Mutagenetix > Incidental Mutation

Incidental Mutation 'R6102:Aspm'

485284

Institutional Source

Beutler Lab

Gene Symbol

Aspm

Ensembl Gene

ENSMUSG00000033952

Gene Name

abnormal spindle microtubule assembly

Synonyms

Aspm, Sha1, MCPH5, D330028K02Rik, Calmbp1

MMRRC Submission

044252-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6102 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

139454772-139494091 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 139477459 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 1361 (Y1361*)

Ref Sequence

ENSEMBL: ENSMUSP00000059159 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053364] [ENSMUST00000200083]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000053364
AA Change: Y1361*

SMART Domains

Protein: ENSMUSP00000059159
Gene: ENSMUSG00000033952
AA Change: Y1361*

Domain	Start	End	E-Value	Type
Pfam:ASH	29	126	8.9e-35	PFAM
low complexity region	855	861	N/A	INTRINSIC
CH	890	1022	2.04e0	SMART
CH	1080	1224	5.56e-9	SMART
IQ	1233	1255	7.57e0	SMART
IQ	1259	1281	1.12e1	SMART
IQ	1282	1304	3.73e-1	SMART
IQ	1314	1336	2.41e-4	SMART
IQ	1360	1382	2.12e1	SMART
IQ	1387	1408	7.61e1	SMART
IQ	1409	1431	6.97e0	SMART
IQ	1432	1452	1.44e1	SMART
IQ	1453	1475	1.15e-1	SMART
IQ	1476	1495	1.66e2	SMART
IQ	1503	1525	1.65e-2	SMART
IQ	1526	1548	1.32e1	SMART
IQ	1549	1571	1.48e1	SMART
IQ	1572	1594	2.5e1	SMART
IQ	1599	1621	2.58e-4	SMART
IQ	1622	1644	6.7e-3	SMART
IQ	1645	1667	4.25e1	SMART
IQ	1668	1694	1.03e2	SMART
IQ	1695	1717	2.33e-2	SMART
IQ	1718	1740	7.79e0	SMART
IQ	1741	1763	1.57e2	SMART
IQ	1768	1790	2.68e-2	SMART
IQ	1791	1813	5.83e-3	SMART
IQ	1814	1836	5.93e1	SMART
IQ	1841	1863	1.92e-3	SMART
IQ	1864	1886	3.79e-2	SMART
IQ	1914	1936	4.11e0	SMART
IQ	1937	1959	1.87e-1	SMART
IQ	1960	1982	6.27e1	SMART
IQ	1987	2009	8.25e-3	SMART
IQ	2010	2032	5.73e0	SMART
IQ	2060	2082	1.39e0	SMART
IQ	2083	2105	4.62e1	SMART
IQ	2133	2155	5.58e0	SMART
IQ	2156	2178	7.07e-2	SMART
IQ	2206	2228	1.18e-3	SMART
IQ	2229	2251	4.59e0	SMART
IQ	2278	2300	1.85e-5	SMART
IQ	2301	2323	8.13e-2	SMART
IQ	2342	2364	9.62e-4	SMART
IQ	2365	2387	4.12e-3	SMART
IQ	2415	2437	7.58e-2	SMART
IQ	2438	2460	2.6e0	SMART
IQ	2490	2512	1.68e-3	SMART
IQ	2513	2535	8.51e1	SMART
IQ	2560	2582	2.14e-1	SMART
IQ	2601	2623	8.46e0	SMART
IQ	2647	2669	1.15e1	SMART
IQ	2673	2695	1.95e-4	SMART
IQ	2696	2718	4.13e1	SMART
IQ	2723	2745	1.02e-2	SMART
IQ	2761	2783	3.14e2	SMART
IQ	2784	2806	1e1	SMART
IQ	2825	2847	2.43e0	SMART
IQ	2848	2870	4.6e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199998

