Incidental Mutation 'R0131:Myh8'
ID 500138
Institutional Source Beutler Lab
Gene Symbol Myh8
Ensembl Gene ENSMUSG00000055775
Gene Name myosin, heavy polypeptide 8, skeletal muscle, perinatal
Synonyms Myhsp, 4832426G23Rik, MyHC-pn, Myhs-p
MMRRC Submission 038416-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.818) question?
Stock # R0131 (G1)
Quality Score 196
Status Not validated
Chromosome 11
Chromosomal Location 67277124-67308634 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 67292188 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Asparagine to Aspartic acid at position 659 (N659D)
Ref Sequence ENSEMBL: ENSMUSP00000019625 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000019625]
AlphaFold P13542
Predicted Effect probably damaging
Transcript: ENSMUST00000019625
AA Change: N659D

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000019625
Gene: ENSMUSG00000055775
AA Change: N659D

DomainStartEndE-ValueType
Pfam:Myosin_N 37 76 2.1e-13 PFAM
MYSc 82 782 N/A SMART
IQ 783 805 5.44e-3 SMART
Pfam:Myosin_tail_1 846 1927 2.4e-164 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000108686
Predicted Effect noncoding transcript
Transcript: ENSMUST00000139052
Meta Mutation Damage Score 0.1382 question?
Coding Region Coverage
  • 1x: 98.8%
  • 3x: 97.2%
  • 10x: 90.2%
  • 20x: 71.5%
Validation Efficiency 87% (52/60)
MGI Phenotype FUNCTION: This gene encodes a myosin heavy chain. The encoded protein forms a hexamer with two heavy chains, two alkali light chains, and two regulatory light chain components. This complex functions in muscle contraction. This gene is located in a cluster of related genes on chromosome 11. [provided by RefSeq, Jun 2013]
Allele List at MGI
Other mutations in this stock
Total: 101 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9530053A07Rik A G 7: 28,137,615 (GRCm38) R320G probably damaging Het
Abca8b T A 11: 109,942,289 (GRCm38) Q1195L possibly damaging Het
Abcc12 A G 8: 86,531,568 (GRCm38) I773T probably benign Het
Adamtsl1 T A 4: 86,342,723 (GRCm38) I1057N possibly damaging Het
Adgrv1 A T 13: 81,502,995 (GRCm38) probably benign Het
Anxa5 G A 3: 36,450,672 (GRCm38) A247V probably damaging Het
Arntl2 C A 6: 146,828,103 (GRCm38) H471N probably benign Het
Ascc3 T G 10: 50,735,329 (GRCm38) W1589G probably damaging Het
Atp2b2 G A 6: 113,793,782 (GRCm38) P389S probably damaging Het
Bicd1 A G 6: 149,512,947 (GRCm38) E386G probably damaging Het
Bpifa6 T A 2: 153,982,931 (GRCm38) S9T probably benign Het
Cacna1c T C 6: 118,625,512 (GRCm38) I1428V probably damaging Het
Chd8 A G 14: 52,205,326 (GRCm38) V589A probably benign Het
Chrnb2 T C 3: 89,764,406 (GRCm38) M1V probably null Het
Cldnd1 T C 16: 58,732,992 (GRCm38) L232P probably damaging Het
Col16a1 T A 4: 130,067,096 (GRCm38) V449E unknown Het
Col3a1 T A 1: 45,328,868 (GRCm38) probably benign Het
Cttnbp2nl T G 3: 105,005,857 (GRCm38) K237T probably damaging Het
Cyc1 G A 15: 76,344,959 (GRCm38) V142I probably benign Het
