Mutagenetix > Incidental Mutation

Incidental Mutation 'R6108:Scn9a'

485587

Institutional Source

Beutler Lab

Gene Symbol

Scn9a

Ensembl Gene

ENSMUSG00000075316

Gene Name

sodium channel, voltage-gated, type IX, alpha

Synonyms

PN1

MMRRC Submission

044258-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6108 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66480080-66634962 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 66484049 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1764 (D1764G)

Ref Sequence

ENSEMBL: ENSMUSP00000131711 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100063] [ENSMUST00000100064] [ENSMUST00000112354] [ENSMUST00000164384] [ENSMUST00000169900]

AlphaFold

Q62205

Predicted Effect

probably damaging
Transcript: ENSMUST00000100063
AA Change: D1766G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097641
Gene: ENSMUSG00000075316
AA Change: D1766G

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	403	9.5e-78	PFAM
coiled coil region	404	442	N/A	INTRINSIC
Pfam:DUF3451	465	685	1.3e-62	PFAM
Pfam:Ion_trans	768	957	9.9e-48	PFAM
Pfam:Na_trans_assoc	972	1191	2.9e-72	PFAM
low complexity region	1203	1214	N/A	INTRINSIC
Pfam:Ion_trans	1217	1445	2.8e-55	PFAM
PDB:1BYY\|A	1447	1499	9e-27	PDB
Pfam:Ion_trans	1538	1748	3.4e-52	PFAM
Pfam:PKD_channel	1599	1755	1.1e-7	PFAM
IQ	1877	1899	1.03e-3	SMART
low complexity region	1956	1972	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000100064
AA Change: D1775G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097642
Gene: ENSMUSG00000075316
AA Change: D1775G

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	125	412	2.2e-84	PFAM
low complexity region	433	446	N/A	INTRINSIC
Pfam:Na_trans_cytopl	483	693	7.5e-76	PFAM
Pfam:Ion_trans	742	977	4.1e-57	PFAM
Pfam:Na_trans_assoc	981	1185	1.4e-58	PFAM
Pfam:Ion_trans	1189	1466	7e-67	PFAM
Pfam:Ion_trans	1512	1769	1e-55	PFAM
Pfam:PKD_channel	1605	1763	2.6e-7	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000112354
AA Change: D1764G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000107973
Gene: ENSMUSG00000075316
AA Change: D1764G

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.2e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141149

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152740

Predicted Effect

probably damaging
Transcript: ENSMUST00000164384
AA Change: D1775G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126528
Gene: ENSMUSG00000075316
AA Change: D1775G

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.1e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	694	4.2e-66	PFAM
Pfam:Ion_trans	777	966	8.8e-48	PFAM
Pfam:Na_trans_assoc	981	1200	6e-72	PFAM
low complexity region	1212	1223	N/A	INTRINSIC
Pfam:Ion_trans	1226	1454	2.5e-55	PFAM
PDB:1BYY\|A	1456	1508	6e-29	PDB
Pfam:Ion_trans	1547	1757	3e-52	PFAM
Pfam:PKD_channel	1608	1764	8.1e-8	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000169900
AA Change: D1764G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131711
Gene: ENSMUSG00000075316
AA Change: D1764G

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	3.7e-78	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Meta Mutation Damage Score