Predicted Effect

probably benign
Transcript: ENSMUST00000200083

SMART Domains

Protein: ENSMUSP00000142880
Gene: ENSMUSG00000033952

Domain	Start	End	E-Value	Type
low complexity region	855	861	N/A	INTRINSIC
CH	890	1022	2.04e0	SMART
CH	1080	1224	5.56e-9	SMART
IQ	1233	1255	7.57e0	SMART
IQ	1259	1281	1.12e1	SMART
IQ	1282	1304	3.73e-1	SMART
IQ	1314	1336	1.25e1	SMART
IQ	1337	1358	2.96e1	SMART
IQ	1382	1404	1.15e1	SMART
IQ	1408	1430	1.95e-4	SMART
IQ	1431	1453	4.13e1	SMART
IQ	1458	1480	1.02e-2	SMART
IQ	1496	1518	3.14e2	SMART
IQ	1519	1541	1e1	SMART
IQ	1560	1582	2.43e0	SMART
IQ	1583	1605	4.6e-1	SMART

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.4%
20x: 95.6%

Validation Efficiency

97% (65/67)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]
PHENOTYPE: Mice homozygous for protein-truncating gene trap mutations of this gene exhibit decreased body weight, microcephaly, a severe reduction in brain, testis and ovary weight, oligozoospermia and asthenospermia, and reduced fertility in both sexes. [provided by MGI curators]

Allele List at MGI

All alleles(9) : Targeted, other(2) Gene trapped(7)

