Mutagenetix > Incidental Mutation

Incidental Mutation 'R6199:Map2'

503080

Institutional Source

Beutler Lab

Gene Symbol

Map2

Ensembl Gene

ENSMUSG00000015222

Gene Name

microtubule-associated protein 2

Synonyms

G1-397-34, MAP-2, Mtap2, repro4

MMRRC Submission

044339-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.679) question?

Stock #

R6199 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66214432-66481742 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 66464637 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Alanine at position 1676 (S1676A)

Ref Sequence

ENSEMBL: ENSMUSP00000109646 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024639] [ENSMUST00000077355] [ENSMUST00000114012] [ENSMUST00000114013] [ENSMUST00000114015] [ENSMUST00000114017] [ENSMUST00000114018] [ENSMUST00000172886] [ENSMUST00000173778] [ENSMUST00000173800]

AlphaFold

P20357

Predicted Effect

probably damaging
Transcript: ENSMUST00000024639
AA Change: S315A

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000024639
Gene: ENSMUSG00000015222
AA Change: S315A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000077355
AA Change: S315A

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000076577
Gene: ENSMUSG00000015222
AA Change: S315A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114012
AA Change: S314A

PolyPhen 2 Score 0.578 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000109645
Gene: ENSMUSG00000015222
AA Change: S314A

Domain	Start	End	E-Value	Type
low complexity region	133	140	N/A	INTRINSIC
low complexity region	181	195	N/A	INTRINSIC
low complexity region	205	221	N/A	INTRINSIC
low complexity region	228	250	N/A	INTRINSIC
Pfam:Tubulin-binding	299	330	2.1e-18	PFAM
Pfam:Tubulin-binding	331	361	9.1e-20	PFAM
Pfam:Tubulin-binding	362	393	1.7e-17	PFAM
low complexity region	421	434	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114013
AA Change: S1676A

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000109646
Gene: ENSMUSG00000015222
AA Change: S1676A

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	86	103	1.2e-5	PFAM
low complexity region	120	141	N/A	INTRINSIC
Pfam:MAP2_projctn	376	1510	N/A	PFAM
low complexity region	1543	1557	N/A	INTRINSIC
low complexity region	1567	1583	N/A	INTRINSIC
low complexity region	1590	1612	N/A	INTRINSIC
Pfam:Tubulin-binding	1662	1692	1.7e-13	PFAM
Pfam:Tubulin-binding	1693	1723	5.8e-18	PFAM
Pfam:Tubulin-binding	1724	1755	5.9e-18	PFAM
low complexity region	1783	1796	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114015
AA Change: S315A

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000109648
Gene: ENSMUSG00000015222
AA Change: S315A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114017
AA Change: S315A

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000109650
Gene: ENSMUSG00000015222
AA Change: S315A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114018
AA Change: S315A

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000109651
Gene: ENSMUSG00000015222
AA Change: S315A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000172886
AA Change: S245A

PolyPhen 2 Score 0.970 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000133446
Gene: ENSMUSG00000015222
AA Change: S245A

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	107	3.9e-54	PFAM
low complexity region	112	126	N/A	INTRINSIC
low complexity region	136	152	N/A	INTRINSIC
low complexity region	159	181	N/A	INTRINSIC
Pfam:Tubulin-binding	230	261	1.4e-18	PFAM
Pfam:Tubulin-binding	262	292	1.4e-19	PFAM
Pfam:Tubulin-binding	293	323	6e-20	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000173778
AA Change: S290A

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000134651
Gene: ENSMUSG00000015222
AA Change: S290A

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	124	4.8e-84	PFAM
low complexity region	157	171	N/A	INTRINSIC
low complexity region	181	197	N/A	INTRINSIC
low complexity region	204	226	N/A	INTRINSIC
Pfam:Tubulin-binding	275	306	1.6e-18	PFAM
Pfam:Tubulin-binding	307	337	1.6e-19	PFAM
Pfam:Tubulin-binding	338	368	7e-20	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000173800
AA Change: S188A

