Mutagenetix > Incidental Mutation

Incidental Mutation 'R6133:Pcdh15'

487243

Institutional Source

Beutler Lab

Gene Symbol

Pcdh15

Ensembl Gene

ENSMUSG00000052613

Gene Name

protocadherin 15

Synonyms

Gm9815, nmf19, roda, Ush1f

MMRRC Submission

044280-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6133 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

72935174-74485569 bp(+) (GRCm39)

Type of Mutation

splice site (1596 bp from exon)

DNA Base Change (assembly)

A to T at 74481805 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000142238 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000124046] [ENSMUST00000125517] [ENSMUST00000131724] [ENSMUST00000144302] [ENSMUST00000146682] [ENSMUST00000149977] [ENSMUST00000195531] [ENSMUST00000191854] [ENSMUST00000177107] [ENSMUST00000193739] [ENSMUST00000152819] [ENSMUST00000191709] [ENSMUST00000193174] [ENSMUST00000152655] [ENSMUST00000155701] [ENSMUST00000151116]

AlphaFold

Q99PJ1

Predicted Effect

probably null
Transcript: ENSMUST00000124046

SMART Domains

Protein: ENSMUSP00000121130
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
CA	30	118	7.87e-9	SMART
CA	142	224	4.88e-14	SMART
CA	249	326	4.65e-20	SMART
CA	350	428	1.93e-26	SMART
CA	452	535	5.69e-15	SMART
CA	559	645	6.85e-9	SMART
CA	666	753	3.09e-16	SMART
CA	777	861	4.49e-4	SMART
transmembrane domain	986	1008	N/A	INTRINSIC
low complexity region	1029	1056	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000125517

SMART Domains

Protein: ENSMUSP00000115399
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	1.22e-1	SMART
CA	174	263	5.48e-8	SMART
CA	304	393	1.94e-8	SMART
low complexity region	430	468	N/A	INTRINSIC
low complexity region	521	584	N/A	INTRINSIC
low complexity region	610	641	N/A	INTRINSIC
low complexity region	657	685	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127928

Predicted Effect

probably null
Transcript: ENSMUST00000131724

SMART Domains

Protein: ENSMUSP00000122466
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	1.22e-1	SMART
CA	174	263	5.48e-8	SMART
CA	304	393	1.94e-8	SMART
CA	426	507	2.29e-10	SMART
CA	531	613	4.88e-14	SMART
CA	638	715	4.65e-20	SMART
CA	739	817	1.93e-26	SMART
CA	841	924	5.69e-15	SMART
CA	948	1034	6.85e-9	SMART
CA	1055	1142	3.09e-16	SMART
CA	1166	1250	4.49e-4	SMART
transmembrane domain	1375	1397	N/A	INTRINSIC
low complexity region	1418	1445	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000144302

SMART Domains

Protein: ENSMUSP00000122606
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	1.22e-1	SMART
CA	174	263	5.48e-8	SMART
low complexity region	313	326	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000146682
AA Change: R383S

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000134863
Gene: ENSMUSG00000052613
AA Change: R383S

Domain	Start	End	E-Value	Type
transmembrane domain	128	150	N/A	INTRINSIC
low complexity region	215	232	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000149977
AA Change: R1643S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000118833
Gene: ENSMUSG00000052613
AA Change: R1643S

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	1.22e-1	SMART
CA	174	263	5.48e-8	SMART
CA	304	393	1.94e-8	SMART
CA	426	507	2.29e-10	SMART
CA	531	613	4.88e-14	SMART
CA	638	715	4.65e-20	SMART
CA	739	817	1.93e-26	SMART
CA	841	924	5.69e-15	SMART
CA	948	1034	6.85e-9	SMART
CA	1055	1142	3.09e-16	SMART
CA	1166	1250	4.49e-4	SMART
transmembrane domain	1375	1397	N/A	INTRINSIC
low complexity region	1418	1445	N/A	INTRINSIC
low complexity region	1477	1494	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000195531
AA Change: R1682S

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000141920
Gene: ENSMUSG00000052613
AA Change: R1682S

