Mutagenetix > Incidental Mutation

Incidental Mutation 'R4611:Frem2'

500668

Institutional Source

Beutler Lab

Gene Symbol

Frem2

Ensembl Gene

ENSMUSG00000037016

Gene Name

Fras1 related extracellular matrix protein 2

Synonyms

my, ne, 6030440P17Rik, b2b1562Clo, 8430406N05Rik

MMRRC Submission

041822-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4611 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

53513938-53657355 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 53547807 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Serine at position 2116 (L2116S)

Ref Sequence

ENSEMBL: ENSMUSP00000088670 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091137]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000091137
AA Change: L2116S

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000088670
Gene: ENSMUSG00000037016
AA Change: L2116S

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
Pfam:Cadherin_3	249	388	4.3e-9	PFAM
Pfam:Cadherin_3	376	532	3e-34	PFAM
Pfam:Cadherin_3	516	665	7.5e-24	PFAM
Pfam:Cadherin_3	632	798	1.6e-21	PFAM
Pfam:Cadherin_3	763	910	1.2e-25	PFAM
Pfam:Cadherin_3	879	1027	5.1e-18	PFAM
Pfam:Cadherin_3	1015	1159	2.2e-20	PFAM
CA	1202	1293	4.8e-1	SMART
Pfam:Cadherin_3	1392	1503	9.8e-24	PFAM
Pfam:Cadherin_3	1504	1612	6.2e-28	PFAM
Pfam:Cadherin_3	1613	1743	5.3e-20	PFAM
Calx_beta	1748	1847	1.5e-5	SMART
Calx_beta	1860	1971	9.47e-12	SMART
Calx_beta	1985	2092	1.65e-11	SMART
Calx_beta	2105	2209	1.99e-5	SMART
Calx_beta	2227	2331	6.9e-14	SMART
transmembrane domain	3103	3125	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197519

