Mutagenetix > Incidental Mutations

Incidental Mutation 'R6158:Tmem87a'

501885

Institutional Source

Beutler Lab

Gene Symbol

Tmem87a

Ensembl Gene

ENSMUSG00000033808

Gene Name

transmembrane protein 87A

Synonyms

A930025J12Rik

MMRRC Submission

044305-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.164) question?

Stock #

R6158 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

120355312-120404113 bp(-) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 120360103 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000087500 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090042] [ENSMUST00000090046] [ENSMUST00000110729]

Predicted Effect

probably null
Transcript: ENSMUST00000090042

SMART Domains

Protein: ENSMUSP00000087496
Gene: ENSMUSG00000033808

Domain	Start	End	E-Value	Type
Pfam:Lung_7-TM_R	184	471	1.1e-87	PFAM
low complexity region	480	486	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000090046

SMART Domains

Protein: ENSMUSP00000087500
Gene: ENSMUSG00000033808

Domain	Start	End	E-Value	Type
Pfam:Lung_7-TM_R	185	472	1.5e-85	PFAM
low complexity region	481	487	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000110729

SMART Domains

Protein: ENSMUSP00000106357
Gene: ENSMUSG00000033808

Domain	Start	End	E-Value	Type
Pfam:Lung_7-TM_R	184	472	2.4e-86	PFAM
low complexity region	481	487	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000124806

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136410

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.5%
20x: 92.6%

Validation Efficiency

98% (65/66)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	T	C	12: 71,170,636	V793A	possibly damaging	Het
2900092C05Rik	A	G	7: 12,512,672	T32A	probably benign	Het
Adprhl1	C	T	8: 13,224,977	V594M	possibly damaging	Het
Ano3	A	T	2: 110,665,875	Y845N	probably damaging	Het
Arhgap24	A	T	5: 102,892,912	I575L	probably benign	Het
Aurka	A	C	2: 172,363,596		probably null	Het
C1qtnf5	A	T	9: 44,108,970		probably benign	Het
Cacnb2	A	G	2: 14,985,601	D454G	possibly damaging	Het
Ccdc58	T	A	16: 36,089,929	V118D	probably damaging	Het
Chchd5	T	C	2: 129,130,517	L87P	probably damaging	Het
Col7a1	G	T	9: 108,964,603	R1377L	unknown	Het
Cpne8	A	C	15: 90,571,988	S191A	probably damaging	Het
Dhx30	A	T	9: 110,087,030	I671N	probably damaging	Het
Dnah12	A	T	14: 26,773,685	K1423N	possibly damaging	Het
Dnm3	T	C	1: 162,320,987	M272V	probably damaging	Het
Fat4	A	T	3: 38,983,262	S3688C	possibly damaging	Het
Frmpd1	A	T	4: 45,285,401	L1407F	probably damaging	Het
Fry	T	C	5: 150,454,572	S410P	probably damaging	Het
Gm11565	T	A	11: 99,914,918	C45*	probably null	Het
Gngt1	A	T	6: 3,994,311	R30*	probably null	Het
Htt	T	C	5: 34,907,086	I2943T	possibly damaging	Het
Kl	T	A	5: 150,988,853	M689K	possibly damaging	Het
Lmo7	T	A	14: 101,900,137	D247E	probably benign	Het
Mastl	G	T	2: 23,132,772	N646K	possibly damaging	Het
Mei4	G	T	9: 81,927,576	L237F	probably damaging	Het
Mettl27	C	T	5: 134,940,576	P170S	possibly damaging	Het
Mgam	T	A	6: 40,757,714	I896K	probably damaging	Het
Moxd1	T	A	10: 24,284,777	C443S	probably damaging	Het
Myo18b	A	T	5: 112,874,172	N451K	probably benign	Het
Myo7b	T	C	18: 31,988,549	I768V	probably benign	Het
Nos1	A	C	5: 117,867,574	I120L	probably benign	Het
Nsd1	T	C	13: 55,245,621	V345A	probably benign	Het
Olfr1095	A	G	2: 86,851,515	L61P	possibly damaging	Het
Olfr1205	T	C	2: 88,831,146	F10L	probably damaging	Het
Olfr150	C	T	9: 39,737,076	T87I	probably benign	Het
Olfr170	C	A	16: 19,605,925	V248F	probably damaging	Het
Olfr344	A	T	2: 36,569,116	T173S	probably benign	Het
Pdzph1	T	G	17: 58,973,627	Q553H	probably damaging	Het
Piwil1	T	A	5: 128,747,876	L546*	probably null	Het
Pla2g4f	T	C	2: 120,301,071	T724A	probably benign	Het
Ralgapa2	A	T	2: 146,424,676	M660K	possibly damaging	Het
Rgsl1	T	C	1: 153,804,021	D103G	possibly damaging	Het
Rnf186	A	G	4: 138,967,254	D35G	probably damaging	Het
Rock2	A	G	12: 16,954,918	D424G	probably benign	Het
Scg2	A	T	1: 79,435,400	D495E	probably damaging	Het
Slc39a2	G	A	14: 51,894,224		probably null	Het
Snrnp48	T	A	13: 38,210,236	Y100*	probably null	Het
Spaca1	A	G	4: 34,029,176	M99T	probably damaging	Het
Specc1	T	G	11: 62,118,124	F235L	probably damaging	Het
St13	A	T	15: 81,399,601		probably null	Het
Swap70	T	A	7: 110,270,023	M341K	probably damaging	Het
Synj2	A	G	17: 5,986,212	D67G	probably benign	Het
Tmem135	T	A	7: 89,156,444	I251F	probably benign	Het
Tom1l2	C	T	11: 60,232,927	D128N	probably damaging	Het
Tpx2	C	A	2: 152,873,104	H82N	probably benign	Het
Trip12	A	T	1: 84,761,012	C738S	possibly damaging	Het
Ttyh1	A	G	7: 4,125,562	T153A	probably benign	Het
Utrn	C	T	10: 12,690,822	G1199S	probably benign	Het
Vmn1r8	T	A	6: 57,036,289	N108K	probably benign	Het
Vmn2r63	T	A	7: 42,933,680	D37V	probably damaging	Het
Vwce	C	T	19: 10,644,221	R206C	possibly damaging	Het
Wrn	G	T	8: 33,319,172	F265L	probably damaging	Het
Zfp472	T	A	17: 32,978,389	C479*	probably null	Het
Zfp831	A	T	2: 174,643,858	T109S	possibly damaging	Het
Znfx1	G	T	2: 167,056,726	Q93K	probably benign	Het