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	6e-4	SMART
CA	174	263	2.8e-10	SMART
CA	304	393	9.4e-11	SMART
CA	426	507	1.2e-12	SMART
CA	531	613	2.3e-16	SMART
CA	638	715	2.3e-22	SMART
CA	739	817	9.3e-29	SMART
CA	841	924	2.8e-17	SMART
CA	948	1034	3.3e-11	SMART
CA	1055	1142	1.5e-18	SMART
CA	1166	1250	2.3e-6	SMART
transmembrane domain	1377	1399	N/A	INTRINSIC
low complexity region	1415	1442	N/A	INTRINSIC
low complexity region	1514	1531	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000191854
AA Change: R1645S

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000141973
Gene: ENSMUSG00000052613
AA Change: R1645S

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	1.22e-1	SMART
CA	174	263	5.48e-8	SMART
CA	304	393	1.94e-8	SMART
CA	426	507	2.29e-10	SMART
CA	531	613	4.88e-14	SMART
CA	638	715	4.65e-20	SMART
CA	739	817	1.93e-26	SMART
CA	841	924	5.69e-15	SMART
CA	948	1034	6.85e-9	SMART
CA	1055	1142	3.09e-16	SMART
CA	1166	1250	4.49e-4	SMART
transmembrane domain	1375	1397	N/A	INTRINSIC
low complexity region	1418	1445	N/A	INTRINSIC
low complexity region	1477	1494	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000177107
AA Change: R1650S

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000135501
Gene: ENSMUSG00000052613
AA Change: R1650S

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	69	150	1.22e-1	SMART
CA	179	268	5.48e-8	SMART
CA	309	398	1.94e-8	SMART
CA	431	512	2.29e-10	SMART
CA	536	618	4.88e-14	SMART
CA	643	720	4.65e-20	SMART
CA	744	822	1.93e-26	SMART
CA	846	929	5.69e-15	SMART
CA	953	1039	6.85e-9	SMART
CA	1060	1147	3.09e-16	SMART
CA	1171	1255	4.49e-4	SMART
transmembrane domain	1380	1402	N/A	INTRINSIC
low complexity region	1423	1450	N/A	INTRINSIC
low complexity region	1482	1499	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000193739
AA Change: R1645S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000142173
Gene: ENSMUSG00000052613
AA Change: R1645S

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	69	150	1.22e-1	SMART
CA	179	268	5.48e-8	SMART
CA	309	398	1.94e-8	SMART
CA	431	512	2.29e-10	SMART
CA	536	618	4.88e-14	SMART
CA	643	720	4.65e-20	SMART
CA	744	822	1.93e-26	SMART
CA	846	929	5.69e-15	SMART
CA	953	1039	6.85e-9	SMART
CA	1060	1147	3.09e-16	SMART
CA	1171	1255	4.49e-4	SMART
transmembrane domain	1382	1404	N/A	INTRINSIC
low complexity region	1420	1447	N/A	INTRINSIC
low complexity region	1477	1494	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000192370

Predicted Effect

probably null
Transcript: ENSMUST00000152819

SMART Domains

Protein: ENSMUSP00000123647
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	1.22e-1	SMART
CA	174	263	5.48e-8	SMART
Blast:CA	304	363	1e-33	BLAST
low complexity region	364	399	N/A	INTRINSIC
low complexity region	452	515	N/A	INTRINSIC
low complexity region	541	572	N/A	INTRINSIC
low complexity region	588	616	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000191709

SMART Domains

Protein: ENSMUSP00000142313
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	69	150	1.22e-1	SMART
CA	179	268	5.48e-8	SMART
CA	309	398	1.94e-8	SMART
CA	431	512	2.29e-10	SMART
CA	536	618	4.88e-14	SMART
CA	643	720	4.65e-20	SMART
CA	744	822	1.93e-26	SMART
CA	846	929	5.69e-15	SMART
CA	953	1039	6.85e-9	SMART
CA	1060	1147	3.09e-16	SMART
CA	1171	1255	4.49e-4	SMART
transmembrane domain	1380	1402	N/A	INTRINSIC
low complexity region	1423	1450	N/A	INTRINSIC
low complexity region	1480	1510	N/A	INTRINSIC
low complexity region	1512	1530	N/A	INTRINSIC
low complexity region	1583	1646	N/A	INTRINSIC
low complexity region	1672	1703	N/A	INTRINSIC
low complexity region	1719	1747	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000193174

