Mutagenetix > Incidental Mutation

Incidental Mutation 'R6033:Unc80'

486366

Institutional Source

Beutler Lab

Gene Symbol

Unc80

Ensembl Gene

ENSMUSG00000055567

Gene Name

unc-80, NALCN activator

Synonyms

C030018G13Rik, C230061B10Rik

MMRRC Submission

044205-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.920) question?

Stock #

R6033 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66468367-66699148 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 66473260 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 110 (T110S)

Ref Sequence

ENSEMBL: ENSMUSP00000109641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061620] [ENSMUST00000114008] [ENSMUST00000212557]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000061620
AA Change: T110S

PolyPhen 2 Score 0.463 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000053692
Gene: ENSMUSG00000055567
AA Change: T110S

Domain	Start	End	E-Value	Type
Pfam:UNC80	16	236	2.2e-94	PFAM
low complexity region	372	385	N/A	INTRINSIC
low complexity region	493	502	N/A	INTRINSIC
low complexity region	693	711	N/A	INTRINSIC
low complexity region	723	738	N/A	INTRINSIC
low complexity region	739	769	N/A	INTRINSIC
low complexity region	1038	1055	N/A	INTRINSIC
low complexity region	1067	1084	N/A	INTRINSIC
low complexity region	1309	1328	N/A	INTRINSIC
low complexity region	1477	1489	N/A	INTRINSIC
low complexity region	1676	1681	N/A	INTRINSIC
low complexity region	1842	1848	N/A	INTRINSIC
low complexity region	1854	1868	N/A	INTRINSIC
low complexity region	2461	2480	N/A	INTRINSIC
low complexity region	2726	2740	N/A	INTRINSIC
low complexity region	3121	3144	N/A	INTRINSIC
low complexity region	3245	3254	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114008
AA Change: T110S

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136727

Predicted Effect

possibly damaging
Transcript: ENSMUST00000212557
AA Change: T110S

PolyPhen 2 Score 0.655 (Sensitivity: 0.87; Specificity: 0.91)

