Mutagenetix > Incidental Mutation

Incidental Mutation 'R6115:Scn9a'

485059

Institutional Source

Beutler Lab

Gene Symbol

Scn9a

Ensembl Gene

ENSMUSG00000075316

Gene Name

sodium channel, voltage-gated, type IX, alpha

Synonyms

PN1

MMRRC Submission

044264-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6115 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66480080-66634962 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 66563629 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 200 (Y200F)

Ref Sequence

ENSEMBL: ENSMUSP00000097641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100063] [ENSMUST00000100064] [ENSMUST00000112354] [ENSMUST00000164384] [ENSMUST00000169900]

AlphaFold

Q62205

Predicted Effect

possibly damaging
Transcript: ENSMUST00000100063
AA Change: Y200F

PolyPhen 2 Score 0.705 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000097641
Gene: ENSMUSG00000075316
AA Change: Y200F

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	403	9.5e-78	PFAM
coiled coil region	404	442	N/A	INTRINSIC
Pfam:DUF3451	465	685	1.3e-62	PFAM
Pfam:Ion_trans	768	957	9.9e-48	PFAM
Pfam:Na_trans_assoc	972	1191	2.9e-72	PFAM
low complexity region	1203	1214	N/A	INTRINSIC
Pfam:Ion_trans	1217	1445	2.8e-55	PFAM
PDB:1BYY\|A	1447	1499	9e-27	PDB
Pfam:Ion_trans	1538	1748	3.4e-52	PFAM
Pfam:PKD_channel	1599	1755	1.1e-7	PFAM
IQ	1877	1899	1.03e-3	SMART
low complexity region	1956	1972	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100064
AA Change: Y200F

PolyPhen 2 Score 0.325 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000097642
Gene: ENSMUSG00000075316
AA Change: Y200F

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	125	412	2.2e-84	PFAM
low complexity region	433	446	N/A	INTRINSIC
Pfam:Na_trans_cytopl	483	693	7.5e-76	PFAM
Pfam:Ion_trans	742	977	4.1e-57	PFAM
Pfam:Na_trans_assoc	981	1185	1.4e-58	PFAM
Pfam:Ion_trans	1189	1466	7e-67	PFAM
Pfam:Ion_trans	1512	1769	1e-55	PFAM
Pfam:PKD_channel	1605	1763	2.6e-7	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112354
AA Change: Y200F

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000107973
Gene: ENSMUSG00000075316
AA Change: Y200F

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.2e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141603

Predicted Effect

probably benign
Transcript: ENSMUST00000164384
AA Change: Y200F

PolyPhen 2 Score 0.325 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000126528
Gene: ENSMUSG00000075316
AA Change: Y200F

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.1e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	694	4.2e-66	PFAM
Pfam:Ion_trans	777	966	8.8e-48	PFAM
Pfam:Na_trans_assoc	981	1200	6e-72	PFAM
low complexity region	1212	1223	N/A	INTRINSIC
Pfam:Ion_trans	1226	1454	2.5e-55	PFAM
PDB:1BYY\|A	1456	1508	6e-29	PDB
Pfam:Ion_trans	1547	1757	3e-52	PFAM
Pfam:PKD_channel	1608	1764	8.1e-8	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000169900

SMART Domains

Protein: ENSMUSP00000131711
Gene: ENSMUSG00000075316

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	3.7e-78	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Meta Mutation Damage Score

