Mutagenetix > Incidental Mutation

Incidental Mutation 'R0532:Zmym4'

49220

Institutional Source

Beutler Lab

Gene Symbol

Zmym4

Ensembl Gene

ENSMUSG00000042446

Gene Name

zinc finger, MYM-type 4

Synonyms

Zfp262, 6330503C17Rik

MMRRC Submission

038724-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.586) question?

Stock #

R0532 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

126755732-126861928 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 126792194 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 596 (Q596*)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106108]

AlphaFold

A2A791

Predicted Effect

probably null
Transcript: ENSMUST00000106108
AA Change: Q937*

SMART Domains

Protein: ENSMUSP00000101714
Gene: ENSMUSG00000042446
AA Change: Q937*

Domain	Start	End	E-Value	Type
TRASH	341	377	6.53e-4	SMART
TRASH	389	429	7.22e-6	SMART
TRASH	441	479	1.77e0	SMART
TRASH	486	525	4.95e-4	SMART
TRASH	531	569	1.05e-2	SMART
TRASH	579	615	2.82e1	SMART
low complexity region	640	649	N/A	INTRINSIC
TRASH	687	723	8.49e-3	SMART
TRASH	729	764	1.14e-3	SMART
TRASH	772	810	4.48e-2	SMART
TRASH	816	851	2.06e-1	SMART
low complexity region	974	993	N/A	INTRINSIC
low complexity region	1002	1021	N/A	INTRINSIC
Pfam:DUF3504	1357	1527	1.7e-68	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000135003
AA Change: Q596*

SMART Domains

Protein: ENSMUSP00000120813
Gene: ENSMUSG00000042446
AA Change: Q596*

Domain	Start	End	E-Value	Type
TRASH	90	126	6.53e-4	SMART
TRASH	138	178	7.22e-6	SMART
TRASH	190	228	1.77e0	SMART
TRASH	235	274	3.05e-4	SMART
low complexity region	300	309	N/A	INTRINSIC
TRASH	347	383	8.49e-3	SMART
TRASH	389	424	1.14e-3	SMART
TRASH	432	470	4.48e-2	SMART
TRASH	476	511	2.06e-1	SMART
low complexity region	634	653	N/A	INTRINSIC
low complexity region	662	681	N/A	INTRINSIC
Pfam:DUF3504	1017	1187	1.5e-68	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.5%
20x: 93.2%

Validation Efficiency

100% (90/90)

