Incidental Mutation 'R6151:Mknk2'
ID 489273
Institutional Source Beutler Lab
Gene Symbol Mknk2
Ensembl Gene ENSMUSG00000020190
Gene Name MAP kinase-interacting serine/threonine kinase 2
Synonyms Mnk2
MMRRC Submission 044298-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6151 (G1)
Quality Score 225.009
Status Not validated
Chromosome 10
Chromosomal Location 80665327-80678112 bp(-) (GRCm38)
Type of Mutation splice site (2921 bp from exon)
DNA Base Change (assembly) T to C at 80669025 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000143655 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000003433] [ENSMUST00000197276] [ENSMUST00000198819] [ENSMUST00000199949] [ENSMUST00000200082]
AlphaFold Q8CDB0
Predicted Effect probably benign
Transcript: ENSMUST00000003433
AA Change: I177V

PolyPhen 2 Score 0.325 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000003433
Gene: ENSMUSG00000020190
AA Change: I177V

low complexity region 13 23 N/A INTRINSIC
S_TKc 36 321 7.09e-88 SMART
Predicted Effect probably null
Transcript: ENSMUST00000197276
SMART Domains Protein: ENSMUSP00000143679
Gene: ENSMUSG00000020190

SCOP:d1koba_ 52 118 3e-11 SMART
PDB:2AC3|A 59 118 3e-32 PDB
Blast:S_TKc 71 118 1e-24 BLAST
Predicted Effect probably null
Transcript: ENSMUST00000198819
Predicted Effect probably null
Transcript: ENSMUST00000199949
Predicted Effect probably damaging
Transcript: ENSMUST00000200082
AA Change: I224V

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000143508
Gene: ENSMUSG00000020190
AA Change: I224V

