Mutagenetix > Incidental Mutation

Incidental Mutation 'R6035:Vmn1r85'

486504

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r85

Ensembl Gene

ENSMUSG00000070817

Gene Name

vomeronasal 1 receptor 85

Synonyms

V1rj3

MMRRC Submission

044207-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R6035 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

12818216-12819142 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 12818854 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 97 (S97P)

Ref Sequence

ENSEMBL: ENSMUSP00000148178 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094829] [ENSMUST00000209822]

AlphaFold

Q8VIB8

Predicted Effect

probably damaging
Transcript: ENSMUST00000094829
AA Change: S97P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000092424
Gene: ENSMUSG00000070817
AA Change: S97P

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	296	8.4e-18	PFAM
Pfam:V1R	24	298	2.5e-36	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000209822
AA Change: S97P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.6%

Validation Efficiency

84% (62/74)

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	G	T	6: 121,615,353 (GRCm39)	G76W	probably damaging	Het
Abca17	A	T	17: 24,500,219 (GRCm39)	F1324Y	possibly damaging	Het
Abca8b	A	T	11: 109,862,686 (GRCm39)		probably null	Het
Abcc12	A	G	8: 87,244,033 (GRCm39)	M1040T	probably damaging	Het
Abtb1	A	G	6: 88,818,788 (GRCm39)	F7L	probably damaging	Het
Adcy9	T	C	16: 4,122,377 (GRCm39)	T558A	probably benign	Het
Adgrb1	A	T	15: 74,412,292 (GRCm39)	T424S	possibly damaging	Het
Afg3l2	G	T	18: 67,554,329 (GRCm39)	L458M	probably damaging	Het
Ankrd31	C	A	13: 96,968,721 (GRCm39)	P786Q	probably benign	Het
Arhgap39	G	T	15: 76,621,424 (GRCm39)	Y392*	probably null	Het
Ash1l	T	C	3: 88,892,326 (GRCm39)	Y1402H	probably damaging	Het
Carmil2	G	T	8: 106,419,195 (GRCm39)	W749L	probably benign	Het
Ccar1	A	G	10: 62,587,564 (GRCm39)	Y867H	unknown	Het
Cdh13	A	G	8: 119,232,437 (GRCm39)	D47G	probably benign	Het
Chst9	T	A	18: 15,585,910 (GRCm39)	T218S	probably benign	Het
Clec2i	G	A	6: 128,870,587 (GRCm39)	V67I	probably benign	Het
Cox7a2	T	A	9: 79,667,028 (GRCm39)		probably benign	Het
Cplx3	A	G	9: 57,519,030 (GRCm39)		probably null	Het
Cpz	A	G	5: 35,674,929 (GRCm39)	C107R	probably damaging	Het
Dapk1	T	A	13: 60,909,013 (GRCm39)	C1209S	possibly damaging	Het
Ddx41	T	C	13: 55,681,781 (GRCm39)	M307V	probably benign	Het
Defa24	A	G	8: 22,224,565 (GRCm39)	I5V	probably benign	Het
Dgcr8	A	T	16: 18,076,178 (GRCm39)	N2K	probably damaging	Het
Ebf2	A	G	14: 67,476,423 (GRCm39)	D131G	probably damaging	Het
Fam149b	C	T	14: 20,427,985 (GRCm39)	R424C	probably damaging	Het
Fbln2	G	A	6: 91,240,335 (GRCm39)	V714M	probably damaging	Het
Fgf5	T	C	5: 98,423,385 (GRCm39)	Y257H	probably damaging	Het
Fmo3	A	C	1: 162,791,605 (GRCm39)	V224G	probably damaging	Het
Gigyf2	T	C	1: 87,338,450 (GRCm39)	I394T	possibly damaging	Het
Glmn	T	A	5: 107,741,746 (GRCm39)		probably null	Het
Greb1l	T	C	18: 10,501,025 (GRCm39)	I385T	possibly damaging	Het