Meta Mutation Damage Score

0.1133

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.0%

Validation Efficiency

100% (55/55)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of a voltage-independent 'leak' ion-channel complex, in which it performs essential functions, such as serving as a bridge between two other components (sodium leak channel non-selective and UNC79) and as a scaffold for Src kinases. Leak channels play an importnat role in establishment and maintenance of resting membrane potentials in neurons. Mutations in this gene are associated with congenital infantile encephalopathy, intellectual disability and growth issues. [provided by RefSeq, Aug 2016]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl1	T	A	8: 83,918,922 (GRCm38)	V58E	probably damaging	Het
Afg3l2	G	T	18: 67,421,259 (GRCm38)	L458M	probably damaging	Het
Alas1	A	G	9: 106,241,204 (GRCm38)	S240P	probably damaging	Het
Alox12e	C	T	11: 70,316,013 (GRCm38)	G616D	probably benign	Het
Arhgap8	A	G	15: 84,741,925 (GRCm38)	E67G	probably damaging	Het
Ash1l	T	C	3: 88,985,019 (GRCm38)	Y1402H	probably damaging	Het
Ccdc150	G	T	1: 54,285,628 (GRCm38)		probably null	Het
Chct1	A	G	11: 85,178,372 (GRCm38)	E72G	probably damaging	Het
Cmtm8	T	C	9: 114,796,073 (GRCm38)	T97A	probably damaging	Het
Cnmd	T	C	14: 79,661,505 (GRCm38)	S36G	probably benign	Het
Dnah3	A	C	7: 120,071,647 (GRCm38)	N609K	probably benign	Het
Dph1	C	T	11: 75,191,197 (GRCm38)		probably benign	Het
Drosha	G	A	15: 12,925,999 (GRCm38)	A1225T	probably benign	Het
Eid3	T	A	10: 82,867,653 (GRCm38)	I316K	probably damaging	Het
Erich6	A	G	3: 58,623,201 (GRCm38)	L449S	probably benign	Het
Fhod1	T	C	8: 105,336,434 (GRCm38)		probably benign	Het
Glra1	A	G	11: 55,527,419 (GRCm38)	Y250H	probably damaging	Het
Gm21972	T	C	1: 86,137,095 (GRCm38)	Y950H	probably damaging	Het
Gm6712	T	A	17: 17,294,416 (GRCm38)		noncoding transcript	Het
Grb7	C	T	11: 98,455,197 (GRCm38)		probably null	Het
Hivep1	A	G	13: 42,157,107 (GRCm38)	E941G	probably benign	Het
Homer2	A	C	7: 81,618,679 (GRCm38)	S78A	possibly damaging	Het
Ica1	T	A	6: 8,630,799 (GRCm38)		probably null	Het
Ifna12	T	C	4: 88,602,917 (GRCm38)	E131G	possibly damaging	Het
Igbp1b	T	C	6: 138,658,209 (GRCm38)	Y79C	probably damaging	Het
Incenp	C	T	19: 9,872,697 (GRCm38)	V871I	probably damaging	Het
Jaml	G	A	9: 45,088,710 (GRCm38)	G60D	probably damaging	Het
Kcp	C	T	6: 29,493,194 (GRCm38)	C110Y	probably damaging	Het
Manba	T	C	3: 135,549,261 (GRCm38)	V460A	probably benign	Het
Myrfl	A	T	10: 116,849,101 (GRCm38)	C125S	probably benign	Het
Ncan	T	C	8: 70,112,590 (GRCm38)	D229G	probably damaging	Het
Ncoa4-ps	A	G	12: 119,261,740 (GRCm38)		noncoding transcript	Het
Nlrp10	A	T	7: 108,924,577 (GRCm38)	D565E	probably benign	Het
Npas2	T	C	1: 39,338,180 (GRCm38)	V541A	probably damaging	Het
Nsg2	G	A	11: 32,055,058 (GRCm38)	V87M	possibly damaging	Het
Or5al6	A	T	2: 86,146,269 (GRCm38)	V155E	probably damaging	Het
Prkd2	C	T	7: 16,865,714 (GRCm38)	R701C	probably damaging	Het
Prr3	A	T	17: 35,978,624 (GRCm38)		probably null	Het
Prss36	T	C	7: 127,934,567 (GRCm38)	R22G	probably benign	Het
Slc45a4	G	A	15: 73,581,976 (GRCm38)	A716V	probably damaging	Het
Slc46a1	T	C	11: 78,466,007 (GRCm38)		probably null	Het
Slc6a5	T	C	7: 49,959,351 (GRCm38)	I768T	probably benign	Het
Slco6c1	C	T	1: 97,081,316 (GRCm38)		probably null	Het
Taar2	A	T	10: 23,940,976 (GRCm38)	H138L	probably benign	Het
Taf2	A	C	15: 55,058,901 (GRCm38)	L330R	probably damaging	Het
Tgm5	T	C	2: 121,070,729 (GRCm38)		probably null	Het
Tmed4	A	T	11: 6,274,491 (GRCm38)	Y56*	probably null	Het
Tmem156	A	T	5: 65,075,621 (GRCm38)	F135L	probably benign	Het
Ttll6	T	C	11: 96,134,887 (GRCm38)	S65P	probably damaging	Het
Ttn	C	T	2: 76,726,827 (GRCm38)	G28199R	probably damaging	Het
Ubn2	T	A	6: 38,470,224 (GRCm38)		probably null	Het
Vmn2r72	A	T	7: 85,737,929 (GRCm38)	V809E	probably damaging	Het
Zbtb2	A	G	10: 4,368,599 (GRCm38)	F476L	probably damaging	Het
Zbtb24	T	C	10: 41,464,401 (GRCm38)	F498L	probably damaging	Het
Zfp280d	T	A	9: 72,329,137 (GRCm38)	L494Q	probably damaging	Het
Zfp281	T	C	1: 136,626,726 (GRCm38)	S481P	probably benign	Het