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921524L21Rik	A	T	18: 6,638,618 (GRCm39)	E339V	possibly damaging	Het
9230009I02Rik	A	T	11: 50,982,405 (GRCm39)		noncoding transcript	Het
9230112D13Rik	A	T	14: 34,234,054 (GRCm39)	I79K	unknown	Het
Adam25	C	T	8: 41,208,987 (GRCm39)	T751I	probably benign	Het
Adgrv1	C	A	13: 81,727,015 (GRCm39)	V446L	probably damaging	Het
Afap1	C	A	5: 36,125,944 (GRCm39)	A313D	possibly damaging	Het
Akap6	A	G	12: 52,934,766 (GRCm39)	T753A	probably benign	Het
Aldh16a1	G	T	7: 44,792,262 (GRCm39)	T730N	probably damaging	Het
Amfr	A	T	8: 94,725,736 (GRCm39)	M215K	probably damaging	Het
Apob	A	G	12: 8,066,188 (GRCm39)	R4386G	possibly damaging	Het
Arhgap45	A	T	10: 79,857,917 (GRCm39)	M217L	possibly damaging	Het
Baiap2l2	C	T	15: 79,168,276 (GRCm39)	E49K	possibly damaging	Het
Baz1a	C	T	12: 54,981,605 (GRCm39)	E350K	possibly damaging	Het
Bbx	A	T	16: 50,086,647 (GRCm39)	V83D	probably damaging	Het
Btaf1	T	C	19: 36,928,586 (GRCm39)		probably benign	Het
Cacna2d1	G	A	5: 16,567,271 (GRCm39)	E942K	probably benign	Het
Cad	T	C	5: 31,219,531 (GRCm39)		probably benign	Het
Ccdc96	A	G	5: 36,643,710 (GRCm39)	K572R	probably benign	Het
Cdc5l	G	A	17: 45,726,610 (GRCm39)	R321W	probably damaging	Het
Cep164	G	A	9: 45,721,124 (GRCm39)	R93*	probably null	Het
Cir1	A	G	2: 73,140,799 (GRCm39)		probably null	Het
Crocc	A	G	4: 140,757,558 (GRCm39)	S912P	possibly damaging	Het
Cwf19l2	T	C	9: 3,431,057 (GRCm39)	L463P	probably benign	Het
Cyp3a59	C	T	5: 146,033,463 (GRCm39)	Q200*	probably null	Het
Cyp4b1	G	T	4: 115,484,073 (GRCm39)	P303T	probably damaging	Het
Dcbld1	C	A	10: 52,193,173 (GRCm39)	T306K	probably benign	Het
Dgat2	A	G	7: 98,818,988 (GRCm39)	V56A	possibly damaging	Het
Dnajc16	A	C	4: 141,516,320 (GRCm39)	L16R	probably damaging	Het
Dnmt1	A	C	9: 20,829,852 (GRCm39)		probably benign	Het
Dus3l	A	G	17: 57,076,308 (GRCm39)	I528V	probably damaging	Het
Egflam	T	C	15: 7,263,718 (GRCm39)	D744G	probably benign	Het
Epb41	A	C	4: 131,706,106 (GRCm39)		probably benign	Het
Eri2	C	T	7: 119,385,206 (GRCm39)	V432I	probably benign	Het
Esyt2	A	G	12: 116,320,818 (GRCm39)		probably benign	Het
Extl3	A	T	14: 65,315,122 (GRCm39)	M20K	probably benign	Het
Fam32a	A	G	8: 72,976,063 (GRCm39)	Y103C	probably damaging	Het
Fat4	T	C	3: 39,035,870 (GRCm39)	V3174A	probably benign	Het
Fbxo40	T	A	16: 36,789,984 (GRCm39)	E375D	possibly damaging	Het
Frrs1	A	G	3: 116,676,813 (GRCm39)	T182A	probably benign	Het
Fry	A	G	5: 150,357,172 (GRCm39)		probably benign	Het
Fry	T	C	5: 150,402,226 (GRCm39)		probably benign	Het
Fsip2	A	G	2: 82,808,129 (GRCm39)	I1483V	probably benign	Het
Glra3	T	A	8: 56,578,111 (GRCm39)	D389E	probably benign	Het
Gpr3	A	T	4: 132,937,796 (GRCm39)	I292N	probably damaging	Het
Grina	T	C	15: 76,133,045 (GRCm39)	M230T	probably damaging	Het
