Mutagenetix > Incidental Mutation

Incidental Mutation 'R6158:Vmn2r63'

489704

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

044305-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R6158 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

42903251-42933789 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 42933680 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 37 (D37V)

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803]

AlphaFold

E9Q0K5

Predicted Effect

probably damaging
Transcript: ENSMUST00000163803
AA Change: D37V

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751
AA Change: D37V

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.5%
20x: 92.6%

Validation Efficiency

98% (65/66)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	T	C	12: 71,170,636	V793A	possibly damaging	Het
2900092C05Rik	A	G	7: 12,512,672	T32A	probably benign	Het
Adprhl1	C	T	8: 13,224,977	V594M	possibly damaging	Het
Ano3	A	T	2: 110,665,875	Y845N	probably damaging	Het
Arhgap24	A	T	5: 102,892,912	I575L	probably benign	Het
Aurka	A	C	2: 172,363,596		probably null	Het
C1qtnf5	A	T	9: 44,108,970		probably benign	Het
Cacnb2	A	G	2: 14,985,601	D454G	possibly damaging	Het
Ccdc58	T	A	16: 36,089,929	V118D	probably damaging	Het
Chchd5	T	C	2: 129,130,517	L87P	probably damaging	Het
Col7a1	G	T	9: 108,964,603	R1377L	unknown	Het
Cpne8	A	C	15: 90,571,988	S191A	probably damaging	Het
Dhx30	A	T	9: 110,087,030	I671N	probably damaging	Het
Dnah12	A	T	14: 26,773,685	K1423N	possibly damaging	Het
Dnm3	T	C	1: 162,320,987	M272V	probably damaging	Het
Fat4	A	T	3: 38,983,262	S3688C	possibly damaging	Het
Frmpd1	A	T	4: 45,285,401	L1407F	probably damaging	Het
Fry	T	C	5: 150,454,572	S410P	probably damaging	Het
Gm11565	T	A	11: 99,914,918	C45*	probably null	Het
Gngt1	A	T	6: 3,994,311	R30*	probably null	Het
Htt	T	C	5: 34,907,086	I2943T	possibly damaging	Het
Kl	T	A	5: 150,988,853	M689K	possibly damaging	Het
Lmo7	T	A	14: 101,900,137	D247E	probably benign	Het
Mastl	G	T	2: 23,132,772	N646K	possibly damaging	Het
Mei4	G	T	9: 81,927,576	L237F	probably damaging	Het
Mettl27	C	T	5: 134,940,576	P170S	possibly damaging	Het
Mgam	T	A	6: 40,757,714	I896K	probably damaging	Het
Moxd1	T	A	10: 24,284,777	C443S	probably damaging	Het
Myo18b	A	T	5: 112,874,172	N451K	probably benign	Het
Myo7b	T	C	18: 31,988,549	I768V	probably benign	Het
Nos1	A	C	5: 117,867,574	I120L	probably benign	Het
Nsd1	T	C	13: 55,245,621	V345A	probably benign	Het
Olfr1095	A	G	2: 86,851,515	L61P	possibly damaging	Het
Olfr1205	T	C	2: 88,831,146	F10L	probably damaging	Het
Olfr150	C	T	9: 39,737,076	T87I	probably benign	Het
Olfr170	C	A	16: 19,605,925	V248F	probably damaging	Het
Olfr344	A	T	2: 36,569,116	T173S	probably benign	Het
Pdzph1	T	G	17: 58,973,627	Q553H	probably damaging	Het
Piwil1	T	A	5: 128,747,876	L546*	probably null	Het
Pla2g4f	T	C	2: 120,301,071	T724A	probably benign	Het
Ralgapa2	A	T	2: 146,424,676	M660K	possibly damaging	Het
Rgsl1	T	C	1: 153,804,021	D103G	possibly damaging	Het
Rnf186	A	G	4: 138,967,254	D35G	probably damaging	Het
Rock2	A	G	12: 16,954,918	D424G	probably benign	Het
Scg2	A	T	1: 79,435,400	D495E	probably damaging	Het
Slc39a2	G	A	14: 51,894,224		probably null	Het
Snrnp48	T	A	13: 38,210,236	Y100*	probably null	Het
Spaca1	A	G	4: 34,029,176	M99T	probably damaging	Het
Specc1	T	G	11: 62,118,124	F235L	probably damaging	Het
St13	A	T	15: 81,399,601		probably null	Het
Swap70	T	A	7: 110,270,023	M341K	probably damaging	Het
Synj2	A	G	17: 5,986,212	D67G	probably benign	Het
Tmem135	T	A	7: 89,156,444	I251F	probably benign	Het
Tmem87a	A	T	2: 120,360,103		probably null	Het
Tom1l2	C	T	11: 60,232,927	D128N	probably damaging	Het
Tpx2	C	A	2: 152,873,104	H82N	probably benign	Het
Trip12	A	T	1: 84,761,012	C738S	possibly damaging	Het
Ttyh1	A	G	7: 4,125,562	T153A	probably benign	Het
Utrn	C	T	10: 12,690,822	G1199S	probably benign	Het
Vmn1r8	T	A	6: 57,036,289	N108K	probably benign	Het
Vwce	C	T	19: 10,644,221	R206C	possibly damaging	Het
Wrn	G	T	8: 33,319,172	F265L	probably damaging	Het