Other mutations in Tmem87a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00491:Tmem87a	APN	2	120379780	splice site	probably benign
IGL00912:Tmem87a	APN	2	120403936	missense	possibly damaging	0.54
IGL01301:Tmem87a	APN	2	120380769	missense	probably benign	0.01
IGL01413:Tmem87a	APN	2	120385870	missense	probably benign	0.06
IGL01418:Tmem87a	APN	2	120385870	missense	probably benign	0.06
IGL02083:Tmem87a	APN	2	120397380	missense	probably damaging	1.00
IGL02150:Tmem87a	APN	2	120360076	missense	probably damaging	0.99
IGL02256:Tmem87a	APN	2	120377896	missense	probably damaging	1.00
IGL02314:Tmem87a	APN	2	120404021	missense	possibly damaging	0.57
IGL02501:Tmem87a	APN	2	120404053	missense	probably damaging	0.98
IGL02550:Tmem87a	APN	2	120374485	splice site	probably null
IGL03082:Tmem87a	APN	2	120397366	missense	possibly damaging	0.81
ANU18:Tmem87a	UTSW	2	120380769	missense	probably benign	0.01
R0254:Tmem87a	UTSW	2	120375507	missense	probably damaging	1.00
R0285:Tmem87a	UTSW	2	120394424	missense	probably benign	0.01
R0498:Tmem87a	UTSW	2	120394465	missense	probably benign	0.01
R0611:Tmem87a	UTSW	2	120375448	missense	possibly damaging	0.46
R0632:Tmem87a	UTSW	2	120359542	missense	probably damaging	1.00
R0787:Tmem87a	UTSW	2	120370484	missense	probably benign	0.22
R1599:Tmem87a	UTSW	2	120394387	missense	probably damaging	1.00
R1977:Tmem87a	UTSW	2	120374504	missense	probably benign	0.02
R2059:Tmem87a	UTSW	2	120369292	missense	probably damaging	1.00
R2396:Tmem87a	UTSW	2	120404059	start codon destroyed	probably null	0.02
R2496:Tmem87a	UTSW	2	120394378	missense	probably damaging	0.96
R4478:Tmem87a	UTSW	2	120369343	nonsense	probably null
R4621:Tmem87a	UTSW	2	120397424	missense	probably benign	0.00
R4739:Tmem87a	UTSW	2	120360037	critical splice donor site	probably null
R5138:Tmem87a	UTSW	2	120371545	missense	possibly damaging	0.88
R5314:Tmem87a	UTSW	2	120377926	missense	probably damaging	0.99
R5391:Tmem87a	UTSW	2	120362877	critical splice donor site	probably null
R5536:Tmem87a	UTSW	2	120397430	missense	probably damaging	0.96
R5618:Tmem87a	UTSW	2	120369306	missense	probably benign	0.44
R5642:Tmem87a	UTSW	2	120403946	missense	probably benign	0.00
R5884:Tmem87a	UTSW	2	120404124	unclassified	probably benign
R6104:Tmem87a	UTSW	2	120394424	missense	probably benign	0.01
R6195:Tmem87a	UTSW	2	120392175	splice site	probably null
R6233:Tmem87a	UTSW	2	120392175	splice site	probably null
R6261:Tmem87a	UTSW	2	120404021	missense	possibly damaging	0.57
R6403:Tmem87a	UTSW	2	120380771	missense	possibly damaging	0.94
R6405:Tmem87a	UTSW	2	120379750	missense	probably damaging	1.00
R6540:Tmem87a	UTSW	2	120403919	missense	probably benign	0.00
R6583:Tmem87a	UTSW	2	120375477	missense	possibly damaging	0.93
R6995:Tmem87a	UTSW	2	120362928	missense	possibly damaging	0.91
R7081:Tmem87a	UTSW	2	120380783	missense	possibly damaging	0.88
R7384:Tmem87a	UTSW	2	120371523	critical splice donor site	probably null
R7558:Tmem87a	UTSW	2	120374510	missense	probably benign	0.00
R7904:Tmem87a	UTSW	2	120379717	missense	probably damaging	1.00
R8124:Tmem87a	UTSW	2	120392195	missense	probably benign
R8165:Tmem87a	UTSW	2	120370478	missense	possibly damaging	0.95
R8259:Tmem87a	UTSW	2	120397447	missense	possibly damaging	0.65
R8315:Tmem87a	UTSW	2	120403960	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GAAATATCATGCAAGTGAGTAGTCC -3'
(R):5'- GGAGATGTTAGCCAAAGGTATTTG -3'

Sequencing Primer
(F):5'- CCTCAGTTTCCCACTGAAA -3'
(R):5'- GGATAACGAAAGGCTGCTA -3'

Posted On

2017-12-04