Other mutations in Unc80

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00160:Unc80	APN	1	66,654,395 (GRCm38)	missense	possibly damaging	0.53
IGL00340:Unc80	APN	1	66,606,459 (GRCm38)	missense	possibly damaging	0.73
IGL00783:Unc80	APN	1	66,608,437 (GRCm38)	missense	probably benign	0.37
IGL00784:Unc80	APN	1	66,608,437 (GRCm38)	missense	probably benign	0.37
IGL00935:Unc80	APN	1	66,627,266 (GRCm38)	missense	possibly damaging	0.53
IGL01094:Unc80	APN	1	66,695,433 (GRCm38)	missense	possibly damaging	0.90
IGL01466:Unc80	APN	1	66,622,486 (GRCm38)	missense	probably benign	0.33
IGL01577:Unc80	APN	1	66,529,968 (GRCm38)	splice site	probably null
IGL01626:Unc80	APN	1	66,551,054 (GRCm38)	critical splice donor site	probably null
IGL01640:Unc80	APN	1	66,679,585 (GRCm38)	missense	probably benign	0.33
IGL01775:Unc80	APN	1	66,601,056 (GRCm38)	missense	possibly damaging	0.94
IGL01960:Unc80	APN	1	66,608,500 (GRCm38)	splice site	probably benign
IGL01991:Unc80	APN	1	66,469,509 (GRCm38)	nonsense	probably null
IGL02022:Unc80	APN	1	66,626,516 (GRCm38)	missense	possibly damaging	0.53
IGL02073:Unc80	APN	1	66,612,227 (GRCm38)	missense	possibly damaging	0.85
IGL02077:Unc80	APN	1	66,525,716 (GRCm38)	missense	possibly damaging	0.77
IGL02197:Unc80	APN	1	66,530,065 (GRCm38)	missense	probably benign	0.39
IGL02198:Unc80	APN	1	66,529,986 (GRCm38)	missense	possibly damaging	0.88
IGL02228:Unc80	APN	1	66,608,428 (GRCm38)	missense	possibly damaging	0.72
IGL02327:Unc80	APN	1	66,641,673 (GRCm38)	missense	probably benign	0.33
IGL02447:Unc80	APN	1	66,503,544 (GRCm38)	missense	possibly damaging	0.86
IGL02489:Unc80	APN	1	66,525,701 (GRCm38)	missense	probably benign	0.07
IGL02546:Unc80	APN	1	66,554,953 (GRCm38)	missense	possibly damaging	0.83
IGL02629:Unc80	APN	1	66,483,317 (GRCm38)	missense	possibly damaging	0.46
IGL02631:Unc80	APN	1	66,530,063 (GRCm38)	missense	probably damaging	0.98
IGL02839:Unc80	APN	1	66,671,675 (GRCm38)	missense	possibly damaging	0.53
IGL02960:Unc80	APN	1	66,678,058 (GRCm38)	splice site	probably benign
IGL02974:Unc80	APN	1	66,525,658 (GRCm38)	missense	possibly damaging	0.95
IGL03060:Unc80	APN	1	66,637,010 (GRCm38)	missense	possibly damaging	0.96
IGL03062:Unc80	APN	1	66,509,489 (GRCm38)	missense	probably damaging	0.96
IGL03074:Unc80	APN	1	66,671,718 (GRCm38)	splice site	probably benign
IGL03086:Unc80	APN	1	66,509,474 (GRCm38)	missense	probably damaging	0.99
IGL03105:Unc80	APN	1	66,472,099 (GRCm38)	missense	probably damaging	0.96
IGL03107:Unc80	APN	1	66,631,454 (GRCm38)	missense	probably damaging	0.98
IGL03158:Unc80	APN	1	66,641,674 (GRCm38)	missense	probably benign	0.33
IGL03220:Unc80	APN	1	66,504,938 (GRCm38)	missense	probably damaging	0.99
IGL03271:Unc80	APN	1	66,695,603 (GRCm38)	unclassified	probably benign
IGL03332:Unc80	APN	1	66,503,631 (GRCm38)	missense	probably damaging	1.00