Meta Mutation Damage Score

0.4052

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Kidney development is severely affected and syndactyly is common. Phenotypes of homozygous mutants are indistinguishable from those of Fras1 homozygous mutant. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	G	A	5: 109,737,393 (GRCm38)	R200C	probably benign	Het
Ak7	G	A	12: 105,713,575 (GRCm38)	V123M	probably benign	Het
Apc	T	C	18: 34,318,565 (GRCm38)	L2804P	probably damaging	Het
Apob	C	A	12: 8,011,331 (GRCm38)	A3271E	probably damaging	Het
Asl	G	T	5: 130,018,316 (GRCm38)	A147E	probably damaging	Het
Atp1a1	A	G	3: 101,586,943 (GRCm38)	V447A	probably benign	Het
AY358078	T	A	14: 51,826,075 (GRCm38)	C393S	possibly damaging	Het
Bcl11b	T	C	12: 107,916,530 (GRCm38)	K509E	probably damaging	Het
Btnl1	T	C	17: 34,379,725 (GRCm38)	I105T	probably damaging	Het
Btnl10	A	G	11: 58,920,357 (GRCm38)	T169A	probably damaging	Het
C8b	T	C	4: 104,790,644 (GRCm38)	I278T	probably damaging	Het
Ccdc66	T	C	14: 27,500,420 (GRCm38)	N122S	probably damaging	Het
Cd8b1	T	C	6: 71,332,475 (GRCm38)	V181A	probably benign	Het
Cdan1	A	T	2: 120,730,720 (GRCm38)	V189D	probably damaging	Het
Celf2	G	T	2: 6,586,020 (GRCm38)	N279K	possibly damaging	Het
Cfap52	T	C	11: 67,926,421 (GRCm38)	N549D	probably damaging	Het
Cmklr1	C	T	5: 113,614,869 (GRCm38)	V24M	probably benign	Het
Cntnap4	G	T	8: 112,773,739 (GRCm38)		probably null	Het
Col27a1	G	A	4: 63,293,506 (GRCm38)	G91R	probably damaging	Het
Col5a3	A	G	9: 20,814,896 (GRCm38)		probably benign	Het
Dcc	C	T	18: 71,548,998 (GRCm38)		probably null	Het
Deaf1	T	A	7: 141,310,971 (GRCm38)	T433S	possibly damaging	Het
Dnah8	T	A	17: 30,684,237 (GRCm38)	L950H	probably damaging	Het
Dnajc7	A	T	11: 100,590,977 (GRCm38)	Y228*	probably null	Het
Doxl2	A	T	6: 48,975,156 (GRCm38)	Q5L	probably benign	Het
Eno1b	T	C	18: 48,047,703 (GRCm38)	V316A	probably damaging	Het
Exoc4	T	C	6: 33,438,405 (GRCm38)	M404T	possibly damaging	Het
Fignl1	A	T	11: 11,801,268 (GRCm38)	C596S	probably benign	Het
Fn1	A	C	1: 71,624,178 (GRCm38)	Y1050*	probably null	Het
Gba2	T	A	4: 43,568,092 (GRCm38)	S732C	probably damaging	Het
Gcnt4	C	A	13: 96,946,482 (GRCm38)	S95R	probably benign	Het
Gpr37	A	G	6: 25,669,624 (GRCm38)	V407A	probably benign	Het
Gria2	A	T	3: 80,692,492 (GRCm38)	M695K	probably damaging	Het
Hoxa9	T	C	6: 52,225,710 (GRCm38)	K132R	probably damaging	Het
Ifi213	T	A	1: 173,589,914 (GRCm38)	T311S	possibly damaging	Het
Itgb2	T	A	10: 77,550,050 (GRCm38)	N282K	probably damaging	Het
Kcnh8	A	T	17: 52,602,836 (GRCm38)	Q11L	probably benign	Het
Klk14	T	C	7: 43,694,357 (GRCm38)	C163R	probably damaging	Het
Klk1b3	T	A	7: 44,201,265 (GRCm38)	W74R	possibly damaging	Het
Krtap13-1	G	T	16: 88,729,254 (GRCm38)	C122F	possibly damaging	Het
Luzp2	T	C	7: 55,063,356 (GRCm38)		probably null	Het
Mbtps1	A	T	8: 119,535,347 (GRCm38)	D354E	probably damaging	Het
Mcpt9	C	T	14: 56,028,592 (GRCm38)	V60M	probably damaging	Het
Mical3	C	A	6: 120,934,838 (GRCm38)	E1083*	probably null	Het
Mrc2	G	A	11: 105,348,431 (GRCm38)		probably null	Het
Mtor	T	A	4: 148,486,119 (GRCm38)	V1211E	probably benign	Het
Myb	T	A	10: 21,145,324 (GRCm38)	D402V	probably damaging	Het
Ncor2	G	A	5: 125,030,859 (GRCm38)	T1593I	probably damaging	Het