Dapk3 A G 10: 81,192,307 (GRCm38) T265A probably benign Het
Ddx21 A T 10: 62,584,752 (GRCm38) M711K possibly damaging Het
Dlg5 A T 14: 24,138,649 (GRCm38) L1735Q probably damaging Het
Dse A G 10: 34,153,664 (GRCm38) Y341H probably damaging Het
Elmod2 A G 8: 83,319,504 (GRCm38) I148T probably damaging Het
Fam187b T A 7: 30,989,120 (GRCm38) V22E probably damaging Het
Faxc A G 4: 21,936,659 (GRCm38) D98G probably damaging Het
Fcrls A G 3: 87,258,959 (GRCm38) S170P possibly damaging Het
Fsip2 G A 2: 82,991,121 (GRCm38) D5733N probably benign Het
Gbe1 T C 16: 70,360,852 (GRCm38) probably benign Het
Gm1141 T C X: 71,939,555 (GRCm38) C378R possibly damaging Het
Gm4788 T A 1: 139,754,271 (GRCm38) T196S probably damaging Het
Gm6327 T C 16: 12,761,045 (GRCm38) noncoding transcript Het
Gm9745 T A 13: 8,940,527 (GRCm38) probably benign Het
H2-T24 T A 17: 36,014,986 (GRCm38) I238F probably damaging Het
Hectd4 A G 5: 121,333,024 (GRCm38) E2658G probably benign Het
Herc1 A C 9: 66,480,910 (GRCm38) I3826L probably benign Het
Hinfp A G 9: 44,299,763 (GRCm38) C67R probably damaging Het
Hp1bp3 C T 4: 138,237,209 (GRCm38) S348F probably damaging Het
Hspg2 T C 4: 137,551,887 (GRCm38) Y3094H probably damaging Het
Htr1f A G 16: 64,926,728 (GRCm38) V67A probably damaging Het
Iqcc T G 4: 129,616,599 (GRCm38) E374D probably damaging Het
Kcnj9 T C 1: 172,326,198 (GRCm38) T120A probably damaging Het
Kitl C T 10: 100,087,364 (GRCm38) P208S probably benign Het
Kmt2b A T 7: 30,583,921 (GRCm38) C296S probably damaging Het
Lgals4 A G 7: 28,834,232 (GRCm38) probably null Het
Lpcat4 A G 2: 112,246,748 (GRCm38) Y479C probably damaging Het
Lrrc74b T C 16: 17,553,152 (GRCm38) N227S probably damaging Het
Mdc1 T A 17: 35,852,581 (GRCm38) V1007D probably damaging Het
Mocos T G 18: 24,679,762 (GRCm38) I571S probably benign Het
Mylk T C 16: 34,875,504 (GRCm38) V203A probably benign Het
Myom2 A G 8: 15,083,329 (GRCm38) N407S probably damaging Het
Naip2 A G 13: 100,183,788 (GRCm38) V240A probably benign Het
Nap1l1 T C 10: 111,485,509 (GRCm38) S37P probably benign Het
Nin T G 12: 70,051,141 (GRCm38) K515T probably damaging Het
Npl T A 1: 153,509,118 (GRCm38) K258* probably null Het
Ntn4 T A 10: 93,644,707 (GRCm38) S98T possibly damaging Het
Olfr1037 T C 2: 86,085,500 (GRCm38) I92M probably damaging Het
Olfr177 C A 16: 58,872,906 (GRCm38) M81I probably benign Het
Olfr372 C T 8: 72,058,400 (GRCm38) T240M probably damaging Het
Olfr417 T C 1: 174,369,586 (GRCm38) V223A probably damaging Het
Olfr720 T C 14: 14,175,620 (GRCm38) D154G probably benign Het
Pate3 A G 9: 35,646,157 (GRCm38) C68R probably damaging Het
Pcdh15 A G 10: 74,170,608 (GRCm38) D106G probably null Het
Ppox C A 1: 171,279,275 (GRCm38) A192S possibly damaging Het
Prkdc T C 16: 15,713,653 (GRCm38) L1380S probably benign Het
Proc C T 18: 32,135,898 (GRCm38) M11I probably benign Het
Psd4 C A 2: 24,405,351 (GRCm38) A839E probably damaging Het
Psg21 A G 7: 18,654,868 (GRCm38) Y100H probably benign Het
