Incidental Mutation 'R6145:Srsf4'
ID 488805
Institutional Source Beutler Lab
Gene Symbol Srsf4
Ensembl Gene ENSMUSG00000028911
Gene Name serine/arginine-rich splicing factor 4
Synonyms MNCb-2616, Sfrs4, SRp75, 5730499P16Rik
MMRRC Submission 044292-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.372) question?
Stock # R6145 (G1)
Quality Score 225.009
Status Not validated
Chromosome 4
Chromosomal Location 131873617-131901706 bp(+) (GRCm38)
Type of Mutation unclassified
DNA Base Change (assembly) G to T at 131900294 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000142148 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053819] [ENSMUST00000134943]
AlphaFold no structure available at present
Predicted Effect noncoding transcript
Transcript: ENSMUST00000030743
SMART Domains Protein: ENSMUSP00000030743
Gene: ENSMUSG00000028911

RRM 14 73 1.09e0 SMART
low complexity region 76 102 N/A INTRINSIC
RRM 110 178 2e-14 SMART
low complexity region 184 277 N/A INTRINSIC
low complexity region 304 328 N/A INTRINSIC
low complexity region 329 408 N/A INTRINSIC
low complexity region 460 496 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000053819
AA Change: R233L
SMART Domains Protein: ENSMUSP00000061474
Gene: ENSMUSG00000028911
AA Change: R233L

RRM 3 68 4.3e-21 SMART
low complexity region 71 97 N/A INTRINSIC
RRM 105 173 8.4e-17 SMART
low complexity region 179 272 N/A INTRINSIC
low complexity region 299 323 N/A INTRINSIC
low complexity region 324 403 N/A INTRINSIC
low complexity region 455 490 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000121250
Predicted Effect noncoding transcript
Transcript: ENSMUST00000124738
Predicted Effect probably benign
Transcript: ENSMUST00000134943
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 98.0%
  • 20x: 95.0%
Validation Efficiency
MGI Phenotype FUNCTION: The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. [provided by RefSeq, Sep 2010]
Allele List at MGI
Other mutations in this stock
Total: 77 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2810459M11Rik T A 1: 86,052,942 (GRCm38) probably null Het
Abca8b A G 11: 109,973,808 (GRCm38) V316A probably benign Het
Acad10 A T 5: 121,622,033 (GRCm38) V999D probably damaging Het
Acot8 A G 2: 164,803,065 (GRCm38) V66A probably benign Het
Ankrd11 T C 8: 122,892,661 (GRCm38) H1484R probably damaging Het
Anxa6 A G 11: 54,994,904 (GRCm38) F405S probably damaging Het
Asmt G A X: 170,674,663 (GRCm38) V101I probably damaging Het
Atp1a2 C T 1: 172,287,238 (GRCm38) V327I probably damaging Het
Brdt A G 5: 107,377,999 (GRCm38) E906G possibly damaging Het
Cacna1g A T 11: 94,462,261 (GRCm38) C313S probably damaging Het
Camk2d T C 3: 126,805,858 (GRCm38) I329T probably benign Het
Cavin4 A T 4: 48,663,794 (GRCm38) H58L probably damaging Het
Ccdc78 C A 17: 25,789,065 (GRCm38) P317T probably benign Het
Cdc16 T G 8: 13,767,573 (GRCm38) Y295D possibly damaging Het
Cdyl2 T A 8: 116,594,978 (GRCm38) N270I probably damaging Het
Cfap221 T A 1: 119,984,816 (GRCm38) I114F possibly damaging Het
Dmxl1 A G 18: 49,912,766 (GRCm38) E2414G possibly damaging Het
Dnaaf9 T C 2: 130,778,473 (GRCm38) I247V probably benign Het
Dnah1 C A 14: 31,300,970 (GRCm38) R1070L probably benign Het
Dnah14 T C 1: 181,666,417 (GRCm38) S1713P probably benign Het
Dock10 C A 1: 80,575,904 (GRCm38) G602* probably null Het
Ep400 A T 5: 110,756,703 (GRCm38) V10D possibly damaging Het
Epas1 T C 17: 86,829,429 (GRCm38) C807R probably benign Het
Esrrb C T 12: 86,505,899 (GRCm38) P200L probably benign Het
Fbxw26 T C 9: 109,732,623 (GRCm38) I168V probably benign Het
Fsip2 A T 2: 82,993,768 (GRCm38) H6615L possibly damaging Het
Galk2 A T 2: 125,946,842 (GRCm38) Q272L possibly damaging Het
Gas7 A C 11: 67,629,612 (GRCm38) T43P probably damaging Het
Gm5134 A T 10: 75,995,839 (GRCm38) I371F probably damaging Het
Gpr31b C T 17: 13,051,379 (GRCm38) R301Q possibly damaging Het
Grk1 T A 8: 13,405,765 (GRCm38) Y216* probably null Het
Grm5 T A 7: 88,026,601 (GRCm38) M441K probably damaging Het
Heatr6 A G 11: 83,766,136 (GRCm38) E408G probably damaging Het
Hoxc9 G T 15: 102,983,959 (GRCm38) K201N probably damaging Het
Igsf10 T A 3: 59,331,656 (GRCm38) Y368F possibly damaging Het
Il2ra A T 2: 11,680,246 (GRCm38) D131V probably damaging Het
Imp4 A G 1: 34,440,096 (GRCm38) E19G probably benign Het