Ndst3	A	G	3: 123,671,549 (GRCm38)	I258T	probably benign	Het
Npy6r	C	A	18: 44,276,401 (GRCm38)	H296Q	probably damaging	Het
Olfml2b	T	A	1: 170,644,947 (GRCm38)	L9H	probably damaging	Het
Olfr1364	A	T	13: 21,573,574 (GRCm38)	V294D	probably damaging	Het
Olfr1442	T	A	19: 12,674,954 (GRCm38)	Y250N	probably damaging	Het
Olfr145	T	A	9: 37,898,326 (GRCm38)	S307R	probably benign	Het
Olfr1475	C	T	19: 13,480,012 (GRCm38)	C62Y	probably damaging	Het
Olfr43	A	T	11: 74,206,535 (GRCm38)	V227D	possibly damaging	Het
Olfr519	A	G	7: 108,894,117 (GRCm38)	C97R	probably damaging	Het
Olfr727	C	A	14: 50,127,073 (GRCm38)	N165K	probably benign	Het
Olfr777	G	A	10: 129,268,405 (GRCm38)	A306V	probably benign	Het
Ovgp1	T	C	3: 105,986,567 (GRCm38)		probably benign	Het
Parg	G	A	14: 32,274,864 (GRCm38)	R304Q	probably damaging	Het
Pdf	T	G	8: 107,048,535 (GRCm38)	S22R	probably benign	Het
Phlpp2	A	T	8: 109,876,883 (GRCm38)	R60S	possibly damaging	Het
Piwil2	C	T	14: 70,402,197 (GRCm38)	E401K	probably benign	Het
Pla2g4e	T	G	2: 120,186,382 (GRCm38)	H226P	possibly damaging	Het
Polr3a	T	C	14: 24,452,508 (GRCm38)		probably null	Het
Ppp3cb	T	C	14: 20,520,646 (GRCm38)	N339S	possibly damaging	Het
Ptch2	G	A	4: 117,110,378 (GRCm38)	D748N	probably benign	Het
Rai14	A	C	15: 10,592,138 (GRCm38)	Y224D	probably damaging	Het
Rft1	A	G	14: 30,689,790 (GRCm38)	I432V	probably damaging	Het
Rictor	C	T	15: 6,787,144 (GRCm38)	A1299V	possibly damaging	Het
Sall2	A	G	14: 52,313,753 (GRCm38)	C662R	probably damaging	Het
Scn4b	A	G	9: 45,150,439 (GRCm38)	N208D	probably damaging	Het
Sdhb	G	A	4: 140,972,915 (GRCm38)	G109R	probably damaging	Het
Sec16a	A	G	2: 26,441,805 (GRCm38)	V66A	probably benign	Het
Sema4g	A	G	19: 45,001,612 (GRCm38)	Y710C	probably damaging	Het
Serpinb12	T	A	1: 106,949,153 (GRCm38)	D66E	probably benign	Het
Shc4	T	A	2: 125,655,682 (GRCm38)	D369V	probably benign	Het
Siglech	T	C	7: 55,771,693 (GRCm38)	L285P	probably damaging	Het
Skint6	A	G	4: 113,074,076 (GRCm38)	M506T	probably benign	Het
Slco1a6	C	A	6: 142,101,652 (GRCm38)	C404F	probably benign	Het
Slfn14	T	A	11: 83,283,314 (GRCm38)	K284*	probably null	Het
Sox14	A	G	9: 99,875,662 (GRCm38)	I8T	probably damaging	Het
Srpk3	A	G	X: 73,774,941 (GRCm38)	H79R	possibly damaging	Het
Tdpoz3	A	T	3: 93,827,023 (GRCm38)	H335L	probably damaging	Het
Tdrd5	T	A	1: 156,284,374 (GRCm38)	T479S	probably benign	Het
Tnik	T	C	3: 28,542,100 (GRCm38)		probably null	Het
Tnpo2	T	C	8: 85,053,803 (GRCm38)	S720P	probably benign	Het
Tpst1	A	G	5: 130,101,706 (GRCm38)	K6E	probably damaging	Het
Trank1	T	A	9: 111,362,261 (GRCm38)	I446N	probably damaging	Het
Trmt44	A	T	5: 35,575,007 (GRCm38)	D13E	probably benign	Het
Ubr4	T	A	4: 139,399,579 (GRCm38)	V471E	possibly damaging	Het
Ubtd1	A	G	19: 42,033,591 (GRCm38)	T101A	probably benign	Het
Ugt3a1	A	T	15: 9,306,400 (GRCm38)	K212*	probably null	Het
Zbtb7c	C	T	18: 76,136,847 (GRCm38)	A2V	possibly damaging	Het
Zfp423	C	T	8: 87,688,081 (GRCm38)	G1182D	possibly damaging	Het
Zfp618	G	A	4: 63,132,979 (GRCm38)	V666M	probably damaging	Het
Zmat1	A	T	X: 134,972,945 (GRCm38)	S566T	probably damaging	Homo
Zscan20	T	C	4: 128,588,106 (GRCm38)	T588A	probably benign	Het
Zscan25	G	A	5: 145,291,116 (GRCm38)	R530H	probably damaging	Het

