Incidental Mutation 'R6198:Grp'
ID503075
Institutional Source Beutler Lab
Gene Symbol Grp
Ensembl Gene ENSMUSG00000024517
Gene Namegastrin releasing peptide
SynonymsBLP
MMRRC Submission 044338-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R6198 (G1)
Quality Score225.009
Status Validated
Chromosome18
Chromosomal Location65872820-65886579 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to T at 65879986 bp
ZygosityHeterozygous
Amino Acid Change Glutamine to Histidine at position 74 (Q74H)
Ref Sequence ENSEMBL: ENSMUSP00000133437 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025395] [ENSMUST00000173530] [ENSMUST00000173985]
Predicted Effect possibly damaging
Transcript: ENSMUST00000025395
AA Change: Q74H

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000025395
Gene: ENSMUSG00000024517
AA Change: Q74H

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
Pfam:Bombesin 43 56 9.1e-10 PFAM
low complexity region 82 95 N/A INTRINSIC
low complexity region 98 112 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000173530
AA Change: Q74H

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000139014
Gene: ENSMUSG00000024517
AA Change: Q74H

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
Pfam:Bombesin 43 56 1.2e-11 PFAM
low complexity region 82 95 N/A INTRINSIC
low complexity region 98 112 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000173985
AA Change: Q74H

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000133437
Gene: ENSMUSG00000024517
AA Change: Q74H

