Mutagenetix > Incidental Mutation

Incidental Mutation 'R4111:Zkscan2'

500490

Institutional Source

Beutler Lab

Gene Symbol

Zkscan2

Ensembl Gene

ENSMUSG00000030757

Gene Name

zinc finger with KRAB and SCAN domains 2

Synonyms

Zfp694, 9430065N20Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R4111 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

123074607-123099672 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

T to C at 123081907 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000041821 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042470]

AlphaFold

G3X952

Predicted Effect

probably benign
Transcript: ENSMUST00000042470

SMART Domains

Protein: ENSMUSP00000041821
Gene: ENSMUSG00000030757

Domain	Start	End	E-Value	Type
SCAN	41	148	1.62e-54	SMART
KRAB	222	282	1.71e-2	SMART
SANT	333	397	3.73e0	SMART
low complexity region	449	469	N/A	INTRINSIC
SANT	489	553	2.18e0	SMART
low complexity region	627	649	N/A	INTRINSIC
ZnF_C2H2	768	790	6.42e-4	SMART
ZnF_C2H2	796	818	7.9e-4	SMART
ZnF_C2H2	824	846	5.99e-4	SMART
ZnF_C2H2	852	874	3.21e-4	SMART
ZnF_C2H2	880	902	1.18e-2	SMART
ZnF_C2H2	908	930	8.81e-2	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000125356
AA Change: D13G

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000206148

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.5%
20x: 96.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	G	T	11: 109,684,980 (GRCm39)	C172*	probably null	Het
Acot10	A	T	15: 20,666,612 (GRCm39)	L43Q	probably damaging	Het
Alms1	G	A	6: 85,597,870 (GRCm39)	V1368I	probably benign	Het
Ambra1	T	C	2: 91,730,903 (GRCm39)	S894P	probably damaging	Het
Arhgef12	C	A	9: 42,883,570 (GRCm39)	G1320C	probably damaging	Het
Atg7	G	C	6: 114,690,255 (GRCm39)	G596R	probably damaging	Het
Birc6	T	C	17: 74,873,010 (GRCm39)	V423A	probably damaging	Het
Bltp3a	T	A	17: 28,105,064 (GRCm39)	L586*	probably null	Het
Cdh17	T	C	4: 11,814,628 (GRCm39)	S728P	probably damaging	Het
Ctu2	G	A	8: 123,203,256 (GRCm39)	R24Q	possibly damaging	Het
Dclk3	T	C	9: 111,298,148 (GRCm39)	I564T	probably damaging	Het
Ddx39b	T	A	17: 35,462,340 (GRCm39)	I42N	possibly damaging	Het
Defa39	T	C	8: 22,192,679 (GRCm39)	T106A	possibly damaging	Het
Dip2c	G	T	13: 9,687,137 (GRCm39)	G1254C	probably damaging	Het
Dzip1	T	A	14: 119,114,645 (GRCm39)	K837*	probably null	Het
Epha1	G	A	6: 42,335,772 (GRCm39)	T955M	possibly damaging	Het
Etfbkmt	T	C	6: 149,046,089 (GRCm39)		probably benign	Het
Etfrf1	T	C	6: 145,161,098 (GRCm39)	Y23H	probably damaging	Het
Fat3	G	A	9: 15,909,567 (GRCm39)	S2145F	probably damaging	Het
Gm5611	T	A	9: 16,941,989 (GRCm39)		noncoding transcript	Het
Gpr37l1	T	C	1: 135,095,008 (GRCm39)	T79A	possibly damaging	Het
Hrh3	C	A	2: 179,744,643 (GRCm39)	R99L	possibly damaging	Het
Hyls1	T	A	9: 35,472,714 (GRCm39)	Y234F	probably damaging	Het
Ifnar1	C	A	16: 91,293,046 (GRCm39)	P230T	probably damaging	Het
Impact	T	C	18: 13,109,090 (GRCm39)		probably null	Het
Kcna6	G	C	6: 126,716,737 (GRCm39)	R51G	probably damaging	Het
Lrpprc	G	A	17: 85,033,766 (GRCm39)	T1037M	probably benign	Het
Or10g9	C	A	9: 39,912,194 (GRCm39)	E110*	probably null	Het
Or4a2	C	A	2: 89,248,444 (GRCm39)	L104F	probably benign	Het
Or5an6	T	C	19: 12,371,665 (GRCm39)	F13L	probably damaging	Het
Or6n2	T	A	1: 173,896,999 (GRCm39)	I45N	probably damaging	Het
Pask	A	G	1: 93,238,540 (GRCm39)	V1315A	probably damaging	Het
Pramel16	T	A	4: 143,676,475 (GRCm39)	I210F	possibly damaging	Het
Prl7a2	A	T	13: 27,849,050 (GRCm39)	Y80N	possibly damaging	Het
Rhox3f	G	T	X: 36,763,672 (GRCm39)	E140*	probably null	Het
Rtn2	T	C	7: 19,020,769 (GRCm39)	S81P	probably damaging	Het
Sbf2	G	A	7: 110,027,449 (GRCm39)	P470S	probably damaging	Het
Sec31b	G	T	19: 44,512,968 (GRCm39)	T507N	possibly damaging	Het
Sox30	A	G	11: 45,908,041 (GRCm39)	Y736C	probably benign	Het
Srsf3-ps	A	T	11: 98,516,223 (GRCm39)	V50D	probably damaging	Het
Synj2	G	A	17: 6,058,240 (GRCm39)	G243S	probably benign	Het
Tbr1	C	T	2: 61,642,076 (GRCm39)	P184L	probably benign	Het
Tns1	T	C	1: 73,981,091 (GRCm39)	N1091S	probably damaging	Het
Trappc10	A	G	10: 78,032,264 (GRCm39)	F1008S	probably benign	Het
Ube4a	T	A	9: 44,860,247 (GRCm39)	I272F	probably damaging	Het
Vmn1r122	A	T	7: 20,867,438 (GRCm39)	S206T	probably damaging	Het
Vmn2r1	A	C	3: 63,997,176 (GRCm39)	K277N	probably benign	Het
Vps13a	T	C	19: 16,617,992 (GRCm39)	E2931G	probably damaging	Het
Wdr70	G	T	15: 8,006,472 (GRCm39)	Q360K	probably benign	Het
Wdr90	G	T	17: 26,068,342 (GRCm39)	Q1329K	possibly damaging	Het
Wrn	T	C	8: 33,842,183 (GRCm39)	N37S	probably benign	Het
Yap1	A	T	9: 7,938,432 (GRCm39)	*358K	probably null	Het
Zfp964	T	A	8: 70,116,754 (GRCm39)	S450R	probably benign	Het
Zmynd10	A	T	9: 107,426,251 (GRCm39)	K133*	probably null	Het

