Mutagenetix > Incidental Mutation

Incidental Mutation 'R5268:Olfr304'

486793

Institutional Source

Beutler Lab

Gene Symbol

Olfr304

Ensembl Gene

ENSMUSG00000062426

Gene Name

olfactory receptor 304

Synonyms

GA_x6K02T2NHDJ-9721756-9722757, MOR219-3P

MMRRC Submission

042860-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R5268 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86385657-86386658 bp(-) (GRCm38)

Type of Mutation

makesense

DNA Base Change (assembly)

A to G at 86385659 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Glutamine at position 334 (*334Q)

Ref Sequence

ENSEMBL: ENSMUSP00000076449 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077210]

AlphaFold

Q7TS03

Predicted Effect

probably null
Transcript: ENSMUST00000077210
AA Change: *334Q

SMART Domains

Protein: ENSMUSP00000076449
Gene: ENSMUSG00000062426
AA Change: *334Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	8.9e-38	PFAM
Pfam:7tm_1	39	288	1.1e-21	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810032O08Rik	G	T	11: 116,671,756	M18I	probably benign	Het
Adamtsl4	C	A	3: 95,680,163	R701L	probably damaging	Het
Adgrf5	T	C	17: 43,450,999	V1195A	probably damaging	Het
Agpat5	A	G	8: 18,881,862	T333A	possibly damaging	Het
Aspm	G	T	1: 139,464,295	G795C	probably damaging	Het
Car4	C	A	11: 84,965,800	Q305K	probably benign	Het
Catsperg1	T	C	7: 29,195,247	N612S	probably benign	Het
Cdc5l	G	A	17: 45,415,585	R354W	probably damaging	Het
Cdh9	G	A	15: 16,851,013	V623I	probably benign	Het
Cfap65	A	G	1: 74,924,902	V629A	probably benign	Het
Chd1	G	A	17: 15,735,743	V535I	probably damaging	Het
Col12a1	A	G	9: 79,678,047	V1316A	probably damaging	Het
Col6a3	A	T	1: 90,785,243	D1721E	unknown	Het
Cop1	T	A	1: 159,327,164	F647L	probably damaging	Het
Corin	A	C	5: 72,343,019	S421A	probably damaging	Het
Crb2	T	C	2: 37,790,821	V587A	probably damaging	Het
Ctc1	T	A	11: 69,029,810	V646E	possibly damaging	Het
Cyp2a12	A	T	7: 27,031,218	M203L	probably benign	Het
Dcdc2c	T	A	12: 28,516,657	K336M	possibly damaging	Het
Ddx39	G	A	8: 83,722,321	R275H	probably benign	Het
Des	A	G	1: 75,362,928	N310S	possibly damaging	Het
Dlk1	C	A	12: 109,459,838	S212R	probably benign	Het
Dnajc5b	A	G	3: 19,579,060	E149G	probably benign	Het
Dpep1	A	T	8: 123,194,089	I3F	probably benign	Het
Eml6	T	A	11: 29,803,108	R934S	probably benign	Het
Ercc6l2	T	C	13: 63,869,111	L676P	possibly damaging	Het
Fkbpl	G	A	17: 34,645,329	A24T	probably benign	Het
Frem2	T	A	3: 53,653,154	I1311F	probably damaging	Het
Gldc	T	A	19: 30,145,725	M257L	probably damaging	Het
Gm5538	A	G	3: 59,752,023	E299G	probably damaging	Het
Gm8674	T	G	13: 49,901,354		noncoding transcript	Het
Gnai3	C	T	3: 108,123,541		probably null	Het
Gtf3a	A	G	5: 146,955,155	D300G	probably damaging	Het
Haus3	A	T	5: 34,166,105	V387D	probably damaging	Het
Hpf1	T	A	8: 60,893,734	F28I	possibly damaging	Het
Iars	A	G	13: 49,690,491	D147G	probably damaging	Het
Ibtk	A	T	9: 85,743,690	D2E	probably benign	Het
Ift140	T	A	17: 25,020,627	I86K	possibly damaging	Het
Igf2bp2	T	A	16: 22,079,491	I235F	probably damaging	Het
Ighv3-8	G	T	12: 114,322,632	A30E	probably benign	Het
Kcnq5	T	A	1: 21,505,725	T252S	probably damaging	Het
Lrrc4b	A	T	7: 44,461,363	N220Y	probably damaging	Het
Ltbr	G	A	6: 125,312,794	R146W	probably damaging	Het
Mamdc4	T	C	2: 25,564,690	T1037A	possibly damaging	Het
Mbd5	A	T	2: 49,272,094	I863F	possibly damaging	Het
Muc4	T	A	16: 32,751,666	S515T	possibly damaging	Het
Mylk4	T	A	13: 32,708,881		probably null	Het
Neu4	G	A	1: 94,024,947	R346H	probably benign	Het
Nxpe5	T	A	5: 138,239,938	Y253*	probably null	Het
Olfml2b	A	T	1: 170,649,761	E155V	probably damaging	Het
Olfr160	T	A	9: 37,712,004	I92F	probably damaging	Het
Olfr2	T	A	7: 107,000,904	N319Y	probably benign	Het
Pamr1	G	A	2: 102,586,684	A114T	probably damaging	Het
Pdzd2	T	A	15: 12,592,177	N127I	probably damaging	Het
Pglyrp4	C	T	3: 90,726,964	L5F	probably damaging	Het
Plce1	T	A	19: 38,758,835	S1667T	possibly damaging	Het
Qser1	A	T	2: 104,787,431	V1012E	possibly damaging	Het
R3hdm4	C	T	10: 79,912,458	E162K	possibly damaging	Het
Rcn3	A	T	7: 45,086,779	D168E	probably damaging	Het
Rdh1	T	G	10: 127,759,963	V9G	possibly damaging	Het
Rin2	A	G	2: 145,844,760	T70A	probably benign	Het
Rnf207	T	C	4: 152,313,889	D276G	probably damaging	Het
Rtel1	C	T	2: 181,340,561	S368L	probably benign	Het
Slc15a5	C	A	6: 138,079,753	C55F	probably damaging	Het
Slc36a4	T	C	9: 15,726,916	F213L	possibly damaging	Het
Spata25	C	T	2: 164,828,034	G19D	probably damaging	Het
St5	A	G	7: 109,557,312	L77P	probably benign	Het
Tas2r102	A	T	6: 132,762,397	L89F	probably damaging	Het
Tbc1d1	A	G	5: 64,324,567	K704E	probably damaging	Het
Tert	T	G	13: 73,627,354	S75A	probably damaging	Het
Tiparp	T	A	3: 65,547,565	L195Q	possibly damaging	Het
Tmem205	A	C	9: 21,926,084	Y57*	probably null	Het
Tomm22	A	G	15: 79,671,227	R5G	probably benign	Het
Trav10	A	T	14: 53,506,058	S39C	probably benign	Het
Trav6-2	T	A	14: 52,667,748	N75K	probably benign	Het
Trio	T	C	15: 27,748,286	S2427G	probably benign	Het
Ttc41	C	A	10: 86,744,478	H763N	possibly damaging	Het
Usp29	A	T	7: 6,961,584	N142I	probably damaging	Het
Vmn1r204	G	T	13: 22,556,742	W181L	probably damaging	Het
Vmn2r76	G	A	7: 86,226,059	T570M	probably damaging	Het
Wdr90	A	G	17: 25,850,845	L1173P	probably damaging	Het
Wnt9a	T	A	11: 59,328,570	C141S	probably damaging	Het
Zfp462	A	T	4: 55,012,299	I1422L	probably benign	Het