IGL03347:Unc80	APN	1	66,695,466 (GRCm38)	missense	probably damaging	1.00
R0012:Unc80	UTSW	1	66,507,391 (GRCm38)	missense	probably damaging	1.00
R0012:Unc80	UTSW	1	66,507,391 (GRCm38)	missense	probably damaging	1.00
R0026:Unc80	UTSW	1	66,521,584 (GRCm38)	missense	probably benign	0.27
R0055:Unc80	UTSW	1	66,506,623 (GRCm38)	splice site	probably benign
R0149:Unc80	UTSW	1	66,521,601 (GRCm38)	missense	possibly damaging	0.82
R0325:Unc80	UTSW	1	66,510,881 (GRCm38)	missense	probably damaging	1.00
R0329:Unc80	UTSW	1	66,674,087 (GRCm38)	missense	possibly damaging	0.96
R0330:Unc80	UTSW	1	66,674,087 (GRCm38)	missense	possibly damaging	0.96
R0355:Unc80	UTSW	1	66,549,856 (GRCm38)	missense	possibly damaging	0.77
R0412:Unc80	UTSW	1	66,550,937 (GRCm38)	splice site	probably benign
R0422:Unc80	UTSW	1	66,483,338 (GRCm38)	missense	probably damaging	1.00
R0477:Unc80	UTSW	1	66,570,001 (GRCm38)	missense	probably damaging	0.99
R0507:Unc80	UTSW	1	66,527,893 (GRCm38)	missense	possibly damaging	0.66
R0513:Unc80	UTSW	1	66,622,474 (GRCm38)	missense	possibly damaging	0.73
R0553:Unc80	UTSW	1	66,506,669 (GRCm38)	missense	probably damaging	0.97
R0626:Unc80	UTSW	1	66,608,442 (GRCm38)	missense	probably benign	0.01
R0655:Unc80	UTSW	1	66,503,781 (GRCm38)	missense	probably damaging	0.98
R0742:Unc80	UTSW	1	66,527,893 (GRCm38)	missense	possibly damaging	0.66
R0755:Unc80	UTSW	1	66,504,923 (GRCm38)	missense	probably damaging	1.00
R0782:Unc80	UTSW	1	66,622,581 (GRCm38)	missense	possibly damaging	0.53
R0837:Unc80	UTSW	1	66,648,944 (GRCm38)	missense	possibly damaging	0.73
R0841:Unc80	UTSW	1	66,472,088 (GRCm38)	missense	probably damaging	1.00
R0893:Unc80	UTSW	1	66,521,486 (GRCm38)	missense	probably damaging	0.97
R0900:Unc80	UTSW	1	66,671,598 (GRCm38)	missense	probably benign	0.33
R0924:Unc80	UTSW	1	66,510,641 (GRCm38)	missense	possibly damaging	0.95
R0930:Unc80	UTSW	1	66,510,641 (GRCm38)	missense	possibly damaging	0.95
R0989:Unc80	UTSW	1	66,646,440 (GRCm38)	missense	possibly damaging	0.53
R1145:Unc80	UTSW	1	66,472,088 (GRCm38)	missense	probably damaging	1.00
R1145:Unc80	UTSW	1	66,472,088 (GRCm38)	missense	probably damaging	1.00
R1224:Unc80	UTSW	1	66,471,980 (GRCm38)	missense	probably damaging	1.00
R1240:Unc80	UTSW	1	66,635,902 (GRCm38)	missense	possibly damaging	0.85
R1245:Unc80	UTSW	1	66,555,095 (GRCm38)	missense	possibly damaging	0.94
R1467:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1473:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1500:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1556:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1562:Unc80	UTSW	1	66,637,957 (GRCm38)	missense	probably damaging	1.00
R1655:Unc80	UTSW	1	66,672,756 (GRCm38)	missense	possibly damaging	0.86
R1674:Unc80	UTSW	1	66,509,308 (GRCm38)	missense	probably damaging	1.00