SMART Domains

Protein: ENSMUSP00000142238
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	6e-4	SMART
CA	174	263	2.8e-10	SMART
CA	304	393	9.4e-11	SMART
CA	426	514	3.8e-11	SMART
CA	538	620	2.3e-16	SMART
CA	645	722	2.3e-22	SMART
CA	746	824	9.3e-29	SMART
CA	848	931	2.8e-17	SMART
CA	955	1041	3.3e-11	SMART
CA	1062	1149	1.5e-18	SMART
CA	1173	1257	2.3e-6	SMART
transmembrane domain	1382	1404	N/A	INTRINSIC
low complexity region	1425	1452	N/A	INTRINSIC
low complexity region	1482	1512	N/A	INTRINSIC
low complexity region	1514	1532	N/A	INTRINSIC
low complexity region	1585	1648	N/A	INTRINSIC
low complexity region	1674	1705	N/A	INTRINSIC
low complexity region	1721	1749	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000152655

SMART Domains

Protein: ENSMUSP00000118201
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	6e-4	SMART
CA	174	263	2.8e-10	SMART
CA	304	393	9.4e-11	SMART
CA	426	507	1.2e-12	SMART
CA	531	613	2.3e-16	SMART
CA	638	726	3.4e-6	SMART
low complexity region	783	846	N/A	INTRINSIC
low complexity region	872	903	N/A	INTRINSIC
low complexity region	919	947	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000155701

SMART Domains

Protein: ENSMUSP00000135495
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	64	145	1.22e-1	SMART
CA	174	263	5.48e-8	SMART
Blast:CA	304	330	2e-8	BLAST

Predicted Effect

probably null
Transcript: ENSMUST00000151116

SMART Domains

Protein: ENSMUSP00000119662
Gene: ENSMUSG00000052613

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
CA	69	150	1.22e-1	SMART
CA	179	268	5.48e-8	SMART
CA	309	398	1.94e-8	SMART
CA	431	519	7.87e-9	SMART
CA	543	625	4.88e-14	SMART
CA	650	727	4.65e-20	SMART
CA	751	829	1.93e-26	SMART
CA	853	936	5.69e-15	SMART
CA	960	1046	6.85e-9	SMART
CA	1067	1154	3.09e-16	SMART
CA	1178	1262	4.49e-4	SMART
transmembrane domain	1387	1409	N/A	INTRINSIC
low complexity region	1430	1457	N/A	INTRINSIC
low complexity region	1489	1519	N/A	INTRINSIC
low complexity region	1521	1539	N/A	INTRINSIC
low complexity region	1592	1655	N/A	INTRINSIC
low complexity region	1681	1712	N/A	INTRINSIC
low complexity region	1728	1756	N/A	INTRINSIC

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.2%

Validation Efficiency

100% (47/47)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]
PHENOTYPE: Homozygotes for severe mutations exhibit circling, head-tossing, hyperactivity, impaired swimming and profound deafness. Mice have defects in cochlea and degeneration of hair cells, spiral ganglion cells and saccular macula. Females are poor mothers. [provided by MGI curators]

Allele List at MGI

All alleles(11) : Gene trapped(2) Transgenic(1) Spontaneous(6) Chemically induced(2)

