Incidental Mutation 'R6196:Hk1'
| ID |
502926 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Hk1
|
| Ensembl Gene |
ENSMUSG00000037012 |
| Gene Name |
hexokinase 1 |
| Synonyms |
mHk1-s, Hk1-s, Hk-1 |
| MMRRC Submission |
044336-MU
|
| Accession Numbers |
|
| Essential gene? |
Possibly non essential
(E-score: 0.430)
|
| Stock # |
R6196 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
10 |
| Chromosomal Location |
62104634-62215687 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 62135038 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Histidine to Leucine
at position 24
(H24L)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000120151
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000072357]
[ENSMUST00000099691]
[ENSMUST00000116238]
[ENSMUST00000130422]
[ENSMUST00000139228]
[ENSMUST00000143179]
[ENSMUST00000152761]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000072357
AA Change: H133L
PolyPhen 2
Score 0.266 (Sensitivity: 0.91; Specificity: 0.88)
|
| SMART Domains |
Protein: ENSMUSP00000072195
Gene: ENSMUSG00000037012
AA Change: H133L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Hexokinase_1
|
25 |
224 |
1.2e-70 |
PFAM |
|
Pfam:Hexokinase_2
|
229 |
486 |
8e-79 |
PFAM |
|
Pfam:Hexokinase_1
|
496 |
695 |
7e-76 |
PFAM |
|
Pfam:Hexokinase_2
|
700 |
934 |
4.2e-85 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000099691
AA Change: H129L
PolyPhen 2
Score 0.406 (Sensitivity: 0.89; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000097282
Gene: ENSMUSG00000037012
AA Change: H129L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Hexokinase_1
|
16 |
221 |
1.9e-86 |
PFAM |
|
Pfam:Hexokinase_2
|
223 |
462 |
1e-102 |
PFAM |
|
Pfam:Hexokinase_1
|
464 |
669 |
1.1e-90 |
PFAM |
|
Pfam:Hexokinase_2
|
671 |
910 |
2.2e-109 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000116238
AA Change: H133L
PolyPhen 2
Score 0.266 (Sensitivity: 0.91; Specificity: 0.88)
|
| SMART Domains |
Protein: ENSMUSP00000111946
Gene: ENSMUSG00000037012
AA Change: H133L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Hexokinase_1
|
21 |
225 |
1.3e-85 |
PFAM |
|
Pfam:Hexokinase_2
|
227 |
357 |
3.6e-56 |
PFAM |
|
Pfam:Hexokinase_2
|
362 |
489 |
9.3e-41 |
PFAM |
|
Pfam:Hexokinase_1
|
491 |
696 |
2e-90 |
PFAM |
|
Pfam:Hexokinase_2
|
698 |
937 |
3.8e-109 |
PFAM |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000130422
AA Change: H128L
PolyPhen 2
Score 0.869 (Sensitivity: 0.83; Specificity: 0.93)
|
| SMART Domains |
Protein: ENSMUSP00000118601
Gene: ENSMUSG00000037012
AA Change: H128L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Hexokinase_1
|
16 |
220 |
1.4e-85 |
PFAM |
|
Pfam:Hexokinase_2
|
222 |
461 |
1e-102 |
PFAM |
|
Pfam:Hexokinase_1
|
463 |
668 |
1.1e-90 |
PFAM |
|
Pfam:Hexokinase_2
|
670 |
909 |
2.2e-109 |
PFAM |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000139228
AA Change: H115L
PolyPhen 2
Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000118166
Gene: ENSMUSG00000037012
AA Change: H115L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Hexokinase_1
|
3 |
184 |
1.4e-74 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000142527
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000143179
AA Change: H24L
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000120151
Gene: ENSMUSG00000037012
AA Change: H24L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Hexokinase_1
|
5 |
80 |
5e-29 |
PFAM |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000152761
AA Change: H113L
PolyPhen 2
Score 0.957 (Sensitivity: 0.78; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000117752
