Mutagenetix > Incidental Mutation

Incidental Mutation 'R0529:Vmn2r12'

49043

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

038721-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R0529 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109085849-109097864 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109092848 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 133 (V133A)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

probably benign
Transcript: ENSMUST00000095922
AA Change: V133A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: V133A

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.5%
20x: 93.3%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	G	A	17: 8,992,396	V126I	probably benign	Het
Aasdh	A	C	5: 76,876,267	Y179*	probably null	Het
Afp	A	G	5: 90,504,395	Y415C	probably damaging	Het
Aldh5a1	G	T	13: 24,913,873	T393K	probably benign	Het
Arhgef26	T	C	3: 62,339,725	S77P	probably benign	Het
Axl	A	G	7: 25,787,287		probably benign	Het
Card10	A	G	15: 78,780,475		probably null	Het
Ccdc71l	G	A	12: 32,379,252	S90N	probably damaging	Het
Cebpa	A	T	7: 35,120,199	T261S	probably benign	Het
Cnmd	T	C	14: 79,642,041	E219G	probably benign	Het
Cntln	T	A	4: 85,067,825	L1010H	probably damaging	Het
Cul9	A	G	17: 46,520,468		probably benign	Het
Cyld	A	G	8: 88,729,759	E479G	probably benign	Het
Dmp1	A	G	5: 104,212,226	E256G	probably benign	Het
Dnmt1	T	C	9: 20,911,550	D1140G	probably damaging	Het
Drd2	A	C	9: 49,407,074	M439L	probably benign	Het
Drd3	G	A	16: 43,822,714	V438M	probably damaging	Het
Dyrk3	A	G	1: 131,130,121	I70T	probably benign	Het
Fam46c	A	G	3: 100,472,370	Y357H	probably benign	Het
Fbxo38	T	C	18: 62,505,986	K1082E	probably damaging	Het
Fbxw10	A	C	11: 62,859,845	D428A	probably damaging	Het
Fmn1	T	A	2: 113,707,853		probably benign	Het
Fmnl2	A	T	2: 53,042,365	I119F	probably damaging	Het
Frmd4a	A	G	2: 4,606,023	T995A	probably damaging	Het
Gda	T	A	19: 21,425,537	I82F	probably damaging	Het
Gpatch4	T	A	3: 88,051,276	H22Q	probably damaging	Het
Gpr55	A	G	1: 85,941,503	F119L	probably benign	Het
Gtf2i	A	T	5: 134,261,869	L425*	probably null	Het
Knstrn	T	A	2: 118,830,980		probably benign	Het
Lipo2	T	A	19: 33,746,935	I144L	probably benign	Het
Lrp1	T	C	10: 127,541,594		probably null	Het
Mtmr14	T	C	6: 113,266,252		probably benign	Het
Nsmce4a	A	T	7: 130,533,806	S345R	probably benign	Het
Oacyl	T	A	18: 65,742,219	V385D	probably damaging	Het
Olfr421-ps1	G	A	1: 174,152,130	A205T	probably benign	Het
Olfr917	A	T	9: 38,665,512	C111S	probably benign	Het
Phlpp2	T	C	8: 109,876,971	S55P	probably benign	Het
Pkhd1l1	T	A	15: 44,526,754	V1422E	possibly damaging	Het
Plcd3	T	G	11: 103,080,187	H181P	probably benign	Het
Psmc5	G	A	11: 106,261,164		probably null	Het
Psmd11	T	C	11: 80,470,689		probably benign	Het
Rab39	T	C	9: 53,686,716	Y83C	probably damaging	Het
Ric8a	A	G	7: 140,860,893	E93G	probably damaging	Het
Rtp3	T	C	9: 110,987,084	E133G	possibly damaging	Het
Serpina1e	A	C	12: 103,949,104	L281R	probably damaging	Het
Slc35e1	T	C	8: 72,492,571		probably benign	Het
Tmem63a	A	T	1: 180,961,094	E332V	probably benign	Het
Tnk1	T	C	11: 69,855,164	T312A	probably damaging	Het
Traf3ip3	G	T	1: 193,194,811		probably benign	Het
Trappc11	A	G	8: 47,526,979	V174A	possibly damaging	Het
Vmn1r174	G	A	7: 23,754,197	R96H	probably benign	Het
Vmn1r7	T	A	6: 57,024,465	Y270F	possibly damaging	Het
Vmn2r18	T	A	5: 151,562,523	E502V	probably damaging	Het
Wipf3	C	A	6: 54,485,363	P186Q	probably damaging	Het
Yipf5	A	T	18: 40,212,162	M55K	probably benign	Het
Zbtb7a	G	A	10: 81,143,986	V5M	probably damaging	Het
Zfy1	G	T	Y: 726,040	S575Y	probably damaging	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109097675	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109086259	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109091850	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109093027	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109086564	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109092159	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109086477	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109085992	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109090485	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109092070	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109092899	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109091889	nonsense	probably null
R0715:Vmn2r12	UTSW	5	109090507	missense	probably benign	0.10
R0742:Vmn2r12	UTSW	5	109086415	missense	possibly damaging	0.55
R0894:Vmn2r12	UTSW	5	109087850	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109092854	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109092854	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109091897	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109086586	missense	probably benign	0.00
R1388:Vmn2r12	UTSW	5	109092974	missense	possibly damaging	0.56
R1549:Vmn2r12	UTSW	5	109092830	missense	probably benign	0.06
R1766:Vmn2r12	UTSW	5	109092044	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109091728	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109092076	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109091474	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109091531	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109091531	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109090504	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109092192	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109092192	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109091546	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109086435	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109086513	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109092986	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109091678	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109091506	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109090395	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109091818	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109086617	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109092800	missense	probably benign	0.06
R5753:Vmn2r12	UTSW	5	109091804	missense	probably damaging	1.00
R5797:Vmn2r12	UTSW	5	109085870	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109092897	missense	probably benign
R6162:Vmn2r12	UTSW	5	109086564	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109086000	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109092905	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109097789	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109086247	missense	possibly damaging	0.74
R7341:Vmn2r12	UTSW	5	109091945	missense	possibly damaging	0.95
R7383:Vmn2r12	UTSW	5	109092818	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109091749	missense	probably damaging	1.00
R7749:Vmn2r12	UTSW	5	109086054	missense	probably damaging	0.99
R7861:Vmn2r12	UTSW	5	109087963	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109086441	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109091881	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109090483	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109086208	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109092086	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109086656	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109086167	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109086019	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109093044	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109086337	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109086586	missense	probably benign	0.00
R9375:Vmn2r12	UTSW	5	109086120	missense	probably damaging	1.00
R9524:Vmn2r12	UTSW	5	109091957	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109091456	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109092780	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109091437	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTGTTAACTTgggctatcgagctgg -3'
(R):5'- TGGCAGCACTCTGACTCTCCAC -3'

Sequencing Primer
(F):5'- tcctaaattcaattcccaataaccac -3'
(R):5'- CTAGTTCAAGGTTAGCATTCAGAG -3'

Posted On

2013-06-12