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy7	A	G	8: 89,052,067 (GRCm39)	T912A	possibly damaging	Het
Akap12	G	T	10: 4,305,178 (GRCm39)	G663C	probably benign	Het
Ankhd1	T	C	18: 36,758,179 (GRCm39)	S958P	possibly damaging	Het
Cmtm2a	T	C	8: 105,019,362 (GRCm39)	I76V	probably benign	Het
Cpxm2	G	A	7: 131,730,182 (GRCm39)	P146S	probably damaging	Het
Cubn	A	G	2: 13,313,429 (GRCm39)	V3047A	probably benign	Het
Dgkd	T	A	1: 87,865,962 (GRCm39)	V198E	possibly damaging	Het
Dnah3	A	T	7: 119,685,469 (GRCm39)	M181K	probably benign	Het
Dnah7a	T	C	1: 53,458,814 (GRCm39)	T3775A	probably benign	Het
Dsg2	A	G	18: 20,723,146 (GRCm39)	I391V	probably benign	Het
Ebi3	T	A	17: 56,261,311 (GRCm39)	V69E	probably benign	Het
Fn1	T	C	1: 71,636,886 (GRCm39)	T1998A	probably damaging	Het
Frmpd1	T	A	4: 45,284,915 (GRCm39)	H1245Q	probably benign	Het
Gm7145	T	A	1: 117,913,618 (GRCm39)	C167S	probably damaging	Het
Hydin	C	T	8: 111,327,908 (GRCm39)	T4805I	probably benign	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Kcnma1	C	T	14: 24,053,936 (GRCm39)	M21I	probably damaging	Het
Lrfn5	A	G	12: 61,890,574 (GRCm39)	D621G	probably benign	Het
Lrrc15	C	T	16: 30,093,054 (GRCm39)	G95D	probably benign	Het
Mex3d	A	G	10: 80,222,620 (GRCm39)	L212P	probably damaging	Het
Mmel1	C	T	4: 154,979,475 (GRCm39)	H728Y	probably damaging	Het
Naip1	A	G	13: 100,581,151 (GRCm39)	V32A	probably benign	Het
Nsl1	T	C	1: 190,803,403 (GRCm39)	L158P	probably damaging	Het
Or51q1c	A	T	7: 103,652,532 (GRCm39)	T17S	possibly damaging	Het
Or6c202	G	A	10: 128,996,752 (GRCm39)	L34F	possibly damaging	Het
Or8b36	ATTGCTGTTT	ATTGCTGTTTGCTGTTT	9: 37,937,836 (GRCm39)		probably null	Het
Pakap	C	T	4: 57,855,516 (GRCm39)	Q525*	probably null	Het
Pramel15	T	C	4: 144,104,347 (GRCm39)	R53G	possibly damaging	Het
Ptpn1	T	C	2: 167,809,716 (GRCm39)	V108A	possibly damaging	Het
Rad9b	T	C	5: 122,477,831 (GRCm39)	N182D	possibly damaging	Het
Rp1l1	C	T	14: 64,267,545 (GRCm39)	P1044S	probably damaging	Het
Scn2a	G	A	2: 65,573,448 (GRCm39)	V1433I	probably benign	Het
Ssrp1	T	G	2: 84,875,683 (GRCm39)		probably benign	Het
Suco	T	A	1: 161,662,752 (GRCm39)	K560*	probably null	Het
Tbx3	T	C	5: 119,819,018 (GRCm39)	V531A	probably benign	Het
Tmem30c	T	C	16: 57,098,100 (GRCm39)	Y107C	probably damaging	Het
Topbp1	T	A	9: 103,188,963 (GRCm39)		probably null	Het
Trpm5	A	T	7: 142,642,688 (GRCm39)	D86E	probably damaging	Het
Urb2	C	T	8: 124,755,300 (GRCm39)	Q336*	probably null	Het
Vmn2r43	A	G	7: 8,247,970 (GRCm39)	F731S	probably damaging	Het
Xkr9	A	G	1: 13,754,359 (GRCm39)	T118A	probably benign	Het
Zcchc2	A	C	1: 105,947,609 (GRCm39)	K117N	probably damaging	Het
Zfp52	T	C	17: 21,780,733 (GRCm39)	Y194H	probably damaging	Het
Zfp763	C	T	17: 33,237,675 (GRCm39)	C490Y	possibly damaging	Het
Zmynd19	G	T	2: 24,848,131 (GRCm39)	R148L	possibly damaging	Het

