Mutagenetix > Incidental Mutation

Incidental Mutation 'R0535:Zfp58'

49435

Institutional Source

Beutler Lab

Gene Symbol

Zfp58

Ensembl Gene

ENSMUSG00000071291

Gene Name

zinc finger protein 58

Synonyms

Mfg1, Zfp817, A530094I17Rik, Mfg-1

MMRRC Submission

038727-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.126) question?

Stock #

R0535 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67638286-67648641 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 67640201 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 97 (Q97*)

Ref Sequence

ENSEMBL: ENSMUSP00000132285 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076123] [ENSMUST00000091523] [ENSMUST00000163534] [ENSMUST00000167914] [ENSMUST00000171518]

AlphaFold

P16372

Predicted Effect

probably null
Transcript: ENSMUST00000076123
AA Change: Q97*

SMART Domains

Protein: ENSMUSP00000075487
Gene: ENSMUSG00000071291
AA Change: Q97*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART
ZnF_C2H2	162	184	5.67e-5	SMART
ZnF_C2H2	190	212	3.21e-4	SMART
ZnF_C2H2	218	240	2.57e-3	SMART
ZnF_C2H2	246	268	2.91e-2	SMART
ZnF_C2H2	274	296	9.73e-4	SMART
ZnF_C2H2	302	324	6.23e-2	SMART
ZnF_C2H2	330	352	1.03e-2	SMART
ZnF_C2H2	358	380	1.45e-2	SMART
ZnF_C2H2	386	408	2.99e-4	SMART
ZnF_C2H2	410	432	4.87e-4	SMART
ZnF_C2H2	438	460	2.75e-3	SMART
ZnF_C2H2	466	488	1.58e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000091523
AA Change: Q97*

SMART Domains

Protein: ENSMUSP00000089108
Gene: ENSMUSG00000071291
AA Change: Q97*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART
ZnF_C2H2	162	184	5.67e-5	SMART
ZnF_C2H2	190	212	3.21e-4	SMART
ZnF_C2H2	218	240	2.57e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000163534
AA Change: Q97*

SMART Domains

Protein: ENSMUSP00000129177
Gene: ENSMUSG00000071291
AA Change: Q97*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000167914

SMART Domains

Protein: ENSMUSP00000137830
Gene: ENSMUSG00000097565

Domain	Start	End	E-Value	Type
KRAB	5	65	4.41e-34	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000171518
AA Change: Q97*

SMART Domains

Protein: ENSMUSP00000132285
Gene: ENSMUSG00000071291
AA Change: Q97*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART
ZnF_C2H2	162	184	5.67e-5	SMART
ZnF_C2H2	190	212	3.21e-4	SMART
ZnF_C2H2	218	240	2.57e-3	SMART
ZnF_C2H2	246	268	2.91e-2	SMART
ZnF_C2H2	274	296	9.73e-4	SMART
ZnF_C2H2	302	324	6.23e-2	SMART
ZnF_C2H2	330	352	1.03e-2	SMART
ZnF_C2H2	358	380	1.45e-2	SMART
ZnF_C2H2	386	408	2.99e-4	SMART
ZnF_C2H2	410	432	4.87e-4	SMART
ZnF_C2H2	438	460	2.75e-3	SMART
ZnF_C2H2	466	488	1.58e-3	SMART

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.6%
3x: 98.9%
10x: 97.0%
20x: 94.2%