0.1343

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 95.9%

Validation Efficiency

98% (51/52)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal/neonatal lethality. Mice homozygous for a knock-in allele exhibit increased susceptibility to electrically induced seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	C	G	5: 63,897,974 (GRCm38)	Q18E	probably damaging	Het
Acsf3	T	A	8: 122,790,672 (GRCm38)	H402Q	probably damaging	Het
Adam34	T	G	8: 43,652,061 (GRCm38)	Q182H	probably benign	Het
Alx1	G	A	10: 103,028,443 (GRCm38)	P55L	possibly damaging	Het
Arhgef38	A	T	3: 133,132,613 (GRCm38)		probably null	Het
Ccdc102a	T	C	8: 94,903,371 (GRCm38)	N514S	probably benign	Het
Corin	T	A	5: 72,360,729 (GRCm38)	T317S	probably damaging	Het
Ctnna2	A	G	6: 77,636,839 (GRCm38)	V256A	probably benign	Het
Dhx29	A	T	13: 112,952,801 (GRCm38)		probably null	Het
Dnah2	C	A	11: 69,446,649 (GRCm38)	D3209Y	probably damaging	Het
Dnhd1	A	G	7: 105,713,987 (GRCm38)	T3919A	probably benign	Het
F7	T	A	8: 13,033,958 (GRCm38)	N214K	probably benign	Het
Fam3b	T	G	16: 97,475,368 (GRCm38)	Q177H	possibly damaging	Het
Fign	T	C	2: 63,979,310 (GRCm38)	I539V	probably benign	Het
Hc	T	G	2: 35,013,038 (GRCm38)	D1067A	probably damaging	Het
Herc6	A	G	6: 57,583,206 (GRCm38)	D77G	probably benign	Het
Hmg20a	A	T	9: 56,489,832 (GRCm38)	E305D	possibly damaging	Het
Il16	G	A	7: 83,652,567 (GRCm38)	Q116*	probably null	Het
Kif13a	T	A	13: 46,801,313 (GRCm38)	I648F	probably damaging	Het
Lactb	G	T	9: 66,967,687 (GRCm38)	N374K	possibly damaging	Het
Lmx1b	T	C	2: 33,569,106 (GRCm38)	D145G	probably damaging	Het
Lrfn4	T	C	19: 4,613,909 (GRCm38)	D199G	probably damaging	Het
Lrrc49	A	T	9: 60,615,161 (GRCm38)	V307E	possibly damaging	Het
Magi1	A	C	6: 93,708,070 (GRCm38)	S776A	possibly damaging	Het
Mthfsl	T	A	9: 88,688,754 (GRCm38)	*147L	probably null	Het
Nsmce4a	G	T	7: 130,546,992 (GRCm38)	Q95K	probably benign	Het
Or1j15	A	T	2: 36,568,951 (GRCm38)	M118L	probably damaging	Het
Or4k37	A	G	2: 111,329,213 (GRCm38)	T265A	probably benign	Het
Or4k49	A	T	2: 111,664,642 (GRCm38)	K139*	probably null	Het
Or5b24	T	C	19: 12,935,584 (GRCm38)	V282A	possibly damaging	Het
Pcdha7	A	G	18: 36,974,735 (GRCm38)	E271G	probably damaging	Het
Pcdhga8	T	A	18: 37,727,543 (GRCm38)	F551I	possibly damaging	Het
Prpf31	T	C	7: 3,639,706 (GRCm38)		probably null	Het
Qrfpr	C	T	3: 36,182,593 (GRCm38)	V220I	possibly damaging	Het
Rnf170	T	C	8: 26,125,966 (GRCm38)	F95S	possibly damaging	Het
Sfpq	A	T	4: 127,021,348 (GRCm38)		probably null	Het
Slc9c1	A	T	16: 45,555,769 (GRCm38)	Y406F	probably damaging	Het
Stk32c	G	T	7: 139,120,712 (GRCm38)	Y200*	probably null	Het
Svil	A	G	18: 5,108,675 (GRCm38)	R1938G	probably damaging	Het
Sycp2	G	C	2: 178,348,245 (GRCm38)	R1403G	probably benign	Het
Tm9sf4	T	C	2: 153,182,489 (GRCm38)		probably null	Het
Tmc3	A	T	7: 83,614,962 (GRCm38)	M633L	possibly damaging	Het
Tmem163	T	C	1: 127,677,448 (GRCm38)	D61G	possibly damaging	Het
Unc5b	C	A	10: 60,777,546 (GRCm38)	A304S	probably benign	Het
Vmn2r106	G	A	17: 20,268,376 (GRCm38)	P587L	probably benign	Het
Vmn2r18	A	T	5: 151,584,997 (GRCm38)	S221T	possibly damaging	Het
Vmn2r85	T	G	10: 130,422,803 (GRCm38)	Y461S	probably damaging	Het
Yod1	T	C	1: 130,719,063 (GRCm38)	F226L	possibly damaging	Het
Zbtb4	T	C	11: 69,776,322 (GRCm38)	I151T	probably damaging	Het
Zfp110	A	T	7: 12,849,774 (GRCm38)	Q783L	probably damaging	Het

