2H2	243	265	6.88e-4	SMART
ZnF_C2H2	271	293	1.13e-4	SMART
ZnF_C2H2	299	321	3.95e-4	SMART
ZnF_C2H2	327	349	1.56e-2	SMART
ZnF_C2H2	355	377	1.79e-2	SMART
ZnF_C2H2	383	405	4.24e-4	SMART
ZnF_C2H2	411	433	1.22e-4	SMART
ZnF_C2H2	439	461	4.79e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135798

SMART Domains

Protein: ENSMUSP00000118300
Gene: ENSMUSG00000059423

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	8e-18	BLAST
PDB:2I13\|B	32	98	1e-12	PDB
SCOP:d1fgja_	33	98	5e-13	SMART
Blast:PHD	44	98	6e-11	BLAST

Meta Mutation Damage Score

0.5205

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.2%

Validation Efficiency

98% (57/58)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	A	G	13: 77,476,021 (GRCm39)	E1110G	possibly damaging	Het
Abcd4	G	A	12: 84,661,888 (GRCm39)	T79I	probably damaging	Het
Acd	A	G	8: 106,424,919 (GRCm39)	M407T	probably benign	Het
Adcy2	T	A	13: 68,877,360 (GRCm39)	H460L	probably damaging	Het
Atad2	G	A	15: 57,971,487 (GRCm39)	H752Y	probably benign	Het
Bmal2	T	C	6: 146,722,097 (GRCm39)	F223L	probably benign	Het
Camsap2	A	G	1: 136,209,036 (GRCm39)	S819P	probably benign	Het
Col24a1	C	A	3: 145,019,815 (GRCm39)	T62K	probably damaging	Het
Cpne6	G	A	14: 55,752,091 (GRCm39)	V283M	probably benign	Het
D630003M21Rik	A	G	2: 158,055,368 (GRCm39)	S590P	probably damaging	Het
Daam1	T	A	12: 71,989,038 (GRCm39)	M146K	unknown	Het
Dclk2	A	G	3: 86,712,968 (GRCm39)	Y495H	probably benign	Het
Ddx4	A	T	13: 112,757,766 (GRCm39)	C330*	probably null	Het
Dlec1	T	C	9: 118,931,692 (GRCm39)	L37P	possibly damaging	Het
Dock2	G	A	11: 34,599,614 (GRCm39)	P322S	probably damaging	Het
Espl1	A	G	15: 102,208,323 (GRCm39)	E443G	probably damaging	Het
Eya4	T	A	10: 23,015,953 (GRCm39)	D338V	possibly damaging	Het
Fcmr	A	G	1: 130,805,566 (GRCm39)	I267V	probably damaging	Het
Gfra3	T	A	18: 34,823,927 (GRCm39)	H349L	probably damaging	Het
Gm25747	A	G	12: 113,392,703 (GRCm39)		probably benign	Het
Gria4	G	A	9: 4,502,430 (GRCm39)	R368C	probably damaging	Het
H2-Q5	A	T	17: 35,613,885 (GRCm39)	I145F	possibly damaging	Het
Hace1	A	T	10: 45,465,606 (GRCm39)	K54I	possibly damaging	Het
Ift122	T	A	6: 115,852,247 (GRCm39)	I79N	probably damaging	Het
Ino80b	A	G	6: 83,101,347 (GRCm39)	V121A	probably damaging	Het
Kcnq1	A	G	7: 142,661,474 (GRCm39)	T63A	probably benign	Het
Map4k4	C	A	1: 40,050,822 (GRCm39)	Q762K	probably benign	Het
Mios	G	A	6: 8,214,836 (GRCm39)	A11T	probably benign	Het
Nfatc1	A	G	18: 80,741,125 (GRCm39)	S278P	probably damaging	Het
Notch2	T	C	3: 98,053,609 (GRCm39)	S2091P	probably benign	Het
Nudt19	T	A	7: 35,254,952 (GRCm39)	D93V	probably benign	Het
Or8b12b	T	C	9: 37,684,228 (GRCm39)	I91T	probably damaging	Het
Osbpl2	A	G	2: 179,791,994 (GRCm39)	T233A	probably benign	Het
P3h1	C	T	4: 119,098,329 (GRCm39)	R369*	probably null	Het
Pcdhb3	A	T	18: 37,435,242 (GRCm39)	I403L	probably benign	Het
Pigo	T	C	4: 43,019,724 (GRCm39)	D935G	probably benign	Het
Plpp1	A	G	13: 113,003,451 (GRCm39)	H224R	probably damaging	Het
Rai1	T	A	11: 60,078,732 (GRCm39)	M932K	probably damaging	Het
Rexo2	A	T	9: 48,384,412 (GRCm39)	F122L	probably damaging	Het
Rsf1	GCG	GCGACGGCGACG	7: 97,229,114 (GRCm39)		probably benign	Het
Sdc3	A	G	4: 130,546,153 (GRCm39)	T77A	unknown	Het
Skint5	T	C	4: 113,562,845 (GRCm39)	T786A	unknown	Het
Smok3c	C	A	5: 138,063,365 (GRCm39)	P284Q	probably damaging	Het
Spg11	A	G	2: 121,923,963 (GRCm39)	V786A	probably damaging	Het
Tmem273	T	C	14: 32,528,755 (GRCm39)	I40T	possibly damaging	Het
Tnfrsf10b	T	A	14: 70,020,007 (GRCm39)	C380S	possibly damaging	Het
Tsen54	T	C	11: 115,710,956 (GRCm39)	V176A	possibly damaging	Het
Ttll4	A	T	1: 74,736,698 (GRCm39)	K1141M	possibly damaging	Het
Ttpa	A	T	4: 20,014,772 (GRCm39)	I116F	probably damaging	Het
Tubgcp6	T	C	15: 88,985,123 (GRCm39)	D1655G	possibly damaging	Het
Usp38	A	G	8: 81,740,551 (GRCm39)	V172A	probably damaging	Het
Vmn2r73	A	G	7: 85,520,997 (GRCm39)	S324P	probably benign	Het
Wdr72	T	C	9: 74,117,607 (GRCm39)	M773T	probably benign	Het
Xirp2	T	A	2: 67,342,161 (GRCm39)	H1467Q	possibly damaging	Het
Zdhhc8	G	T	16: 18,042,762 (GRCm39)	S479R	probably damaging	Het
Zfp423	A	G	8: 88,509,315 (GRCm39)	V322A	probably damaging	Het
Zfp87	T	G	13: 74,520,504 (GRCm39)	E191D	probably benign	Het