Other mutations in Pcdh15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00421:Pcdh15	APN	10	74,021,177 (GRCm39)	nonsense	probably null
IGL00432:Pcdh15	APN	10	74,126,914 (GRCm39)	splice site	probably benign
IGL00533:Pcdh15	APN	10	74,338,552 (GRCm39)	missense	probably damaging	1.00
IGL00596:Pcdh15	APN	10	74,466,576 (GRCm39)	missense	probably benign	0.00
IGL00930:Pcdh15	APN	10	74,466,530 (GRCm39)	missense	probably benign	0.08
IGL00970:Pcdh15	APN	10	74,215,172 (GRCm39)	missense	probably damaging	1.00
IGL01087:Pcdh15	APN	10	74,178,464 (GRCm39)	missense	possibly damaging	0.90
IGL01763:Pcdh15	APN	10	74,046,293 (GRCm39)	missense	probably benign	0.09
IGL01787:Pcdh15	APN	10	74,286,115 (GRCm39)	missense	probably benign	0.25
IGL02070:Pcdh15	APN	10	74,466,700 (GRCm39)	missense	probably benign	0.00
IGL02234:Pcdh15	APN	10	74,467,694 (GRCm39)	missense	probably benign	0.02
IGL02268:Pcdh15	APN	10	74,178,504 (GRCm39)	missense	probably damaging	1.00
IGL02280:Pcdh15	APN	10	74,058,295 (GRCm39)	missense	probably damaging	1.00
IGL02363:Pcdh15	APN	10	74,152,918 (GRCm39)	missense	probably damaging	0.98
IGL02420:Pcdh15	APN	10	74,138,938 (GRCm39)	missense	probably damaging	0.98
IGL02749:Pcdh15	APN	10	74,466,900 (GRCm39)	missense	probably benign	0.00
IGL02939:Pcdh15	APN	10	74,340,648 (GRCm39)	splice site	probably benign
IGL02970:Pcdh15	APN	10	74,126,794 (GRCm39)	splice site	probably benign
IGL03010:Pcdh15	APN	10	74,221,777 (GRCm39)	missense	probably damaging	1.00
IGL03061:Pcdh15	APN	10	74,152,843 (GRCm39)	missense	probably damaging	0.97
IGL03095:Pcdh15	APN	10	74,191,706 (GRCm39)	missense	probably damaging	1.00
IGL03149:Pcdh15	APN	10	74,466,527 (GRCm39)	missense	probably damaging	1.00
IGL03187:Pcdh15	APN	10	74,191,706 (GRCm39)	missense	probably damaging	1.00
IGL03279:Pcdh15	APN	10	74,152,904 (GRCm39)	missense	probably damaging	1.00
IGL03392:Pcdh15	APN	10	74,460,104 (GRCm39)	missense	probably damaging	1.00
loop	UTSW	10	74,021,210 (GRCm39)	missense	probably damaging	1.00
mcduck	UTSW	10	74,462,676 (GRCm39)	critical splice donor site	probably null
spaz	UTSW	10	74,046,257 (GRCm39)	missense	probably damaging	1.00
sphere	UTSW	10	74,460,116 (GRCm39)	missense	probably damaging	1.00
squirm	UTSW	10	0 ()	large deletion
Tortilla	UTSW	10	74,215,249 (GRCm39)	splice site	probably null
1mM(1):Pcdh15	UTSW	10	74,461,969 (GRCm39)	intron	probably benign
BB009:Pcdh15	UTSW	10	74,481,359 (GRCm39)	missense	probably benign	0.18
BB019:Pcdh15	UTSW	10	74,481,359 (GRCm39)	missense	probably benign	0.18
R0038:Pcdh15	UTSW	10	74,479,272 (GRCm39)	missense	possibly damaging	0.95
R0103:Pcdh15	UTSW	10	74,046,257 (GRCm39)	missense	probably damaging	1.00
R0110:Pcdh15	UTSW	10	74,126,808 (GRCm39)	missense	probably damaging	1.00
R0111:Pcdh15	UTSW	10	74,462,651 (GRCm39)	nonsense	probably null
R0119:Pcdh15	UTSW	10	74,006,407 (GRCm39)	missense	probably damaging	1.00