low complexity region 60 70 N/A INTRINSIC
S_TKc 83 368 7.09e-88 SMART
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.4%
Validation Efficiency
MGI Phenotype FUNCTION: The protein encoded by this gene is a serine/threonine-protein kinase, which is targeted by both the extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways. This enzyme targets several substrates including eukaryotic translation initiation factor 4E and mammalian target of rapamycin, which are negatively regulated by its phosphorylation. Null mutant mice do not exhibit developmental or reproductive defects. However, mice null for both this protein and mitogen-activated protein kinase-interacting serine/threonine protein kinase 1 have delayed tumor development in phosphatase and tensin homolog mutant mice, indicating an oncogenic function for this gene in tumor development. [provided by RefSeq, Oct 2014]
PHENOTYPE: Homozygous null mice are viable and fertile with no gross abnormalities. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 99 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2410141K09Rik A T 13: 66,431,681 (GRCm38) C248S probably damaging Het
3110009E18Rik A G 1: 120,171,486 (GRCm38) probably null Het
4930550C14Rik G T 9: 53,414,383 (GRCm38) R73S probably damaging Het
Abca1 A G 4: 53,085,261 (GRCm38) V517A probably benign Het
Adck5 A T 15: 76,594,687 (GRCm38) K370N possibly damaging Het
Akap6 A C 12: 53,025,792 (GRCm38) D981A probably damaging Het
Apbb1 G A 7: 105,574,252 (GRCm38) R51* probably null Het
Arhgap23 A G 11: 97,500,412 (GRCm38) M1252V probably benign Het
Arhgef1 A G 7: 24,917,942 (GRCm38) T354A probably benign Het
Arid1a G T 4: 133,684,976 (GRCm38) Q1636K unknown Het
Asmt C T X: 170,676,467 (GRCm38) A237V possibly damaging Het
Brat1 T A 5: 140,705,961 (GRCm38) C43S probably benign Het
Cd36 T C 5: 17,795,595 (GRCm38) Y370C probably damaging Het
Ceacam12 A T 7: 18,069,105 (GRCm38) L145F probably benign Het
Col24a1 C T 3: 145,314,054 (GRCm38) T62I probably damaging Het
Col6a3 G T 1: 90,813,753 (GRCm38) N652K possibly damaging Het
Dcbld1 T C 10: 52,304,660 (GRCm38) L140P probably damaging Het
Dhrs13 G A 11: 78,036,982 (GRCm38) C218Y probably damaging Het
Diaph1 T G 18: 37,853,353 (GRCm38) E1158A probably damaging Het
Emp2 A C 16: 10,292,281 (GRCm38) F20C probably damaging Het
Enpep T C 3: 129,332,418 (GRCm38) I22V possibly damaging Het
Epha2 A G 4: 141,318,480 (GRCm38) probably null Het
Eprs G T 1: 185,407,754 (GRCm38) probably null Het
Etl4 T A 2: 20,713,360 (GRCm38) I304N probably damaging Het
Exoc3l2 C A 7: 19,491,745 (GRCm38) S89* probably null Het
F5 A T 1: 164,181,635 (GRCm38) I325F probably damaging Het
F5 G A 1: 164,190,187 (GRCm38) C611Y probably damaging Het
Fam133b A T 5: 3,559,133 (GRCm38) S116C probably null Het
Fam13b A T 18: 34,494,277 (GRCm38) D190E probably damaging Het
Fam169a T G 13: 97,093,630 (GRCm38) Y58D probably damaging Het
Far1 G A 7: 113,561,396 (GRCm38) R383H possibly damaging Het
Fnbp1l T C 3: 122,570,930 (GRCm38) K52R possibly damaging Het
Foxj3 C A 4: 119,623,271 (GRCm38) Q469K unknown Het
Frs3 A T 17: 47,689,088 (GRCm38) probably benign Het
Gdpd3 A G 7: 126,775,502 (GRCm38) S290G probably benign Het
Gm6768 T G 12: 119,261,106 (GRCm38) noncoding transcript Het
Gmip T A 8: 69,817,085 (GRCm38) L610Q probably damaging Het
Gprc5c T A 11: 114,864,025 (GRCm38) I176N probably damaging Het
Grm3 A G 5: 9,511,556 (GRCm38) F765L probably damaging Het
Hhat A T 1: 192,759,757 (GRCm38) L2Q probably damaging Het
Hrasls5 C T 19: 7,619,291 (GRCm38) P148S probably damaging Het
Hspbap1 T C 16: 35,817,222 (GRCm38) S214P probably damaging Het
Ighv1-62-3 C A 12: 115,461,289 (GRCm38) V21F probably damaging Het
Kcnj15 A T 16: 95,295,668 (GRCm38) K50* probably null Het
Kidins220 T C 12: 25,056,909 (GRCm38) S1454P possibly damaging Het
Kl T A 5: 150,988,853 (GRCm38) M689K possibly damaging Het
Klhl23 T G 2: 69,824,854 (GRCm38) L356R probably damaging Het
Kndc1 A T 7: 139,921,213 (GRCm38) D806V probably benign Het
Krt26 T C 11: 99,337,489 (GRCm38) E139G probably benign Het
Lefty1 T C 1: 180,935,116 (GRCm38) F3L unknown Het
Madd A C 2: 91,165,457 (GRCm38) Y853* probably null Het
Map1a T A 2: 121,289,823 (GRCm38) D63E probably benign Het
Mdn1 T A 4: 32,684,735 (GRCm38) V815E probably damaging Het
Mettl3 A G 14: 52,295,020 (GRCm38) Y569H probably damaging Het
Mpp3 C A 11: 102,008,566 (GRCm38) R376S probably benign Het
Nup160 T A 2: 90,690,105 (GRCm38) Y293* probably null Het
Nupl1 A T 14: 60,244,616 (GRCm38) F100I possibly damaging Het
