Incidental Mutation 'R5495:Gramd3'
ID501128
Institutional Source Beutler Lab
Gene Symbol Gramd3
Ensembl Gene ENSMUSG00000001700
Gene NameGRAM domain containing 3
Synonyms
MMRRC Submission 043056-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R5495 (G1)
Quality Score225
Status Not validated
Chromosome18
Chromosomal Location56432132-56503792 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 56482622 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Threonine at position 163 (I163T)
Ref Sequence ENSEMBL: ENSMUSP00000068453 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000070166]
Predicted Effect probably damaging
Transcript: ENSMUST00000070166
AA Change: I163T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000068453
Gene: ENSMUSG00000001700
AA Change: I163T

DomainStartEndE-ValueType
low complexity region 69 82 N/A INTRINSIC
low complexity region 86 104 N/A INTRINSIC
GRAM 110 177 3.06e-23 SMART
transmembrane domain 342 364 N/A INTRINSIC
coiled coil region 404 430 N/A INTRINSIC
Coding Region Coverage
  • 1x: 98.4%
  • 3x: 97.3%
  • 10x: 95.3%
  • 20x: 91.4%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 46 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9130204L05Rik A T 3: 91,090,295 L38M possibly damaging Het
Akt2 T C 7: 27,636,169 probably null Het
Arhgap29 T G 3: 122,014,929 M844R probably damaging Het
Atp1a1 T G 3: 101,591,425 D184A probably benign Het
Bcl11a T A 11: 24,165,042 V795E possibly damaging Het
Casp12 T A 9: 5,353,797 I277N possibly damaging Het
Ccdc59 A G 10: 105,845,378 K164E probably damaging Het
D630003M21Rik C T 2: 158,220,511 G30S possibly damaging Het
Dgkd A G 1: 87,926,872 D632G probably damaging Het
Efr3a A G 15: 65,815,409 K56E possibly damaging Het
Egflam T A 15: 7,251,241 R434S probably damaging Het
Fancl C G 11: 26,397,801 A51G probably damaging Het
Fkbp7 T C 2: 76,663,294 Y185C probably damaging Het
Galc A G 12: 98,231,414 probably null Het
Galnt15 A G 14: 32,029,817 S109G probably damaging Het
Gm13757 T C 2: 88,446,057 T294A probably benign Het
Impa1 A G 3: 10,326,170 V80A probably benign Het
Itga10 T C 3: 96,647,371 M56T possibly damaging Het
Larp1b G T 3: 41,035,822 R135I probably damaging Het
Lgals12 C T 19: 7,604,130 A71T probably damaging Het
Lmbr1 A T 5: 29,346,853 L78* probably null Het
Lrat C A 3: 82,896,982 M229I probably benign Het
Mug2 A T 6: 122,079,650 M1185L probably damaging Het
Naprt T C 15: 75,893,847 probably null Het
Nfat5 A G 8: 107,368,447 I1107V probably benign Het
Nr4a2 T C 2: 57,112,375 Y22C probably damaging Het
Ogfod1 C A 8: 94,064,278 Q526K probably benign Het
Olfr139 G A 11: 74,044,785 T163I probably damaging Het
Olfr710 C A 7: 106,944,492 G170* probably null Het
Olfr957 T C 9: 39,511,145 T192A probably benign Het
Parp10 T G 15: 76,243,166 I24L probably benign Het
Pcdha11 A G 18: 37,011,026 T57A probably benign Het
Prdm8 A G 5: 98,185,306 E244G possibly damaging Het
Prl6a1 T C 13: 27,312,671 S3P possibly damaging Het
Rab11fip3 T A 17: 26,016,143 T18S probably damaging Het
Rfc4 T C 16: 23,122,254 probably benign Het
Rubcnl G T 14: 75,042,337 V387F possibly damaging Het
Serpinb12 T C 1: 106,956,421 L299P probably damaging Het
Sptbn5 G A 2: 120,046,484 probably benign Het
Taar4 A T 10: 23,961,283 I264F possibly damaging Het
Tdpoz4 A T 3: 93,797,499 T368S probably benign Het
Thsd7b T A 1: 129,595,833 H124Q probably damaging Het
Ugt1a6a A T 1: 88,139,024 Q184L probably benign Het
Vnn1 T A 10: 23,898,564 F168L probably damaging Het
Zan A G 5: 137,470,408 L267P probably damaging Het
Zswim8 A G 14: 20,722,286 S1621G probably damaging Het
Other mutations in Gramd3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01582:Gramd3 APN 18 56485416 missense probably damaging 0.99
IGL01903:Gramd3 APN 18 56474029 missense probably damaging 0.99
IGL02203:Gramd3 APN 18 56478954 critical splice donor site probably null
IGL03210:Gramd3 APN 18 56474098 missense probably benign
PIT4283001:Gramd3 UTSW 18 56489663 missense probably damaging 1.00
R0432:Gramd3 UTSW 18 56474069 missense probably benign 0.00
R1623:Gramd3 UTSW 18 56432351 missense probably benign 0.00
R4019:Gramd3 UTSW 18 56478954 critical splice donor site probably null
R4125:Gramd3 UTSW 18 56485224 missense probably damaging 1.00
R4750:Gramd3 UTSW 18 56432300 missense probably benign 0.44
R4927:Gramd3 UTSW 18 56485451 missense probably damaging 0.96
R5866:Gramd3 UTSW 18 56474036 missense possibly damaging 0.93
R6443:Gramd3 UTSW 18 56485385 missense probably benign 0.00
R6672:Gramd3 UTSW 18 56432336 missense possibly damaging 0.49
R7030:Gramd3 UTSW 18 56485249 missense probably damaging 1.00
R7099:Gramd3 UTSW 18 56491945 missense probably benign 0.01
R7162:Gramd3 UTSW 18 56485457 critical splice donor site probably null
R7854:Gramd3 UTSW 18 56478854 missense probably damaging 1.00
R7937:Gramd3 UTSW 18 56478854 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCCGGTAGCTGTGGAAATCC -3'
(R):5'- CAACCCATACTTAATGTTGTTTGCC -3'

Sequencing Primer
(F):5'- ATCCCAGTCAGGACAGTTCAGTG -3'
(R):5'- ATACTTAATGTTGTTTGCCCTCTGTG -3'
Posted On2017-12-01