0.5721

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.8%

Validation Efficiency

96% (53/55)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal/neonatal lethality. Mice homozygous for a knock-in allele exhibit increased susceptibility to electrically induced seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	A	G	13: 81,391,695 (GRCm38)	F5702L	probably damaging	Het
Aptx	G	A	4: 40,694,986 (GRCm38)	Q117*	probably null	Het
Axin1	T	A	17: 26,143,240 (GRCm38)	M186K	probably damaging	Het
Btbd2	A	T	10: 80,645,531 (GRCm38)	L249Q	probably damaging	Het
Caprin1	C	T	2: 103,776,017 (GRCm38)	V293I	possibly damaging	Het
Ccdc136	C	A	6: 29,412,450 (GRCm38)	H334Q	probably benign	Het
Ccdc180	A	G	4: 45,911,389 (GRCm38)	N568S	possibly damaging	Het
Cenpf	A	T	1: 189,662,013 (GRCm38)	F515L	probably benign	Het
Chrm2	T	A	6: 36,523,295 (GRCm38)	V29E	probably damaging	Het
Cnot1	A	G	8: 95,730,420 (GRCm38)	L1956P	probably damaging	Het
Cyp2d22	T	C	15: 82,371,905 (GRCm38)	K176R	possibly damaging	Het
Dnah7a	A	T	1: 53,456,845 (GRCm38)	I3151N	probably damaging	Het
Dsg1a	T	A	18: 20,340,247 (GRCm38)	D792E	probably benign	Het
Fam167b	A	T	4: 129,578,308 (GRCm38)	L23*	probably null	Het
Fermt3	C	A	19: 7,014,414 (GRCm38)	R143L	probably benign	Het
Gfm2	A	G	13: 97,149,422 (GRCm38)	I140V	possibly damaging	Het
Gna14	A	G	19: 16,603,343 (GRCm38)	T182A	probably damaging	Het
Hmcn1	A	G	1: 150,631,227 (GRCm38)	V3738A	possibly damaging	Het
Hspa4	C	T	11: 53,261,712 (GRCm38)	G810D	probably damaging	Het
Igf2bp3	A	G	6: 49,117,374 (GRCm38)	I154T	probably damaging	Het
Il1rap	G	A	16: 26,722,707 (GRCm38)	S566N	probably damaging	Het
Kcnb1	G	A	2: 167,105,140 (GRCm38)	T596M	probably damaging	Het
Kcnn1	A	C	8: 70,855,156 (GRCm38)	S81A	probably benign	Het
Lmod1	G	A	1: 135,364,111 (GRCm38)	G235R	probably benign	Het
Mei1	A	T	15: 82,075,188 (GRCm38)	R185S	possibly damaging	Het
Mmrn1	G	A	6: 60,975,976 (GRCm38)	V414M	possibly damaging	Het
Mon2	C	A	10: 123,032,695 (GRCm38)	M484I	probably benign	Het
Nae1	A	T	8: 104,527,402 (GRCm38)	D99E	probably benign	Het
Nsun7	T	A	5: 66,295,799 (GRCm38)	I619N	probably damaging	Het
Nudt12	C	A	17: 59,007,749 (GRCm38)	R280L	probably damaging	Het
Olfr365	T	C	2: 37,201,766 (GRCm38)	V175A	possibly damaging	Het
P2ry1	T	A	3: 61,004,175 (GRCm38)	I245N	probably damaging	Het
Plch1	C	T	3: 63,702,023 (GRCm38)	R912H	probably damaging	Het
Plek	T	A	11: 16,990,058 (GRCm38)	Y217F	probably damaging	Het
Plpp1	A	T	13: 112,866,865 (GRCm38)	I208F	possibly damaging	Het
Ptges3-ps	C	T	6: 85,844,555 (GRCm38)		noncoding transcript	Het
Ptprf	A	G	4: 118,223,256 (GRCm38)	L1267P	probably benign	Het
Ptprz1	A	T	6: 23,045,659 (GRCm38)	S2143C	probably damaging	Het
Serpinb5	T	A	1: 106,881,728 (GRCm38)	L288Q	probably damaging	Het
Slc6a20b	T	G	9: 123,596,186 (GRCm38)	M539L	probably benign	Het
Slfnl1	A	G	4: 120,533,361 (GRCm38)	T70A	probably benign	Het
Smtn	C	A	11: 3,529,608 (GRCm38)	L486F	probably damaging	Het
Sptbn2	G	A	19: 4,731,392 (GRCm38)		probably null	Het
Tas2r144	C	A	6: 42,215,757 (GRCm38)	L144I	possibly damaging	Het
Tjp3	C	T	10: 81,281,146 (GRCm38)	R183K	probably benign	Het
Tnip3	A	G	6: 65,525,411 (GRCm38)		probably null	Het
Tspan12	T	A	6: 21,772,771 (GRCm38)	M212L	probably benign	Het
Ttn	T	A	2: 76,878,041 (GRCm38)		probably benign	Het
Vmn1r71	T	C	7: 10,748,618 (GRCm38)	M48V	probably benign	Het
Vmn2r10	A	G	5: 108,995,801 (GRCm38)	F761S	probably damaging	Het
Vmn2r106	G	A	17: 20,268,376 (GRCm38)	P587L	probably benign	Het
Wdr72	A	G	9: 74,151,668 (GRCm38)	T348A	probably damaging	Het
Xrn1	A	T	9: 95,974,427 (GRCm38)	L333F	possibly damaging	Het