Igkv11-125	G	A	6: 67,890,603 (GRCm39)	W16*	probably null	Het
Il18r1	T	C	1: 40,514,061 (GRCm39)	V89A	probably damaging	Het
Ino80	T	C	2: 119,212,464 (GRCm39)	E1286G	possibly damaging	Het
Iqch	G	A	9: 63,415,514 (GRCm39)		probably benign	Het
Itpr2	G	T	6: 146,013,898 (GRCm39)	Q2666K	probably damaging	Het
Kcnh3	A	G	15: 99,130,844 (GRCm39)	D487G	probably damaging	Het
Kdm1a	A	G	4: 136,288,377 (GRCm39)	L402P	probably damaging	Het
Klhl10	T	G	11: 100,337,937 (GRCm39)		probably benign	Het
Krt39	T	C	11: 99,405,617 (GRCm39)	T428A	possibly damaging	Het
Mapk3	G	A	7: 126,362,558 (GRCm39)		probably benign	Het
Med13l	T	A	5: 118,897,188 (GRCm39)	S2089T	possibly damaging	Het
Mex3c	G	A	18: 73,723,124 (GRCm39)	D406N	possibly damaging	Het
Mki67	G	A	7: 135,299,893 (GRCm39)	R1714*	probably null	Het
Mmp9	C	A	2: 164,791,740 (GRCm39)	S211*	probably null	Het
Nat8f2	G	T	6: 85,844,784 (GRCm39)	Q193K	probably benign	Het
Omt2a	C	A	9: 78,220,187 (GRCm39)	A71S	possibly damaging	Het
Or10ak11	T	G	4: 118,686,897 (GRCm39)	T247P	probably damaging	Het
Or5ac22	T	C	16: 59,134,964 (GRCm39)	K269E	probably benign	Het
Pdgfra	G	A	5: 75,331,434 (GRCm39)	V315I	probably benign	Het
Pdgfrb	A	G	18: 61,216,337 (GRCm39)	D1065G	probably damaging	Het
Pfas	A	T	11: 68,893,455 (GRCm39)		probably benign	Het
Pramel5	T	C	4: 143,999,310 (GRCm39)	E259G	probably benign	Het
Prpf6	A	G	2: 181,264,004 (GRCm39)	Y222C	possibly damaging	Het
Rbck1	C	A	2: 152,166,250 (GRCm39)	Q229H	probably damaging	Het
Rdm1	T	A	11: 101,526,661 (GRCm39)	C278S	probably benign	Het
Sall1	T	C	8: 89,759,819 (GRCm39)	D95G	probably benign	Het
Scn1a	T	A	2: 66,148,167 (GRCm39)	D1126V	probably damaging	Het
Scn4a	C	T	11: 106,221,226 (GRCm39)	G811D	probably benign	Het
Sh2b1	A	G	7: 126,071,444 (GRCm39)	I247T	probably benign	Het
Shprh	C	A	10: 11,038,556 (GRCm39)	T437K	possibly damaging	Het
Slc13a4	A	T	6: 35,264,339 (GRCm39)		probably null	Het
Slc16a1	C	A	3: 104,560,734 (GRCm39)	Y346*	probably null	Het
Slc25a38	G	T	9: 119,949,772 (GRCm39)	A163S	probably damaging	Het
Slc6a12	G	A	6: 121,333,877 (GRCm39)	V238I	probably damaging	Het
Slc8b1	C	A	5: 120,657,736 (GRCm39)	D66E	probably damaging	Het
Snapin	A	G	3: 90,396,893 (GRCm39)	L106P	probably damaging	Het
Tas2r122	G	A	6: 132,688,791 (GRCm39)	S34F	possibly damaging	Het
Tiam2	A	G	17: 3,471,921 (GRCm39)	K521R	probably damaging	Het
Tmem81	C	G	1: 132,435,567 (GRCm39)	I124M	probably damaging	Het
Ttc3	T	C	16: 94,188,189 (GRCm39)		probably benign	Het
Uba1y	T	C	Y: 820,911 (GRCm39)	F31L	probably benign	Het
Ucp3	A	G	7: 100,131,186 (GRCm39)		probably benign	Het
Vcan	T	C	13: 89,851,891 (GRCm39)	E1023G	probably damaging	Het
Vmn2r45	A	G	7: 8,474,820 (GRCm39)	I736T	probably damaging	Het
Vps36	T	C	8: 22,708,261 (GRCm39)	F342L	probably benign	Het
Zc3hc1	A	G	6: 30,374,929 (GRCm39)		probably benign	Het