Pten T A 19: 32,776,069 (GRCm38) V45E probably benign Het
Ptprn2 T G 12: 116,722,091 (GRCm38) F57V probably damaging Het
Ptprt C T 2: 162,278,110 (GRCm38) V146I probably benign Het
R3hdm2 T A 10: 127,498,453 (GRCm38) M915K probably damaging Het
Rab26 C T 17: 24,530,785 (GRCm38) probably null Het
Rab7b T C 1: 131,698,555 (GRCm38) L107P probably damaging Het
Rbm47 T A 5: 66,026,529 (GRCm38) T244S possibly damaging Het
Rhbdf2 C A 11: 116,605,344 (GRCm38) G122C probably damaging Het
Rnf213 A G 11: 119,430,361 (GRCm38) E1215G probably benign Het
Rprd2 T C 3: 95,774,361 (GRCm38) K407E probably damaging Het
Siah3 G A 14: 75,456,134 (GRCm38) V27I possibly damaging Het
Slc12a3 G A 8: 94,340,883 (GRCm38) probably benign Het
Slc14a2 T A 18: 78,192,123 (GRCm38) N280Y probably damaging Het
Slc17a3 C T 13: 23,855,858 (GRCm38) S293F probably damaging Het
Slc25a35 A G 11: 68,971,960 (GRCm38) Y247C probably damaging Het
Slc29a4 A G 5: 142,705,530 (GRCm38) D55G probably benign Het
Slc35d1 C T 4: 103,208,181 (GRCm38) V189I probably benign Het
Spg11 T C 2: 122,070,968 (GRCm38) E1497G probably damaging Het
Srrm1 G A 4: 135,340,573 (GRCm38) R322* probably null Het
Stac3 A T 10: 127,503,650 (GRCm38) R138S probably damaging Het
Tet3 C G 6: 83,368,788 (GRCm38) G1556R probably damaging Het
Tgfbr3 A G 5: 107,132,816 (GRCm38) S693P probably benign Het
Tmcc2 C T 1: 132,380,706 (GRCm38) G150D probably benign Het
Tmem216 T C 19: 10,554,606 (GRCm38) Y44C probably damaging Het
Tmem260 T A 14: 48,483,322 (GRCm38) C306* probably null Het
Tspyl1 A G 10: 34,283,089 (GRCm38) N270S probably damaging Het
Ubr4 A G 4: 139,464,051 (GRCm38) T4127A possibly damaging Het
Ugt2a2 T A 5: 87,474,861 (GRCm38) K293* probably null Het
Vmn2r102 A C 17: 19,678,763 (GRCm38) T456P probably benign Het
Vmn2r90 T A 17: 17,712,249 (GRCm38) S139R probably benign Het
Wrnip1 G A 13: 32,806,864 (GRCm38) V369I probably damaging Het
Zc3h12c T A 9: 52,126,623 (GRCm38) I305F possibly damaging Het
Zmym2 A G 14: 56,943,258 (GRCm38) N876D probably benign Het
Other mutations in Myh8
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00236:Myh8 APN 11 67,283,818 (GRCm38) missense probably damaging 0.97
IGL01020:Myh8 APN 11 67,283,403 (GRCm38) missense probably damaging 0.99
IGL01348:Myh8 APN 11 67,297,780 (GRCm38) missense probably damaging 1.00
IGL01382:Myh8 APN 11 67,301,973 (GRCm38) missense probably damaging 1.00
IGL01454:Myh8 APN 11 67,283,596 (GRCm38) missense probably damaging 1.00
IGL01457:Myh8 APN 11 67,292,679 (GRCm38) missense probably benign 0.00
IGL01472:Myh8 APN 11 67,288,379 (GRCm38) splice site probably benign
IGL01473:Myh8 APN 11 67,301,825 (GRCm38) critical splice donor site probably null
IGL01613:Myh8 APN 11 67,301,710 (GRCm38) missense probably benign 0.11
IGL01763:Myh8 APN 11 67,286,419 (GRCm38) missense probably benign 0.01
IGL01828:Myh8 APN 11 67,303,826 (GRCm38) missense possibly damaging 0.82
IGL01862:Myh8 APN 11 67,289,694 (GRCm38) nonsense probably null