R1680:Unc80	UTSW	1	66,503,669 (GRCm38)	nonsense	probably null
R1739:Unc80	UTSW	1	66,527,892 (GRCm38)	missense	probably damaging	0.97
R1756:Unc80	UTSW	1	66,639,248 (GRCm38)	missense	possibly damaging	0.53
R1783:Unc80	UTSW	1	66,683,273 (GRCm38)	missense	probably benign	0.01
R1834:Unc80	UTSW	1	66,639,248 (GRCm38)	missense	possibly damaging	0.53
R1854:Unc80	UTSW	1	66,631,414 (GRCm38)	missense	possibly damaging	0.93
R1871:Unc80	UTSW	1	66,510,717 (GRCm38)	missense	possibly damaging	0.77
R1878:Unc80	UTSW	1	66,509,402 (GRCm38)	missense	probably damaging	0.96
R1883:Unc80	UTSW	1	66,525,770 (GRCm38)	missense	possibly damaging	0.89
R1912:Unc80	UTSW	1	66,510,625 (GRCm38)	missense	probably damaging	1.00
R1990:Unc80	UTSW	1	66,692,549 (GRCm38)	missense	probably damaging	0.97
R2007:Unc80	UTSW	1	66,503,776 (GRCm38)	missense	probably damaging	1.00
R2035:Unc80	UTSW	1	66,606,593 (GRCm38)	missense	probably damaging	0.98
R2056:Unc80	UTSW	1	66,640,552 (GRCm38)	missense	possibly damaging	0.72
R2060:Unc80	UTSW	1	66,640,595 (GRCm38)	missense	possibly damaging	0.53
R2074:Unc80	UTSW	1	66,679,744 (GRCm38)	critical splice donor site	probably null
R2088:Unc80	UTSW	1	66,590,227 (GRCm38)	missense	possibly damaging	0.77
R2089:Unc80	UTSW	1	66,671,715 (GRCm38)	splice site	probably benign
R2091:Unc80	UTSW	1	66,671,715 (GRCm38)	splice site	probably benign
R2139:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R2169:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R2175:Unc80	UTSW	1	66,677,355 (GRCm38)	missense	probably damaging	1.00
R2248:Unc80	UTSW	1	66,623,206 (GRCm38)	splice site	probably benign
R2255:Unc80	UTSW	1	66,618,258 (GRCm38)	missense	possibly damaging	0.53
R2308:Unc80	UTSW	1	66,648,997 (GRCm38)	missense	possibly damaging	0.53
R2484:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R2507:Unc80	UTSW	1	66,612,107 (GRCm38)	missense	possibly damaging	0.53
R2512:Unc80	UTSW	1	66,671,608 (GRCm38)	missense	possibly damaging	0.70
R2878:Unc80	UTSW	1	66,671,576 (GRCm38)	critical splice acceptor site	probably benign
R3040:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3104:Unc80	UTSW	1	66,623,291 (GRCm38)	missense	probably benign	0.33
R3402:Unc80	UTSW	1	66,510,686 (GRCm38)	missense	probably damaging	0.97
R3403:Unc80	UTSW	1	66,510,686 (GRCm38)	missense	probably damaging	0.97
R3413:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3426:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3427:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3428:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3904:Unc80	UTSW	1	66,639,296 (GRCm38)	nonsense	probably null
R3916:Unc80	UTSW	1	66,677,495 (GRCm38)	missense	probably benign	0.11
R3950:Unc80	UTSW	1	66,622,570 (GRCm38)	missense	possibly damaging	0.53
R4642:Unc80	UTSW	1	66,671,714 (GRCm38)	splice site	probably null
R4646:Unc80	UTSW	1	66,669,235 (GRCm38)	missense	probably benign	0.03