Gene: ENSMUSG00000037012
AA Change: H113L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Hexokinase_1
|
2 |
205 |
5.6e-88 |
PFAM |
|
| Meta Mutation Damage Score |
0.4144 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.8%
|
| Validation Efficiency |
96% (55/57) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]
PHENOTYPE: Homozygous mutant mice exhibit hemolytic anemia with extensive tissue iron deposition and reticulocytosis and female infertility. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 55 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4933405L10Rik |
A |
T |
8: 106,436,554 (GRCm39) |
H241L |
possibly damaging |
Het |
| Acap1 |
T |
C |
11: 69,777,893 (GRCm39) |
D115G |
probably damaging |
Het |
| Acvr2b |
T |
C |
9: 119,262,469 (GRCm39) |
V510A |
possibly damaging |
Het |
| Acyp2 |
C |
T |
11: 30,456,354 (GRCm39) |
E98K |
possibly damaging |
Het |
| Agr2 |
A |
T |
12: 36,045,591 (GRCm39) |
K26* |
probably null |
Het |
| Aox4 |
T |
A |
1: 58,256,685 (GRCm39) |
I69N |
probably damaging |
Het |
| Asb4 |
G |
A |
6: 5,390,699 (GRCm39) |
G31R |
probably benign |
Het |
| Atp6v1c2 |
C |
A |
12: 17,351,187 (GRCm39) |
E105* |
probably null |
Het |
| Bend6 |
A |
G |
1: 33,917,509 (GRCm39) |
Y44H |
probably damaging |
Het |
| Bltp3b |
T |
G |
10: 89,641,195 (GRCm39) |
S789A |
probably benign |
Het |
| Btn2a2 |
C |
T |
13: 23,672,015 (GRCm39) |
V25M |
possibly damaging |
Het |
| Cab39l |
T |
A |
14: 59,737,039 (GRCm39) |
L53Q |
probably damaging |
Het |
| Cc2d1b |
C |
T |
4: 108,490,422 (GRCm39) |
R825W |
probably damaging |
Het |
| Cdc14b |
C |
T |
13: 64,353,338 (GRCm39) |
|
probably benign |
Het |
| Cenpu |
A |
G |
8: 47,015,615 (GRCm39) |
R177G |
probably benign |
Het |
| Chit1 |
C |
A |
1: 134,074,381 (GRCm39) |
Y229* |
probably null |
Het |
| Crybg2 |
C |
A |
4: 133,808,450 (GRCm39) |
S1350R |
probably damaging |
Het |
| Ctdspl2 |
A |
G |
2: 121,809,373 (GRCm39) |
|
probably null |
Het |
| Ctsr |
A |
T |
13: 61,308,345 (GRCm39) |
H266Q |
probably benign |
Het |
| Dynlt5 |
A |
G |
4: 102,849,766 (GRCm39) |
E63G |
possibly damaging |
Het |
| Efcab3 |
T |
G |
11: 104,746,386 (GRCm39) |
I2279S |
probably benign |
Het |
| Extl3 |
T |
A |
14: 65,313,584 (GRCm39) |
M533L |
probably benign |
Het |
| Fam162b |
C |
A |
10: 51,463,506 (GRCm39) |
|
probably null |
Het |
| Fbxo28 |
T |
C |
1: 182,157,454 (GRCm39) |
K121R |
probably damaging |
Het |
| Fsip2 |
A |
C |
2: 82,820,227 (GRCm39) |
E5320A |
possibly damaging |
Het |
| Galc |
A |
T |
12: 98,225,421 (GRCm39) |
D56E |
probably damaging |
Het |
| Gm5468 |
A |
G |
15: 25,414,481 (GRCm39) |
|
probably benign |
Het |
| Igkv5-43 |
A |
G |
6: 69,752,965 (GRCm39) |
V39A |
possibly damaging |
Het |
| Lemd2 |
G |
A |
17: 27,411,976 (GRCm39) |
Q439* |
probably null |
Het |
| Lgi1 |
A |
G |
19: 38,294,257 (GRCm39) |
N295S |
probably benign |
Het |
| Macc1 |
T |
G |
12: 119,409,785 (GRCm39) |
S184R |
probably damaging |
Het |
| Msmo1 |
A |
G |
8: 65,180,918 (GRCm39) |
|
probably benign |
Het |
| Muc5b |
C |
A |
7: 141,405,333 (GRCm39) |
R914S |
unknown |
Het |
| Or10d1 |
G |
A |
9: 39,483,776 (GRCm39) |
P260S |
possibly damaging |
Het |
| Or1e16 |
G |
A |
11: 73,286,299 (GRCm39) |
A183V |
probably benign |
Het |
| Or1e17 |
G |
A |
11: 73,831,635 (GRCm39) |
A188T |
possibly damaging |
Het |
| Or4k2 |
C |
A |
14: 50,424,135 (GRCm39) |
D180Y |
probably damaging |
Het |
| Or5b110-ps1 |
A |
G |
19: 13,260,290 (GRCm39) |
I44T |
probably benign |
Het |
| Plekha5 |
T |
C |
6: 140,525,179 (GRCm39) |
S14P |
probably benign |
Het |