Grhl1	C	A	12: 24,658,449 (GRCm39)	Q365K	probably benign	Het
Gsdme	G	A	6: 50,206,306 (GRCm39)	T179M	probably damaging	Het
Gtf2a1l	A	G	17: 89,018,962 (GRCm39)	T349A	probably benign	Het
Hax1	GTCATCATCATCATCATC	GTCATCATCATCATCATCATC	3: 89,905,247 (GRCm39)		probably benign	Het
Il5ra	G	A	6: 106,718,226 (GRCm39)	T76I	probably damaging	Het
Itga8	T	C	2: 12,196,525 (GRCm39)	T631A	probably benign	Het
Kcnh6	G	A	11: 105,909,978 (GRCm39)		probably null	Het
Krt26	C	T	11: 99,224,415 (GRCm39)	E368K	probably benign	Het
Lhx9	T	C	1: 138,766,281 (GRCm39)	D169G	possibly damaging	Het
Lmod3	A	G	6: 97,224,234 (GRCm39)	L529P	probably damaging	Het
Nup155	A	G	15: 8,173,577 (GRCm39)	T891A	probably benign	Het
Or11g24	A	G	14: 50,661,984 (GRCm39)	T3A	probably benign	Het
Or1e1	T	C	11: 73,244,582 (GRCm39)	M1T	probably null	Het
Or1j13	T	A	2: 36,369,996 (GRCm39)	I49F	probably damaging	Het
Or1p4-ps1	T	C	11: 74,208,285 (GRCm39)	*145R	probably null	Het
Or8b36	ATTGCTGTTT	ATTGCTGTTTGCTGTTT	9: 37,937,836 (GRCm39)		probably null	Het
Papln	G	C	12: 83,821,454 (GRCm39)	G262A	probably damaging	Het
Pdcd1lg2	G	A	19: 29,423,435 (GRCm39)	V160I	probably benign	Het
Pde8b	A	G	13: 95,164,105 (GRCm39)		probably benign	Het
Ppme1	G	A	7: 100,004,002 (GRCm39)	R68*	probably null	Het
Prob1	T	C	18: 35,787,835 (GRCm39)	S140G	probably benign	Het
Ptprn2	A	T	12: 117,219,215 (GRCm39)	N949Y	probably damaging	Het
Qser1	C	A	2: 104,617,468 (GRCm39)	D1115Y	probably damaging	Het
Rad54l	G	T	4: 115,954,666 (GRCm39)	D674E	probably damaging	Het
Ripk4	T	A	16: 97,545,387 (GRCm39)	D420V	probably damaging	Het
Ros1	G	T	10: 51,954,067 (GRCm39)	S1857R	probably benign	Het
Rsf1	A	G	7: 97,311,316 (GRCm39)	E682G	probably benign	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Homo
Samd4	G	A	14: 47,325,329 (GRCm39)	R515H	probably damaging	Het
Selp	T	A	1: 163,969,079 (GRCm39)	W560R	probably benign	Het
Shc3	A	T	13: 51,615,468 (GRCm39)	L163Q	probably damaging	Het
Shh	G	A	5: 28,666,397 (GRCm39)	A163V	probably damaging	Het
Slc17a8	T	C	10: 89,427,937 (GRCm39)	R113G	possibly damaging	Het
Slc5a6	C	A	5: 31,206,168 (GRCm39)		probably benign	Het
Smarcd2	A	G	11: 106,157,715 (GRCm39)		probably null	Het
Sytl3	A	G	17: 6,995,664 (GRCm39)	D148G	probably damaging	Het
Tnks	G	T	8: 35,385,615 (GRCm39)	H297Q	possibly damaging	Het
Trbv21	A	T	6: 41,179,568 (GRCm39)		probably benign	Het
Ube3c	T	C	5: 29,806,161 (GRCm39)	F268L	probably benign	Het
Ugt2b5	T	C	5: 87,287,541 (GRCm39)	I209V	probably benign	Het
Usp1	A	G	4: 98,818,082 (GRCm39)	N140S	probably damaging	Het
Vcam1	T	C	3: 115,919,606 (GRCm39)	Y226C	probably damaging	Het
Vmn1r129	T	A	7: 21,094,534 (GRCm39)	Q228L	probably damaging	Het
Vmn1r209	T	A	13: 22,990,202 (GRCm39)	N163Y	probably benign	Het
Vmn2r30	C	T	7: 7,337,350 (GRCm39)	M95I	probably benign	Het
Vmn2r74	G	A	7: 85,601,098 (GRCm39)	R847C	probably damaging	Het
Wdr70	G	A	15: 7,916,830 (GRCm39)	T529I	possibly damaging	Het
Zfp532	T	G	18: 65,757,005 (GRCm39)	S313A	possibly damaging	Het
Zhx3	A	T	2: 160,621,463 (GRCm39)	N901K	probably benign	Het

