Mutagenetix > Incidental Mutations

Incidental Mutation 'R6179:Cry2'

487918

Institutional Source

Beutler Lab

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome 2 (photolyase-like)

Synonyms

MMRRC Submission

044321-MU

Accession Numbers

Is this an essential gene?

Possibly essential (E-score: 0.559) question?

Stock #

R6179 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

92403646-92434043 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 92413842 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 329 (G329R)

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

AlphaFold

Q9R194

Predicted Effect

probably damaging
Transcript: ENSMUST00000090559
AA Change: G329R

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: G329R

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000111278
AA Change: G329R

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: G329R

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.5%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930426L09Rik	T	C	2: 18,998,842		probably benign	Het
4930590J08Rik	T	C	6: 91,942,330	S657P	probably damaging	Het
Abca9	T	C	11: 110,134,254	I988V	probably benign	Het
Aox4	T	A	1: 58,231,503	D280E	probably benign	Het
Apob	T	A	12: 8,005,060	L1353*	probably null	Het
Atp2b1	C	T	10: 99,022,829	R222C	probably damaging	Het
AW209491	T	G	13: 14,637,083	S174A	possibly damaging	Het
B930094E09Rik	G	C	18: 31,609,858		probably benign	Het
Cbln3	G	T	14: 55,884,060	P43Q	possibly damaging	Het
Ccdc181	A	T	1: 164,279,918	T57S	probably benign	Het
Ccr2	A	C	9: 124,105,971	N96T	probably damaging	Het
Cep131	T	C	11: 120,066,011	I868V	probably benign	Het
Chd2	A	G	7: 73,444,323	I1535T	probably damaging	Het
Chl1	A	T	6: 103,683,243	T377S	probably benign	Het
Cpeb3	A	G	19: 37,088,453	F439L	probably damaging	Het
Crb2	T	C	2: 37,790,257	V399A	probably damaging	Het
Dock4	A	G	12: 40,731,869	E691G	probably benign	Het
Edil3	A	T	13: 88,821,989	H3L	probably benign	Het
Endod1	T	C	9: 14,357,461	I243V	probably benign	Het
Fpr3	G	A	17: 17,970,657	W63*	probably null	Het
Gm498	A	G	7: 143,871,787	T58A	probably benign	Het
Gtse1	T	G	15: 85,868,957	N424K	possibly damaging	Het
Kcnt1	G	A	2: 25,893,180	V252M	probably damaging	Het
Kif1bp	G	A	10: 62,563,250	Q130*	probably null	Het
Lmbrd2	T	G	15: 9,149,175	I48M	probably damaging	Het
Lrrc7	T	G	3: 158,353,432	I13L	probably damaging	Het
Mbd4	A	G	6: 115,845,425	S408P	probably benign	Het
Mphosph10	A	G	7: 64,378,781	V542A	possibly damaging	Het
Msto1	C	A	3: 88,910,947	R331L	probably damaging	Het
Myh10	G	A	11: 68,802,153	E1425K	probably damaging	Het
Nup43	T	C	10: 7,678,673	I340T	probably benign	Het
Olfr350	T	C	2: 36,850,834	S263P	possibly damaging	Het
Pcm1	T	A	8: 41,283,632	M884K	probably damaging	Het
Phf20	T	A	2: 156,298,653	L749H	probably damaging	Het
Pigp	T	C	16: 94,370,367	R22G	probably null	Het
Polr2b	T	C	5: 77,320,977	M200T	probably damaging	Het
Ppig	T	A	2: 69,750,127	D668E	unknown	Het
Prss3	G	A	6: 41,375,126	R68C	probably benign	Het
Retreg3	G	A	11: 101,103,895		probably benign	Het
Shank1	T	C	7: 44,357,206	F2116L	possibly damaging	Het
Spg21	T	C	9: 65,468,808	S33P	possibly damaging	Het
Spns3	A	G	11: 72,499,523	S432P	probably damaging	Het
Stra6	A	G	9: 58,135,169	E27G	probably damaging	Het
Tanc1	A	G	2: 59,842,976	Q1468R	probably benign	Het
Tbce	T	G	13: 14,019,777	E99A	probably benign	Het
Tmem106b	T	C	6: 13,084,253	V252A	probably damaging	Het
Trim10	A	G	17: 36,877,031	T380A	probably damaging	Het
Trim31	T	A	17: 36,909,609	D472E	probably damaging	Het
Vmn2r28	G	A	7: 5,488,004	Q415*	probably null	Het
Vwf	G	A	6: 125,649,289	C7Y	unknown	Het
Yeats2	C	T	16: 20,214,475	P1088L	probably benign	Het
Zfp40	C	A	17: 23,178,380	V48L	possibly damaging	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01995:Cry2	APN	2	92424632	missense	probably benign	0.15
IGL02167:Cry2	APN	2	92433821	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92413039	missense	probably damaging	0.99
IGL02343:Cry2	APN	2	92426921	missense	possibly damaging	0.90
IGL02432:Cry2	APN	2	92413667	missense	probably damaging	0.99
IGL02725:Cry2	APN	2	92413260	splice site	probably benign
IGL02932:Cry2	APN	2	92413117	nonsense	probably null
IGL03122:Cry2	APN	2	92413295	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92413715	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R1325:Cry2	UTSW	2	92413770	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92424566	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92413640	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92411692	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92413827	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92424554	missense	probably benign	0.38
R5130:Cry2	UTSW	2	92424599	missense	probably benign	0.28
R5145:Cry2	UTSW	2	92413060	missense	probably benign
R5970:Cry2	UTSW	2	92412967	missense	probably benign	0.08
R7102:Cry2	UTSW	2	92413093	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92413659	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92412981	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92413664	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92413638	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92413005	missense	probably damaging	0.98
R8234:Cry2	UTSW	2	92412629	missense	probably benign
R8350:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8450:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8496:Cry2	UTSW	2	92426939	missense	probably damaging	1.00
R9172:Cry2	UTSW	2	92413648	missense	probably damaging	1.00
R9283:Cry2	UTSW	2	92413904	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAGGGTACAGGCTAGCTTG -3'
(R):5'- CATCGTCACAGTGCTAAGAGC -3'

Sequencing Primer
(F):5'- GTACAGGCTAGCTTGCACTCAC -3'
(R):5'- CCAGGCAGACACTCATCTCTGTG -3'

Posted On

2017-10-10