Other mutations in Scn9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Scn9a	APN	2	66,563,601 (GRCm38)	missense	probably damaging	1.00
IGL00570:Scn9a	APN	2	66,484,142 (GRCm38)	missense	probably damaging	1.00
IGL00809:Scn9a	APN	2	66,483,935 (GRCm38)	missense	probably damaging	1.00
IGL00977:Scn9a	APN	2	66,484,301 (GRCm38)	missense	probably damaging	0.99
IGL01120:Scn9a	APN	2	66,526,972 (GRCm38)	missense	probably benign	0.00
IGL01134:Scn9a	APN	2	66,504,968 (GRCm38)	missense	probably damaging	1.00
IGL01300:Scn9a	APN	2	66,488,053 (GRCm38)	nonsense	probably null
IGL01452:Scn9a	APN	2	66,527,072 (GRCm38)	missense	probably damaging	1.00
IGL01531:Scn9a	APN	2	66,537,378 (GRCm38)	missense	probably benign	0.11
IGL01572:Scn9a	APN	2	66,493,886 (GRCm38)	missense	probably benign	0.00
IGL01645:Scn9a	APN	2	66,487,642 (GRCm38)	missense	possibly damaging	0.62
IGL01823:Scn9a	APN	2	66,484,042 (GRCm38)	missense	probably damaging	1.00
IGL01965:Scn9a	APN	2	66,484,433 (GRCm38)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,494,826 (GRCm38)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,547,135 (GRCm38)	missense	probably damaging	1.00
IGL02166:Scn9a	APN	2	66,493,103 (GRCm38)	missense	possibly damaging	0.95
IGL02183:Scn9a	APN	2	66,484,611 (GRCm38)	splice site	probably benign
IGL02640:Scn9a	APN	2	66,536,096 (GRCm38)	critical splice donor site	probably null
IGL02685:Scn9a	APN	2	66,537,293 (GRCm38)	missense	probably damaging	1.00
IGL02798:Scn9a	APN	2	66,540,559 (GRCm38)	missense	possibly damaging	0.52
IGL02832:Scn9a	APN	2	66,568,029 (GRCm38)	missense	probably damaging	1.00
IGL03008:Scn9a	APN	2	66,562,511 (GRCm38)	missense	probably damaging	1.00
IGL03270:Scn9a	APN	2	66,484,014 (GRCm38)	missense	probably damaging	1.00
IGL03408:Scn9a	APN	2	66,526,747 (GRCm38)	missense	probably benign	0.00
BB007:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
BB017:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
R0039:Scn9a	UTSW	2	66,562,444 (GRCm38)	missense	probably damaging	0.98
R0173:Scn9a	UTSW	2	66,533,093 (GRCm38)	missense	probably damaging	1.00
R0323:Scn9a	UTSW	2	66,568,131 (GRCm38)	missense	probably damaging	1.00
R0344:Scn9a	UTSW	2	66,505,010 (GRCm38)	missense	probably damaging	0.99
R0421:Scn9a	UTSW	2	66,543,277 (GRCm38)	missense	probably benign
R0465:Scn9a	UTSW	2	66,526,996 (GRCm38)	missense	probably damaging	1.00
R0514:Scn9a	UTSW	2	66,483,678 (GRCm38)	missense	probably damaging	1.00
R0599:Scn9a	UTSW	2	66,526,799 (GRCm38)	missense	probably damaging	0.96
R0627:Scn9a	UTSW	2	66,537,377 (GRCm38)	missense	probably benign	0.00
