Incidental Mutation 'R5211:Fstl3'
ID499949
Institutional Source Beutler Lab
Gene Symbol Fstl3
Ensembl Gene ENSMUSG00000020325
Gene Namefollistatin-like 3
SynonymsFlrg, E030038F23Rik
MMRRC Submission 042785-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R5211 (G1)
Quality Score103
Status Not validated
Chromosome10
Chromosomal Location79777272-79782630 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 79780178 bp
ZygosityHeterozygous
Amino Acid Change Glutamine to Leucine at position 166 (Q166L)
Ref Sequence ENSEMBL: ENSMUSP00000020575 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020573] [ENSMUST00000020575] [ENSMUST00000169684]
Predicted Effect probably benign
Transcript: ENSMUST00000020573
SMART Domains Protein: ENSMUSP00000020573
Gene: ENSMUSG00000020323

DomainStartEndE-ValueType
signal peptide 1 37 N/A INTRINSIC
Tryp_SPc 39 264 1.53e-70 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000020575
AA Change: Q166L

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000020575
Gene: ENSMUSG00000020325
AA Change: Q166L

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
FOLN 96 118 4.13e-6 SMART
KAZAL 116 165 1.69e-11 SMART
FOLN 168 191 1.09e-5 SMART
KAZAL 197 241 1.02e-5 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000167987
SMART Domains Protein: ENSMUSP00000130448
Gene: ENSMUSG00000020323

DomainStartEndE-ValueType
Pfam:Trypsin 1 75 1.4e-10 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000168798
Predicted Effect probably benign
Transcript: ENSMUST00000169684
SMART Domains Protein: ENSMUSP00000132215
Gene: ENSMUSG00000020323

DomainStartEndE-ValueType
signal peptide 1 37 N/A INTRINSIC
Tryp_SPc 39 264 1.53e-70 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000170380
Predicted Effect probably benign
Transcript: ENSMUST00000171681
SMART Domains Protein: ENSMUSP00000131642
Gene: ENSMUSG00000020323

DomainStartEndE-ValueType
Tryp_SPc 1 87 3.16e-1 SMART
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. [provided by RefSeq, Jul 2008]
PHENOTYPE: Adult mice homozygous for a knock-out allele display increased pancreatic islet number and size, beta cell hyperplasia, hepatic steatosis, increased heart weight, mild hypertension, and alterations in glucose homeostasis and fat distribution. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9930012K11Rik A G 14: 70,156,784 S181P probably benign Het
Adgrf5 A T 17: 43,422,620 T112S probably benign Het
Ascc2 T C 11: 4,673,399 V545A possibly damaging Het
Bmp2 A T 2: 133,554,630 S69C probably damaging Het
Btaf1 T A 19: 36,996,562 I1378K probably benign Het
Ces2b C T 8: 104,835,063 T263I possibly damaging Het
Cntn5 A T 9: 9,704,889 V635D possibly damaging Het
Dock6 T C 9: 21,820,352 E1218G probably benign Het
Eml6 T C 11: 29,854,145 I319V probably benign Het
Esd T C 14: 74,741,192 S65P probably damaging Het
Fam71f2 A T 6: 29,286,099 K128* probably null Het
Gcn1l1 T A 5: 115,619,312 S2445T probably benign Het
Gfy C A 7: 45,177,858 L271F possibly damaging Het
Gjc2 A G 11: 59,177,458 V66A possibly damaging Het
Gm7271 C A 5: 76,516,403 T113K possibly damaging Het
Hist1h3d G T 13: 23,575,841 G14C possibly damaging Het
Itm2c T A 1: 85,906,528 V188E probably damaging Het
Jmjd1c C G 10: 67,232,016 S1766C probably damaging Het
Kcnn3 A G 3: 89,521,231 T255A probably benign Het
Kdm4d A C 9: 14,463,104 V486G probably benign Het
Krt18 T G 15: 102,031,453 I362S probably damaging Het
Lrrc8d G A 5: 105,813,740 R672Q probably damaging Het
Ly6f G A 15: 75,271,803 V120M probably damaging Het
Map3k4 A G 17: 12,232,434 V1524A possibly damaging Het
Mrgprh T C 17: 12,877,002 V43A probably benign Het
Mx2 A T 16: 97,547,433 M269L probably damaging Het
Myrfl C T 10: 116,798,630 V620I probably benign Het
Nlrp9b G A 7: 20,049,456 C908Y probably damaging Het
Nyap2 T A 1: 81,087,274 M1K probably null Het
Olfml3 A T 3: 103,737,199 H51Q probably benign Het
Olfr745 C T 14: 50,643,253 T324I possibly damaging Het
Pcdhb1 G A 18: 37,266,651 V552I probably benign Het
Pm20d1 T A 1: 131,806,909 I353N possibly damaging Het
Sbk2 A G 7: 4,962,967 F73L possibly damaging Het
Scn10a A C 9: 119,661,232 L548R possibly damaging Het
Slc45a2 C T 15: 11,027,785 T480I probably damaging Het
Slmap T C 14: 26,482,962 Y68C probably damaging Het
Syde2 T C 3: 146,001,338 V611A probably benign Het
Sympk A G 7: 19,035,889 M164V probably benign Het
Syt17 T C 7: 118,442,403 S43G probably benign Het
Ubr1 T C 2: 120,893,170 T1303A possibly damaging Het
Vmn2r100 A G 17: 19,525,995 Y535C possibly damaging Het
Other mutations in Fstl3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02680:Fstl3 APN 10 79778672 nonsense probably null
IGL03165:Fstl3 APN 10 79779965 missense probably benign 0.02
R0382:Fstl3 UTSW 10 79777307 missense probably benign
R2113:Fstl3 UTSW 10 79781178 missense probably damaging 0.96
R2964:Fstl3 UTSW 10 79781223 missense probably benign
R2965:Fstl3 UTSW 10 79781223 missense probably benign
R2966:Fstl3 UTSW 10 79781223 missense probably benign
R6225:Fstl3 UTSW 10 79780009 missense probably benign 0.08
R7389:Fstl3 UTSW 10 79780031 missense probably damaging 1.00
R7390:Fstl3 UTSW 10 79780031 missense probably damaging 1.00
R7484:Fstl3 UTSW 10 79780031 missense probably damaging 1.00
T0722:Fstl3 UTSW 10 79780163 missense probably damaging 1.00
X0003:Fstl3 UTSW 10 79780163 missense probably damaging 1.00
X0022:Fstl3 UTSW 10 79780067 missense probably benign 0.06
Z1176:Fstl3 UTSW 10 79781198 missense probably benign
Z1177:Fstl3 UTSW 10 79780108 nonsense probably null
Predicted Primers PCR Primer
(F):5'- TGTCCTCAGATTCCTGCGAC -3'
(R):5'- CTTGGGCAACAACCGGATAG -3'

Sequencing Primer
(F):5'- TCAGATTCCTGCGACGGAGTG -3'
(R):5'- TGCATAATCACACCTTCCCCTAGG -3'
Posted On2017-11-30