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alox12e	A	G	11: 70,320,023 (GRCm38)	I290T	possibly damaging	Het
Apeh	T	A	9: 108,086,439 (GRCm38)	D559V	probably damaging	Het
Atad5	G	T	11: 80,111,572 (GRCm38)		probably null	Het
Ccr4	C	T	9: 114,496,493 (GRCm38)		probably null	Het
Clasrp	G	A	7: 19,586,468 (GRCm38)		probably benign	Het
Cldn23	A	G	8: 35,825,551 (GRCm38)	M261T	probably benign	Het
Cmya5	T	C	13: 93,094,231 (GRCm38)	M1450V	probably benign	Het
Cyp4v3	T	A	8: 45,320,160 (GRCm38)	E202V	probably damaging	Het
Dnah7a	A	T	1: 53,559,140 (GRCm38)	V1412E	probably benign	Het
Dnah9	A	G	11: 65,990,516 (GRCm38)	S2578P	probably damaging	Het
Dsc2	T	A	18: 20,047,108 (GRCm38)	Y196F	probably benign	Het
Exoc1	T	C	5: 76,537,779 (GRCm38)	S113P	probably damaging	Het
Fbxo6	A	T	4: 148,149,522 (GRCm38)	I39N	probably damaging	Het
Frs3	C	T	17: 47,702,671 (GRCm38)	H173Y	probably damaging	Het
Ginm1	G	T	10: 7,768,496 (GRCm38)	T323K	probably benign	Het
Golgb1	T	C	16: 36,912,865 (GRCm38)	S825P	probably damaging	Het
Hs3st3b1	A	T	11: 63,921,855 (GRCm38)	C11*	probably null	Het
Ighv3-3	G	C	12: 114,196,460 (GRCm38)	A110G	possibly damaging	Het
Igkv6-20	G	A	6: 70,335,869 (GRCm38)	H107Y	probably benign	Het
Kcnj16	A	G	11: 111,025,577 (GRCm38)	E355G	probably benign	Het
Lig4	C	T	8: 9,972,872 (GRCm38)	G303S	probably damaging	Het
Map1b	A	C	13: 99,425,873 (GRCm38)	V2443G	unknown	Het
Map3k6	T	C	4: 133,247,131 (GRCm38)		probably null	Het
Mmp13	A	G	9: 7,276,688 (GRCm38)	Q261R	probably benign	Het
Mphosph9	T	A	5: 124,297,709 (GRCm38)	I554F	possibly damaging	Het
Mrgpra3	A	T	7: 47,590,149 (GRCm38)	F10I	possibly damaging	Het
Ms4a18	G	A	19: 11,013,523 (GRCm38)	T69I	probably benign	Het
Mtmr3	T	C	11: 4,487,673 (GRCm38)	N927S	probably damaging	Het
Necab1	T	A	4: 14,989,211 (GRCm38)	Q188L	probably benign	Het
Nfatc2	A	G	2: 168,519,507 (GRCm38)		probably benign	Het
Olfr152	T	C	2: 87,782,848 (GRCm38)	F103L	probably damaging	Het
Olfr495	T	C	7: 108,395,284 (GRCm38)	S55P	probably damaging	Het
Olfr854	T	C	9: 19,567,022 (GRCm38)	M121V	possibly damaging	Het
Paip2b	A	C	6: 83,808,846 (GRCm38)	V134G	possibly damaging	Het
Pax3	T	C	1: 78,132,347 (GRCm38)	T225A	probably damaging	Het
Pbx1	C	A	1: 168,183,565 (GRCm38)	A298S	probably benign	Het
Pcdhac2	A	T	18: 37,146,282 (GRCm38)	K772*	probably null	Het
Pcsk4	A	T	10: 80,325,817 (GRCm38)	Y163*	probably null	Het
Plcd3	A	G	11: 103,080,644 (GRCm38)	V58A	probably damaging	Het
Plek2	T	A	12: 78,900,093 (GRCm38)	T57S	possibly damaging	Het
Plscr2	C	T	9: 92,287,668 (GRCm38)	T57I	probably benign	Het
Ppp2r5b	A	G	19: 6,234,738 (GRCm38)	S32P	probably benign	Het
Ppp3r2	C	T	4: 49,682,022 (GRCm38)		probably benign	Het
Psme4	T	A	11: 30,865,567 (GRCm38)	L1693H	probably damaging	Het
Rrbp1	T	A	2: 143,988,393 (GRCm38)	Q618L	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,579,904 (GRCm38)		probably benign	Het
Rsrc1	C	T	3: 66,994,649 (GRCm38)	P44L	unknown	Het
Serpina3b	A	T	12: 104,134,169 (GRCm38)	T337S	probably benign	Het
Slc18a2	T	C	19: 59,293,878 (GRCm38)	Y506H	probably benign	Het
Slc9a9	A	G	9: 94,936,429 (GRCm38)	Y292C	probably benign	Het
Spta1	T	C	1: 174,224,520 (GRCm38)	V1840A	probably benign	Het
Ssb	T	C	2: 69,871,208 (GRCm38)	*416Q	probably null	Het
Strada	A	G	11: 106,168,436 (GRCm38)	V209A	probably benign	Het
Tbc1d31	A	T	15: 57,936,093 (GRCm38)	D225V	probably damaging	Het
Tgm4	T	C	9: 123,056,535 (GRCm38)	F381L	probably benign	Het
Tmem120b	T	A	5: 123,115,144 (GRCm38)	Y203N	probably damaging	Het
Tmem176b	A	G	6: 48,835,934 (GRCm38)	V109A	probably benign	Het
Tnfrsf11a	C	A	1: 105,819,946 (GRCm38)	L201M	possibly damaging	Het
Ttn	C	A	2: 76,974,350 (GRCm38)		probably null	Het
Tubb2a	T	C	13: 34,075,343 (GRCm38)	I155V	probably benign	Het
Vmn1r5	A	G	6: 56,986,114 (GRCm38)	D258G	probably damaging	Het
Vmn2r121	T	C	X: 124,133,575 (GRCm38)	T120A	probably benign	Het
Vmn2r19	A	T	6: 123,329,948 (GRCm38)	I472F	probably damaging	Het
Vwa3a	A	G	7: 120,776,138 (GRCm38)		probably null	Het
Zfp273	A	G	13: 67,822,347 (GRCm38)	Y5C	probably damaging	Het
Zfp647	G	A	15: 76,912,085 (GRCm38)	P125L	probably damaging	Het
Zfp825	A	G	13: 74,480,653 (GRCm38)	L248P	probably damaging	Het