PolyPhen 2 Score 0.941 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000134518
Gene: ENSMUSG00000015222
AA Change: S188A

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	23	2.2e-11	PFAM
low complexity region	55	69	N/A	INTRINSIC
low complexity region	79	95	N/A	INTRINSIC
low complexity region	102	124	N/A	INTRINSIC
Pfam:Tubulin-binding	173	204	8.7e-19	PFAM
Pfam:Tubulin-binding	205	235	3.8e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133522

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151423

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

100% (53/53)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit altered contextual memory. Mice homozygous for another knock-out allele display decreased body weight, altered microtubule density and organization in Purkinje cell dendrites, and reduced dendritic length inhippocampal neurons. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adck1	A	T	12: 88,407,887 (GRCm39)	D206V	possibly damaging	Het
Ank2	T	A	3: 126,797,655 (GRCm39)	D685V	probably damaging	Het
Baz2b	A	G	2: 59,809,019 (GRCm39)	S77P	probably benign	Het
Ccdc194	T	C	8: 71,978,109 (GRCm39)	N83D	probably benign	Het
Ceacam5	A	T	7: 17,448,810 (GRCm39)	T59S	probably benign	Het
Cemip2	G	A	19: 21,822,186 (GRCm39)	G1194S	probably benign	Het
Ces1e	A	C	8: 93,944,163 (GRCm39)	F218L	probably damaging	Het
Cilk1	T	C	9: 78,071,921 (GRCm39)	V531A	probably benign	Het
Cps1	TGTCCATTGGTC	TGTC	1: 67,201,774 (GRCm39)		probably null	Het
Dab1	C	T	4: 104,588,948 (GRCm39)	A524V	probably benign	Het
Eftud2	A	T	11: 102,730,883 (GRCm39)	V843E	probably damaging	Het
Fuca2	G	T	10: 13,381,783 (GRCm39)	W232L	probably damaging	Het
Gdf7	T	C	12: 8,348,832 (GRCm39)	D155G	unknown	Het
Ggcx	G	T	6: 72,407,122 (GRCm39)	V753F	possibly damaging	Het
Ghrhr	A	T	6: 55,356,173 (GRCm39)	T89S	probably benign	Het
Gpr151	T	C	18: 42,711,619 (GRCm39)	K353R	probably benign	Het
Gpr75	T	C	11: 30,841,527 (GRCm39)	L144P	probably damaging	Het
Gsdmc2	A	G	15: 63,696,962 (GRCm39)	I403T	probably benign	Het
H2-M1	A	G	17: 36,982,059 (GRCm39)	S181P	probably benign	Het
Igsf5	C	T	16: 96,222,939 (GRCm39)	S61L	possibly damaging	Het
Insc	A	T	7: 114,390,401 (GRCm39)		probably null	Het
Izumo4	G	A	10: 80,538,707 (GRCm39)	G53D	probably damaging	Het
Ksr1	G	A	11: 78,911,267 (GRCm39)	P693S	possibly damaging	Het
Lgals4	G	A	7: 28,535,317 (GRCm39)	R27H	probably damaging	Het
Lpcat2b	A	G	5: 107,581,171 (GRCm39)	R167G	probably benign	Het
Man1a	C	T	10: 53,890,552 (GRCm39)	V288I	possibly damaging	Het
Mbl1	G	T	14: 40,875,572 (GRCm39)	V9F	unknown	Het
Mrgprb8	T	A	7: 48,039,051 (GRCm39)	C241S	probably benign	Het
Mrpl2	T	C	17: 46,960,012 (GRCm39)	L227P	probably damaging	Het
Mthfd1	T	A	12: 76,335,685 (GRCm39)	V253E	probably damaging	Het
Mthfd1	A	G	12: 76,350,454 (GRCm39)	H464R	probably damaging	Het
Mug2	T	A	6: 122,024,398 (GRCm39)	M490K	probably benign	Het
Naip6	C	T	13: 100,437,108 (GRCm39)	A472T	probably benign	Het
Notch1	A	G	2: 26,359,911 (GRCm39)	V1268A	probably damaging	Het
Or6b2b	A	T	1: 92,419,264 (GRCm39)	I71N	possibly damaging	Het
Or8b12c	C	A	9: 37,716,177 (GRCm39)		probably null	Het
Pgm1	A	T	4: 99,836,151 (GRCm39)	I412F	probably damaging	Het
Plaur	A	T	7: 24,164,628 (GRCm39)	Q44L	possibly damaging	Het
Ppara	T	C	15: 85,671,434 (GRCm39)	Y112H	probably damaging	Het
Ppm1h	G	A	10: 122,756,644 (GRCm39)	V430M	probably damaging	Het
Prpf40a	T	C	2: 53,047,927 (GRCm39)	M197V	probably benign	Het
Prph	A	G	15: 98,954,713 (GRCm39)	T35A	probably benign	Het
Prrc2c	C	T	1: 162,510,085 (GRCm39)	G780S	probably damaging	Het
Ptchd3	T	A	11: 121,721,908 (GRCm39)	N260K	probably benign	Het
Ptprz1	A	G	6: 23,002,470 (GRCm39)	D1520G	probably benign	Het
Samd9l	T	A	6: 3,376,686 (GRCm39)	I192L	probably benign	Het
Slc39a10	T	C	1: 46,874,993 (GRCm39)	D103G	probably damaging	Het
Smndc1	G	A	19: 53,372,063 (GRCm39)	T117M	probably benign	Het
Tesk2	A	G	4: 116,649,367 (GRCm39)	D159G	probably damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Vmn2r108	A	T	17: 20,682,644 (GRCm39)	N853K	probably benign	Het
Wdfy3	C	T	5: 102,020,831 (GRCm39)	R2491Q	possibly damaging	Het