Other mutations in Vmn1r85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01099:Vmn1r85	APN	7	12,818,461 (GRCm39)	nonsense	probably null
IGL01520:Vmn1r85	APN	7	12,819,081 (GRCm39)	missense	probably damaging	1.00
IGL01803:Vmn1r85	APN	7	12,818,496 (GRCm39)	missense	probably damaging	0.98
IGL02285:Vmn1r85	APN	7	12,818,711 (GRCm39)	missense	probably damaging	1.00
IGL02852:Vmn1r85	APN	7	12,819,010 (GRCm39)	missense	possibly damaging	0.70
IGL03012:Vmn1r85	APN	7	12,818,692 (GRCm39)	missense	probably benign	0.01
R0391:Vmn1r85	UTSW	7	12,818,515 (GRCm39)	missense	probably benign	0.01
R0655:Vmn1r85	UTSW	7	12,818,650 (GRCm39)	missense	probably damaging	1.00
R1142:Vmn1r85	UTSW	7	12,818,481 (GRCm39)	missense	probably benign	0.01
R1452:Vmn1r85	UTSW	7	12,818,808 (GRCm39)	missense	probably damaging	1.00
R1942:Vmn1r85	UTSW	7	12,818,668 (GRCm39)	missense	possibly damaging	0.83
R3760:Vmn1r85	UTSW	7	12,818,932 (GRCm39)	missense	probably damaging	0.96
R4783:Vmn1r85	UTSW	7	12,818,788 (GRCm39)	missense	probably damaging	1.00
R4785:Vmn1r85	UTSW	7	12,818,788 (GRCm39)	missense	probably damaging	1.00
R5373:Vmn1r85	UTSW	7	12,818,255 (GRCm39)	nonsense	probably null
R6021:Vmn1r85	UTSW	7	12,818,616 (GRCm39)	missense	probably benign	0.00
R6035:Vmn1r85	UTSW	7	12,818,854 (GRCm39)	missense	probably damaging	1.00
R6834:Vmn1r85	UTSW	7	12,818,571 (GRCm39)	missense	probably damaging	1.00
R7340:Vmn1r85	UTSW	7	12,819,073 (GRCm39)	missense	probably damaging	1.00
R7346:Vmn1r85	UTSW	7	12,818,964 (GRCm39)	missense	probably damaging	0.99
R7836:Vmn1r85	UTSW	7	12,818,698 (GRCm39)	missense	probably benign	0.06
R7896:Vmn1r85	UTSW	7	12,818,448 (GRCm39)	missense	probably benign	0.22
R9090:Vmn1r85	UTSW	7	12,818,942 (GRCm39)	nonsense	probably null
R9271:Vmn1r85	UTSW	7	12,818,942 (GRCm39)	nonsense	probably null
R9354:Vmn1r85	UTSW	7	12,818,725 (GRCm39)	missense	probably damaging	1.00
R9713:Vmn1r85	UTSW	7	12,818,458 (GRCm39)	missense	probably damaging	1.00
R9779:Vmn1r85	UTSW	7	12,818,308 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GATTGGGGTACTCAACTGGAC -3'
(R):5'- GCTCGCTTTAACAGACAGATCG -3'

Sequencing Primer
(F):5'- GGTACTCAACTGGACTACTTTCACAG -3'
(R):5'- CAGACAGATCGATCCCATTTTCATG -3'

Posted On

2017-08-16