Zfp472	T	A	17: 32,978,389	C479*	probably null	Het
Zfp831	A	T	2: 174,643,858	T109S	possibly damaging	Het
Znfx1	G	T	2: 167,056,726	Q93K	probably benign	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42904119	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42903364	missense	probably benign
IGL02203:Vmn2r63	APN	7	42904008	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42926850	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42903454	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42929192	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42927944	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42903985	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42927090	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42903275	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42903618	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42933705	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42928528	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42928010	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42928035	missense	probably damaging	1.00
R1349:Vmn2r63	UTSW	7	42929218	missense	possibly damaging	0.95
R1372:Vmn2r63	UTSW	7	42929218	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42927915	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42928591	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42904126	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42928211	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42928319	missense	probably benign
R1698:Vmn2r63	UTSW	7	42933614	missense	probably benign
R1753:Vmn2r63	UTSW	7	42928245	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42926873	missense	probably damaging	0.99
R2175:Vmn2r63	UTSW	7	42933580	critical splice donor site	probably null
R2261:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R2263:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3427:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42903405	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4323:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42928113	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42933826	splice site	probably null
R4649:Vmn2r63	UTSW	7	42903690	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42903690	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42926890	missense	probably benign	0.34
R4933:Vmn2r63	UTSW	7	42903978	missense	probably damaging	1.00
R5198:Vmn2r63	UTSW	7	42903745	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42928277	missense	probably benign
R5400:Vmn2r63	UTSW	7	42928211	missense	probably benign	0.32
R6185:Vmn2r63	UTSW	7	42929011	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42928635	splice site	probably null
R6362:Vmn2r63	UTSW	7	42903297	missense	probably benign
R6706:Vmn2r63	UTSW	7	42928577	missense	probably damaging	1.00
R6764:Vmn2r63	UTSW	7	42903271	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42928535	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42933590	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42926967	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42926982	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42925269	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42927042	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42928129	missense	possibly damaging	0.46
R7918:Vmn2r63	UTSW	7	42903531	missense	probably damaging	1.00
R8826:Vmn2r63	UTSW	7	42927017	missense	probably benign	0.38
R8973:Vmn2r63	UTSW	7	42928495	missense	probably benign	0.19
R9024:Vmn2r63	UTSW	7	42928450	missense	probably benign	0.14
R9154:Vmn2r63	UTSW	7	42926989	missense	probably damaging	0.96
R9731:Vmn2r63	UTSW	7	42903937	missense	probably benign	0.32
Z1088:Vmn2r63	UTSW	7	42928559	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- CTTCAGAGATTTGAGAGTGGAAAAC -3'
(R):5'- CTGCCCTCATTAGCAAAGTTAG -3'

Sequencing Primer
(F):5'- TTGAGAGTGGAAAACAGTTATTGTG -3'
(R):5'- CTTGACATCAATATATACCTGCTGG -3'

Posted On

2017-10-10