Other mutations in Zmym4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00537:Zmym4	APN	4	126,783,851 (GRCm39)	missense	probably benign
IGL00845:Zmym4	APN	4	126,794,406 (GRCm39)	missense	probably benign	0.28
IGL01122:Zmym4	APN	4	126,758,045 (GRCm39)	missense	probably damaging	1.00
IGL01374:Zmym4	APN	4	126,762,750 (GRCm39)	missense	probably damaging	1.00
IGL01564:Zmym4	APN	4	126,805,073 (GRCm39)	missense	possibly damaging	0.84
IGL02014:Zmym4	APN	4	126,794,462 (GRCm39)	missense	possibly damaging	0.67
IGL02187:Zmym4	APN	4	126,764,066 (GRCm39)	missense	probably damaging	0.97
IGL02887:Zmym4	APN	4	126,842,268 (GRCm39)	missense	probably damaging	0.96
IGL03371:Zmym4	APN	4	126,808,881 (GRCm39)	missense	possibly damaging	0.90
IGL03400:Zmym4	APN	4	126,816,920 (GRCm39)	missense	probably benign	0.12
arriba	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
foreclosed	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
Foreshortened	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
levantese	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
terminated	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
BB004:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
BB014:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R0149:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0361:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0423:Zmym4	UTSW	4	126,776,112 (GRCm39)	splice site	probably benign
R0745:Zmym4	UTSW	4	126,796,496 (GRCm39)	splice site	probably benign
R1183:Zmym4	UTSW	4	126,819,632 (GRCm39)	missense	probably damaging	0.99
R1401:Zmym4	UTSW	4	126,804,962 (GRCm39)	missense	probably benign	0.00
R1446:Zmym4	UTSW	4	126,776,275 (GRCm39)	missense	probably damaging	1.00
R1491:Zmym4	UTSW	4	126,776,105 (GRCm39)	critical splice acceptor site	probably null
R1566:Zmym4	UTSW	4	126,804,940 (GRCm39)	missense	possibly damaging	0.94
R1962:Zmym4	UTSW	4	126,796,463 (GRCm39)	missense	possibly damaging	0.47
R2398:Zmym4	UTSW	4	126,816,929 (GRCm39)	missense	probably damaging	1.00
R2930:Zmym4	UTSW	4	126,819,316 (GRCm39)	missense	probably benign	0.00
R3891:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R3892:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R4659:Zmym4	UTSW	4	126,842,221 (GRCm39)	splice site	probably null
R4702:Zmym4	UTSW	4	126,816,958 (GRCm39)	missense	probably benign	0.01
R5160:Zmym4	UTSW	4	126,763,977 (GRCm39)	missense	probably damaging	0.97
R5614:Zmym4	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
R5773:Zmym4	UTSW	4	126,799,163 (GRCm39)	missense	possibly damaging	0.52
R6450:Zmym4	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
R6741:Zmym4	UTSW	4	126,808,878 (GRCm39)	missense	possibly damaging	0.80
R7023:Zmym4	UTSW	4	126,762,593 (GRCm39)	missense	probably damaging	1.00
R7315:Zmym4	UTSW	4	126,776,385 (GRCm39)	missense	probably benign	0.09
R7468:Zmym4	UTSW	4	126,776,029 (GRCm39)	missense	probably benign	0.40
R7546:Zmym4	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
R7609:Zmym4	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
R7764:Zmym4	UTSW	4	126,819,409 (GRCm39)	missense	probably benign	0.06
R7897:Zmym4	UTSW	4	126,783,332 (GRCm39)	missense	possibly damaging	0.76
R7918:Zmym4	UTSW	4	126,804,797 (GRCm39)	critical splice acceptor site	probably null
R7927:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R8129:Zmym4	UTSW	4	126,808,956 (GRCm39)	missense	possibly damaging	0.87
R8240:Zmym4	UTSW	4	126,798,188 (GRCm39)	critical splice donor site	probably null
R8248:Zmym4	UTSW	4	126,799,162 (GRCm39)	missense	possibly damaging	0.56
R8261:Zmym4	UTSW	4	126,798,360 (GRCm39)	missense	probably damaging	1.00
R8313:Zmym4	UTSW	4	126,804,762 (GRCm39)	missense	probably benign	0.19
R8353:Zmym4	UTSW	4	126,800,905 (GRCm39)	missense	possibly damaging	0.46
R8747:Zmym4	UTSW	4	126,787,198 (GRCm39)	missense	probably damaging	1.00
R8787:Zmym4	UTSW	4	126,816,953 (GRCm39)	missense	probably benign	0.41
R8795:Zmym4	UTSW	4	126,799,819 (GRCm39)	missense	probably benign	0.35
R8948:Zmym4	UTSW	4	126,758,060 (GRCm39)	missense	probably damaging	1.00
R9218:Zmym4	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
R9233:Zmym4	UTSW	4	126,776,310 (GRCm39)	missense	probably damaging	0.99
R9286:Zmym4	UTSW	4	126,783,812 (GRCm39)	missense	probably damaging	1.00
R9468:Zmym4	UTSW	4	126,800,993 (GRCm39)	missense	probably benign	0.01
R9542:Zmym4	UTSW	4	126,799,164 (GRCm39)	missense	probably benign	0.00
R9756:Zmym4	UTSW	4	126,771,502 (GRCm39)	missense	probably damaging	1.00
R9776:Zmym4	UTSW	4	126,804,942 (GRCm39)	missense	possibly damaging	0.51
U24488:Zmym4	UTSW	4	126,819,453 (GRCm39)	missense	possibly damaging	0.69
Z1177:Zmym4	UTSW	4	126,801,005 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ACACTGGGCAATCCACGTAAGC -3'
(R):5'- TGGTTACATCCTCCAAGTCAGTCAGG -3'

Sequencing Primer
(F):5'- CACGTAAGCAGGAGACAGTACTC -3'
(R):5'- tcagaaatccgcctgcc -3'

Posted On

2013-06-12