Other mutations in Frem2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00895:Frem2	APN	3	53,585,595 (GRCm38)	missense	probably damaging	1.00
IGL00911:Frem2	APN	3	53,572,462 (GRCm38)	missense	probably damaging	1.00
IGL01322:Frem2	APN	3	53,541,038 (GRCm38)	missense	probably benign	0.00
IGL01330:Frem2	APN	3	53,655,241 (GRCm38)	missense	possibly damaging	0.70
IGL01406:Frem2	APN	3	53,525,896 (GRCm38)	missense	probably damaging	1.00
IGL01556:Frem2	APN	3	53,535,281 (GRCm38)	missense	probably benign	0.23
IGL01580:Frem2	APN	3	53,655,175 (GRCm38)	missense	probably damaging	1.00
IGL01606:Frem2	APN	3	53,653,591 (GRCm38)	missense	possibly damaging	0.69
IGL01611:Frem2	APN	3	53,655,709 (GRCm38)	missense	probably benign	0.00
IGL01648:Frem2	APN	3	53,535,732 (GRCm38)	missense	possibly damaging	0.86
IGL01663:Frem2	APN	3	53,517,013 (GRCm38)	missense	probably damaging	1.00
IGL01665:Frem2	APN	3	53,549,662 (GRCm38)	missense	probably benign	0.07
IGL01670:Frem2	APN	3	53,656,937 (GRCm38)	missense	possibly damaging	0.95
IGL01960:Frem2	APN	3	53,522,304 (GRCm38)	missense	probably benign	0.33
IGL02175:Frem2	APN	3	53,655,599 (GRCm38)	missense	possibly damaging	0.69
IGL02201:Frem2	APN	3	53,519,640 (GRCm38)	missense	probably benign	0.35
IGL02202:Frem2	APN	3	53,654,799 (GRCm38)	missense	probably benign	0.00
IGL02427:Frem2	APN	3	53,535,763 (GRCm38)	missense	probably damaging	0.97
IGL02457:Frem2	APN	3	53,521,049 (GRCm38)	missense	probably damaging	0.99
IGL02638:Frem2	APN	3	53,551,346 (GRCm38)	missense	possibly damaging	0.94
IGL02801:Frem2	APN	3	53,652,175 (GRCm38)	missense	possibly damaging	0.85
IGL03023:Frem2	APN	3	53,655,628 (GRCm38)	missense	probably benign	0.40
IGL03169:Frem2	APN	3	53,522,292 (GRCm38)	missense	probably benign	0.01
IGL03238:Frem2	APN	3	53,656,261 (GRCm38)	missense	possibly damaging	0.93
IGL03251:Frem2	APN	3	53,572,308 (GRCm38)	missense	probably benign	0.01
IGL03273:Frem2	APN	3	53,537,509 (GRCm38)	nonsense	probably null
IGL03343:Frem2	APN	3	53,652,253 (GRCm38)	missense	probably damaging	1.00
Biosimilar	UTSW	3	53,654,323 (GRCm38)	missense	probably benign	0.01
Fruit_stripe	UTSW	3	53,537,489 (GRCm38)	missense	probably benign	0.21
PIT4366001:Frem2	UTSW	3	53,653,201 (GRCm38)	missense	probably damaging	0.98
R0019:Frem2	UTSW	3	53,523,678 (GRCm38)	missense	probably damaging	0.99
R0092:Frem2	UTSW	3	53,589,796 (GRCm38)	missense	probably benign	0.03
R0108:Frem2	UTSW	3	53,647,961 (GRCm38)	missense	probably benign	0.03
R0115:Frem2	UTSW	3	53,656,208 (GRCm38)	missense	probably damaging	0.99
R0118:Frem2	UTSW	3	53,535,243 (GRCm38)	nonsense	probably null
R0374:Frem2	UTSW	3	53,653,960 (GRCm38)	missense	probably damaging	1.00
R0437:Frem2	UTSW	3	53,653,015 (GRCm38)	missense	possibly damaging	0.96
R0531:Frem2	UTSW	3	53,519,954 (GRCm38)	missense	probably damaging	1.00
R0555:Frem2	UTSW	3	53,516,860 (GRCm38)	missense	probably damaging	0.97
R0564:Frem2	UTSW	3	53,656,109 (GRCm38)	missense	probably damaging	0.97