Other mutations in Olfr304

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01391:Olfr304	APN	7	86386000	missense	possibly damaging	0.95
IGL02152:Olfr304	APN	7	86386043	missense	probably benign	0.00
IGL02540:Olfr304	APN	7	86386178	missense	possibly damaging	0.86
IGL03108:Olfr304	APN	7	86385721	missense	possibly damaging	0.95
IGL03374:Olfr304	APN	7	86386366	missense	probably damaging	1.00
R0040:Olfr304	UTSW	7	86386507	missense	probably benign	0.01
R0130:Olfr304	UTSW	7	86386306	missense	probably damaging	1.00
R0194:Olfr304	UTSW	7	86386374	nonsense	probably null
R0267:Olfr304	UTSW	7	86386267	missense	possibly damaging	0.64
R1026:Olfr304	UTSW	7	86386259	missense	probably damaging	0.98
R1865:Olfr304	UTSW	7	86386561	missense	probably damaging	1.00
R2090:Olfr304	UTSW	7	86386081	missense	probably benign	0.01
R3607:Olfr304	UTSW	7	86385695	missense	probably benign
R3861:Olfr304	UTSW	7	86386123	missense	possibly damaging	0.60
R3909:Olfr304	UTSW	7	86385974	missense	probably benign	0.05
R4113:Olfr304	UTSW	7	86386525	missense	possibly damaging	0.83
R5649:Olfr304	UTSW	7	86386313	missense	probably damaging	1.00
R6343:Olfr304	UTSW	7	86385851	nonsense	probably null
R7716:Olfr304	UTSW	7	86386054	missense	probably benign	0.22
R8118:Olfr304	UTSW	7	86385768	nonsense	probably null
R9047:Olfr304	UTSW	7	86386040	missense	probably benign	0.05
R9503:Olfr304	UTSW	7	86386020	missense	probably benign	0.00
R9528:Olfr304	UTSW	7	86385851	nonsense	probably null
X0054:Olfr304	UTSW	7	86385730	missense	probably benign	0.00
X0063:Olfr304	UTSW	7	86386492	missense	probably damaging	1.00
Z1186:Olfr304	UTSW	7	86386279	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGTATGTGGTAGCTTCTTGCC -3'
(R):5'- TGTATACTGTGCTGCCTCCAG -3'

Sequencing Primer
(F):5'- ATGTGGTAGCTTCTTGCCTATTC -3'
(R):5'- CCAGCACTTAATCCTGTGATCTAC -3'

Posted On

2017-08-17