Other mutations in Scn9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Scn9a	APN	2	66,563,601 (GRCm38)	missense	probably damaging	1.00
IGL00570:Scn9a	APN	2	66,484,142 (GRCm38)	missense	probably damaging	1.00
IGL00809:Scn9a	APN	2	66,483,935 (GRCm38)	missense	probably damaging	1.00
IGL00977:Scn9a	APN	2	66,484,301 (GRCm38)	missense	probably damaging	0.99
IGL01120:Scn9a	APN	2	66,526,972 (GRCm38)	missense	probably benign	0.00
IGL01134:Scn9a	APN	2	66,504,968 (GRCm38)	missense	probably damaging	1.00
IGL01300:Scn9a	APN	2	66,488,053 (GRCm38)	nonsense	probably null
IGL01452:Scn9a	APN	2	66,527,072 (GRCm38)	missense	probably damaging	1.00
IGL01531:Scn9a	APN	2	66,537,378 (GRCm38)	missense	probably benign	0.11
IGL01572:Scn9a	APN	2	66,493,886 (GRCm38)	missense	probably benign	0.00
IGL01645:Scn9a	APN	2	66,487,642 (GRCm38)	missense	possibly damaging	0.62
IGL01823:Scn9a	APN	2	66,484,042 (GRCm38)	missense	probably damaging	1.00
IGL01965:Scn9a	APN	2	66,484,433 (GRCm38)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,494,826 (GRCm38)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,547,135 (GRCm38)	missense	probably damaging	1.00
IGL02166:Scn9a	APN	2	66,493,103 (GRCm38)	missense	possibly damaging	0.95
IGL02183:Scn9a	APN	2	66,484,611 (GRCm38)	splice site	probably benign
IGL02640:Scn9a	APN	2	66,536,096 (GRCm38)	critical splice donor site	probably null
IGL02685:Scn9a	APN	2	66,537,293 (GRCm38)	missense	probably damaging	1.00
IGL02798:Scn9a	APN	2	66,540,559 (GRCm38)	missense	possibly damaging	0.52
IGL02832:Scn9a	APN	2	66,568,029 (GRCm38)	missense	probably damaging	1.00
IGL03008:Scn9a	APN	2	66,562,511 (GRCm38)	missense	probably damaging	1.00
IGL03270:Scn9a	APN	2	66,484,014 (GRCm38)	missense	probably damaging	1.00
IGL03408:Scn9a	APN	2	66,526,747 (GRCm38)	missense	probably benign	0.00
BB007:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
BB017:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
R0039:Scn9a	UTSW	2	66,562,444 (GRCm38)	missense	probably damaging	0.98
R0173:Scn9a	UTSW	2	66,533,093 (GRCm38)	missense	probably damaging	1.00
R0323:Scn9a	UTSW	2	66,568,131 (GRCm38)	missense	probably damaging	1.00
R0344:Scn9a	UTSW	2	66,505,010 (GRCm38)	missense	probably damaging	0.99
R0421:Scn9a	UTSW	2	66,543,277 (GRCm38)	missense	probably benign
R0465:Scn9a	UTSW	2	66,526,996 (GRCm38)	missense	probably damaging	1.00
R0514:Scn9a	UTSW	2	66,483,678 (GRCm38)	missense	probably damaging	1.00
R0599:Scn9a	UTSW	2	66,526,799 (GRCm38)	missense	probably damaging	0.96
R0627:Scn9a	UTSW	2	66,537,377 (GRCm38)	missense	probably benign	0.00