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
Pfam:Bombesin 43 56 3.5e-11 PFAM
low complexity region 82 95 N/A INTRINSIC
low complexity region 98 112 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 99% (68/69)
MGI Phenotype FUNCTION: This gene encodes a neuropeptide hormone that affects various biological processes such as neuroendocrine regulation, gastrointestinal secretion, nociception, cell proliferation and inflammation. The encoded protein undergoes proteolytic processing to generate multiple mature peptides with biological activity. [provided by RefSeq, Aug 2015]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit attenuated scratching response to chloroquine, SLIGRL and BAM8-22. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700006A11Rik A T 3: 124,416,850 probably null Het
1700061G19Rik A G 17: 56,882,679 S265G probably damaging Het
2610318N02Rik A G 16: 17,118,369 S164P probably damaging Het
Acyp2 C T 11: 30,506,354 E98K possibly damaging Het
Adam19 A C 11: 46,121,502 N275T probably damaging Het
Adgrg7 T A 16: 56,777,193 T84S possibly damaging Het
Asl C T 5: 130,018,916 V70I probably benign Het
Atp2c1 A G 9: 105,521,072 S26P probably benign Het
Bcas3 A T 11: 85,509,435 D410V probably damaging Het
Cc2d1b C T 4: 108,633,225 R825W probably damaging Het
Ccdc15 A C 9: 37,314,285 probably null Het
Cfh A G 1: 140,105,440 S789P probably damaging Het
Cltc T C 11: 86,720,362 N561S probably benign Het
Cnst A G 1: 179,592,865 Q187R probably damaging Het
Csmd3 T C 15: 48,313,877 T422A probably benign Het
Cyp2c55 A T 19: 39,007,121 R26* probably null Het
Dgkb A T 12: 38,173,823 M414L probably benign Het
Dgkd A G 1: 87,924,208 D444G probably damaging Het
Dnmt3c T C 2: 153,720,009 V544A noncoding transcript Het
Dus3l A T 17: 56,767,858 T327S possibly damaging Het
Elf2 A T 3: 51,277,249 L5Q probably damaging Het
Fam69b A G 2: 26,635,698 K215E probably damaging Het
Fxyd6 A G 9: 45,390,670 Y30C probably damaging Het
Gcc2 A T 10: 58,292,590 T1375S probably benign Het
Git2 A T 5: 114,745,495 Y393* probably null Het
Gm14403 AAACCCTA AA 2: 177,509,655 probably benign Het
Gm9573 T A 17: 35,620,916 probably benign Het
Golgb1 T C 16: 36,893,395 L246P probably damaging Het
Ifi203 A T 1: 173,924,082 M391K probably damaging Het
Itih2 A G 2: 10,098,541 Y712H probably benign Het
Kdm5a T A 6: 120,438,997 V1626E probably benign Het
Klhl40 A G 9: 121,778,767 Y331C probably damaging Het
Kprp T A 3: 92,824,687 Y352F probably damaging Het
Lama2 G T 10: 27,188,022 H1286Q probably damaging Het
Lgi4 T A 7: 31,069,122 probably null Het
Lrrc47 A T 4: 154,015,672 N235I probably damaging Het
Lrrc74b T A 16: 17,548,786 I308F probably damaging Het
Map7d1 T A 4: 126,241,843 K135M probably damaging Het
March5 A G 19: 37,210,741 R36G probably damaging Het
Mtx3 C A 13: 92,852,851 P299Q probably benign Het
Ncapd2 C A 6: 125,179,323 E500* probably null Het
Nckap5l T C 15: 99,425,988 K878R probably damaging Het
Nphs1 T C 7: 30,467,915 I756T probably damaging Het
Olfm4 T G 14: 80,000,373 S17A probably benign Het
Olfr1270 A T 2: 90,149,438 D189E probably damaging Het
Olfr344 A G 2: 36,568,951 M118V probably damaging Het
Pak1ip1 G T 13: 41,001,410 Q27H probably benign Het
Piezo2 A T 18: 63,157,210 C159* probably null Het
Pkn2 G T 3: 142,810,404 T538K probably benign Het
Ppp3cc A T 14: 70,247,611 M198K probably benign Het
Rrm1 T G 7: 102,446,729 probably null Het
Setd3 T C 12: 108,165,168 K7E possibly damaging Het
Shc1 A G 3: 89,422,107 K86R probably benign Het
Slc16a7 T C 10: 125,228,215 T418A probably benign Het
Spocd1 C T 4: 129,955,415 P676S probably damaging Het
Spp1 A G 5: 104,439,508 probably null Het
Syne1 A G 10: 5,302,269 Y2462H probably damaging Het
Tiam2 T C 17: 3,414,121 S42P probably benign Het
Tmem63b C T 17: 45,661,516 V722I probably benign Het
Ubqln1 A G 13: 58,196,590 S130P probably benign Het
Usp34 G T 11: 23,484,127 L3215F probably damaging Het
Uvssa A G 5: 33,409,510 Y517C probably damaging Het
Vps13d A T 4: 145,148,990 F1649Y probably benign Het
Zfp236 A G 18: 82,657,153 S405P probably damaging Het
Zfp9 T C 6: 118,477,321 M1V probably null Het
Zfp946 A T 17: 22,454,915 S217C probably damaging Het
Zfp970 T A 2: 177,475,460 C276S probably damaging Het
Zswim5 T A 4: 116,878,007 F183Y probably benign Het
Other mutations in Grp
AlleleSourceChrCoordTypePredicted EffectPPH Score
PIT4362001:Grp UTSW 18 65886226 missense probably benign 0.18
R0584:Grp UTSW 18 65873695 missense possibly damaging 0.79
R1144:Grp UTSW 18 65879970 missense probably damaging 0.99
R4865:Grp UTSW 18 65879970 missense probably damaging 1.00
R5085:Grp UTSW 18 65880159 missense probably benign
R6084:Grp UTSW 18 65879937 missense probably damaging 0.99
R6325:Grp UTSW 18 65873753 critical splice donor site probably null
Predicted Primers PCR Primer
(F):5'- GCCCATGTGTTGTGTATAGCCC -3'
(R):5'- CACCTTAGCGGTTTGAACGTC -3'

Sequencing Primer
(F):5'- ATGTGTTGTGTATAGCCCAACTC -3'
(R):5'- ACGTCGTTAAAGTAGCTGCC -3'
Posted On2018-02-27