Validation Efficiency

96% (52/54)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	A	G	11: 119,901,019 (GRCm39)	S1069P	probably benign	Het
Acmsd	A	T	1: 127,693,680 (GRCm39)	I305L	probably benign	Het
Aco2	G	A	15: 81,797,418 (GRCm39)	E625K	possibly damaging	Het
Acox1	G	A	11: 116,065,264 (GRCm39)	T561I	possibly damaging	Het
Acox2	T	A	14: 8,256,753 (GRCm38)	T37S	probably damaging	Het
Apc	A	T	18: 34,394,125 (GRCm39)	K17M	probably damaging	Het
Cant1	T	C	11: 118,301,969 (GRCm39)	D116G	probably damaging	Het
Col11a1	A	G	3: 113,855,184 (GRCm39)	E148G	unknown	Het
Cyp51	T	C	5: 4,149,202 (GRCm39)	Q225R	probably benign	Het
E2f8	T	C	7: 48,521,558 (GRCm39)		probably benign	Het
Fam163b	T	C	2: 27,002,778 (GRCm39)	Y73C	probably benign	Het
Fbxw9	T	C	8: 85,791,229 (GRCm39)	C271R	probably damaging	Het
Gbp10	T	C	5: 105,368,877 (GRCm39)	N321D	possibly damaging	Het
Gle1	T	C	2: 29,847,817 (GRCm39)	F675L	probably damaging	Het
Gm6327	T	C	16: 12,578,241 (GRCm39)		noncoding transcript	Het
Gphn	T	A	12: 78,538,824 (GRCm39)	F157I	possibly damaging	Het
Gtpbp1	A	T	15: 79,591,933 (GRCm39)	T94S	probably damaging	Het
Hdac2	T	C	10: 36,869,895 (GRCm39)	F286L	probably benign	Het
Ighv3-6	A	T	12: 114,252,090 (GRCm39)		probably benign	Het
Itga2b	A	G	11: 102,348,359 (GRCm39)	V791A	possibly damaging	Het
Itgb4	A	T	11: 115,881,835 (GRCm39)	I796F	possibly damaging	Het
Kel	T	C	6: 41,667,772 (GRCm39)	K390R	probably null	Het
Krt42	C	T	11: 100,155,412 (GRCm39)	C368Y	probably damaging	Het
Lancl1	A	G	1: 67,049,065 (GRCm39)		probably benign	Het
Lipg	A	G	18: 75,087,291 (GRCm39)	Y177H	probably damaging	Het
Lnpep	T	C	17: 17,791,935 (GRCm39)	E402G	possibly damaging	Het
Ltbp2	A	G	12: 84,837,826 (GRCm39)	F1185L	probably damaging	Het
Ltbp2	A	T	12: 84,831,632 (GRCm39)	I1727N	probably damaging	Het
Mettl22	T	C	16: 8,302,210 (GRCm39)		probably benign	Het
Mtcl2	T	C	2: 156,875,209 (GRCm39)	E847G	possibly damaging	Het
Mug1	G	A	6: 121,828,413 (GRCm39)	G275E	probably benign	Het
Nell2	T	A	15: 95,329,488 (GRCm39)	T278S	probably benign	Het
Nomo1	T	A	7: 45,721,941 (GRCm39)	S961T	probably damaging	Het
Or5ak22	A	G	2: 85,230,439 (GRCm39)	L146P	possibly damaging	Het
Pcare	A	G	17: 72,059,434 (GRCm39)	V81A	probably benign	Het
Phf2	A	G	13: 48,967,423 (GRCm39)	Y675H	unknown	Het
Phldb2	A	G	16: 45,577,490 (GRCm39)	V1145A	probably damaging	Het
Phrf1	T	C	7: 140,839,978 (GRCm39)	S1058P	probably benign	Het
Prss3	T	C	6: 41,351,903 (GRCm39)	N120S	probably benign	Het
Reln	G	T	5: 22,256,274 (GRCm39)		probably benign	Het
Spag5	A	G	11: 78,195,554 (GRCm39)	Y287C	probably benign	Het
Syde2	G	A	3: 145,694,925 (GRCm39)		probably null	Het
Synj1	C	A	16: 90,744,975 (GRCm39)	V1190F	possibly damaging	Het
Taok1	A	T	11: 77,444,530 (GRCm39)	I515N	probably benign	Het
Tmem259	A	T	10: 79,814,429 (GRCm39)	V309E	probably damaging	Het
Tmem62	T	A	2: 120,833,077 (GRCm39)	V494E	possibly damaging	Het
Trak1	A	T	9: 121,272,778 (GRCm39)	E119V	probably null	Het
Vmn1r1	T	A	1: 181,985,516 (GRCm39)	I50L	probably benign	Het
Vps35l	T	C	7: 118,347,404 (GRCm39)	F118S	possibly damaging	Het
Xpc	C	T	6: 91,481,560 (GRCm39)	V254I	possibly damaging	Het
Zscan5b	A	G	7: 6,236,911 (GRCm39)	E220G	possibly damaging	Het

Other mutations in Zfp58

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Zfp58	APN	13	67,639,114 (GRCm39)	missense	probably benign	0.29
IGL02618:Zfp58	APN	13	67,639,475 (GRCm39)	missense	possibly damaging	0.92
IGL03188:Zfp58	APN	13	67,639,528 (GRCm39)	missense	probably benign	0.03
R1470:Zfp58	UTSW	13	67,640,144 (GRCm39)	missense	possibly damaging	0.71
R1470:Zfp58	UTSW	13	67,640,144 (GRCm39)	missense	possibly damaging	0.71
R1750:Zfp58	UTSW	13	67,639,598 (GRCm39)	nonsense	probably null
R1862:Zfp58	UTSW	13	67,639,307 (GRCm39)	missense	probably damaging	1.00
R2697:Zfp58	UTSW	13	67,639,124 (GRCm39)	missense	probably damaging	1.00
R3031:Zfp58	UTSW	13	67,640,231 (GRCm39)	missense	probably benign	0.06
R3033:Zfp58	UTSW	13	67,639,741 (GRCm39)	missense	probably damaging	1.00
R4200:Zfp58	UTSW	13	67,639,440 (GRCm39)	missense	probably benign	0.25
R5827:Zfp58	UTSW	13	67,639,412 (GRCm39)	missense	probably damaging	0.99
R6723:Zfp58	UTSW	13	67,642,192 (GRCm39)	missense	probably damaging	1.00
R7230:Zfp58	UTSW	13	67,640,082 (GRCm39)	nonsense	probably null
R7890:Zfp58	UTSW	13	67,640,114 (GRCm39)	missense	possibly damaging	0.95
R9189:Zfp58	UTSW	13	67,640,035 (GRCm39)	missense	possibly damaging	0.93
R9338:Zfp58	UTSW	13	67,639,394 (GRCm39)	missense	probably benign	0.43
R9477:Zfp58	UTSW	13	67,640,158 (GRCm39)	missense	probably damaging	1.00
R9587:Zfp58	UTSW	13	67,639,823 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCAGAATGACGCAATTTATGCCAAGGA -3'
(R):5'- ccactactacctggTGGGCCTTATTTC -3'

Sequencing Primer
(F):5'- gccttgccacattcttcac -3'
(R):5'- acctggTGGGCCTTATTTCTATAATC -3'

Posted On

2013-06-12