R0131:Pcdh15	UTSW	10	74,006,440 (GRCm39)	missense	probably null	1.00
R0445:Pcdh15	UTSW	10	74,178,381 (GRCm39)	missense	probably damaging	1.00
R0464:Pcdh15	UTSW	10	74,462,676 (GRCm39)	critical splice donor site	probably null
R0503:Pcdh15	UTSW	10	74,046,217 (GRCm39)	missense	probably damaging	1.00
R0507:Pcdh15	UTSW	10	74,457,129 (GRCm39)	missense	probably damaging	1.00
R0510:Pcdh15	UTSW	10	74,126,808 (GRCm39)	missense	probably damaging	1.00
R0742:Pcdh15	UTSW	10	74,457,129 (GRCm39)	missense	probably damaging	1.00
R0790:Pcdh15	UTSW	10	74,466,885 (GRCm39)	missense	probably benign	0.01
R0829:Pcdh15	UTSW	10	74,338,598 (GRCm39)	missense	probably damaging	1.00
R0839:Pcdh15	UTSW	10	74,462,614 (GRCm39)	missense	probably null	1.00
R0882:Pcdh15	UTSW	10	74,178,488 (GRCm39)	missense	probably damaging	1.00
R0894:Pcdh15	UTSW	10	74,460,087 (GRCm39)	missense	probably damaging	1.00
R0944:Pcdh15	UTSW	10	74,046,302 (GRCm39)	missense	probably damaging	0.99
R1081:Pcdh15	UTSW	10	74,286,145 (GRCm39)	missense	probably damaging	1.00
R1148:Pcdh15	UTSW	10	74,006,392 (GRCm39)	missense	probably damaging	1.00
R1148:Pcdh15	UTSW	10	74,006,392 (GRCm39)	missense	probably damaging	1.00
R1484:Pcdh15	UTSW	10	74,126,833 (GRCm39)	missense	probably damaging	1.00
R1521:Pcdh15	UTSW	10	74,430,023 (GRCm39)	missense	probably damaging	1.00
R1694:Pcdh15	UTSW	10	74,429,995 (GRCm39)	missense	probably damaging	1.00
R1795:Pcdh15	UTSW	10	74,460,087 (GRCm39)	missense	probably damaging	1.00
R2021:Pcdh15	UTSW	10	74,467,025 (GRCm39)	missense	possibly damaging	0.93
R2022:Pcdh15	UTSW	10	74,467,025 (GRCm39)	missense	possibly damaging	0.93
R2023:Pcdh15	UTSW	10	74,467,025 (GRCm39)	missense	possibly damaging	0.93
R2076:Pcdh15	UTSW	10	74,178,479 (GRCm39)	missense	probably damaging	1.00
R2199:Pcdh15	UTSW	10	74,006,341 (GRCm39)	missense	probably damaging	1.00
R2510:Pcdh15	UTSW	10	74,467,331 (GRCm39)	missense	probably benign	0.39
R2511:Pcdh15	UTSW	10	74,481,828 (GRCm39)	missense	possibly damaging	0.94
R3418:Pcdh15	UTSW	10	74,420,054 (GRCm39)	missense	probably benign	0.12
R3419:Pcdh15	UTSW	10	74,420,054 (GRCm39)	missense	probably benign	0.12
R3433:Pcdh15	UTSW	10	74,467,331 (GRCm39)	missense	probably benign	0.39
R3619:Pcdh15	UTSW	10	74,479,227 (GRCm39)	missense	probably benign	0.19
R3723:Pcdh15	UTSW	10	74,481,680 (GRCm39)	missense	probably benign	0.05
R3724:Pcdh15	UTSW	10	74,481,680 (GRCm39)	missense	probably benign	0.05
R3778:Pcdh15	UTSW	10	73,782,983 (GRCm39)	splice site	probably null
R3851:Pcdh15	UTSW	10	74,467,518 (GRCm39)	missense	probably damaging	0.97
R4175:Pcdh15	UTSW	10	74,467,829 (GRCm39)	intron	probably benign
R4261:Pcdh15	UTSW	10	74,481,512 (GRCm39)	missense	probably damaging	1.00
R4385:Pcdh15	UTSW	10	74,386,322 (GRCm39)	missense	probably damaging	1.00
R4585:Pcdh15	UTSW	10	74,460,116 (GRCm39)	missense	probably damaging	1.00
R4602:Pcdh15	UTSW	10	74,430,046 (GRCm39)	missense	probably damaging	1.00