Other mutations in Aspm

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00489:Aspm	APN	1	139,478,691 (GRCm38)	missense	probably damaging	1.00
IGL00594:Aspm	APN	1	139,487,422 (GRCm38)	splice site	probably benign
IGL00808:Aspm	APN	1	139,461,476 (GRCm38)	missense	probably benign	0.03
IGL00897:Aspm	APN	1	139,477,407 (GRCm38)	missense	probably damaging	0.98
IGL01024:Aspm	APN	1	139,478,124 (GRCm38)	missense	possibly damaging	0.66
IGL01410:Aspm	APN	1	139,482,444 (GRCm38)	missense	probably benign	0.25
IGL01588:Aspm	APN	1	139,478,162 (GRCm38)	missense	probably benign	0.11
IGL01610:Aspm	APN	1	139,489,670 (GRCm38)	nonsense	probably null
IGL01633:Aspm	APN	1	139,480,836 (GRCm38)	missense	possibly damaging	0.93
IGL01982:Aspm	APN	1	139,491,588 (GRCm38)	missense	probably benign	0.12
IGL02429:Aspm	APN	1	139,479,810 (GRCm38)	missense	probably benign	0.27
IGL02468:Aspm	APN	1	139,480,950 (GRCm38)	missense	probably damaging	1.00
IGL02519:Aspm	APN	1	139,461,927 (GRCm38)	splice site	probably benign
IGL02526:Aspm	APN	1	139,489,719 (GRCm38)	missense	probably benign	0.03
IGL02716:Aspm	APN	1	139,479,687 (GRCm38)	missense	probably damaging	1.00
IGL02876:Aspm	APN	1	139,473,653 (GRCm38)	missense	probably damaging	1.00
IGL02953:Aspm	APN	1	139,457,419 (GRCm38)	missense	probably benign	0.01
IGL03275:Aspm	APN	1	139,487,295 (GRCm38)	missense	probably damaging	1.00
Stemware	UTSW	1	139,477,459 (GRCm38)	nonsense	probably null
3-1:Aspm	UTSW	1	139,457,541 (GRCm38)	missense	probably benign
R0016:Aspm	UTSW	1	139,479,544 (GRCm38)	missense	probably benign	0.01
R0016:Aspm	UTSW	1	139,479,544 (GRCm38)	missense	probably benign	0.01
R0106:Aspm	UTSW	1	139,476,876 (GRCm38)	missense	probably benign	0.02
R0106:Aspm	UTSW	1	139,476,876 (GRCm38)	missense	probably benign	0.02
R0140:Aspm	UTSW	1	139,480,641 (GRCm38)	missense	probably benign	0.00
R0195:Aspm	UTSW	1	139,479,135 (GRCm38)	missense	probably damaging	1.00
R0217:Aspm	UTSW	1	139,457,880 (GRCm38)	missense	possibly damaging	0.46
R0276:Aspm	UTSW	1	139,478,471 (GRCm38)	missense	possibly damaging	0.95
R0309:Aspm	UTSW	1	139,482,511 (GRCm38)	splice site	probably benign
R0466:Aspm	UTSW	1	139,477,901 (GRCm38)	missense	probably damaging	1.00
R0520:Aspm	UTSW	1	139,478,820 (GRCm38)	missense	possibly damaging	0.51
R0615:Aspm	UTSW	1	139,487,289 (GRCm38)	missense	probably damaging	1.00
R0626:Aspm	UTSW	1	139,491,601 (GRCm38)	missense	probably damaging	1.00
R0660:Aspm	UTSW	1	139,457,764 (GRCm38)	missense	probably benign	0.03
R0751:Aspm	UTSW	1	139,456,898 (GRCm38)	splice site	probably benign
R0830:Aspm	UTSW	1	139,474,254 (GRCm38)	missense	probably damaging	0.99
R1109:Aspm	UTSW	1	139,456,758 (GRCm38)	missense	probably damaging	0.99
R1114:Aspm	UTSW	1	139,461,924 (GRCm38)	splice site	probably benign
R1130:Aspm	UTSW	1	139,477,834 (GRCm38)	missense	possibly damaging	0.90