IGL01905:Myh8 APN 11 67,284,651 (GRCm38) missense possibly damaging 0.90
IGL02280:Myh8 APN 11 67,283,372 (GRCm38) unclassified probably benign
IGL02386:Myh8 APN 11 67,294,440 (GRCm38) missense probably damaging 0.99
IGL02449:Myh8 APN 11 67,294,614 (GRCm38) critical splice donor site probably null
IGL02500:Myh8 APN 11 67,305,710 (GRCm38) missense probably benign 0.00
IGL02745:Myh8 APN 11 67,297,501 (GRCm38) missense possibly damaging 0.88
IGL02799:Myh8 APN 11 67,301,592 (GRCm38) splice site probably benign
IGL03063:Myh8 APN 11 67,288,205 (GRCm38) missense probably benign 0.00
IGL03223:Myh8 APN 11 67,283,818 (GRCm38) missense probably damaging 0.97
IGL03336:Myh8 APN 11 67,284,702 (GRCm38) missense probably damaging 1.00
IGL03338:Myh8 APN 11 67,298,346 (GRCm38) missense probably damaging 1.00
IGL03351:Myh8 APN 11 67,303,913 (GRCm38) missense possibly damaging 0.94
IGL03392:Myh8 APN 11 67,294,418 (GRCm38) missense probably damaging 1.00
BB003:Myh8 UTSW 11 67,278,906 (GRCm38) missense possibly damaging 0.94
BB009:Myh8 UTSW 11 67,294,604 (GRCm38) missense probably benign 0.00
BB013:Myh8 UTSW 11 67,278,906 (GRCm38) missense possibly damaging 0.94
BB019:Myh8 UTSW 11 67,294,604 (GRCm38) missense probably benign 0.00
PIT4354001:Myh8 UTSW 11 67,289,630 (GRCm38) missense probably benign 0.01
R0012:Myh8 UTSW 11 67,300,021 (GRCm38) missense probably benign 0.02
R0016:Myh8 UTSW 11 67,298,525 (GRCm38) missense probably damaging 1.00
R0016:Myh8 UTSW 11 67,298,525 (GRCm38) missense probably damaging 1.00
R0115:Myh8 UTSW 11 67,306,264 (GRCm38) splice site probably benign
R0131:Myh8 UTSW 11 67,292,188 (GRCm38) missense probably damaging 0.96
R0132:Myh8 UTSW 11 67,292,188 (GRCm38) missense probably damaging 0.96
R0238:Myh8 UTSW 11 67,301,692 (GRCm38) missense probably benign 0.00
R0238:Myh8 UTSW 11 67,301,692 (GRCm38) missense probably benign 0.00
R0239:Myh8 UTSW 11 67,301,692 (GRCm38) missense probably benign 0.00
R0239:Myh8 UTSW 11 67,301,692 (GRCm38) missense probably benign 0.00
R0393:Myh8 UTSW 11 67,306,017 (GRCm38) splice site probably benign
R0453:Myh8 UTSW 11 67,292,905 (GRCm38) missense probably benign 0.03
R0454:Myh8 UTSW 11 67,303,765 (GRCm38) nonsense probably null
R0466:Myh8 UTSW 11 67,298,579 (GRCm38) missense probably benign 0.01
R0487:Myh8 UTSW 11 67,302,011 (GRCm38) missense probably benign
R0511:Myh8 UTSW 11 67,284,507 (GRCm38) missense probably benign 0.01
R0557:Myh8 UTSW 11 67,301,798 (GRCm38) missense possibly damaging 0.88
R0589:Myh8 UTSW 11 67,298,627 (GRCm38) missense probably benign 0.00
R0658:Myh8 UTSW 11 67,284,532 (GRCm38) critical splice donor site probably null
R0782:Myh8 UTSW 11 67,289,754 (GRCm38) missense probably benign 0.16
R0829:Myh8 UTSW 11 67,283,500 (GRCm38) unclassified probably benign
R0845:Myh8 UTSW 11 67,286,264 (GRCm38) missense probably damaging 1.00
R0930:Myh8 UTSW 11 67,305,998 (GRCm38) missense possibly damaging 0.93
R0972:Myh8 UTSW 11 67,297,759 (GRCm38) missense probably damaging 1.00
