Incidental Mutation 'R0533:Olfr681'
ID 49321
Institutional Source Beutler Lab
Gene Symbol Olfr681
Ensembl Gene ENSMUSG00000095248
Gene Name olfactory receptor 681
Synonyms MOR40-14, GA_x6K02T2PBJ9-7750163-7751110
MMRRC Submission 038725-MU
Accession Numbers
Is this an essential gene? Possibly non essential (E-score: 0.324) question?
Stock # R0533 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 105116836-105125523 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) G to A at 105122350 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Valine to Isoleucine at position 298 (V298I)
Ref Sequence ENSEMBL: ENSMUSP00000150733 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000071242] [ENSMUST00000098157] [ENSMUST00000214399] [ENSMUST00000215517] [ENSMUST00000215564] [ENSMUST00000216247]
AlphaFold Q3SXH8
Predicted Effect probably benign
Transcript: ENSMUST00000071242
SMART Domains Protein: ENSMUSP00000071223
Gene: ENSMUSG00000059768

DomainStartEndE-ValueType
Pfam:7tm_4 23 299 1.2e-71 PFAM
Pfam:7TM_GPCR_Srsx 27 297 3.9e-10 PFAM
Pfam:7tm_1 33 283 3.4e-16 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000071242
Predicted Effect probably benign
Transcript: ENSMUST00000098157
AA Change: V298I

PolyPhen 2 Score 0.110 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000095760
Gene: ENSMUSG00000095248
AA Change: V298I

DomainStartEndE-ValueType
Pfam:7tm_4 35 313 2e-72 PFAM
Pfam:7TM_GPCR_Srsx 39 311 6.1e-10 PFAM
Pfam:7tm_1 45 296 7.6e-15 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000214399
Predicted Effect noncoding transcript
Transcript: ENSMUST00000215180
Predicted Effect probably benign
Transcript: ENSMUST00000215517
AA Change: V298I

PolyPhen 2 Score 0.110 (Sensitivity: 0.93; Specificity: 0.86)
Predicted Effect probably benign
Transcript: ENSMUST00000215564
AA Change: V298I

PolyPhen 2 Score 0.110 (Sensitivity: 0.93; Specificity: 0.86)
Predicted Effect probably benign
Transcript: ENSMUST00000216247
AA Change: V298I

