|Institutional Source||Beutler Lab|
|Gene Name||selenoprotein T|
|Synonyms||Selt, 5730408P04Rik, SelT, 2810407C02Rik|
|Is this an essential gene?||Possibly non essential (E-score: 0.322)|
|Stock #||R6137 (G1)|
|Chromosomal Location||58576636-58593133 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 58585284 bp|
|Amino Acid Change||Glutamine to Leucine at position 64 (Q64L)|
|Ref Sequence||ENSEMBL: ENSMUSP00000103557 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000107924]|
|Predicted Effect||probably damaging
AA Change: Q64L
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: Q64L
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.3705|
|Coding Region Coverage||
|Validation Efficiency||98% (58/59)|
FUNCTION: This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a conditional allele activated in beta cells exhibit impaired glucose tolerance, increased circulating glucose levels, decreased circulating insulin levels, decreased insulin secretion and an increase in smaller islets. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Selenot||
(F):5'- GCCCTGTATCTGTTGTTGAGTTC -3'
(R):5'- TCATCCCGTGTATTATTTACAAAGTAG -3'
(F):5'- CGTAGAGTTTACTTGTCTTTAAG -3'
(R):5'- AACCAGCAAGGGTGACTA -3'