R0644:Scn9a	UTSW	2	66,533,061 (GRCm38)	critical splice donor site	probably null
R0653:Scn9a	UTSW	2	66,533,377 (GRCm38)	missense	probably damaging	1.00
R0685:Scn9a	UTSW	2	66,483,499 (GRCm38)	missense	probably benign	0.02
R0718:Scn9a	UTSW	2	66,547,112 (GRCm38)	missense	probably damaging	1.00
R0827:Scn9a	UTSW	2	66,536,124 (GRCm38)	nonsense	probably null
R0890:Scn9a	UTSW	2	66,483,735 (GRCm38)	missense	probably damaging	1.00
R1139:Scn9a	UTSW	2	66,504,997 (GRCm38)	missense	probably benign	0.02
R1385:Scn9a	UTSW	2	66,563,542 (GRCm38)	missense	probably damaging	1.00
R1398:Scn9a	UTSW	2	66,484,586 (GRCm38)	missense	probably benign	0.11
R1496:Scn9a	UTSW	2	66,526,888 (GRCm38)	missense	probably benign
R1511:Scn9a	UTSW	2	66,526,813 (GRCm38)	missense	probably benign	0.01
R1517:Scn9a	UTSW	2	66,505,027 (GRCm38)	splice site	probably benign
R1564:Scn9a	UTSW	2	66,484,304 (GRCm38)	missense	probably damaging	1.00
R1634:Scn9a	UTSW	2	66,488,017 (GRCm38)	missense	probably damaging	1.00
R1662:Scn9a	UTSW	2	66,483,459 (GRCm38)	missense	probably benign	0.00
R1695:Scn9a	UTSW	2	66,504,876 (GRCm38)	nonsense	probably null
R1709:Scn9a	UTSW	2	66,483,506 (GRCm38)	missense	probably damaging	1.00
R1741:Scn9a	UTSW	2	66,487,594 (GRCm38)	missense	probably damaging	0.99
R1755:Scn9a	UTSW	2	66,501,716 (GRCm38)	missense	probably benign	0.38
R1914:Scn9a	UTSW	2	66,566,250 (GRCm38)	missense	probably damaging	1.00
R1962:Scn9a	UTSW	2	66,484,311 (GRCm38)	missense	probably damaging	1.00
R1970:Scn9a	UTSW	2	66,515,380 (GRCm38)	missense	probably damaging	0.97
R2017:Scn9a	UTSW	2	66,515,321 (GRCm38)	missense	probably damaging	0.99
R2092:Scn9a	UTSW	2	66,533,376 (GRCm38)	missense	probably damaging	0.99
R2105:Scn9a	UTSW	2	66,568,183 (GRCm38)	missense	probably benign	0.25
R2114:Scn9a	UTSW	2	66,484,052 (GRCm38)	missense	probably damaging	1.00
R2115:Scn9a	UTSW	2	66,484,052 (GRCm38)	missense	probably damaging	1.00
R2128:Scn9a	UTSW	2	66,526,654 (GRCm38)	missense	probably damaging	1.00
R2157:Scn9a	UTSW	2	66,536,325 (GRCm38)	missense	probably damaging	1.00
R2162:Scn9a	UTSW	2	66,534,229 (GRCm38)	missense	probably damaging	0.98
R2350:Scn9a	UTSW	2	66,504,968 (GRCm38)	missense	probably damaging	1.00
R3694:Scn9a	UTSW	2	66,562,405 (GRCm38)	missense	probably benign
R3771:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3772:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3773:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3922:Scn9a	UTSW	2	66,526,873 (GRCm38)	missense	possibly damaging	0.88
R3926:Scn9a	UTSW	2	66,526,873 (GRCm38)	missense	possibly damaging	0.88