R1298:Aspm	UTSW	1	139,457,419 (GRCm38)	missense	probably benign	0.01
R1386:Aspm	UTSW	1	139,457,623 (GRCm38)	missense	probably benign	0.03
R1386:Aspm	UTSW	1	139,478,972 (GRCm38)	missense	possibly damaging	0.80
R1557:Aspm	UTSW	1	139,468,668 (GRCm38)	missense	probably benign	0.01
R1625:Aspm	UTSW	1	139,481,039 (GRCm38)	missense	probably benign	0.01
R1728:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1729:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1730:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1733:Aspm	UTSW	1	139,457,117 (GRCm38)	missense	probably benign	0.27
R1739:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1762:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1783:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1784:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1785:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1793:Aspm	UTSW	1	139,457,341 (GRCm38)	missense	probably benign	0.00
R1893:Aspm	UTSW	1	139,479,867 (GRCm38)	missense	probably damaging	1.00
R1911:Aspm	UTSW	1	139,478,094 (GRCm38)	missense	probably benign	0.06
R2103:Aspm	UTSW	1	139,491,665 (GRCm38)	missense	probably damaging	0.99
R2128:Aspm	UTSW	1	139,457,635 (GRCm38)	missense	probably benign	0.14
R2129:Aspm	UTSW	1	139,457,635 (GRCm38)	missense	probably benign	0.14
R2239:Aspm	UTSW	1	139,456,846 (GRCm38)	missense	possibly damaging	0.67
R2352:Aspm	UTSW	1	139,457,562 (GRCm38)	missense	probably benign	0.02
R2353:Aspm	UTSW	1	139,477,697 (GRCm38)	missense	probably damaging	1.00
R2380:Aspm	UTSW	1	139,479,348 (GRCm38)	missense	probably damaging	1.00
R2413:Aspm	UTSW	1	139,477,757 (GRCm38)	missense	probably damaging	1.00
R2421:Aspm	UTSW	1	139,488,487 (GRCm38)	missense	possibly damaging	0.49
R3607:Aspm	UTSW	1	139,480,668 (GRCm38)	missense	probably benign	0.13
R3711:Aspm	UTSW	1	139,458,100 (GRCm38)	missense	probably benign	0.17
R3718:Aspm	UTSW	1	139,490,427 (GRCm38)	missense	probably benign	0.31
R3718:Aspm	UTSW	1	139,480,889 (GRCm38)	missense	probably benign	0.09
R3741:Aspm	UTSW	1	139,478,619 (GRCm38)	missense	possibly damaging	0.47
R3788:Aspm	UTSW	1	139,463,203 (GRCm38)	missense	probably damaging	1.00
R3838:Aspm	UTSW	1	139,478,054 (GRCm38)	missense	probably benign	0.24
R3839:Aspm	UTSW	1	139,478,054 (GRCm38)	missense	probably benign	0.24
R3849:Aspm	UTSW	1	139,458,286 (GRCm38)	missense	probably benign	0.21
R4075:Aspm	UTSW	1	139,474,285 (GRCm38)	missense	probably damaging	1.00
R4080:Aspm	UTSW	1	139,470,755 (GRCm38)	missense	probably damaging	1.00
R4463:Aspm	UTSW	1	139,455,010 (GRCm38)	missense	possibly damaging	0.95
R4537:Aspm	UTSW	1	139,474,303 (GRCm38)	missense	probably benign	0.01
R4547:Aspm	UTSW	1	139,478,187 (GRCm38)	missense	possibly damaging	0.75
R4573:Aspm	UTSW	1	139,479,507 (GRCm38)	missense	probably damaging	0.98