R0586:Frem2	UTSW	3	53,647,921 (GRCm38)	missense	probably damaging	0.99
R0726:Frem2	UTSW	3	53,519,626 (GRCm38)	missense	possibly damaging	0.89
R0925:Frem2	UTSW	3	53,653,973 (GRCm38)	missense	probably benign
R1233:Frem2	UTSW	3	53,547,778 (GRCm38)	missense	probably damaging	0.98
R1302:Frem2	UTSW	3	53,655,538 (GRCm38)	missense	probably benign	0.00
R1333:Frem2	UTSW	3	53,549,731 (GRCm38)	missense	probably benign	0.26
R1446:Frem2	UTSW	3	53,654,596 (GRCm38)	missense	probably benign	0.31
R1523:Frem2	UTSW	3	53,655,407 (GRCm38)	missense	possibly damaging	0.73
R1539:Frem2	UTSW	3	53,654,210 (GRCm38)	missense	probably benign	0.19
R1543:Frem2	UTSW	3	53,572,455 (GRCm38)	missense	possibly damaging	0.86
R1597:Frem2	UTSW	3	53,654,519 (GRCm38)	missense	probably benign	0.19
R1600:Frem2	UTSW	3	53,547,723 (GRCm38)	missense	probably damaging	1.00
R1678:Frem2	UTSW	3	53,519,938 (GRCm38)	missense	probably damaging	1.00
R1687:Frem2	UTSW	3	53,653,952 (GRCm38)	missense	probably benign
R1696:Frem2	UTSW	3	53,656,042 (GRCm38)	nonsense	probably null
R1758:Frem2	UTSW	3	53,653,357 (GRCm38)	missense	probably damaging	1.00
R1857:Frem2	UTSW	3	53,654,873 (GRCm38)	missense	probably benign	0.10
R1869:Frem2	UTSW	3	53,535,196 (GRCm38)	missense	probably benign	0.04
R1921:Frem2	UTSW	3	53,653,495 (GRCm38)	missense	possibly damaging	0.76
R1973:Frem2	UTSW	3	53,652,232 (GRCm38)	missense	probably benign	0.01
R2045:Frem2	UTSW	3	53,535,744 (GRCm38)	missense	probably damaging	1.00
R2113:Frem2	UTSW	3	53,652,922 (GRCm38)	missense	probably damaging	1.00
R2152:Frem2	UTSW	3	53,517,029 (GRCm38)	nonsense	probably null
R2164:Frem2	UTSW	3	53,537,330 (GRCm38)	missense	probably damaging	1.00
R2181:Frem2	UTSW	3	53,574,587 (GRCm38)	missense	possibly damaging	0.72
R2201:Frem2	UTSW	3	53,516,573 (GRCm38)	missense	probably benign
R2221:Frem2	UTSW	3	53,516,857 (GRCm38)	missense	probably benign	0.00
R2255:Frem2	UTSW	3	53,652,514 (GRCm38)	missense	probably damaging	0.96
R2280:Frem2	UTSW	3	53,572,423 (GRCm38)	missense	probably damaging	1.00
R3196:Frem2	UTSW	3	53,537,331 (GRCm38)	missense	probably damaging	1.00
R3716:Frem2	UTSW	3	53,572,360 (GRCm38)	missense	probably damaging	1.00
R3807:Frem2	UTSW	3	53,653,449 (GRCm38)	missense	probably benign	0.22
R3820:Frem2	UTSW	3	53,516,849 (GRCm38)	missense	probably damaging	1.00
R3821:Frem2	UTSW	3	53,652,415 (GRCm38)	missense	probably damaging	1.00
R3977:Frem2	UTSW	3	53,652,070 (GRCm38)	missense	probably benign	0.00
R3979:Frem2	UTSW	3	53,652,070 (GRCm38)	missense	probably benign	0.00
R4014:Frem2	UTSW	3	53,652,353 (GRCm38)	missense	probably benign	0.01
R4127:Frem2	UTSW	3	53,525,896 (GRCm38)	missense	probably damaging	1.00
R4195:Frem2	UTSW	3	53,539,268 (GRCm38)	missense	possibly damaging	0.90
R4196:Frem2	UTSW	3	53,539,268 (GRCm38)	missense	possibly damaging	0.90
R4374:Frem2	UTSW	3	53,545,502 (GRCm38)	missense	possibly damaging	0.61