R4639:Pcdh15	UTSW	10	74,479,439 (GRCm39)	missense	probably benign	0.00
R4703:Pcdh15	UTSW	10	74,285,995 (GRCm39)	missense	probably damaging	1.00
R4819:Pcdh15	UTSW	10	74,160,221 (GRCm39)	missense	probably damaging	1.00
R4906:Pcdh15	UTSW	10	74,340,625 (GRCm39)	nonsense	probably null
R4961:Pcdh15	UTSW	10	74,215,249 (GRCm39)	splice site	probably null
R5018:Pcdh15	UTSW	10	74,479,607 (GRCm39)	missense	possibly damaging	0.68
R5125:Pcdh15	UTSW	10	74,419,912 (GRCm39)	missense	probably damaging	0.98
R5225:Pcdh15	UTSW	10	74,138,986 (GRCm39)	missense	probably damaging	1.00
R5259:Pcdh15	UTSW	10	74,232,204 (GRCm39)	missense	possibly damaging	0.67
R5279:Pcdh15	UTSW	10	74,430,015 (GRCm39)	missense	probably damaging	1.00
R5395:Pcdh15	UTSW	10	74,021,119 (GRCm39)	missense	probably damaging	1.00
R5458:Pcdh15	UTSW	10	74,340,611 (GRCm39)	missense	probably damaging	1.00
R5617:Pcdh15	UTSW	10	74,471,504 (GRCm39)	intron	probably benign
R5665:Pcdh15	UTSW	10	74,462,620 (GRCm39)	missense	probably damaging	1.00
R5770:Pcdh15	UTSW	10	74,021,177 (GRCm39)	nonsense	probably null
R5805:Pcdh15	UTSW	10	74,066,091 (GRCm39)	missense	probably damaging	1.00
R5914:Pcdh15	UTSW	10	74,466,768 (GRCm39)	missense	probably benign	0.42
R5988:Pcdh15	UTSW	10	74,215,189 (GRCm39)	missense	probably benign	0.05
R6189:Pcdh15	UTSW	10	74,178,483 (GRCm39)	missense	probably null	1.00
R6414:Pcdh15	UTSW	10	74,021,258 (GRCm39)	missense	probably damaging	1.00
R6536:Pcdh15	UTSW	10	74,467,221 (GRCm39)	missense	probably damaging	1.00
R6612:Pcdh15	UTSW	10	74,021,210 (GRCm39)	missense	probably damaging	1.00
R6711:Pcdh15	UTSW	10	74,478,219 (GRCm39)	missense	possibly damaging	0.83
R6793:Pcdh15	UTSW	10	74,466,971 (GRCm39)	missense	probably damaging	1.00
R6841:Pcdh15	UTSW	10	74,286,052 (GRCm39)	missense	probably damaging	1.00
R6845:Pcdh15	UTSW	10	74,466,465 (GRCm39)	missense	probably benign
R6915:Pcdh15	UTSW	10	74,479,641 (GRCm39)	missense	probably benign	0.16
R6954:Pcdh15	UTSW	10	74,481,821 (GRCm39)	missense	possibly damaging	0.92
R6970:Pcdh15	UTSW	10	74,338,519 (GRCm39)	missense	probably damaging	0.98
R7018:Pcdh15	UTSW	10	74,302,186 (GRCm39)	missense	probably damaging	1.00
R7064:Pcdh15	UTSW	10	74,466,446 (GRCm39)	missense	possibly damaging	0.67
R7079:Pcdh15	UTSW	10	74,152,957 (GRCm39)	missense	probably benign	0.21
R7172:Pcdh15	UTSW	10	74,338,597 (GRCm39)	missense	probably damaging	1.00
R7220:Pcdh15	UTSW	10	74,178,441 (GRCm39)	missense	possibly damaging	0.64
R7237:Pcdh15	UTSW	10	74,420,023 (GRCm39)	missense	possibly damaging	0.88
R7266:Pcdh15	UTSW	10	74,215,222 (GRCm39)	nonsense	probably null
R7276:Pcdh15	UTSW	10	74,160,224 (GRCm39)	missense	probably benign	0.25
R7359:Pcdh15	UTSW	10	74,420,048 (GRCm39)	missense	probably damaging	0.99
R7396:Pcdh15	UTSW	10	74,466,522 (GRCm39)	missense	probably benign	0.17
R7421:Pcdh15	UTSW	10	74,289,897 (GRCm39)	missense	possibly damaging	0.90