R4680:Aspm	UTSW	1	139,480,671 (GRCm38)	missense	probably benign	0.05
R4807:Aspm	UTSW	1	139,477,919 (GRCm38)	missense	probably damaging	1.00
R4840:Aspm	UTSW	1	139,470,531 (GRCm38)	missense	possibly damaging	0.83
R4854:Aspm	UTSW	1	139,478,072 (GRCm38)	nonsense	probably null
R4859:Aspm	UTSW	1	139,469,393 (GRCm38)	missense	probably damaging	1.00
R4893:Aspm	UTSW	1	139,489,839 (GRCm38)	critical splice donor site	probably null
R4910:Aspm	UTSW	1	139,491,543 (GRCm38)	missense	probably damaging	1.00
R4953:Aspm	UTSW	1	139,471,734 (GRCm38)	missense	probably benign	0.00
R4974:Aspm	UTSW	1	139,478,010 (GRCm38)	missense	probably benign	0.03
R4981:Aspm	UTSW	1	139,470,760 (GRCm38)	splice site	probably null
R5082:Aspm	UTSW	1	139,478,676 (GRCm38)	nonsense	probably null
R5223:Aspm	UTSW	1	139,478,334 (GRCm38)	missense	probably damaging	1.00
R5268:Aspm	UTSW	1	139,464,295 (GRCm38)	missense	probably damaging	1.00
R5371:Aspm	UTSW	1	139,470,541 (GRCm38)	nonsense	probably null
R5377:Aspm	UTSW	1	139,470,395 (GRCm38)	splice site	probably null
R5377:Aspm	UTSW	1	139,457,483 (GRCm38)	missense	probably damaging	0.96
R5481:Aspm	UTSW	1	139,457,061 (GRCm38)	missense	possibly damaging	0.85
R5513:Aspm	UTSW	1	139,482,398 (GRCm38)	missense	probably damaging	1.00
R5578:Aspm	UTSW	1	139,470,717 (GRCm38)	missense	probably damaging	1.00
R5649:Aspm	UTSW	1	139,479,669 (GRCm38)	missense	probably benign
R5685:Aspm	UTSW	1	139,487,288 (GRCm38)	missense	probably benign	0.10
R5695:Aspm	UTSW	1	139,479,669 (GRCm38)	missense	probably benign
R5766:Aspm	UTSW	1	139,479,002 (GRCm38)	missense	probably damaging	0.99
R5964:Aspm	UTSW	1	139,455,227 (GRCm38)	intron	probably benign
R5993:Aspm	UTSW	1	139,479,531 (GRCm38)	missense	probably benign	0.28
R6027:Aspm	UTSW	1	139,463,056 (GRCm38)	missense	probably damaging	1.00
R6029:Aspm	UTSW	1	139,480,990 (GRCm38)	missense	possibly damaging	0.83
R6188:Aspm	UTSW	1	139,479,239 (GRCm38)	missense	possibly damaging	0.79
R6257:Aspm	UTSW	1	139,482,053 (GRCm38)	splice site	probably null
R6433:Aspm	UTSW	1	139,473,683 (GRCm38)	missense	probably damaging	1.00
R6682:Aspm	UTSW	1	139,457,722 (GRCm38)	missense	possibly damaging	0.67
R6763:Aspm	UTSW	1	139,470,517 (GRCm38)	missense	possibly damaging	0.64
R6798:Aspm	UTSW	1	139,468,685 (GRCm38)	missense	possibly damaging	0.66
R6815:Aspm	UTSW	1	139,480,142 (GRCm38)	missense	probably benign	0.04
R6854:Aspm	UTSW	1	139,463,182 (GRCm38)	missense	possibly damaging	0.90
R6928:Aspm	UTSW	1	139,480,206 (GRCm38)	nonsense	probably null
R6943:Aspm	UTSW	1	139,480,542 (GRCm38)	missense	probably damaging	1.00
R6979:Aspm	UTSW	1	139,480,485 (GRCm38)	missense	probably damaging	1.00
R6998:Aspm	UTSW	1	139,469,472 (GRCm38)	missense	probably damaging	1.00
R7126:Aspm	UTSW	1	139,480,803 (GRCm38)	missense	probably benign	0.27
R7237:Aspm	UTSW	1	139,477,929 (GRCm38)	missense	possibly damaging	0.81
