Mutagenetix > Incidental Mutation

Incidental Mutation 'R5628:Osgin2'

501344

Institutional Source

Beutler Lab

Gene Symbol

Osgin2

Ensembl Gene

ENSMUSG00000041153

Gene Name

oxidative stress induced growth inhibitor family member 2

Synonyms

C230027H09Rik

MMRRC Submission

043167-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.269) question?

Stock #

R5628 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

15997121-16013888 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 15998998 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 208 (N208I)

Ref Sequence

ENSEMBL: ENSMUSP00000047473 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037198] [ENSMUST00000149891]

AlphaFold

Q3TEE9

Predicted Effect

probably benign
Transcript: ENSMUST00000037198
AA Change: N208I

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000047473
Gene: ENSMUSG00000041153
AA Change: N208I

Domain	Start	End	E-Value	Type
SCOP:d1lqta1	315	361	7e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134048

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148819

Predicted Effect

probably benign
Transcript: ENSMUST00000149891

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam25	A	G	8: 41,208,747 (GRCm39)	D671G	probably benign	Het
Ap3b1	A	G	13: 94,613,556 (GRCm39)	D685G	unknown	Het
Atp6v1h	G	A	1: 5,206,112 (GRCm39)	W358*	probably null	Het
Atr	T	A	9: 95,756,279 (GRCm39)	Y830*	probably null	Het
B3galnt2	A	T	13: 14,169,737 (GRCm39)		probably null	Het
Casz1	T	C	4: 149,030,553 (GRCm39)	Y1191H	probably damaging	Het
Cdc40	T	G	10: 40,727,049 (GRCm39)	E169D	probably benign	Het
Cep55	C	T	19: 38,058,396 (GRCm39)	Q330*	probably null	Het
Clcn1	T	C	6: 42,275,823 (GRCm39)	V315A	probably damaging	Het
Cmya5	A	G	13: 93,226,218 (GRCm39)	F2957L	probably damaging	Het
Dnah2	C	T	11: 69,349,746 (GRCm39)	R2399Q	probably benign	Het
Dync1li2	T	C	8: 105,147,224 (GRCm39)	N490S	possibly damaging	Het
Ephb3	T	C	16: 21,036,869 (GRCm39)	Y111H	probably damaging	Het
Fam186a	A	C	15: 99,839,628 (GRCm39)	H2205Q	possibly damaging	Het
Fat3	T	C	9: 15,877,392 (GRCm39)	Y3407C	probably damaging	Het
Fbxw8	A	G	5: 118,230,622 (GRCm39)	V393A	probably damaging	Het
Fnip1	A	T	11: 54,394,459 (GRCm39)	D965V	probably benign	Het
Gramd2a	T	C	9: 59,615,006 (GRCm39)	M3T	probably benign	Het
Kctd15	T	C	7: 34,339,720 (GRCm39)	D283G	probably damaging	Het
Kif9	C	T	9: 110,343,621 (GRCm39)	R547*	probably null	Het
Map4	A	G	9: 109,910,915 (GRCm39)	T245A	probably benign	Het
Mindy4	C	T	6: 55,237,579 (GRCm39)	L385F	probably damaging	Het
Myo7b	A	G	18: 32,107,240 (GRCm39)	C1252R	probably benign	Het
Myt1l	T	A	12: 29,861,620 (GRCm39)	I134N	unknown	Het
Or5m10b	T	A	2: 85,699,149 (GRCm39)	I71N	probably damaging	Het
Polr2b	G	A	5: 77,461,063 (GRCm39)	V29M	probably damaging	Het
Prdm15	T	A	16: 97,600,823 (GRCm39)	M812L	probably damaging	Het
Prr36	TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC	TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC	8: 4,266,273 (GRCm39)		probably benign	Het
Rev3l	T	A	10: 39,698,963 (GRCm39)	N1153K	probably damaging	Het
Rnf17	A	G	14: 56,724,409 (GRCm39)		probably null	Het
Rusc2	C	T	4: 43,425,348 (GRCm39)	T1151M	probably damaging	Het
Scrib	T	C	15: 75,921,389 (GRCm39)	T30A	possibly damaging	Het
Sephs1	T	C	2: 4,894,018 (GRCm39)	I73T	probably benign	Het
Sf3b1	C	T	1: 55,037,334 (GRCm39)	A861T	probably benign	Het
Shq1	A	G	6: 100,607,964 (GRCm39)	W316R	probably damaging	Het
Slc26a5	T	A	5: 22,021,974 (GRCm39)	D484V	probably benign	Het
Smg1	C	T	7: 117,753,924 (GRCm39)		probably benign	Het
Stard5	T	C	7: 83,282,355 (GRCm39)	I56T	probably benign	Het
Szt2	A	G	4: 118,230,414 (GRCm39)	V2653A	unknown	Het
Tmem217	A	T	17: 29,745,430 (GRCm39)	I100N	probably damaging	Het
Trpm2	C	T	10: 77,748,470 (GRCm39)	R1400Q	probably benign	Het
Vmn1r82	T	G	7: 12,039,205 (GRCm39)	N41K	probably damaging	Het
Zfp236	T	C	18: 82,675,247 (GRCm39)	D367G	probably damaging	Het
Zfyve1	A	T	12: 83,621,663 (GRCm39)	V244E	probably benign	Het

Other mutations in Osgin2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00391:Osgin2	APN	4	16,006,439 (GRCm39)	missense	probably damaging	1.00
IGL00767:Osgin2	APN	4	16,006,377 (GRCm39)	missense	probably damaging	1.00
IGL03164:Osgin2	APN	4	16,001,938 (GRCm39)	missense	probably benign	0.03
R1115:Osgin2	UTSW	4	15,998,085 (GRCm39)	missense	possibly damaging	0.87
R1515:Osgin2	UTSW	4	15,998,380 (GRCm39)	missense	probably benign
R1878:Osgin2	UTSW	4	16,005,493 (GRCm39)	missense	probably damaging	0.99
R1964:Osgin2	UTSW	4	15,998,358 (GRCm39)	missense	probably damaging	1.00
R2116:Osgin2	UTSW	4	16,008,648 (GRCm39)	missense	probably damaging	0.99
R4684:Osgin2	UTSW	4	16,001,946 (GRCm39)	missense	probably benign
R4890:Osgin2	UTSW	4	16,013,739 (GRCm39)	utr 5 prime	probably benign
R5982:Osgin2	UTSW	4	15,998,908 (GRCm39)	missense	probably benign
R6418:Osgin2	UTSW	4	16,006,410 (GRCm39)	missense	probably benign
R7239:Osgin2	UTSW	4	16,008,748 (GRCm39)	missense	probably benign	0.00
R8502:Osgin2	UTSW	4	16,005,493 (GRCm39)	missense	probably damaging	0.99
R9489:Osgin2	UTSW	4	15,998,427 (GRCm39)	missense	probably damaging	1.00
R9605:Osgin2	UTSW	4	15,998,427 (GRCm39)	missense	probably damaging	1.00
R9700:Osgin2	UTSW	4	16,006,455 (GRCm39)	missense	probably damaging	0.99
R9789:Osgin2	UTSW	4	15,998,957 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCTGCTATTCGCTGATAGCCT -3'
(R):5'- ATCCCCTAAAGAGTTTTGTTTCTTCT -3'

Sequencing Primer
(F):5'- GCTGATAGCCTCTGATTTCCCAG -3'
(R):5'- ACTGAACTTGGTTCCAGAGC -3'

Posted On

2017-12-01