R4655:Unc80	UTSW	1	66,671,662 (GRCm38)	missense	probably benign	0.18
R4662:Unc80	UTSW	1	66,646,436 (GRCm38)	missense	probably benign	0.01
R4720:Unc80	UTSW	1	66,510,792 (GRCm38)	missense	possibly damaging	0.92
R4736:Unc80	UTSW	1	66,649,672 (GRCm38)	critical splice acceptor site	probably null
R4795:Unc80	UTSW	1	66,527,941 (GRCm38)	missense	probably damaging	0.97
R4888:Unc80	UTSW	1	66,644,447 (GRCm38)	missense	probably damaging	0.98
R4917:Unc80	UTSW	1	66,646,550 (GRCm38)	missense	possibly damaging	0.86
R4918:Unc80	UTSW	1	66,646,550 (GRCm38)	missense	possibly damaging	0.86
R4983:Unc80	UTSW	1	66,674,732 (GRCm38)	splice site	probably null
R5051:Unc80	UTSW	1	66,509,477 (GRCm38)	missense	probably damaging	0.96
R5111:Unc80	UTSW	1	66,527,995 (GRCm38)	missense	possibly damaging	0.66
R5122:Unc80	UTSW	1	66,679,590 (GRCm38)	missense	possibly damaging	0.53
R5260:Unc80	UTSW	1	66,646,587 (GRCm38)	missense	possibly damaging	0.53
R5351:Unc80	UTSW	1	66,606,513 (GRCm38)	missense	possibly damaging	0.73
R5387:Unc80	UTSW	1	66,530,021 (GRCm38)	missense	possibly damaging	0.77
R5437:Unc80	UTSW	1	66,654,578 (GRCm38)	missense	possibly damaging	0.96
R5525:Unc80	UTSW	1	66,606,614 (GRCm38)	missense	possibly damaging	0.72
R5621:Unc80	UTSW	1	66,638,043 (GRCm38)	missense	possibly damaging	0.53
R5690:Unc80	UTSW	1	66,640,572 (GRCm38)	missense	probably benign	0.08
R5762:Unc80	UTSW	1	66,693,796 (GRCm38)	missense	possibly damaging	0.82
R5956:Unc80	UTSW	1	66,527,964 (GRCm38)	missense	probably damaging	0.97
R6005:Unc80	UTSW	1	66,627,257 (GRCm38)	missense	possibly damaging	0.53
R6025:Unc80	UTSW	1	66,695,568 (GRCm38)	missense	possibly damaging	0.90
R6033:Unc80	UTSW	1	66,473,260 (GRCm38)	missense	possibly damaging	0.92
R6117:Unc80	UTSW	1	66,675,067 (GRCm38)	missense	possibly damaging	0.72
R6156:Unc80	UTSW	1	66,612,250 (GRCm38)	missense	probably benign	0.01
R6157:Unc80	UTSW	1	66,654,029 (GRCm38)	nonsense	probably null
R6189:Unc80	UTSW	1	66,677,471 (GRCm38)	missense	probably benign	0.33
R6291:Unc80	UTSW	1	66,521,597 (GRCm38)	missense	possibly damaging	0.82
R6367:Unc80	UTSW	1	66,672,766 (GRCm38)	missense	probably benign	0.33
R6598:Unc80	UTSW	1	66,468,540 (GRCm38)	critical splice donor site	probably null
R6724:Unc80	UTSW	1	66,683,191 (GRCm38)	missense	possibly damaging	0.90
R6763:Unc80	UTSW	1	66,521,477 (GRCm38)	missense	probably benign	0.00
R6773:Unc80	UTSW	1	66,651,543 (GRCm38)	missense	probably benign	0.33
R6883:Unc80	UTSW	1	66,646,404 (GRCm38)	missense	probably benign	0.33
R6951:Unc80	UTSW	1	66,648,511 (GRCm38)	missense	possibly damaging	0.53
R6965:Unc80	UTSW	1	66,646,566 (GRCm38)	missense	probably benign	0.33
R6993:Unc80	UTSW	1	66,549,793 (GRCm38)	missense	possibly damaging	0.60
R7041:Unc80	UTSW	1	66,503,593 (GRCm38)	missense	probably benign	0.00