R0644:Scn9a	UTSW	2	66,533,061 (GRCm38)	critical splice donor site	probably null
R0653:Scn9a	UTSW	2	66,533,377 (GRCm38)	missense	probably damaging	1.00
R0685:Scn9a	UTSW	2	66,483,499 (GRCm38)	missense	probably benign	0.02
R0718:Scn9a	UTSW	2	66,547,112 (GRCm38)	missense	probably damaging	1.00
R0827:Scn9a	UTSW	2	66,536,124 (GRCm38)	nonsense	probably null
R0890:Scn9a	UTSW	2	66,483,735 (GRCm38)	missense	probably damaging	1.00
R1139:Scn9a	UTSW	2	66,504,997 (GRCm38)	missense	probably benign	0.02
R1385:Scn9a	UTSW	2	66,563,542 (GRCm38)	missense	probably damaging	1.00
R1398:Scn9a	UTSW	2	66,484,586 (GRCm38)	missense	probably benign	0.11
R1496:Scn9a	UTSW	2	66,526,888 (GRCm38)	missense	probably benign
R1511:Scn9a	UTSW	2	66,526,813 (GRCm38)	missense	probably benign	0.01
R1517:Scn9a	UTSW	2	66,505,027 (GRCm38)	splice site	probably benign
R1564:Scn9a	UTSW	2	66,484,304 (GRCm38)	missense	probably damaging	1.00
R1634:Scn9a	UTSW	2	66,488,017 (GRCm38)	missense	probably damaging	1.00
R1662:Scn9a	UTSW	2	66,483,459 (GRCm38)	missense	probably benign	0.00
R1695:Scn9a	UTSW	2	66,504,876 (GRCm38)	nonsense	probably null
R1709:Scn9a	UTSW	2	66,483,506 (GRCm38)	missense	probably damaging	1.00
R1741:Scn9a	UTSW	2	66,487,594 (GRCm38)	missense	probably damaging	0.99
R1755:Scn9a	UTSW	2	66,501,716 (GRCm38)	missense	probably benign	0.38
R1914:Scn9a	UTSW	2	66,566,250 (GRCm38)	missense	probably damaging	1.00
R1962:Scn9a	UTSW	2	66,484,311 (GRCm38)	missense	probably damaging	1.00
R1970:Scn9a	UTSW	2	66,515,380 (GRCm38)	missense	probably damaging	0.97
R2017:Scn9a	UTSW	2	66,515,321 (GRCm38)	missense	probably damaging	0.99
R2092:Scn9a	UTSW	2	66,533,376 (GRCm38)	missense	probably damaging	0.99
R2105:Scn9a	UTSW	2	66,568,183 (GRCm38)	missense	probably benign	0.25
R2114:Scn9a	UTSW	2	66,484,052 (GRCm38)	missense	probably damaging	1.00
R2115:Scn9a	UTSW	2	66,484,052 (GRCm38)	missense	probably damaging	1.00
R2128:Scn9a	UTSW	2	66,526,654 (GRCm38)	missense	probably damaging	1.00
R2157:Scn9a	UTSW	2	66,536,325 (GRCm38)	missense	probably damaging	1.00
R2162:Scn9a	UTSW	2	66,534,229 (GRCm38)	missense	probably damaging	0.98
R2350:Scn9a	UTSW	2	66,504,968 (GRCm38)	missense	probably damaging	1.00
R3694:Scn9a	UTSW	2	66,562,405 (GRCm38)	missense	probably benign
R3771:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3772:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3773:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3922:Scn9a	UTSW	2	66,526,873 (GRCm38)	missense	possibly damaging	0.88
R3926:Scn9a	UTSW	2	66,526,873 (GRCm38)	missense	possibly damaging	0.88