Other mutations in Map2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Map2	APN	1	66,464,490 (GRCm39)	missense	probably damaging	1.00
IGL02135:Map2	APN	1	66,419,920 (GRCm39)	nonsense	probably null
IGL02526:Map2	APN	1	66,419,876 (GRCm39)	missense	possibly damaging	0.94
Annas	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
calliope	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
carthage	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
costas	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
Jacobin	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
Nectar	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
ruby_throat	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
Rufous	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
Speckled	UTSW	1	66,451,886 (GRCm39)	nonsense	probably null
Sunbird	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
swift	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
E0370:Map2	UTSW	1	66,455,883 (GRCm39)	unclassified	probably benign
PIT4362001:Map2	UTSW	1	66,451,677 (GRCm39)	missense	probably benign	0.05
R0067:Map2	UTSW	1	66,452,322 (GRCm39)	missense	probably benign	0.04
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0268:Map2	UTSW	1	66,419,881 (GRCm39)	nonsense	probably null
R0302:Map2	UTSW	1	66,453,987 (GRCm39)	missense	probably benign	0.15
R0305:Map2	UTSW	1	66,452,253 (GRCm39)	missense	probably benign	0.00
R0409:Map2	UTSW	1	66,472,739 (GRCm39)	missense	probably damaging	1.00
R0561:Map2	UTSW	1	66,464,656 (GRCm39)	missense	probably damaging	1.00
R0674:Map2	UTSW	1	66,452,361 (GRCm39)	missense	probably damaging	1.00
R0738:Map2	UTSW	1	66,464,348 (GRCm39)	splice site	probably benign
R0893:Map2	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
R1305:Map2	UTSW	1	66,464,554 (GRCm39)	missense	probably damaging	1.00
R1534:Map2	UTSW	1	66,452,339 (GRCm39)	missense	probably benign	0.33
R1632:Map2	UTSW	1	66,454,245 (GRCm39)	missense	possibly damaging	0.60
R1682:Map2	UTSW	1	66,454,781 (GRCm39)	splice site	probably null
R1774:Map2	UTSW	1	66,453,233 (GRCm39)	missense	probably damaging	1.00
R2014:Map2	UTSW	1	66,455,295 (GRCm39)	missense	possibly damaging	0.55
R2017:Map2	UTSW	1	66,451,958 (GRCm39)	missense	probably damaging	1.00
R2050:Map2	UTSW	1	66,453,473 (GRCm39)	missense	probably damaging	0.98
R2093:Map2	UTSW	1	66,438,599 (GRCm39)	missense	probably damaging	1.00
R2214:Map2	UTSW	1	66,459,345 (GRCm39)	missense	probably damaging	0.99
R2284:Map2	UTSW	1	66,453,227 (GRCm39)	missense	probably damaging	1.00
R3011:Map2	UTSW	1	66,453,771 (GRCm39)	missense	probably damaging	1.00
R3105:Map2	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