R4258:Scn9a	UTSW	2	66,565,054 (GRCm38)	intron	probably benign
R4385:Scn9a	UTSW	2	66,484,556 (GRCm38)	missense	probably damaging	1.00
R4415:Scn9a	UTSW	2	66,526,693 (GRCm38)	missense	probably damaging	1.00
R4570:Scn9a	UTSW	2	66,483,558 (GRCm38)	missense	possibly damaging	0.85
R4682:Scn9a	UTSW	2	66,547,018 (GRCm38)	missense	probably benign
R4783:Scn9a	UTSW	2	66,540,623 (GRCm38)	missense	probably benign	0.01
R4822:Scn9a	UTSW	2	66,483,749 (GRCm38)	missense	possibly damaging	0.55
R4829:Scn9a	UTSW	2	66,551,713 (GRCm38)	missense	probably benign
R4908:Scn9a	UTSW	2	66,526,743 (GRCm38)	missense	probably benign	0.03
R4983:Scn9a	UTSW	2	66,566,270 (GRCm38)	missense	probably benign	0.02
R5047:Scn9a	UTSW	2	66,562,480 (GRCm38)	missense	probably damaging	1.00
R5100:Scn9a	UTSW	2	66,534,119 (GRCm38)	missense	probably damaging	1.00
R5140:Scn9a	UTSW	2	66,565,167 (GRCm38)	missense	possibly damaging	0.81
R5398:Scn9a	UTSW	2	66,488,043 (GRCm38)	missense	probably damaging	1.00
R5557:Scn9a	UTSW	2	66,547,103 (GRCm38)	missense	probably damaging	0.99
R5582:Scn9a	UTSW	2	66,565,029 (GRCm38)	intron	probably benign
R6108:Scn9a	UTSW	2	66,484,049 (GRCm38)	missense	probably damaging	1.00
R6143:Scn9a	UTSW	2	66,487,524 (GRCm38)	missense	probably benign	0.00
R6261:Scn9a	UTSW	2	66,483,896 (GRCm38)	missense	probably damaging	1.00
R6335:Scn9a	UTSW	2	66,568,264 (GRCm38)	start codon destroyed	possibly damaging	0.91
R6429:Scn9a	UTSW	2	66,526,963 (GRCm38)	missense	possibly damaging	0.95
R6632:Scn9a	UTSW	2	66,483,502 (GRCm38)	missense	probably benign	0.23
R6681:Scn9a	UTSW	2	66,563,342 (GRCm38)	missense	possibly damaging	0.90
R6830:Scn9a	UTSW	2	66,568,029 (GRCm38)	missense	probably damaging	1.00
R7102:Scn9a	UTSW	2	66,549,015 (GRCm38)	missense	probably damaging	1.00
R7186:Scn9a	UTSW	2	66,534,223 (GRCm38)	missense	probably damaging	1.00
R7243:Scn9a	UTSW	2	66,540,530 (GRCm38)	missense	probably damaging	1.00
R7311:Scn9a	UTSW	2	66,484,404 (GRCm38)	missense	possibly damaging	0.54
R7328:Scn9a	UTSW	2	66,484,587 (GRCm38)	missense	probably benign
R7386:Scn9a	UTSW	2	66,540,550 (GRCm38)	missense	probably damaging	1.00
R7438:Scn9a	UTSW	2	66,547,187 (GRCm38)	missense	possibly damaging	0.81
R7483:Scn9a	UTSW	2	66,533,348 (GRCm38)	missense	probably damaging	0.99
R7485:Scn9a	UTSW	2	66,534,217 (GRCm38)	missense	probably damaging	1.00
R7526:Scn9a	UTSW	2	66,483,646 (GRCm38)	missense	probably benign
R7617:Scn9a	UTSW	2	66,540,549 (GRCm38)	missense	possibly damaging	0.55
R7642:Scn9a	UTSW	2	66,536,236 (GRCm38)	missense	probably benign	0.02
R7653:Scn9a	UTSW	2	66,527,080 (GRCm38)	missense	probably damaging	1.00
