Mutagenetix > Incidental Mutation

Incidental Mutation 'R6146:Or5w17'

488864

Institutional Source

Beutler Lab

Gene Symbol

Or5w17

Ensembl Gene

ENSMUSG00000075148

Gene Name

olfactory receptor family 5 subfamily W member 17, pseudogene 1

Synonyms

Olfr1141, GA_x6K02T2Q125-49257818-49256883, MOR177-10

MMRRC Submission

044293-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.139) question?

Stock #

R6146 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

87583400-87584335 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 87583602 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Arginine at position 245 (L245R)

Ref Sequence

ENSEMBL: ENSMUSP00000097433 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099846]

AlphaFold

Q8VFQ7

Predicted Effect

probably damaging
Transcript: ENSMUST00000099846
AA Change: L245R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097433
Gene: ENSMUSG00000075148
AA Change: L245R

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	2.8e-28	PFAM
Pfam:7tm_1	41	290	1.5e-6	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb4	A	G	5: 8,946,587 (GRCm39)	E29G	probably benign	Het
Abi3bp	T	C	16: 56,491,628 (GRCm39)	S552P	probably damaging	Het
Adgrg7	A	G	16: 56,593,829 (GRCm39)	I129T	probably benign	Het
Adhfe1	A	G	1: 9,623,943 (GRCm39)	N148S	probably damaging	Het
AI182371	A	G	2: 34,987,983 (GRCm39)	Y77H	probably damaging	Het
Aldh5a1	C	T	13: 25,103,661 (GRCm39)		probably null	Het
Ankrd2	A	G	19: 42,028,544 (GRCm39)	T67A	possibly damaging	Het
Anln	A	T	9: 22,287,604 (GRCm39)	C232*	probably null	Het
Cchcr1	A	G	17: 35,839,475 (GRCm39)	D587G	possibly damaging	Het
Cgn	G	T	3: 94,674,435 (GRCm39)	Q901K	possibly damaging	Het
Cip2a	A	T	16: 48,814,692 (GRCm39)	K18*	probably null	Het
Cluh	G	T	11: 74,558,054 (GRCm39)		probably null	Het
Crebbp	G	A	16: 3,902,487 (GRCm39)	Q2213*	probably null	Het
Cyp2d22	T	A	15: 82,258,036 (GRCm39)		probably null	Het
Dcdc2a	T	C	13: 25,389,440 (GRCm39)	V456A	probably benign	Het
Depdc5	A	G	5: 33,126,075 (GRCm39)	E569G	probably benign	Het
Dnah5	T	C	15: 28,459,331 (GRCm39)	F4517L	probably benign	Het
Doc2b	T	C	11: 75,664,421 (GRCm39)	K317E	probably damaging	Het
Dysf	T	A	6: 84,180,181 (GRCm39)	D1951E	probably damaging	Het
Epha6	C	A	16: 60,245,198 (GRCm39)	A334S	possibly damaging	Het
F2rl2	A	C	13: 95,837,149 (GRCm39)	I65L	probably benign	Het
Fbxw17	A	G	13: 50,586,548 (GRCm39)	K417E	probably benign	Het
Fkbpl	G	A	17: 34,864,303 (GRCm39)	A24T	probably benign	Het
Fxr2	A	G	11: 69,532,165 (GRCm39)	M96V	possibly damaging	Het
Kcnj10	C	A	1: 172,196,892 (GRCm39)	Y135*	probably null	Het
Kidins220	G	A	12: 25,102,812 (GRCm39)	R1238Q	probably damaging	Het
Krt31	T	C	11: 99,939,056 (GRCm39)	N255S	probably benign	Het
Lrp2	T	C	2: 69,341,345 (GRCm39)	D945G	probably benign	Het
Lrrc66	T	C	5: 73,765,432 (GRCm39)	D537G	probably benign	Het
Ltbp4	T	A	7: 27,019,149 (GRCm39)	I992F	probably damaging	Het
Lzts1	A	G	8: 69,593,524 (GRCm39)	S28P	probably benign	Het
Mrc2	C	A	11: 105,216,470 (GRCm39)	N86K	probably damaging	Het
Mroh6	C	A	15: 75,758,486 (GRCm39)	A302S	possibly damaging	Het
Muc16	T	A	9: 18,409,093 (GRCm39)	N198Y	probably damaging	Het
Myo9a	T	C	9: 59,778,512 (GRCm39)	S1423P	probably benign	Het
Or10ag58	A	T	2: 87,265,662 (GRCm39)	D277V	possibly damaging	Het
Or2b28	A	T	13: 21,531,164 (GRCm39)	Y22F	possibly damaging	Het
Or5h22	A	G	16: 58,895,077 (GRCm39)	V122A	probably benign	Het
Or5p56	G	T	7: 107,589,620 (GRCm39)	G16V	probably damaging	Het
Or9g4	T	A	2: 85,504,938 (GRCm39)	K186*	probably null	Het
Otogl	G	A	10: 107,612,978 (GRCm39)		silent	Het
Polg	T	C	7: 79,100,260 (GRCm39)	M1184V	probably benign	Het
Prl8a8	T	C	13: 27,694,463 (GRCm39)	Y108C	probably damaging	Het
Proser1	T	A	3: 53,385,540 (GRCm39)	I474N	probably damaging	Het
Rab44	A	G	17: 29,354,391 (GRCm39)		probably benign	Het
Rbp1	C	A	9: 98,307,669 (GRCm39)	D79E	possibly damaging	Het
Rnf213	T	C	11: 119,326,825 (GRCm39)	V1605A	probably benign	Het
Rps24	A	T	14: 24,540,803 (GRCm39)		probably null	Het
Setd5	T	C	6: 113,098,773 (GRCm39)		probably null	Het
Skic3	A	G	13: 76,333,359 (GRCm39)	E1536G	probably damaging	Het
Slc38a3	T	C	9: 107,532,228 (GRCm39)	I435V	probably benign	Het
Slco1a5	T	A	6: 142,180,534 (GRCm39)	M623L	probably benign	Het
Spaca9	G	A	2: 28,583,793 (GRCm39)	R64W	probably damaging	Het
Spn	A	G	7: 126,735,479 (GRCm39)	S343P	possibly damaging	Het
Sptan1	A	G	2: 29,894,535 (GRCm39)	T1168A	probably benign	Het
Sptbn4	A	T	7: 27,064,012 (GRCm39)	L2138*	probably null	Het
Tg	T	A	15: 66,545,216 (GRCm39)		probably null	Het
Tigd5	T	A	15: 75,782,094 (GRCm39)	L152Q	probably damaging	Het
Tmem163	C	T	1: 127,447,126 (GRCm39)	V170I	probably benign	Het
Tpr	T	C	1: 150,298,913 (GRCm39)	C1068R	possibly damaging	Het
Ubr1	A	T	2: 120,723,690 (GRCm39)	Y1290N	probably damaging	Het
Vmn1r184	T	A	7: 25,966,817 (GRCm39)	F188I	probably benign	Het
Vmn2r100	G	A	17: 19,742,522 (GRCm39)	V299I	probably benign	Het
Vmn2r91	T	G	17: 18,356,518 (GRCm39)	H728Q	probably benign	Het
Vta1	T	C	10: 14,581,096 (GRCm39)	Y37C	probably damaging	Het
Wbp2	C	A	11: 115,974,728 (GRCm39)	M35I	probably benign	Het
Zcchc9	G	A	13: 91,953,986 (GRCm39)	Q90*	probably null	Het