R7240:Aspm	UTSW	1	139,478,651 (GRCm38)	nonsense	probably null
R7272:Aspm	UTSW	1	139,458,328 (GRCm38)	missense	probably benign	0.14
R7427:Aspm	UTSW	1	139,457,616 (GRCm38)	missense	probably benign	0.01
R7519:Aspm	UTSW	1	139,490,336 (GRCm38)	missense	possibly damaging	0.53
R7776:Aspm	UTSW	1	139,479,846 (GRCm38)	missense	possibly damaging	0.85
R7875:Aspm	UTSW	1	139,455,134 (GRCm38)	missense	probably benign	0.02
R7883:Aspm	UTSW	1	139,478,667 (GRCm38)	missense	possibly damaging	0.47
R7964:Aspm	UTSW	1	139,480,686 (GRCm38)	missense	probably damaging	1.00
R8012:Aspm	UTSW	1	139,457,464 (GRCm38)	missense	probably benign	0.03
R8029:Aspm	UTSW	1	139,471,632 (GRCm38)	missense	probably benign	0.00
R8233:Aspm	UTSW	1	139,457,304 (GRCm38)	missense	probably benign	0.28
R8277:Aspm	UTSW	1	139,455,010 (GRCm38)	missense	probably damaging	1.00
R8345:Aspm	UTSW	1	139,464,273 (GRCm38)	nonsense	probably null
R8491:Aspm	UTSW	1	139,457,695 (GRCm38)	missense	probably damaging	0.98
R8511:Aspm	UTSW	1	139,457,308 (GRCm38)	missense	probably damaging	1.00
R8557:Aspm	UTSW	1	139,456,756 (GRCm38)	missense	probably benign	0.01
R8927:Aspm	UTSW	1	139,490,387 (GRCm38)	nonsense	probably null
R8928:Aspm	UTSW	1	139,490,387 (GRCm38)	nonsense	probably null
R8950:Aspm	UTSW	1	139,478,952 (GRCm38)	missense	probably damaging	1.00
R9033:Aspm	UTSW	1	139,478,127 (GRCm38)	missense	probably damaging	1.00
R9083:Aspm	UTSW	1	139,493,698 (GRCm38)	missense	possibly damaging	0.70
R9133:Aspm	UTSW	1	139,491,528 (GRCm38)	missense	probably damaging	1.00
R9160:Aspm	UTSW	1	139,490,124 (GRCm38)	missense	probably damaging	1.00
R9179:Aspm	UTSW	1	139,476,715 (GRCm38)	missense	probably damaging	1.00
R9265:Aspm	UTSW	1	139,461,444 (GRCm38)	missense	probably benign	0.24
R9400:Aspm	UTSW	1	139,479,903 (GRCm38)	missense	probably damaging	1.00
R9419:Aspm	UTSW	1	139,457,185 (GRCm38)	missense	probably benign	0.29
R9454:Aspm	UTSW	1	139,480,994 (GRCm38)	missense	probably benign	0.00
R9517:Aspm	UTSW	1	139,479,429 (GRCm38)	missense	probably damaging	1.00
R9524:Aspm	UTSW	1	139,480,869 (GRCm38)	missense	probably damaging	1.00
R9544:Aspm	UTSW	1	139,457,785 (GRCm38)	missense	probably benign	0.01
R9640:Aspm	UTSW	1	139,480,272 (GRCm38)	missense	possibly damaging	0.88
R9698:Aspm	UTSW	1	139,461,908 (GRCm38)	missense	probably benign	0.28
R9790:Aspm	UTSW	1	139,480,637 (GRCm38)	missense	probably damaging	0.98
R9791:Aspm	UTSW	1	139,480,637 (GRCm38)	missense	probably damaging	0.98
R9794:Aspm	UTSW	1	139,478,742 (GRCm38)	missense	probably damaging	0.99
X0063:Aspm	UTSW	1	139,458,090 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GCTTGGTTATTTTAAAGCGGAATGC -3'
(R):5'- CTGAGATGCCATTCTCTAAAAGCTC -3'

Sequencing Primer
(F):5'- GCTAGATGAATGAGCATTTTCAAGTG -3'
(R):5'- AAAGCTCGCTGCAATGTTACTGC -3'

Posted On

2017-08-16