R1132:Myh8 UTSW 11 67,297,131 (GRCm38) nonsense probably null
R1417:Myh8 UTSW 11 67,306,185 (GRCm38) missense probably damaging 1.00
R1478:Myh8 UTSW 11 67,292,725 (GRCm38) missense probably benign 0.23
R1497:Myh8 UTSW 11 67,289,812 (GRCm38) missense probably benign 0.00
R1605:Myh8 UTSW 11 67,301,671 (GRCm38) missense probably damaging 0.99
R1701:Myh8 UTSW 11 67,280,138 (GRCm38) missense probably damaging 1.00
R1950:Myh8 UTSW 11 67,279,004 (GRCm38) missense possibly damaging 0.75
R1989:Myh8 UTSW 11 67,292,724 (GRCm38) missense probably benign 0.00
R2010:Myh8 UTSW 11 67,297,164 (GRCm38) nonsense probably null
R2095:Myh8 UTSW 11 67,286,224 (GRCm38) missense probably benign 0.00
R2132:Myh8 UTSW 11 67,292,876 (GRCm38) missense probably damaging 1.00
R2152:Myh8 UTSW 11 67,294,469 (GRCm38) missense probably damaging 0.97
R2229:Myh8 UTSW 11 67,308,348 (GRCm38) missense probably damaging 0.98
R2302:Myh8 UTSW 11 67,286,239 (GRCm38) missense probably damaging 1.00
R2364:Myh8 UTSW 11 67,294,518 (GRCm38) missense probably benign 0.03
R2429:Myh8 UTSW 11 67,303,897 (GRCm38) missense probably benign 0.21
R2880:Myh8 UTSW 11 67,297,264 (GRCm38) missense probably damaging 0.97
R3692:Myh8 UTSW 11 67,301,918 (GRCm38) missense probably damaging 0.98
R3756:Myh8 UTSW 11 67,284,617 (GRCm38) unclassified probably benign
R3924:Myh8 UTSW 11 67,297,137 (GRCm38) missense probably damaging 0.99
R4172:Myh8 UTSW 11 67,292,421 (GRCm38) missense probably damaging 1.00
R4255:Myh8 UTSW 11 67,299,734 (GRCm38) missense probably benign
R4621:Myh8 UTSW 11 67,286,258 (GRCm38) missense probably damaging 1.00
R4623:Myh8 UTSW 11 67,286,258 (GRCm38) missense probably damaging 1.00
R4790:Myh8 UTSW 11 67,279,963 (GRCm38) missense probably damaging 0.99
R4914:Myh8 UTSW 11 67,292,684 (GRCm38) missense probably damaging 1.00
R5074:Myh8 UTSW 11 67,305,916 (GRCm38) missense possibly damaging 0.79
R5119:Myh8 UTSW 11 67,298,358 (GRCm38) missense probably damaging 1.00
R5159:Myh8 UTSW 11 67,288,353 (GRCm38) missense probably damaging 0.99
R5229:Myh8 UTSW 11 67,284,484 (GRCm38) missense probably damaging 0.96
R5320:Myh8 UTSW 11 67,286,263 (GRCm38) missense probably damaging 1.00
R5455:Myh8 UTSW 11 67,301,418 (GRCm38) missense possibly damaging 0.59
R5523:Myh8 UTSW 11 67,305,962 (GRCm38) missense possibly damaging 0.95
R5540:Myh8 UTSW 11 67,286,440 (GRCm38) missense probably benign 0.00
R5726:Myh8 UTSW 11 67,294,566 (GRCm38) missense possibly damaging 0.79
R5770:Myh8 UTSW 11 67,297,200 (GRCm38) missense probably damaging 1.00
R6135:Myh8 UTSW 11 67,297,500 (GRCm38) missense possibly damaging 0.51
R6253:Myh8 UTSW 11 67,301,967 (GRCm38) missense probably benign 0.06
R6318:Myh8 UTSW 11 67,299,341 (GRCm38) missense probably benign 0.00
R6432:Myh8 UTSW 11 67,298,579 (GRCm38) missense probably benign 0.01
R6452:Myh8 UTSW 11 67,305,739 (GRCm38) missense possibly damaging 0.88
R6452:Myh8 UTSW 11 67,292,449 (GRCm38) missense probably benign 0.27
R6512:Myh8 UTSW 11 67,289,662 (GRCm38) nonsense probably null