Kcnk18 T C 19: 59,235,607 (GRCm38) *395Q probably null Het
Kdm6b A T 11: 69,405,026 (GRCm38) L805Q unknown Het
Lgr4 T A 2: 110,007,243 (GRCm38) L427* probably null Het
Myt1l G A 12: 29,832,381 (GRCm38) S525N unknown Het
Nasp G A 4: 116,611,077 (GRCm38) T237I probably benign Het
Nell2 G T 15: 95,473,561 (GRCm38) Q98K probably damaging Het
Nfasc C T 1: 132,634,717 (GRCm38) G107R probably damaging Het
Nsun4 A G 4: 116,040,206 (GRCm38) S203P probably damaging Het
Or1m1 T G 9: 18,755,569 (GRCm38) D22A probably benign Het
Or7g35 T C 9: 19,584,888 (GRCm38) V117A probably benign Het
Otogl G A 10: 107,777,117 (GRCm38) silent Het
Pde10a T C 17: 8,929,117 (GRCm38) V366A probably damaging Het
Pdxk T A 10: 78,443,791 (GRCm38) D250V probably benign Het
Pih1d1 T A 7: 45,159,044 (GRCm38) I179N probably damaging Het
Plaa T A 4: 94,583,992 (GRCm38) I294F probably damaging Het
Pmel C A 10: 128,715,935 (GRCm38) P213T probably damaging Het
Pom121l2 A T 13: 21,982,302 (GRCm38) R248* probably null Het
Pou2f1 T C 1: 165,875,433 (GRCm38) probably benign Het
Ppm1j G A 3: 104,781,379 (GRCm38) R98H probably damaging Het
Prkdc C T 16: 15,772,073 (GRCm38) P2600L probably damaging Het
Prom1 T C 5: 44,029,649 (GRCm38) N422S probably benign Het
Pspn C T 17: 56,999,467 (GRCm38) C154Y probably damaging Het
Ptdss1 T C 13: 66,972,637 (GRCm38) probably null Het
Rapgef1 A G 2: 29,736,666 (GRCm38) Y993C probably damaging Het
Scrn2 A C 11: 97,032,853 (GRCm38) T219P probably benign Het
Sec23ip T G 7: 128,778,484 (GRCm38) S874R probably damaging Het
Septin4 G A 11: 87,585,246 (GRCm38) probably null Het
Slc15a3 C T 19: 10,857,251 (GRCm38) L499F probably damaging Het
Spaca9 G A 2: 28,693,781 (GRCm38) R64W probably damaging Het
Sra1 A G 18: 36,667,575 (GRCm38) M193T probably damaging Het
Syne1 T C 10: 5,052,750 (GRCm38) D8055G probably damaging Het
Syne4 T A 7: 30,316,563 (GRCm38) probably null Het
Tbc1d24 C A 17: 24,208,229 (GRCm38) G253V probably damaging Het
Tbck T A 3: 132,732,215 (GRCm38) I467N probably damaging Het
Tln1 T A 4: 43,538,030 (GRCm38) M1857L possibly damaging Het
Ttll2 T C 17: 7,351,632 (GRCm38) R299G probably benign Het
Ugt2b36 T G 5: 87,066,213 (GRCm38) E524A probably benign Het
Vmn2r124 C T 17: 18,062,851 (GRCm38) T269I probably benign Het
Vmn2r4 A G 3: 64,406,943 (GRCm38) F206L probably benign Het
Zfp346 A G 13: 55,115,574 (GRCm38) K156R probably damaging Het
Other mutations in Srsf4
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0087:Srsf4 UTSW 4 131,900,330 (GRCm38) unclassified probably benign
R1135:Srsf4 UTSW 4 131,900,069 (GRCm38) unclassified probably benign
R1209:Srsf4 UTSW 4 131,901,059 (GRCm38) unclassified probably benign
R1276:Srsf4 UTSW 4 131,897,685 (GRCm38) missense probably damaging 1.00
R1561:Srsf4 UTSW 4 131,897,695 (GRCm38) missense probably damaging 1.00
R1574:Srsf4 UTSW 4 131,897,695 (GRCm38) missense probably damaging 1.00
R1700:Srsf4 UTSW 4 131,900,560 (GRCm38) unclassified probably benign
R2265:Srsf4 UTSW 4 131,897,682 (GRCm38) missense probably damaging 1.00
R2269:Srsf4 UTSW 4 131,897,682 (GRCm38) missense probably damaging 1.00
R3723:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R3724:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R3737:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R3738:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R3739:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R4034:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R4035:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R4049:Srsf4 UTSW 4 131,900,543 (GRCm38) unclassified probably benign
R4535:Srsf4 UTSW 4 131,873,864 (GRCm38) missense probably damaging 1.00
R4810:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R4833:Srsf4 UTSW 4 131,900,102 (GRCm38) unclassified probably benign
R4932:Srsf4 UTSW 4 131,891,245 (GRCm38) missense probably damaging 0.99
R5291:Srsf4 UTSW 4 131,886,306 (GRCm38) critical splice donor site probably benign
R5725:Srsf4 UTSW 4 131,900,951 (GRCm38) unclassified probably benign
R7056:Srsf4 UTSW 4 131,900,693 (GRCm38) unclassified probably benign
R7294:Srsf4 UTSW 4 131,900,461 (GRCm38) missense unknown
R7964:Srsf4 UTSW 4 131,891,233 (GRCm38) missense probably benign 0.09
R8697:Srsf4 UTSW 4 131,900,731 (GRCm38) missense unknown
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2017-10-10