R7424:Pcdh15	UTSW	10	74,342,317 (GRCm39)	missense	probably benign	0.09
R7463:Pcdh15	UTSW	10	74,467,602 (GRCm39)	missense	possibly damaging	0.66
R7469:Pcdh15	UTSW	10	74,481,812 (GRCm39)	missense	probably benign
R7512:Pcdh15	UTSW	10	74,477,214 (GRCm39)	missense	possibly damaging	0.81
R7767:Pcdh15	UTSW	10	74,322,088 (GRCm39)	missense	probably benign	0.07
R7830:Pcdh15	UTSW	10	74,221,733 (GRCm39)	missense	probably damaging	1.00
R7890:Pcdh15	UTSW	10	74,478,146 (GRCm39)	missense	probably damaging	1.00
R7897:Pcdh15	UTSW	10	74,289,827 (GRCm39)	missense	probably damaging	1.00
R7908:Pcdh15	UTSW	10	74,479,414 (GRCm39)	missense	probably benign	0.04
R7932:Pcdh15	UTSW	10	74,481,359 (GRCm39)	missense	probably benign	0.18
R7940:Pcdh15	UTSW	10	74,430,022 (GRCm39)	missense	probably damaging	1.00
R8230:Pcdh15	UTSW	10	74,191,707 (GRCm39)	missense	probably damaging	1.00
R8307:Pcdh15	UTSW	10	74,342,307 (GRCm39)	nonsense	probably null
R8382:Pcdh15	UTSW	10	74,479,227 (GRCm39)	missense	probably benign	0.19
R8397:Pcdh15	UTSW	10	74,126,865 (GRCm39)	missense	probably damaging	1.00
R8498:Pcdh15	UTSW	10	74,317,974 (GRCm39)	missense	probably damaging	1.00
R8692:Pcdh15	UTSW	10	74,289,805 (GRCm39)	missense	possibly damaging	0.63
R8797:Pcdh15	UTSW	10	74,419,978 (GRCm39)	missense	probably damaging	1.00
R9020:Pcdh15	UTSW	10	74,481,443 (GRCm39)	missense	probably benign	0.01
R9033:Pcdh15	UTSW	10	74,302,138 (GRCm39)	missense	probably damaging	1.00
R9056:Pcdh15	UTSW	10	74,221,731 (GRCm39)	missense	probably damaging	1.00
R9177:Pcdh15	UTSW	10	74,479,455 (GRCm39)	missense	probably benign	0.13
R9191:Pcdh15	UTSW	10	74,161,981 (GRCm39)	missense	probably benign	0.38
R9268:Pcdh15	UTSW	10	74,479,455 (GRCm39)	missense	probably benign	0.13
R9279:Pcdh15	UTSW	10	74,461,756 (GRCm39)	intron	probably benign
R9294:Pcdh15	UTSW	10	74,479,560 (GRCm39)	missense	unknown
R9387:Pcdh15	UTSW	10	74,066,192 (GRCm39)	missense	probably damaging	0.98
R9409:Pcdh15	UTSW	10	74,160,190 (GRCm39)	missense	probably damaging	0.98
R9410:Pcdh15	UTSW	10	74,481,663 (GRCm39)	frame shift	probably null
R9412:Pcdh15	UTSW	10	74,481,663 (GRCm39)	frame shift	probably null
R9432:Pcdh15	UTSW	10	74,460,170 (GRCm39)	missense	probably damaging	1.00
R9444:Pcdh15	UTSW	10	74,478,176 (GRCm39)	missense	probably damaging	1.00
R9579:Pcdh15	UTSW	10	74,457,117 (GRCm39)	missense	possibly damaging	0.89
R9643:Pcdh15	UTSW	10	74,479,335 (GRCm39)	missense	probably benign	0.18
R9784:Pcdh15	UTSW	10	74,467,212 (GRCm39)	missense	probably benign	0.00
RF020:Pcdh15	UTSW	10	74,021,242 (GRCm39)	missense	probably damaging	1.00
Z1176:Pcdh15	UTSW	10	74,466,533 (GRCm39)	missense	probably benign	0.00
Z1177:Pcdh15	UTSW	10	74,340,632 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAATGTGCCCCTGAACACAC -3'
(R):5'- AGCAGATCATTCGAAGTGTTAGG -3'

Sequencing Primer
(F):5'- ACACACTGTTCAAGGGGC -3'
(R):5'- GGTTGTGCACATGTGATAAACCCC -3'

Posted On

2017-10-10