R4258:Scn9a	UTSW	2	66,565,054 (GRCm38)	intron	probably benign
R4385:Scn9a	UTSW	2	66,484,556 (GRCm38)	missense	probably damaging	1.00
R4415:Scn9a	UTSW	2	66,526,693 (GRCm38)	missense	probably damaging	1.00
R4570:Scn9a	UTSW	2	66,483,558 (GRCm38)	missense	possibly damaging	0.85
R4682:Scn9a	UTSW	2	66,547,018 (GRCm38)	missense	probably benign
R4783:Scn9a	UTSW	2	66,540,623 (GRCm38)	missense	probably benign	0.01
R4822:Scn9a	UTSW	2	66,483,749 (GRCm38)	missense	possibly damaging	0.55
R4829:Scn9a	UTSW	2	66,551,713 (GRCm38)	missense	probably benign
R4908:Scn9a	UTSW	2	66,526,743 (GRCm38)	missense	probably benign	0.03
R4983:Scn9a	UTSW	2	66,566,270 (GRCm38)	missense	probably benign	0.02
R5047:Scn9a	UTSW	2	66,562,480 (GRCm38)	missense	probably damaging	1.00
R5100:Scn9a	UTSW	2	66,534,119 (GRCm38)	missense	probably damaging	1.00
R5140:Scn9a	UTSW	2	66,565,167 (GRCm38)	missense	possibly damaging	0.81
R5398:Scn9a	UTSW	2	66,488,043 (GRCm38)	missense	probably damaging	1.00
R5557:Scn9a	UTSW	2	66,547,103 (GRCm38)	missense	probably damaging	0.99
R5582:Scn9a	UTSW	2	66,565,029 (GRCm38)	intron	probably benign
R6115:Scn9a	UTSW	2	66,563,629 (GRCm38)	missense	possibly damaging	0.70
R6143:Scn9a	UTSW	2	66,487,524 (GRCm38)	missense	probably benign	0.00
R6261:Scn9a	UTSW	2	66,483,896 (GRCm38)	missense	probably damaging	1.00
R6335:Scn9a	UTSW	2	66,568,264 (GRCm38)	start codon destroyed	possibly damaging	0.91
R6429:Scn9a	UTSW	2	66,526,963 (GRCm38)	missense	possibly damaging	0.95
R6632:Scn9a	UTSW	2	66,483,502 (GRCm38)	missense	probably benign	0.23
R6681:Scn9a	UTSW	2	66,563,342 (GRCm38)	missense	possibly damaging	0.90
R6830:Scn9a	UTSW	2	66,568,029 (GRCm38)	missense	probably damaging	1.00
R7102:Scn9a	UTSW	2	66,549,015 (GRCm38)	missense	probably damaging	1.00
R7186:Scn9a	UTSW	2	66,534,223 (GRCm38)	missense	probably damaging	1.00
R7243:Scn9a	UTSW	2	66,540,530 (GRCm38)	missense	probably damaging	1.00
R7311:Scn9a	UTSW	2	66,484,404 (GRCm38)	missense	possibly damaging	0.54
R7328:Scn9a	UTSW	2	66,484,587 (GRCm38)	missense	probably benign
R7386:Scn9a	UTSW	2	66,540,550 (GRCm38)	missense	probably damaging	1.00
R7438:Scn9a	UTSW	2	66,547,187 (GRCm38)	missense	possibly damaging	0.81
R7483:Scn9a	UTSW	2	66,533,348 (GRCm38)	missense	probably damaging	0.99
R7485:Scn9a	UTSW	2	66,534,217 (GRCm38)	missense	probably damaging	1.00
R7526:Scn9a	UTSW	2	66,483,646 (GRCm38)	missense	probably benign
R7617:Scn9a	UTSW	2	66,540,549 (GRCm38)	missense	possibly damaging	0.55
R7642:Scn9a	UTSW	2	66,536,236 (GRCm38)	missense	probably benign	0.02
R7653:Scn9a	UTSW	2	66,527,080 (GRCm38)	missense	probably damaging	1.00