R4427:Frem2	UTSW	3	53,539,162 (GRCm38)	critical splice donor site	probably null
R4428:Frem2	UTSW	3	53,654,338 (GRCm38)	missense	probably benign	0.40
R4559:Frem2	UTSW	3	53,654,321 (GRCm38)	missense	probably benign	0.01
R4600:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4602:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4610:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4661:Frem2	UTSW	3	53,655,443 (GRCm38)	missense	probably damaging	1.00
R4678:Frem2	UTSW	3	53,544,371 (GRCm38)	missense	probably benign	0.00
R4689:Frem2	UTSW	3	53,547,635 (GRCm38)	missense	probably benign	0.43
R4740:Frem2	UTSW	3	53,535,819 (GRCm38)	missense	probably benign	0.04
R4748:Frem2	UTSW	3	53,541,093 (GRCm38)	missense	probably damaging	1.00
R4790:Frem2	UTSW	3	53,516,741 (GRCm38)	missense	probably benign
R4809:Frem2	UTSW	3	53,653,895 (GRCm38)	missense	probably benign	0.01
R4930:Frem2	UTSW	3	53,656,315 (GRCm38)	missense	possibly damaging	0.93
R4971:Frem2	UTSW	3	53,539,183 (GRCm38)	missense	probably damaging	1.00
R5057:Frem2	UTSW	3	53,535,196 (GRCm38)	missense	probably benign	0.37
R5202:Frem2	UTSW	3	53,551,346 (GRCm38)	missense	probably benign	0.41
R5221:Frem2	UTSW	3	53,585,611 (GRCm38)	missense	probably damaging	1.00
R5231:Frem2	UTSW	3	53,522,295 (GRCm38)	missense	probably damaging	1.00
R5268:Frem2	UTSW	3	53,653,154 (GRCm38)	missense	probably damaging	0.96
R5480:Frem2	UTSW	3	53,656,507 (GRCm38)	nonsense	probably null
R5637:Frem2	UTSW	3	53,652,937 (GRCm38)	missense	probably damaging	0.97
R5664:Frem2	UTSW	3	53,652,490 (GRCm38)	missense	probably benign	0.33
R5698:Frem2	UTSW	3	53,652,505 (GRCm38)	missense	possibly damaging	0.89
R5744:Frem2	UTSW	3	53,655,959 (GRCm38)	missense	probably damaging	1.00
R5754:Frem2	UTSW	3	53,537,258 (GRCm38)	missense	probably damaging	1.00
R5808:Frem2	UTSW	3	53,652,563 (GRCm38)	missense	probably damaging	0.96
R5840:Frem2	UTSW	3	53,647,921 (GRCm38)	missense	probably damaging	0.99
R5874:Frem2	UTSW	3	53,537,489 (GRCm38)	missense	probably benign	0.21
R6050:Frem2	UTSW	3	53,653,012 (GRCm38)	missense	probably damaging	0.99
R6103:Frem2	UTSW	3	53,549,788 (GRCm38)	missense	probably benign	0.00
R6149:Frem2	UTSW	3	53,551,341 (GRCm38)	missense	probably damaging	0.98
R6182:Frem2	UTSW	3	53,647,969 (GRCm38)	missense	probably damaging	1.00
R6191:Frem2	UTSW	3	53,655,280 (GRCm38)	missense	probably benign	0.10
R6245:Frem2	UTSW	3	53,655,824 (GRCm38)	missense	probably benign	0.00
R6252:Frem2	UTSW	3	53,572,448 (GRCm38)	missense	probably damaging	1.00
R6393:Frem2	UTSW	3	53,585,640 (GRCm38)	missense	possibly damaging	0.91
R6416:Frem2	UTSW	3	53,572,378 (GRCm38)	missense	probably benign	0.01
R6595:Frem2	UTSW	3	53,549,784 (GRCm38)	missense	probably damaging	1.00
R6665:Frem2	UTSW	3	53,654,656 (GRCm38)	missense	probably damaging	1.00
R6708:Frem2	UTSW	3	53,585,501 (GRCm38)	missense	probably benign	0.00
R6751:Frem2	UTSW	3	53,653,665 (GRCm38)	missense	probably damaging	1.00