R7050:Unc80	UTSW	1	66,550,908 (GRCm38)	splice site	probably null
R7067:Unc80	UTSW	1	66,646,572 (GRCm38)	missense	possibly damaging	0.86
R7080:Unc80	UTSW	1	66,646,521 (GRCm38)	missense	possibly damaging	0.53
R7193:Unc80	UTSW	1	66,549,784 (GRCm38)	missense	possibly damaging	0.60
R7197:Unc80	UTSW	1	66,521,566 (GRCm38)	nonsense	probably null
R7278:Unc80	UTSW	1	66,552,209 (GRCm38)	missense	possibly damaging	0.82
R7290:Unc80	UTSW	1	66,601,197 (GRCm38)	missense	probably damaging	0.97
R7391:Unc80	UTSW	1	66,695,528 (GRCm38)	missense	probably benign	0.18
R7401:Unc80	UTSW	1	66,646,415 (GRCm38)	missense	possibly damaging	0.96
R7470:Unc80	UTSW	1	66,622,462 (GRCm38)	missense	probably benign	0.02
R7573:Unc80	UTSW	1	66,521,537 (GRCm38)	missense	probably damaging	1.00
R7637:Unc80	UTSW	1	66,672,684 (GRCm38)	missense	possibly damaging	0.86
R7678:Unc80	UTSW	1	66,649,722 (GRCm38)	missense	probably benign	0.33
R7697:Unc80	UTSW	1	66,637,945 (GRCm38)	missense	possibly damaging	0.93
R7746:Unc80	UTSW	1	66,677,385 (GRCm38)	missense	probably benign	0.33
R7768:Unc80	UTSW	1	66,510,595 (GRCm38)	missense	possibly damaging	0.56
R7796:Unc80	UTSW	1	66,503,714 (GRCm38)	missense	probably benign
R7855:Unc80	UTSW	1	66,483,349 (GRCm38)	missense	possibly damaging	0.78
R7878:Unc80	UTSW	1	66,601,141 (GRCm38)	missense	possibly damaging	0.88
R7879:Unc80	UTSW	1	66,510,707 (GRCm38)	missense	probably benign	0.00
R8024:Unc80	UTSW	1	66,606,644 (GRCm38)	missense	possibly damaging	0.86
R8026:Unc80	UTSW	1	66,483,304 (GRCm38)	missense	possibly damaging	0.92
R8115:Unc80	UTSW	1	66,648,913 (GRCm38)	missense	probably benign	0.00
R8135:Unc80	UTSW	1	66,509,287 (GRCm38)	missense	possibly damaging	0.49
R8170:Unc80	UTSW	1	66,651,533 (GRCm38)	missense	probably benign	0.33
R8239:Unc80	UTSW	1	66,654,019 (GRCm38)	missense	probably benign
R8249:Unc80	UTSW	1	66,619,491 (GRCm38)	missense	probably benign	0.01
R8275:Unc80	UTSW	1	66,640,614 (GRCm38)	nonsense	probably null
R8288:Unc80	UTSW	1	66,473,350 (GRCm38)	missense	probably benign	0.07
R8341:Unc80	UTSW	1	66,649,033 (GRCm38)	missense	possibly damaging	0.73
R8356:Unc80	UTSW	1	66,641,629 (GRCm38)	missense	possibly damaging	0.85
R8433:Unc80	UTSW	1	66,638,028 (GRCm38)	nonsense	probably null
R8456:Unc80	UTSW	1	66,641,629 (GRCm38)	missense	possibly damaging	0.85
R8464:Unc80	UTSW	1	66,473,264 (GRCm38)	missense	probably damaging	1.00
R8483:Unc80	UTSW	1	66,693,710 (GRCm38)	missense	possibly damaging	0.83
R8509:Unc80	UTSW	1	66,641,629 (GRCm38)	missense	possibly damaging	0.85
R8686:Unc80	UTSW	1	66,612,268 (GRCm38)	missense	possibly damaging	0.53
R8701:Unc80	UTSW	1	66,638,032 (GRCm38)	missense	possibly damaging	0.85
R8729:Unc80	UTSW	1	66,608,490 (GRCm38)	missense	probably benign	0.01
R8755:Unc80	UTSW	1	66,612,131 (GRCm38)	missense	possibly damaging	0.53
R8771:Unc80	UTSW	1	66,646,395 (GRCm38)	missense	possibly damaging	0.85