R7747:Scn9a	UTSW	2	66,484,298 (GRCm38)	missense	probably damaging	1.00
R7823:Scn9a	UTSW	2	66,483,791 (GRCm38)	missense	probably damaging	1.00
R7864:Scn9a	UTSW	2	66,484,560 (GRCm38)	missense	possibly damaging	0.73
R7890:Scn9a	UTSW	2	66,543,112 (GRCm38)	missense	probably benign	0.00
R7930:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
R7975:Scn9a	UTSW	2	66,484,253 (GRCm38)	missense	probably damaging	1.00
R8057:Scn9a	UTSW	2	66,515,430 (GRCm38)	missense	probably benign	0.06
R8145:Scn9a	UTSW	2	66,487,410 (GRCm38)	missense	probably damaging	1.00
R8163:Scn9a	UTSW	2	66,484,401 (GRCm38)	missense	probably damaging	1.00
R8165:Scn9a	UTSW	2	66,540,530 (GRCm38)	missense	probably damaging	1.00
R8342:Scn9a	UTSW	2	66,536,282 (GRCm38)	missense	probably benign
R8345:Scn9a	UTSW	2	66,494,622 (GRCm38)	missense	probably damaging	0.96
R8464:Scn9a	UTSW	2	66,566,281 (GRCm38)	missense	probably damaging	0.99
R8467:Scn9a	UTSW	2	66,501,671 (GRCm38)	missense	probably damaging	1.00
R8698:Scn9a	UTSW	2	66,536,284 (GRCm38)	missense	probably benign	0.00
R8810:Scn9a	UTSW	2	66,501,666 (GRCm38)	missense	probably damaging	1.00
R8822:Scn9a	UTSW	2	66,540,635 (GRCm38)	missense	probably damaging	0.99
R8829:Scn9a	UTSW	2	66,483,617 (GRCm38)	missense	probably benign
R9009:Scn9a	UTSW	2	66,508,583 (GRCm38)	missense	probably damaging	1.00
R9038:Scn9a	UTSW	2	66,494,803 (GRCm38)	missense	probably damaging	1.00
R9126:Scn9a	UTSW	2	66,484,400 (GRCm38)	missense	probably damaging	1.00
R9205:Scn9a	UTSW	2	66,533,313 (GRCm38)	missense	probably damaging	1.00
R9300:Scn9a	UTSW	2	66,504,892 (GRCm38)	missense	probably benign	0.39
R9373:Scn9a	UTSW	2	66,483,917 (GRCm38)	missense	probably benign	0.00
R9404:Scn9a	UTSW	2	66,526,696 (GRCm38)	missense	probably benign	0.02
R9443:Scn9a	UTSW	2	66,565,209 (GRCm38)	missense	probably damaging	1.00
R9590:Scn9a	UTSW	2	66,483,984 (GRCm38)	missense	probably benign	0.05
R9612:Scn9a	UTSW	2	66,533,364 (GRCm38)	missense	probably damaging	1.00
R9617:Scn9a	UTSW	2	66,562,465 (GRCm38)	missense	probably damaging	1.00
R9717:Scn9a	UTSW	2	66,526,658 (GRCm38)	missense	probably benign
X0003:Scn9a	UTSW	2	66,508,647 (GRCm38)	missense	probably benign	0.02
X0062:Scn9a	UTSW	2	66,568,077 (GRCm38)	missense	probably damaging	1.00
Z1176:Scn9a	UTSW	2	66,540,592 (GRCm38)	missense	probably benign	0.00
Z1177:Scn9a	UTSW	2	66,494,685 (GRCm38)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- CCAGGTCCACAAACTCTGTC -3'
(R):5'- AGATGGATACCCTTCTCATGGTTC -3'

Sequencing Primer
(F):5'- CTCTGTCACATATCTGCAATAGGGG -3'
(R):5'- ATGCAACTCAGTGCTTTCTAGG -3'

Posted On

2017-08-16