| Pus10 |
A |
G |
11: 23,622,638 (GRCm39) |
K86R |
probably benign |
Het |
| Rab37 |
T |
C |
11: 115,051,132 (GRCm39) |
V147A |
probably benign |
Het |
| Sin3a |
T |
A |
9: 57,011,213 (GRCm39) |
I490N |
probably damaging |
Het |
| Slc27a4 |
A |
G |
2: 29,695,762 (GRCm39) |
D99G |
probably benign |
Het |
| Slc43a2 |
C |
T |
11: 75,459,206 (GRCm39) |
R413* |
probably null |
Het |
| Syna |
G |
T |
5: 134,588,466 (GRCm39) |
T161N |
probably benign |
Het |
| T |
A |
G |
17: 8,655,996 (GRCm39) |
D86G |
possibly damaging |
Het |
| Tanc1 |
G |
A |
2: 59,674,366 (GRCm39) |
E1817K |
possibly damaging |
Het |
| Tap2 |
C |
A |
17: 34,433,384 (GRCm39) |
Q516K |
possibly damaging |
Het |
| Tcaf1 |
A |
T |
6: 42,653,741 (GRCm39) |
D717E |
probably damaging |
Het |
| Tcf20 |
G |
T |
15: 82,736,187 (GRCm39) |
Q1755K |
possibly damaging |
Het |
| Top2b |
T |
G |
14: 16,409,189 (GRCm38) |
I777M |
probably damaging |
Het |
| Trpm7 |
G |
A |
2: 126,667,559 (GRCm39) |
P811S |
possibly damaging |
Het |
| Vmn2r59 |
A |
G |
7: 41,661,679 (GRCm39) |
V712A |
probably benign |
Het |
| Vwc2l |
T |
A |
1: 70,768,180 (GRCm39) |
D34E |
probably damaging |
Het |
| Wdr35 |
A |
G |
12: 9,077,632 (GRCm39) |
K1091E |
probably benign |
Het |
|
| Other mutations in Hk1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00493:Hk1
|
APN |
10 |
62,122,127 (GRCm39) |
nonsense |
probably null |
|
|
IGL01108:Hk1
|
APN |
10 |
62,132,487 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01810:Hk1
|
APN |
10 |
62,188,884 (GRCm39) |
missense |
probably benign |
0.13 |
|
IGL01950:Hk1
|
APN |
10 |
62,151,173 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02165:Hk1
|
APN |
10 |
62,117,667 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02227:Hk1
|
APN |
10 |
62,116,919 (GRCm39) |
splice site |
probably benign |
|
|
IGL02257:Hk1
|
APN |
10 |
62,107,422 (GRCm39) |
missense |
probably benign |
0.07 |
|
IGL02341:Hk1
|
APN |
10 |
62,120,159 (GRCm39) |
missense |
possibly damaging |
0.54 |
|
IGL02553:Hk1
|
APN |
10 |
62,131,552 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
IGL02623:Hk1
|
APN |
10 |
62,128,138 (GRCm39) |
missense |
probably benign |
0.21 |
|
IGL02700:Hk1
|
APN |
10 |
62,120,590 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02863:Hk1
|
APN |
10 |
62,131,534 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
IGL03002:Hk1
|
APN |
10 |
62,107,578 (GRCm39) |
missense |
probably damaging |
1.00 |
|
BB009:Hk1
|
UTSW |
10 |
62,151,299 (GRCm39) |
missense |
probably damaging |
1.00 |
|
BB019:Hk1
|
UTSW |
10 |
62,151,299 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0029:Hk1
|
UTSW |
10 |
62,151,173 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0436:Hk1
|
UTSW |
10 |
62,135,054 (GRCm39) |
splice site |
probably benign |
|
|
R0853:Hk1
|
UTSW |
10 |
62,107,495 (GRCm39) |
nonsense |
probably null |
|
|
R1422:Hk1
|
UTSW |
10 |
62,131,873 (GRCm39) |
missense |
probably null |
0.98 |
|
R1531:Hk1
|
UTSW |
10 |
62,120,563 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1760:Hk1
|
UTSW |
10 |
62,117,678 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2064:Hk1
|
UTSW |
10 |
62,122,315 (GRCm39) |
missense |
probably benign |
0.03 |
|
R3236:Hk1
|
UTSW |
10 |
62,131,798 (GRCm39) |
splice site |
probably null |
|
|
R3788:Hk1
|
UTSW |
10 |
62,111,467 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R3977:Hk1
|
UTSW |
10 |
62,126,098 (GRCm39) |
missense |
probably benign |
0.10 |
|
R4373:Hk1
|
UTSW |
10 |
62,151,319 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4374:Hk1
|
UTSW |
10 |