R8866:Unc80	UTSW	1	66,590,229 (GRCm38)	missense	probably benign	0.05
R8877:Unc80	UTSW	1	66,527,985 (GRCm38)	missense	possibly damaging	0.89
R8942:Unc80	UTSW	1	66,473,309 (GRCm38)	missense	possibly damaging	0.94
R8976:Unc80	UTSW	1	66,472,010 (GRCm38)	missense	possibly damaging	0.87
R9063:Unc80	UTSW	1	66,606,657 (GRCm38)	critical splice donor site	probably null
R9095:Unc80	UTSW	1	66,506,753 (GRCm38)	missense	probably damaging	1.00
R9125:Unc80	UTSW	1	66,679,581 (GRCm38)	missense	probably benign	0.18
R9130:Unc80	UTSW	1	66,638,085 (GRCm38)	missense	possibly damaging	0.85
R9165:Unc80	UTSW	1	66,549,841 (GRCm38)	missense	probably null	0.95
R9220:Unc80	UTSW	1	66,507,375 (GRCm38)	missense	probably damaging	1.00
R9262:Unc80	UTSW	1	66,555,252 (GRCm38)	intron	probably benign
R9334:Unc80	UTSW	1	66,649,760 (GRCm38)	missense	possibly damaging	0.73
R9374:Unc80	UTSW	1	66,590,301 (GRCm38)	missense	possibly damaging	0.95
R9387:Unc80	UTSW	1	66,549,938 (GRCm38)	critical splice donor site	probably null
R9415:Unc80	UTSW	1	66,510,905 (GRCm38)	missense
R9427:Unc80	UTSW	1	66,554,999 (GRCm38)	missense	probably damaging	1.00
R9436:Unc80	UTSW	1	66,693,805 (GRCm38)	critical splice donor site	probably null
R9454:Unc80	UTSW	1	66,695,590 (GRCm38)	missense	possibly damaging	0.53
R9522:Unc80	UTSW	1	66,638,062 (GRCm38)	missense	possibly damaging	0.73
R9539:Unc80	UTSW	1	66,570,004 (GRCm38)	critical splice donor site	probably null
R9552:Unc80	UTSW	1	66,678,123 (GRCm38)	missense	possibly damaging	0.85
R9667:Unc80	UTSW	1	66,612,128 (GRCm38)	missense	possibly damaging	0.86
R9720:Unc80	UTSW	1	66,644,326 (GRCm38)	missense	possibly damaging	0.53
R9749:Unc80	UTSW	1	66,505,020 (GRCm38)	missense	probably damaging	0.99
R9789:Unc80	UTSW	1	66,612,212 (GRCm38)	missense	possibly damaging	0.53
X0019:Unc80	UTSW	1	66,648,382 (GRCm38)	missense	probably benign	0.33
X0021:Unc80	UTSW	1	66,509,266 (GRCm38)	critical splice acceptor site	probably null
X0024:Unc80	UTSW	1	66,491,046 (GRCm38)	missense	probably benign	0.21
X0062:Unc80	UTSW	1	66,623,259 (GRCm38)	missense	probably benign	0.02
X0066:Unc80	UTSW	1	66,530,757 (GRCm38)	missense	possibly damaging	0.77
Y4335:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
Y4336:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
Y4338:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
Z1088:Unc80	UTSW	1	66,646,451 (GRCm38)	missense	possibly damaging	0.85
Z1176:Unc80	UTSW	1	66,694,409 (GRCm38)	missense	probably benign
Z1177:Unc80	UTSW	1	66,695,339 (GRCm38)	missense	probably benign	0.03
Z1177:Unc80	UTSW	1	66,646,398 (GRCm38)	missense	possibly damaging	0.72

Predicted Primers

PCR Primer
(F):5'- TGTTCTGCAGAGATCTTAGCC -3'
(R):5'- TCTTCCTCATCATGGGAGCTG -3'

Sequencing Primer
(F):5'- TTCTGCAGAGATCTTAGCCAGGAAG -3'
(R):5'- CTCATCATGGGAGCTGCTTCG -3'

Posted On

2017-08-16