R6787:Frem2	UTSW	3	53,654,323 (GRCm38)	missense	probably benign	0.01
R6913:Frem2	UTSW	3	53,516,821 (GRCm38)	missense	probably damaging	1.00
R6916:Frem2	UTSW	3	53,547,688 (GRCm38)	missense	probably damaging	1.00
R7017:Frem2	UTSW	3	53,519,602 (GRCm38)	missense	probably benign	0.02
R7083:Frem2	UTSW	3	53,537,493 (GRCm38)	missense	probably damaging	0.99
R7108:Frem2	UTSW	3	53,653,513 (GRCm38)	missense	probably damaging	1.00
R7133:Frem2	UTSW	3	53,572,339 (GRCm38)	missense	possibly damaging	0.82
R7326:Frem2	UTSW	3	53,654,753 (GRCm38)	missense	probably damaging	1.00
R7341:Frem2	UTSW	3	53,654,495 (GRCm38)	missense	probably damaging	1.00
R7455:Frem2	UTSW	3	53,572,280 (GRCm38)	splice site	probably null
R7487:Frem2	UTSW	3	53,654,549 (GRCm38)	missense	probably benign	0.40
R7495:Frem2	UTSW	3	53,516,837 (GRCm38)	missense	probably benign	0.13
R7542:Frem2	UTSW	3	53,652,579 (GRCm38)	missense	probably damaging	1.00
R7636:Frem2	UTSW	3	53,653,247 (GRCm38)	missense	probably benign	0.00
R7703:Frem2	UTSW	3	53,522,168 (GRCm38)	missense	probably benign	0.01
R7750:Frem2	UTSW	3	53,523,682 (GRCm38)	missense	possibly damaging	0.83
R7849:Frem2	UTSW	3	53,572,374 (GRCm38)	missense	probably damaging	1.00
R7922:Frem2	UTSW	3	53,653,304 (GRCm38)	missense	probably damaging	0.98
R8008:Frem2	UTSW	3	53,652,910 (GRCm38)	missense	probably damaging	1.00
R8051:Frem2	UTSW	3	53,535,355 (GRCm38)	missense	probably benign	0.04
R8052:Frem2	UTSW	3	53,549,643 (GRCm38)	missense	probably benign	0.02
R8176:Frem2	UTSW	3	53,655,340 (GRCm38)	missense	possibly damaging	0.50
R8220:Frem2	UTSW	3	53,656,507 (GRCm38)	nonsense	probably null
R8397:Frem2	UTSW	3	53,653,141 (GRCm38)	missense	probably benign	0.00
R8410:Frem2	UTSW	3	53,539,177 (GRCm38)	missense	possibly damaging	0.60
R8697:Frem2	UTSW	3	53,525,828 (GRCm38)	missense	probably damaging	0.99
R9134:Frem2	UTSW	3	53,654,900 (GRCm38)	missense	probably damaging	1.00
R9183:Frem2	UTSW	3	53,520,065 (GRCm38)	missense	probably damaging	1.00
R9260:Frem2	UTSW	3	53,652,783 (GRCm38)	missense	probably damaging	1.00
R9267:Frem2	UTSW	3	53,657,083 (GRCm38)	start codon destroyed	probably null	0.00
R9299:Frem2	UTSW	3	53,656,559 (GRCm38)	missense	probably benign	0.37
R9378:Frem2	UTSW	3	53,651,989 (GRCm38)	missense	probably damaging	0.99
R9444:Frem2	UTSW	3	53,652,844 (GRCm38)	missense	probably benign	0.10
R9459:Frem2	UTSW	3	53,653,486 (GRCm38)	missense	probably benign
R9487:Frem2	UTSW	3	53,653,484 (GRCm38)	missense	possibly damaging	0.95
R9728:Frem2	UTSW	3	53,656,631 (GRCm38)	missense	probably benign	0.00
R9759:Frem2	UTSW	3	53,655,497 (GRCm38)	missense	possibly damaging	0.76
Z1177:Frem2	UTSW	3	53,655,607 (GRCm38)	missense	probably benign	0.31
Z1177:Frem2	UTSW	3	53,535,166 (GRCm38)	missense	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- ACATCGGTATTTGGGCGTTC -3'
(R):5'- ACGCTTATTCTACAAAAGAGGCC -3'

Sequencing Primer
(F):5'- GGCGTTCCTCGAAGTCCATC -3'
(R):5'- CATCTTCTCCACGGAAAAC -3'

Posted On

2017-12-01