PolyPhen 2 Score 0.110 (Sensitivity: 0.93; Specificity: 0.86)
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.2%
  • 10x: 96.0%
  • 20x: 91.5%
Validation Efficiency 100% (57/57)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aadat T G 8: 60,531,763 probably benign Het
Abcb1b A T 5: 8,864,113 probably null Het
Adcy10 A G 1: 165,564,023 N1283S probably benign Het
Adgrb1 T A 15: 74,541,559 W531R probably damaging Het
Ago4 A G 4: 126,516,860 V246A probably benign Het
Arid5b A T 10: 68,186,033 D242E probably damaging Het
Arpp21 A G 9: 112,126,505 V522A probably benign Het
Atg4b T A 1: 93,784,910 probably benign Het
Capn12 T C 7: 28,887,683 F359S possibly damaging Het
Ccdc88c A T 12: 100,954,282 I360N probably damaging Het
Clic3 A G 2: 25,458,138 Y99C probably damaging Het
Cux1 T C 5: 136,307,859 E925G probably damaging Het
Dnah10 G A 5: 124,775,250 probably null Het
Dnah17 A G 11: 118,110,537 V860A possibly damaging Het
Etv5 C T 16: 22,436,075 probably benign Het
Fam83a T A 15: 58,009,811 N345K probably benign Het
G3bp1 T C 11: 55,498,626 F383L probably damaging Het
Gm13089 G T 4: 143,698,020 C284* probably null Het
Gpm6a A G 8: 55,055,374 probably null Het
Grid1 T A 14: 35,309,385 Y312N possibly damaging Het
Gstm4 T C 3: 108,043,525 N51S probably benign Het
Hid1 A T 11: 115,348,809 I765N probably damaging Het
Hmmr A G 11: 40,709,989 V518A unknown Het
Itgb6 A G 2: 60,669,197 V84A probably benign Het
Kbtbd4 A G 2: 90,907,604 K233E probably benign Het
Kif15 A T 9: 123,009,433 probably benign Het
Klre1 T C 6: 129,583,193 S143P probably damaging Het
Krt81 T C 15: 101,461,389 D216G probably benign Het
Mctp2 G T 7: 72,080,822 H868Q probably benign Het
Morc2b G C 17: 33,135,932 Y955* probably null Het
Myog A C 1: 134,290,473 N140H possibly damaging Het
Myrf G C 19: 10,218,162 T428S probably benign Het
Naip2 A C 13: 100,161,782 I582S probably benign Het
Neil3 A T 8: 53,638,775 probably null Het
Nrg1 T C 8: 31,831,245 probably null Het
Olfr689 A T 7: 105,314,372 M123L probably benign Het
Olfr99 A T 17: 37,280,291 L43* probably null Het
Pramef25 A T 4: 143,950,720 D96E possibly damaging Het
Ptger2 T A 14: 44,988,982 N6K possibly damaging Het
Ryr1 T A 7: 29,078,780 E2097V probably damaging Het
Sel1l A T 12: 91,820,094 F397Y probably damaging Het
Skint5 A G 4: 113,827,867 V551A unknown Het
Slc39a12 A G 2: 14,400,331 T245A probably benign Het
Syne1 C T 10: 5,358,438 V706I probably benign Het
Tbc1d8 G T 1: 39,372,774 Q994K possibly damaging Het
Tnrc6b C T 15: 80,876,653 T187I probably benign Het
Ttll6 G A 11: 96,154,756 A600T probably benign Het
Ust T C 10: 8,248,080 probably benign Het
Vmn2r71 GT GTT 7: 85,619,218 probably null Het
Vstm2a C T 11: 16,263,041 A142V probably damaging Het
Wfs1 T A 5: 36,973,722 probably benign Het
Wrap73 G A 4: 154,151,649 G145D probably damaging Het
Wrap73 G A 4: 154,156,154 V368M possibly damaging Het
Xrra1 T A 7: 99,875,145 probably null Het
Zfhx2 C T 14: 55,064,090 V2146I probably benign Het
Zfp335 A T 2: 164,907,922 L185* probably null Het
Other mutations in Olfr681
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00952:Olfr681 APN 7 105122407 splice site probably null
IGL01664:Olfr681 APN 7 105122216 missense probably damaging 1.00
IGL02691:Olfr681 APN 7 105122131 missense probably damaging 1.00
IGL02839:Olfr681 APN 7 105122356 missense probably damaging 1.00
R1139:Olfr681 UTSW 7 105121973 missense probably damaging 1.00
R1857:Olfr681 UTSW 7 105121544 missense probably benign 0.00
R4153:Olfr681 UTSW 7 105122309 missense probably damaging 0.99
R4391:Olfr681 UTSW 7 105121586 missense possibly damaging 0.60
R4537:Olfr681 UTSW 7 105127020 missense probably damaging 1.00
R4671:Olfr681 UTSW 7 105122306 missense probably damaging 1.00
R4789:Olfr681 UTSW 7 105122313 missense probably null 0.07
R5215:Olfr681 UTSW 7 105126564 missense probably damaging 1.00
R6080:Olfr681 UTSW 7 105121909 missense probably benign 0.19
R6194:Olfr681 UTSW 7 105122170 missense probably benign 0.07
R7054:Olfr681 UTSW 7 105121963 nonsense probably null
R7186:Olfr681 UTSW 7 105122266 missense probably benign 0.12
R7528:Olfr681 UTSW 7 105121864 missense probably damaging 1.00
R8035:Olfr681 UTSW 7 105121550 missense probably damaging 1.00
R8364:Olfr681 UTSW 7 105121703 missense probably damaging 1.00
R8433:Olfr681 UTSW 7 105121724 missense probably damaging 1.00
R8468:Olfr681 UTSW 7 105121478 missense probably benign
R9001:Olfr681 UTSW 7 105122240 missense probably damaging 1.00
R9129:Olfr681 UTSW 7 105122016 missense probably benign 0.00
R9453:Olfr681 UTSW 7 105121610 missense
R9705:Olfr681 UTSW 7 105121634 missense probably damaging 1.00
Z1176:Olfr681 UTSW 7 105122120 missense probably damaging 1.00
Z1176:Olfr681 UTSW 7 105122122 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CTGTAGCCAAAGCTCTGAGCACA -3'
(R):5'- TGAGGCCACTAATGTCTACTGTCACAA -3'

Sequencing Primer
(F):5'- ACATGTGGCTCTCACTTCATC -3'
(R):5'- ccacctgtctgtgcctc -3'
Posted On 2013-06-12