Mutagenetix > Incidental Mutation

Incidental Mutation 'R6036:Ifna15'

486564

Institutional Source

Beutler Lab

Gene Symbol

Ifna15

Ensembl Gene

ENSMUSG00000096011

Gene Name

interferon alpha 15

Synonyms

Ifnaa, Gm12597

MMRRC Submission

043257-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R6036 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

88557673-88558245 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 88558073 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 58 (D58A)

Ref Sequence

ENSEMBL: ENSMUSP00000099873 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102809]

AlphaFold

Q61718

Predicted Effect

possibly damaging
Transcript: ENSMUST00000102809
AA Change: D58A

PolyPhen 2 Score 0.655 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000099873
Gene: ENSMUSG00000096011
AA Change: D58A

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	2.97e-68	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abi3	T	C	11: 95,832,858		probably benign	Het
Ago1	C	T	4: 126,443,228	R228H	probably damaging	Het
Alpk3	G	A	7: 81,093,257	V941M	probably benign	Het
Ano4	A	G	10: 88,982,265	W588R	possibly damaging	Het
Atp6v1a	A	G	16: 44,098,831	Y464H	probably benign	Het
Barx2	A	T	9: 31,913,008	D28E	probably damaging	Het
Cabp5	A	T	7: 13,401,335	M67L	probably damaging	Het
Col10a1	A	G	10: 34,395,282	T417A	probably benign	Het
Dnah2	C	T	11: 69,458,920	R2399Q	probably benign	Het
Ehmt2	C	T	17: 34,899,091	R40*	probably null	Het
Eif4enif1	T	C	11: 3,239,420	S227P	probably damaging	Het
Erv3	G	A	2: 131,856,005	H145Y	possibly damaging	Het
Exoc5	T	C	14: 49,014,322	T591A	possibly damaging	Het
F5	A	T	1: 164,184,996	E493V	probably damaging	Het
Gm8444	G	T	15: 81,843,593		probably benign	Het
Gria4	T	A	9: 4,537,646	I221L	probably benign	Het
Gtf2h3	C	T	5: 124,584,297	T121I	probably benign	Het
Hc	T	C	2: 35,039,684	T249A	probably benign	Het
Herc2	A	G	7: 56,068,053	T48A	probably benign	Het
Hist1h2bl	A	T	13: 21,715,978	S56T	probably damaging	Het
Hp	A	G	8: 109,576,774		probably null	Het
Kcnj1	A	T	9: 32,397,125	M262L	probably benign	Het
Krt83	A	C	15: 101,487,531	I320S	possibly damaging	Het
Megf10	A	G	18: 57,242,727	N242D	probably damaging	Het
Nup155	A	G	15: 8,128,411	T451A	probably benign	Het
Olfr1277	C	T	2: 111,269,612	G252R	probably damaging	Het
Olfr1288	T	C	2: 111,478,988	L68P	probably damaging	Het
Olfr559	T	A	7: 102,724,485	I2F	probably benign	Het
Olfr701	A	T	7: 106,818,460	I126F	probably damaging	Het
Olfr930	A	G	9: 38,930,920	I250V	probably damaging	Het
Pdzd8	G	A	19: 59,305,209	P403S	probably damaging	Het
Piezo2	G	A	18: 63,114,948	Q494*	probably null	Het
Pik3c2b	T	C	1: 133,090,713	F966S	possibly damaging	Het
Plag1	T	C	4: 3,904,618	E191G	possibly damaging	Het
Pou4f2	A	T	8: 78,435,474	S167T	probably damaging	Het
Rsf1	G	GACGGCGGCT	7: 97,579,909		probably benign	Het
Scd4	G	A	19: 44,344,792	D319N	probably damaging	Het
Senp2	A	T	16: 22,028,558	R279*	probably null	Het
Sh3rf3	G	A	10: 58,813,984	G137D	probably benign	Het
Simc1	C	T	13: 54,524,621	P261S	probably benign	Het
Skint8	C	A	4: 111,950,193	L359M	probably damaging	Het
Slc26a1	T	A	5: 108,673,570	D151V	probably damaging	Het
Snx29	A	G	16: 11,738,437		probably null	Het
Stard9	C	T	2: 120,700,075	A2271V	probably benign	Het
Stat6	A	G	10: 127,655,444	N485D	possibly damaging	Het
Tpcn2	T	C	7: 145,268,869	T280A	possibly damaging	Het
Ttc23	A	G	7: 67,711,366	I378V	possibly damaging	Het
Ttc29	A	G	8: 78,325,576	D362G	probably benign	Het
Ttll7	A	G	3: 146,940,162	I592V	probably benign	Het
Ugt3a1	A	T	15: 9,306,086	H107L	probably benign	Het
Vmn1r70	A	G	7: 10,633,903	Q87R	probably damaging	Het
Vmn2r-ps129	T	C	17: 22,995,172		noncoding transcript	Het
Wdfy4	G	T	14: 33,146,990	S360R	probably damaging	Het
Zfp780b	A	G	7: 27,963,568	Y521H	probably damaging	Het

Other mutations in Ifna15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01575:Ifna15	APN	4	88557807	missense	probably damaging	1.00
IGL01757:Ifna15	APN	4	88558085	missense	possibly damaging	0.79
IGL01824:Ifna15	APN	4	88557783	missense	probably benign	0.00
R3853:Ifna15	UTSW	4	88557809	missense	probably damaging	1.00
R4356:Ifna15	UTSW	4	88557842	missense	probably benign	0.09
R4357:Ifna15	UTSW	4	88557842	missense	probably benign	0.09
R4358:Ifna15	UTSW	4	88557842	missense	probably benign	0.09
R4359:Ifna15	UTSW	4	88557842	missense	probably benign	0.09
R5038:Ifna15	UTSW	4	88558029	missense	probably benign	0.03
R5372:Ifna15	UTSW	4	88558101	missense	probably damaging	0.97
R6036:Ifna15	UTSW	4	88558073	missense	possibly damaging	0.65
R7347:Ifna15	UTSW	4	88557983	missense	probably damaging	1.00
R8097:Ifna15	UTSW	4	88557701	missense	probably benign	0.01
R8132:Ifna15	UTSW	4	88557683	missense	possibly damaging	0.60
R8824:Ifna15	UTSW	4	88557761	missense	probably damaging	1.00
R9349:Ifna15	UTSW	4	88558046	missense	probably benign	0.10

Predicted Primers

PCR Primer
(F):5'- GCATCACACAGGCTTTGAGG -3'
(R):5'- CAGAGGACCAGCAACATCTG -3'

Sequencing Primer
(F):5'- CATCACACAGGCTTTGAGGTCATTG -3'
(R):5'- AGATCCACAATGGCTAGGCTCTG -3'

Posted On

2017-08-16