62,151,319 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4377:Hk1
|
UTSW |
10 |
62,151,319 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4435:Hk1
|
UTSW |
10 |
62,111,623 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4609:Hk1
|
UTSW |
10 |
62,194,194 (GRCm39) |
utr 5 prime |
probably benign |
|
|
R4648:Hk1
|
UTSW |
10 |
62,140,558 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4864:Hk1
|
UTSW |
10 |
62,178,318 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4934:Hk1
|
UTSW |
10 |
62,194,165 (GRCm39) |
utr 5 prime |
probably benign |
|
|
R5110:Hk1
|
UTSW |
10 |
62,122,430 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5352:Hk1
|
UTSW |
10 |
62,140,549 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5569:Hk1
|
UTSW |
10 |
62,122,220 (GRCm39) |
missense |
probably benign |
0.35 |
|
R5609:Hk1
|
UTSW |
10 |
62,178,330 (GRCm39) |
missense |
probably benign |
0.30 |
|
R5647:Hk1
|
UTSW |
10 |
62,111,523 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5750:Hk1
|
UTSW |
10 |
62,110,245 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R5770:Hk1
|
UTSW |
10 |
62,122,228 (GRCm39) |
missense |
probably benign |
|
|
R5832:Hk1
|
UTSW |
10 |
62,128,144 (GRCm39) |
missense |
probably benign |
0.17 |
|
R5905:Hk1
|
UTSW |
10 |
62,188,837 (GRCm39) |
missense |
probably null |
0.82 |
|
R5933:Hk1
|
UTSW |
10 |
62,105,773 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6028:Hk1
|
UTSW |
10 |
62,188,837 (GRCm39) |
missense |
probably null |
0.82 |
|
R6314:Hk1
|
UTSW |
10 |
62,128,223 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R6372:Hk1
|
UTSW |
10 |
62,127,757 (GRCm39) |
missense |
probably benign |
|
|
R6801:Hk1
|
UTSW |
10 |
62,116,910 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6838:Hk1
|
UTSW |
10 |
62,107,437 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R7045:Hk1
|
UTSW |
10 |
62,122,349 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7420:Hk1
|
UTSW |
10 |
62,105,761 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7491:Hk1
|
UTSW |
10 |
62,131,524 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7527:Hk1
|
UTSW |
10 |
62,140,561 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7561:Hk1
|
UTSW |
10 |
62,116,807 (GRCm39) |
splice site |
probably null |
|
|
R7932:Hk1
|
UTSW |
10 |
62,151,299 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8031:Hk1
|
UTSW |
10 |
62,132,478 (GRCm39) |
missense |
probably benign |
0.15 |
|
R8128:Hk1
|
UTSW |
10 |
62,117,622 (GRCm39) |
missense |
probably benign |
|
|
R8204:Hk1
|
UTSW |
10 |
62,132,523 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8294:Hk1
|
UTSW |
10 |
62,131,624 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8685:Hk1
|
UTSW |
10 |
62,132,453 (GRCm39) |
splice site |
probably benign |
|
|
R8865:Hk1
|
UTSW |
10 |
62,151,294 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9015:Hk1
|
UTSW |
10 |
62,128,118 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R9022:Hk1
|
UTSW |
10 |
62,105,768 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9063:Hk1
|
UTSW |
10 |
62,122,429 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9404:Hk1
|
UTSW |
10 |
62,131,859 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
X0018:Hk1
|
UTSW |
10 |
62,111,485 (GRCm39) |
missense |
probably benign |
0.02 |
|
X0063:Hk1
|
UTSW |
10 |
62,111,483 (GRCm39) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TCCCAAAACTAGGAGGCAGG -3'
(R):5'- AAGACTCTCTGCCAACTCTCTTAG -3'
Sequencing Primer
(F):5'- CTAGGAGGCAGGGGGTCAC -3'
(R):5'- TGCCAACTCTCTTAGTTTCTGAG -3'
|
| Posted On |
2018-02-27 |
Incidental Mutation