Mutagenetix > Incidental Mutation

Incidental Mutation 'R6145:Olfr24'

488818

Institutional Source

Beutler Lab

Gene Symbol

Olfr24

Ensembl Gene

ENSMUSG00000054141

Gene Name

olfactory receptor 24

Synonyms

MOR132-1, GA_x6K02T2PVTD-12498544-12497603, MTPCR51

MMRRC Submission

044292-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R6145 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

18751507-18763996 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 18755569 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 22 (D22A)

Ref Sequence

ENSEMBL: ENSMUSP00000149702 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000066997] [ENSMUST00000216754]

AlphaFold

Q8VFM9

Predicted Effect

probably benign
Transcript: ENSMUST00000066997
AA Change: D22A

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000064189
Gene: ENSMUSG00000054141
AA Change: D22A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	5.6e-64	PFAM
Pfam:7TM_GPCR_Srsx	35	304	9.2e-9	PFAM
Pfam:7tm_1	41	290	3.2e-26	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212239
AA Change: D22A

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214366

Predicted Effect

probably benign
Transcript: ENSMUST00000216754
AA Change: D22A

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 98.0%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810459M11Rik	T	A	1: 86,052,942		probably null	Het
4930402H24Rik	T	C	2: 130,778,473	I247V	probably benign	Het
Abca8b	A	G	11: 109,973,808	V316A	probably benign	Het
Acad10	A	T	5: 121,622,033	V999D	probably damaging	Het
Acot8	A	G	2: 164,803,065	V66A	probably benign	Het
Ankrd11	T	C	8: 122,892,661	H1484R	probably damaging	Het
Anxa6	A	G	11: 54,994,904	F405S	probably damaging	Het
Asmt	G	A	X: 170,674,663	V101I	probably damaging	Het
Atp1a2	C	T	1: 172,287,238	V327I	probably damaging	Het
Brdt	A	G	5: 107,377,999	E906G	possibly damaging	Het
Cacna1g	A	T	11: 94,462,261	C313S	probably damaging	Het
Camk2d	T	C	3: 126,805,858	I329T	probably benign	Het
Cavin4	A	T	4: 48,663,794	H58L	probably damaging	Het
Ccdc78	C	A	17: 25,789,065	P317T	probably benign	Het
Cdc16	T	G	8: 13,767,573	Y295D	possibly damaging	Het
Cdyl2	T	A	8: 116,594,978	N270I	probably damaging	Het
Cfap221	T	A	1: 119,984,816	I114F	possibly damaging	Het
Dmxl1	A	G	18: 49,912,766	E2414G	possibly damaging	Het
Dnah1	C	A	14: 31,300,970	R1070L	probably benign	Het
Dnah14	T	C	1: 181,666,417	S1713P	probably benign	Het
Dock10	C	A	1: 80,575,904	G602*	probably null	Het
Ep400	A	T	5: 110,756,703	V10D	possibly damaging	Het
Epas1	T	C	17: 86,829,429	C807R	probably benign	Het
Esrrb	C	T	12: 86,505,899	P200L	probably benign	Het
Fbxw26	T	C	9: 109,732,623	I168V	probably benign	Het
Fsip2	A	T	2: 82,993,768	H6615L	possibly damaging	Het
Galk2	A	T	2: 125,946,842	Q272L	possibly damaging	Het
Gas7	A	C	11: 67,629,612	T43P	probably damaging	Het
Gm5134	A	T	10: 75,995,839	I371F	probably damaging	Het
Gpr31b	C	T	17: 13,051,379	R301Q	possibly damaging	Het
Grk1	T	A	8: 13,405,765	Y216*	probably null	Het
Grm5	T	A	7: 88,026,601	M441K	probably damaging	Het
Heatr6	A	G	11: 83,766,136	E408G	probably damaging	Het
Hoxc9	G	T	15: 102,983,959	K201N	probably damaging	Het
Igsf10	T	A	3: 59,331,656	Y368F	possibly damaging	Het
Il2ra	A	T	2: 11,680,246	D131V	probably damaging	Het
Imp4	A	G	1: 34,440,096	E19G	probably benign	Het
Kcnk18	T	C	19: 59,235,607	*395Q	probably null	Het
Kdm6b	A	T	11: 69,405,026	L805Q	unknown	Het
Lgr4	T	A	2: 110,007,243	L427*	probably null	Het
Myt1l	G	A	12: 29,832,381	S525N	unknown	Het
Nasp	G	A	4: 116,611,077	T237I	probably benign	Het
Nell2	G	T	15: 95,473,561	Q98K	probably damaging	Het
Nfasc	C	T	1: 132,634,717	G107R	probably damaging	Het
Nsun4	A	G	4: 116,040,206	S203P	probably damaging	Het
Olfr855	T	C	9: 19,584,888	V117A	probably benign	Het
Otogl	G	A	10: 107,777,117		silent	Het
Pde10a	T	C	17: 8,929,117	V366A	probably damaging	Het
Pdxk	T	A	10: 78,443,791	D250V	probably benign	Het
Pih1d1	T	A	7: 45,159,044	I179N	probably damaging	Het
Plaa	T	A	4: 94,583,992	I294F	probably damaging	Het
Pmel	C	A	10: 128,715,935	P213T	probably damaging	Het
Pom121l2	A	T	13: 21,982,302	R248*	probably null	Het
Pou2f1	T	C	1: 165,875,433		probably benign	Het
Ppm1j	G	A	3: 104,781,379	R98H	probably damaging	Het
Prkdc	C	T	16: 15,772,073	P2600L	probably damaging	Het
Prom1	T	C	5: 44,029,649	N422S	probably benign	Het
Pspn	C	T	17: 56,999,467	C154Y	probably damaging	Het
Ptdss1	T	C	13: 66,972,637		probably null	Het
Rapgef1	A	G	2: 29,736,666	Y993C	probably damaging	Het
Scrn2	A	C	11: 97,032,853	T219P	probably benign	Het
Sec23ip	T	G	7: 128,778,484	S874R	probably damaging	Het
Sept4	G	A	11: 87,585,246		probably null	Het
Slc15a3	C	T	19: 10,857,251	L499F	probably damaging	Het
Spaca9	G	A	2: 28,693,781	R64W	probably damaging	Het
Sra1	A	G	18: 36,667,575	M193T	probably damaging	Het
Srsf4	G	T	4: 131,900,294		probably benign	Het
Syne1	T	C	10: 5,052,750	D8055G	probably damaging	Het
Syne4	T	A	7: 30,316,563		probably null	Het
Tbc1d24	C	A	17: 24,208,229	G253V	probably damaging	Het
Tbck	T	A	3: 132,732,215	I467N	probably damaging	Het
Tln1	T	A	4: 43,538,030	M1857L	possibly damaging	Het
Ttll2	T	C	17: 7,351,632	R299G	probably benign	Het
Ugt2b36	T	G	5: 87,066,213	E524A	probably benign	Het
Vmn2r124	C	T	17: 18,062,851	T269I	probably benign	Het
Vmn2r4	A	G	3: 64,406,943	F206L	probably benign	Het
Zfp346	A	G	13: 55,115,574	K156R	probably damaging	Het