R7747:Scn9a	UTSW	2	66,484,298 (GRCm38)	missense	probably damaging	1.00
R7823:Scn9a	UTSW	2	66,483,791 (GRCm38)	missense	probably damaging	1.00
R7864:Scn9a	UTSW	2	66,484,560 (GRCm38)	missense	possibly damaging	0.73
R7890:Scn9a	UTSW	2	66,543,112 (GRCm38)	missense	probably benign	0.00
R7930:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
R7975:Scn9a	UTSW	2	66,484,253 (GRCm38)	missense	probably damaging	1.00
R8057:Scn9a	UTSW	2	66,515,430 (GRCm38)	missense	probably benign	0.06
R8145:Scn9a	UTSW	2	66,487,410 (GRCm38)	missense	probably damaging	1.00
R8163:Scn9a	UTSW	2	66,484,401 (GRCm38)	missense	probably damaging	1.00
R8165:Scn9a	UTSW	2	66,540,530 (GRCm38)	missense	probably damaging	1.00
R8342:Scn9a	UTSW	2	66,536,282 (GRCm38)	missense	probably benign
R8345:Scn9a	UTSW	2	66,494,622 (GRCm38)	missense	probably damaging	0.96
R8464:Scn9a	UTSW	2	66,566,281 (GRCm38)	missense	probably damaging	0.99
R8467:Scn9a	UTSW	2	66,501,671 (GRCm38)	missense	probably damaging	1.00
R8698:Scn9a	UTSW	2	66,536,284 (GRCm38)	missense	probably benign	0.00
R8810:Scn9a	UTSW	2	66,501,666 (GRCm38)	missense	probably damaging	1.00
R8822:Scn9a	UTSW	2	66,540,635 (GRCm38)	missense	probably damaging	0.99
R8829:Scn9a	UTSW	2	66,483,617 (GRCm38)	missense	probably benign
R9009:Scn9a	UTSW	2	66,508,583 (GRCm38)	missense	probably damaging	1.00
R9038:Scn9a	UTSW	2	66,494,803 (GRCm38)	missense	probably damaging	1.00
R9126:Scn9a	UTSW	2	66,484,400 (GRCm38)	missense	probably damaging	1.00
R9205:Scn9a	UTSW	2	66,533,313 (GRCm38)	missense	probably damaging	1.00
R9300:Scn9a	UTSW	2	66,504,892 (GRCm38)	missense	probably benign	0.39
R9373:Scn9a	UTSW	2	66,483,917 (GRCm38)	missense	probably benign	0.00
R9404:Scn9a	UTSW	2	66,526,696 (GRCm38)	missense	probably benign	0.02
R9443:Scn9a	UTSW	2	66,565,209 (GRCm38)	missense	probably damaging	1.00
R9590:Scn9a	UTSW	2	66,483,984 (GRCm38)	missense	probably benign	0.05
R9612:Scn9a	UTSW	2	66,533,364 (GRCm38)	missense	probably damaging	1.00
R9617:Scn9a	UTSW	2	66,562,465 (GRCm38)	missense	probably damaging	1.00
R9717:Scn9a	UTSW	2	66,526,658 (GRCm38)	missense	probably benign
X0003:Scn9a	UTSW	2	66,508,647 (GRCm38)	missense	probably benign	0.02
X0062:Scn9a	UTSW	2	66,568,077 (GRCm38)	missense	probably damaging	1.00
Z1176:Scn9a	UTSW	2	66,540,592 (GRCm38)	missense	probably benign	0.00
Z1177:Scn9a	UTSW	2	66,494,685 (GRCm38)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- GAAGGGAATCCATCTCTCCG -3'
(R):5'- TTCACCCAGGAAGTTCAGTG -3'

Sequencing Primer
(F):5'- CAGGACCCGCTTTGTAAAAG -3'
(R):5'- CACCCAGGAAGTTCAGTGGAAGG -3'

Posted On

2017-08-16