Other mutations in Zkscan2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00743:Zkscan2	APN	7	123,079,195 (GRCm39)	missense	probably damaging	1.00
IGL02098:Zkscan2	APN	7	123,099,064 (GRCm39)	missense	probably benign	0.02
IGL03093:Zkscan2	APN	7	123,094,073 (GRCm39)	missense	probably benign	0.01
R0016:Zkscan2	UTSW	7	123,099,219 (GRCm39)	start gained	probably benign
R0135:Zkscan2	UTSW	7	123,079,864 (GRCm39)	missense	possibly damaging	0.63
R0541:Zkscan2	UTSW	7	123,079,423 (GRCm39)	missense	possibly damaging	0.92
R0569:Zkscan2	UTSW	7	123,097,898 (GRCm39)	missense	probably benign	0.11
R1537:Zkscan2	UTSW	7	123,099,064 (GRCm39)	missense	possibly damaging	0.95
R1726:Zkscan2	UTSW	7	123,089,046 (GRCm39)	missense	probably damaging	1.00
R3792:Zkscan2	UTSW	7	123,084,225 (GRCm39)	missense	possibly damaging	0.95
R3802:Zkscan2	UTSW	7	123,094,365 (GRCm39)	intron	probably benign
R3803:Zkscan2	UTSW	7	123,094,365 (GRCm39)	intron	probably benign
R3804:Zkscan2	UTSW	7	123,094,365 (GRCm39)	intron	probably benign
R4012:Zkscan2	UTSW	7	123,097,883 (GRCm39)	missense	possibly damaging	0.76
R4605:Zkscan2	UTSW	7	123,097,947 (GRCm39)	missense	probably damaging	1.00
R4978:Zkscan2	UTSW	7	123,094,542 (GRCm39)	missense	possibly damaging	0.86
R5004:Zkscan2	UTSW	7	123,089,267 (GRCm39)	missense	probably damaging	1.00
R5163:Zkscan2	UTSW	7	123,099,090 (GRCm39)	missense	probably benign	0.01
R5753:Zkscan2	UTSW	7	123,079,923 (GRCm39)	missense	probably benign
R5830:Zkscan2	UTSW	7	123,079,323 (GRCm39)	missense	possibly damaging	0.47
R6153:Zkscan2	UTSW	7	123,088,993 (GRCm39)	missense	probably benign	0.06
R6912:Zkscan2	UTSW	7	123,099,196 (GRCm39)	start gained	probably benign
R7170:Zkscan2	UTSW	7	123,099,030 (GRCm39)	missense	possibly damaging	0.83
R7269:Zkscan2	UTSW	7	123,088,994 (GRCm39)	missense	probably benign
R7310:Zkscan2	UTSW	7	123,089,276 (GRCm39)	missense	possibly damaging	0.53
R7399:Zkscan2	UTSW	7	123,079,327 (GRCm39)	missense	probably damaging	0.98
R7624:Zkscan2	UTSW	7	123,097,994 (GRCm39)	missense	probably damaging	0.97
R7687:Zkscan2	UTSW	7	123,099,085 (GRCm39)	missense	probably benign	0.13
R8236:Zkscan2	UTSW	7	123,079,135 (GRCm39)	missense	probably benign	0.01
R8443:Zkscan2	UTSW	7	123,084,651 (GRCm39)	missense	probably damaging	1.00
R9365:Zkscan2	UTSW	7	123,079,591 (GRCm39)	missense	probably damaging	1.00
R9411:Zkscan2	UTSW	7	123,084,405 (GRCm39)	missense	probably damaging	1.00
R9531:Zkscan2	UTSW	7	123,088,837 (GRCm39)	missense	probably damaging	0.97
R9757:Zkscan2	UTSW	7	123,079,310 (GRCm39)	nonsense	probably null
Z1177:Zkscan2	UTSW	7	123,079,581 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGCCCCTTTGTCTTGCATATTT -3'
(R):5'- TGTTGAGGTCCTTGATCCAC -3'

Sequencing Primer
(F):5'- TTATATGCTCCAGTACAGGGGAATGC -3'
(R):5'- CTGTGTACAGACTGCTAGTTAGACC -3'

Posted On

2017-12-01