Other mutations in Or5w17

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01143:Or5w17	APN	2	87,584,278 (GRCm39)	missense	probably benign	0.00
IGL01412:Or5w17	APN	2	87,583,461 (GRCm39)	missense	probably damaging	1.00
IGL01533:Or5w17	APN	2	87,583,412 (GRCm39)	missense	probably benign	0.01
IGL02455:Or5w17	APN	2	87,583,927 (GRCm39)	missense	possibly damaging	0.95
IGL02698:Or5w17	APN	2	87,584,188 (GRCm39)	nonsense	probably null
PIT4480001:Or5w17	UTSW	2	87,584,127 (GRCm39)	missense	possibly damaging	0.95
R0543:Or5w17	UTSW	2	87,583,994 (GRCm39)	missense	probably damaging	1.00
R1542:Or5w17	UTSW	2	87,583,662 (GRCm39)	missense	probably damaging	0.99
R1750:Or5w17	UTSW	2	87,583,530 (GRCm39)	missense	probably damaging	0.97
R1844:Or5w17	UTSW	2	87,584,334 (GRCm39)	start codon destroyed	probably null	1.00
R2248:Or5w17	UTSW	2	87,584,287 (GRCm39)	missense	probably null	0.05
R4064:Or5w17	UTSW	2	87,584,133 (GRCm39)	missense	probably damaging	1.00
R5193:Or5w17	UTSW	2	87,583,448 (GRCm39)	missense	possibly damaging	0.59
R5861:Or5w17	UTSW	2	87,583,922 (GRCm39)	missense	probably benign	0.01
R6197:Or5w17	UTSW	2	87,583,696 (GRCm39)	missense	probably benign	0.15
R6481:Or5w17	UTSW	2	87,583,812 (GRCm39)	missense	probably damaging	1.00
R6857:Or5w17	UTSW	2	87,583,831 (GRCm39)	missense	probably damaging	1.00
R6904:Or5w17	UTSW	2	87,584,223 (GRCm39)	missense	probably benign	0.00
R6962:Or5w17	UTSW	2	87,584,071 (GRCm39)	missense	probably benign
R7014:Or5w17	UTSW	2	87,584,215 (GRCm39)	missense	probably benign	0.00
R8229:Or5w17	UTSW	2	87,583,408 (GRCm39)	missense	probably benign	0.00
R8723:Or5w17	UTSW	2	87,583,501 (GRCm39)	missense	possibly damaging	0.69
R8883:Or5w17	UTSW	2	87,583,838 (GRCm39)	missense	probably damaging	1.00
R9129:Or5w17	UTSW	2	87,584,048 (GRCm39)	missense	probably benign	0.00
R9587:Or5w17	UTSW	2	87,584,184 (GRCm39)	missense	possibly damaging	0.79
R9665:Or5w17	UTSW	2	87,583,671 (GRCm39)	missense	probably damaging	0.97
T0722:Or5w17	UTSW	2	87,583,467 (GRCm39)	missense	probably damaging	1.00
Z1177:Or5w17	UTSW	2	87,583,534 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- AGGTTTTGCAAGGTCTCTTTCAC -3'
(R):5'- CAGATGCTTTGATCCACAGAAC -3'

Sequencing Primer
(F):5'- CATCCTTGTATCTCAGGCTGTAAATC -3'
(R):5'- GATGCTTTGATCCACAGAACATTAAC -3'

Posted On

2017-10-10