Nxpe2 A C 9: 48,326,191 (GRCm38) L255V probably benign Het
Olfr1423 T C 19: 12,036,736 (GRCm38) E2G probably benign Het
Olfr167 A T 16: 19,515,531 (GRCm38) L35Q probably damaging Het
Olfr390 T A 11: 73,787,695 (GRCm38) Y252* probably null Het
Olfr479 A G 7: 108,055,899 (GRCm38) I306V probably benign Het
Padi3 A T 4: 140,796,394 (GRCm38) D248E probably damaging Het
Paxip1 G T 5: 27,761,618 (GRCm38) H637N probably damaging Het
Pde4b T A 4: 102,601,551 (GRCm38) M296K probably damaging Het
Pkd1 A G 17: 24,575,606 (GRCm38) H2089R probably benign Het
Prpf40a T C 2: 53,157,915 (GRCm38) M197V probably benign Het
Rhebl1 T C 15: 98,878,279 (GRCm38) I165V probably benign Het
Rock1 A T 18: 10,106,426 (GRCm38) V481E possibly damaging Het
Rtca T A 3: 116,507,827 (GRCm38) T24S probably benign Het
Serpina3c T A 12: 104,152,068 (GRCm38) I4F possibly damaging Het
Serpina3j C A 12: 104,317,390 (GRCm38) A249E possibly damaging Het
Slc38a9 A G 13: 112,689,376 (GRCm38) N116S probably damaging Het
Slco2b1 C T 7: 99,690,563 (GRCm38) V58I possibly damaging Het
Slfn8 T A 11: 83,017,321 (GRCm38) Y132F probably damaging Het
Smg6 T G 11: 75,156,207 (GRCm38) I1242S probably damaging Het
Tap2 G T 17: 34,212,047 (GRCm38) V374L probably benign Het
Tbc1d10b G A 7: 127,207,996 (GRCm38) T123M probably damaging Het
Tenm2 A C 11: 36,008,783 (GRCm38) V2516G probably damaging Het
Tenm3 C T 8: 48,395,573 (GRCm38) R12Q probably damaging Het
Tmem130 T A 5: 144,737,851 (GRCm38) M355L probably benign Het
Tns4 C T 11: 99,075,550 (GRCm38) S433N probably benign Het
Traip G T 9: 107,970,619 (GRCm38) probably null Het
Trmo G A 4: 46,389,390 (GRCm38) R2C probably damaging Het
Ttn A T 2: 76,944,160 (GRCm38) I2134N probably damaging Het
Uba1y A G Y: 825,984 (GRCm38) D380G probably benign Het
Ube2t T A 1: 134,967,960 (GRCm38) probably null Het
Ugdh A T 5: 65,417,581 (GRCm38) Y367* probably null Het
Usp45 A C 4: 21,810,797 (GRCm38) D331A probably damaging Het
Vmn1r84 G T 7: 12,361,914 (GRCm38) T284K possibly damaging Het
Vmn1r91 T A 7: 20,101,435 (GRCm38) F93Y probably benign Het
Vmn2r77 T A 7: 86,801,670 (GRCm38) Y255N probably benign Het
Vmn2r96 A T 17: 18,583,959 (GRCm38) Q490H probably benign Het
Vwa2 A G 19: 56,903,465 (GRCm38) probably null Het
Vwf A G 6: 125,657,065 (GRCm38) K169E unknown Het
Zc3h7b G C 15: 81,778,710 (GRCm38) probably null Het
Zfp458 T A 13: 67,257,598 (GRCm38) H259L possibly damaging Het
Zfp647 G A 15: 76,912,085 (GRCm38) P125L probably damaging Het
Zfp804b A G 5: 6,769,910 (GRCm38) V1051A probably benign Het
Other mutations in Mknk2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01468:Mknk2 APN 10 80,667,664 (GRCm38) splice site probably benign
IGL02471:Mknk2 APN 10 80,668,121 (GRCm38) missense probably damaging 0.99
IGL02643:Mknk2 APN 10 80,668,601 (GRCm38) missense probably damaging 1.00
H8562:Mknk2 UTSW 10 80,668,934 (GRCm38) splice site probably benign
IGL03052:Mknk2 UTSW 10 80,669,662 (GRCm38) missense probably benign 0.12
R0645:Mknk2 UTSW 10 80,671,908 (GRCm38) splice site probably null
R2061:Mknk2 UTSW 10 80,671,557 (GRCm38) critical splice donor site probably null
R2105:Mknk2 UTSW 10 80,668,601 (GRCm38) missense possibly damaging 0.90
R2167:Mknk2 UTSW 10 80,668,701 (GRCm38) missense probably damaging 1.00
R3847:Mknk2 UTSW 10 80,667,975 (GRCm38) nonsense probably null
R4649:Mknk2 UTSW 10 80,669,339 (GRCm38) missense probably damaging 1.00
R5062:Mknk2 UTSW 10 80,671,769 (GRCm38) missense probably damaging 1.00
R5358:Mknk2 UTSW 10 80,671,763 (GRCm38) missense probably benign 0.19
R5433:Mknk2 UTSW 10 80,667,225 (GRCm38) missense probably benign 0.00
R5518:Mknk2 UTSW 10 80,668,641 (GRCm38) missense possibly damaging 0.92
R5813:Mknk2 UTSW 10 80,675,862 (GRCm38) missense probably benign 0.34
R6060:Mknk2 UTSW 10 80,671,634 (GRCm38) missense probably benign 0.00
R6366:Mknk2 UTSW 10 80,671,933 (GRCm38) missense probably damaging 0.99
R7640:Mknk2 UTSW 10 80,668,566 (GRCm38) missense probably benign 0.00
R7827:Mknk2 UTSW 10 80,667,187 (GRCm38) missense probably benign 0.03
R7943:Mknk2 UTSW 10 80,675,867 (GRCm38) missense probably benign 0.00
R8075:Mknk2 UTSW 10 80,672,148 (GRCm38) intron probably benign
R9114:Mknk2 UTSW 10 80,668,989 (GRCm38) missense probably damaging 1.00
R9140:Mknk2 UTSW 10 80,671,593 (GRCm38) missense probably benign 0.22
R9451:Mknk2 UTSW 10 80,669,662 (GRCm38) missense probably benign 0.12
R9506:Mknk2 UTSW 10 80,668,084 (GRCm38) missense probably benign 0.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2017-10-10