R6714:Myh8 UTSW 11 67,306,949 (GRCm38) missense probably damaging 1.00
R6842:Myh8 UTSW 11 67,284,655 (GRCm38) missense probably damaging 1.00
R7007:Myh8 UTSW 11 67,288,316 (GRCm38) missense probably benign 0.03
R7025:Myh8 UTSW 11 67,297,539 (GRCm38) missense probably benign 0.02
R7086:Myh8 UTSW 11 67,292,627 (GRCm38) splice site probably null
R7098:Myh8 UTSW 11 67,279,053 (GRCm38) missense probably benign 0.03
R7498:Myh8 UTSW 11 67,283,437 (GRCm38) missense possibly damaging 0.80
R7716:Myh8 UTSW 11 67,298,652 (GRCm38) missense possibly damaging 0.51
R7765:Myh8 UTSW 11 67,303,655 (GRCm38) missense probably benign 0.44
R7825:Myh8 UTSW 11 67,303,712 (GRCm38) missense possibly damaging 0.94
R7921:Myh8 UTSW 11 67,283,818 (GRCm38) missense probably damaging 0.97
R7926:Myh8 UTSW 11 67,278,906 (GRCm38) missense possibly damaging 0.94
R7932:Myh8 UTSW 11 67,294,604 (GRCm38) missense probably benign 0.00
R8003:Myh8 UTSW 11 67,299,760 (GRCm38) missense probably damaging 1.00
R8028:Myh8 UTSW 11 67,303,676 (GRCm38) missense possibly damaging 0.65
R8121:Myh8 UTSW 11 67,289,821 (GRCm38) missense probably benign 0.00
R8125:Myh8 UTSW 11 67,299,772 (GRCm38) missense possibly damaging 0.94
R8170:Myh8 UTSW 11 67,288,266 (GRCm38) missense probably benign 0.30
R8277:Myh8 UTSW 11 67,292,909 (GRCm38) missense probably benign 0.10
R8304:Myh8 UTSW 11 67,304,336 (GRCm38) missense possibly damaging 0.72
R8431:Myh8 UTSW 11 67,283,614 (GRCm38) missense possibly damaging 0.94
R8535:Myh8 UTSW 11 67,278,915 (GRCm38) missense probably damaging 1.00
R8795:Myh8 UTSW 11 67,283,377 (GRCm38) critical splice acceptor site probably benign
R8858:Myh8 UTSW 11 67,301,994 (GRCm38) missense possibly damaging 0.67
R8927:Myh8 UTSW 11 67,283,255 (GRCm38) missense probably benign 0.10
R8928:Myh8 UTSW 11 67,283,255 (GRCm38) missense probably benign 0.10
R9031:Myh8 UTSW 11 67,299,315 (GRCm38) missense possibly damaging 0.49
R9172:Myh8 UTSW 11 67,292,434 (GRCm38) missense possibly damaging 0.82
R9252:Myh8 UTSW 11 67,286,476 (GRCm38) missense probably damaging 1.00
R9365:Myh8 UTSW 11 67,283,806 (GRCm38) missense probably benign 0.42
R9468:Myh8 UTSW 11 67,306,904 (GRCm38) missense probably damaging 1.00
R9564:Myh8 UTSW 11 67,286,389 (GRCm38) missense probably benign 0.40
R9565:Myh8 UTSW 11 67,286,389 (GRCm38) missense probably benign 0.40
T0722:Myh8 UTSW 11 67,304,436 (GRCm38) missense probably benign 0.41
Z1088:Myh8 UTSW 11 67,298,592 (GRCm38) missense probably damaging 1.00
Z1176:Myh8 UTSW 11 67,303,674 (GRCm38) missense probably damaging 1.00
Z1177:Myh8 UTSW 11 67,308,355 (GRCm38) missense possibly damaging 0.64
Z1177:Myh8 UTSW 11 67,301,424 (GRCm38) missense probably damaging 0.99
Z1187:Myh8 UTSW 11 67,297,486 (GRCm38) missense probably benign
Z1188:Myh8 UTSW 11 67,297,486 (GRCm38) missense probably benign
Z1190:Myh8 UTSW 11 67,297,486 (GRCm38) missense probably benign
Z1191:Myh8 UTSW 11 67,297,486 (GRCm38) missense probably benign
Predicted Primers
Posted On 2017-12-01