Other mutations in Zfp933

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00576:Zfp933	APN	4	147,910,778 (GRCm39)	missense	probably damaging	1.00
IGL03377:Zfp933	APN	4	147,913,168 (GRCm39)	missense	possibly damaging	0.65
F5770:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
FR4340:Zfp933	UTSW	4	147,910,186 (GRCm39)	frame shift	probably null
FR4548:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
R0388:Zfp933	UTSW	4	147,910,899 (GRCm39)	missense	probably benign	0.35
R0523:Zfp933	UTSW	4	147,910,919 (GRCm39)	nonsense	probably null
R0539:Zfp933	UTSW	4	147,911,005 (GRCm39)	missense	probably benign	0.08
R1672:Zfp933	UTSW	4	147,910,476 (GRCm39)	missense	probably damaging	1.00
R4049:Zfp933	UTSW	4	147,910,969 (GRCm39)	missense	probably damaging	1.00
R4895:Zfp933	UTSW	4	147,910,892 (GRCm39)	nonsense	probably null
R5133:Zfp933	UTSW	4	147,911,321 (GRCm39)	missense	probably benign
R5786:Zfp933	UTSW	4	147,912,864 (GRCm39)	splice site	probably null
R5891:Zfp933	UTSW	4	147,911,231 (GRCm39)	missense	probably benign	0.03
R6382:Zfp933	UTSW	4	147,910,325 (GRCm39)	missense	probably benign	0.07
R6968:Zfp933	UTSW	4	147,910,654 (GRCm39)	missense	probably damaging	1.00
R7195:Zfp933	UTSW	4	147,910,636 (GRCm39)	missense	probably benign	0.16
R7555:Zfp933	UTSW	4	147,910,589 (GRCm39)	missense	probably damaging	1.00
R7902:Zfp933	UTSW	4	147,911,058 (GRCm39)	missense	probably damaging	0.96
R8319:Zfp933	UTSW	4	147,912,910 (GRCm39)	missense	possibly damaging	0.87
R8688:Zfp933	UTSW	4	147,911,249 (GRCm39)	missense	probably benign	0.14
R9019:Zfp933	UTSW	4	147,911,021 (GRCm39)	missense	probably damaging	1.00
RF024:Zfp933	UTSW	4	147,910,898 (GRCm39)	missense	probably damaging	1.00
RF028:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
RF035:Zfp933	UTSW	4	147,910,188 (GRCm39)	makesense	probably null
RF043:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
V7581:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7582:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7583:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- ACCTTCCACGTCTTCTAGAGC -3'
(R):5'- TATCCTACTCAAAGGTTGCAGAAAT -3'

Sequencing Primer
(F):5'- TGCTTCAGTGAGCCTTAGAAC -3'
(R):5'- CCTTCTAAAATCACTTGGAGATGAC -3'

Posted On

2017-08-16