Other mutations in Olfr24

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02346:Olfr24	APN	9	18754769	missense	probably damaging	0.97
IGL02371:Olfr24	APN	9	18754704	missense	probably benign	0.07
IGL02555:Olfr24	APN	9	18755473	missense	probably benign	0.40
IGL03025:Olfr24	APN	9	18755369	missense	probably benign	0.00
IGL03259:Olfr24	APN	9	18755515	missense	probably benign
IGL03050:Olfr24	UTSW	9	18755454	nonsense	probably null
R0310:Olfr24	UTSW	9	18755333	missense	possibly damaging	0.91
R0410:Olfr24	UTSW	9	18754841	missense	probably damaging	0.96
R2152:Olfr24	UTSW	9	18755095	missense	probably damaging	1.00
R2153:Olfr24	UTSW	9	18755095	missense	probably damaging	1.00
R2918:Olfr24	UTSW	9	18755479	missense	probably damaging	1.00
R4552:Olfr24	UTSW	9	18755134	missense	possibly damaging	0.60
R5073:Olfr24	UTSW	9	18754822	missense	possibly damaging	0.89
R5539:Olfr24	UTSW	9	18754838	missense	probably damaging	0.99
R5662:Olfr24	UTSW	9	18755600	missense	probably damaging	0.99
R7125:Olfr24	UTSW	9	18754878	nonsense	probably null
R7196:Olfr24	UTSW	9	18755590	nonsense	probably null
R7607:Olfr24	UTSW	9	18754882	missense	possibly damaging	0.93
R9136:Olfr24	UTSW	9	18754879	missense	probably damaging	1.00
Z1177:Olfr24	UTSW	9	18755519	missense	possibly damaging	0.85

Predicted Primers

PCR Primer
(F):5'- ACATCTGGGTCAGACAACAAG -3'
(R):5'- GTAATTAGCTCAGACAAGAACTGG -3'

Sequencing Primer
(F):5'- TATTCACCAGCATCTTGGGGACAG -3'
(R):5'- GAGTTAAACACATGGAACCAC -3'

Posted On

2017-10-10