R3708:Map2	UTSW	1	66,455,714 (GRCm39)	unclassified	probably benign
R3709:Map2	UTSW	1	66,455,015 (GRCm39)	nonsense	probably null
R3729:Map2	UTSW	1	66,451,605 (GRCm39)	missense	possibly damaging	0.80
R3760:Map2	UTSW	1	66,478,077 (GRCm39)	missense	probably damaging	1.00
R3788:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R3789:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R4003:Map2	UTSW	1	66,454,899 (GRCm39)	missense	probably damaging	1.00
R4120:Map2	UTSW	1	66,455,063 (GRCm39)	missense	probably damaging	1.00
R4172:Map2	UTSW	1	66,452,759 (GRCm39)	missense	possibly damaging	0.89
R4198:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4200:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4205:Map2	UTSW	1	66,464,449 (GRCm39)	missense	probably damaging	1.00
R4613:Map2	UTSW	1	66,464,628 (GRCm39)	missense	probably damaging	1.00
R4700:Map2	UTSW	1	66,449,796 (GRCm39)	missense	probably damaging	0.96
R4974:Map2	UTSW	1	66,452,664 (GRCm39)	missense	probably benign	0.15
R5007:Map2	UTSW	1	66,452,448 (GRCm39)	missense	possibly damaging	0.86
R5039:Map2	UTSW	1	66,477,955 (GRCm39)	missense	probably damaging	1.00
R5237:Map2	UTSW	1	66,478,169 (GRCm39)	unclassified	probably benign
R5313:Map2	UTSW	1	66,464,538 (GRCm39)	missense	probably damaging	1.00
R5455:Map2	UTSW	1	66,438,550 (GRCm39)	missense	probably damaging	1.00
R5490:Map2	UTSW	1	66,452,292 (GRCm39)	missense	probably damaging	1.00
R5517:Map2	UTSW	1	66,454,415 (GRCm39)	missense	probably benign	0.00
R5532:Map2	UTSW	1	66,453,779 (GRCm39)	missense	probably damaging	1.00
R5583:Map2	UTSW	1	66,455,196 (GRCm39)	missense	probably damaging	1.00
R5764:Map2	UTSW	1	66,454,034 (GRCm39)	missense	probably damaging	0.99
R5996:Map2	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
R6058:Map2	UTSW	1	66,454,573 (GRCm39)	missense	probably benign	0.05
R6208:Map2	UTSW	1	66,470,749 (GRCm39)	missense	probably damaging	1.00
R6276:Map2	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
R6378:Map2	UTSW	1	66,454,488 (GRCm39)	missense	probably damaging	1.00
R6424:Map2	UTSW	1	66,453,946 (GRCm39)	missense	possibly damaging	0.67
R6743:Map2	UTSW	1	66,454,766 (GRCm39)	missense	probably benign	0.04
R6837:Map2	UTSW	1	66,453,731 (GRCm39)	missense	probably damaging	1.00
R6901:Map2	UTSW	1	66,460,932 (GRCm39)	missense	possibly damaging	0.94
R6984:Map2	UTSW	1	66,454,395 (GRCm39)	missense	possibly damaging	0.90
R6989:Map2	UTSW	1	66,454,065 (GRCm39)	missense	probably benign	0.00
R7001:Map2	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
R7055:Map2	UTSW	1	66,455,983 (GRCm39)	missense	probably damaging	1.00
R7094:Map2	UTSW	1	66,451,886 (GRCm39)	nonsense	probably null
R7106:Map2	UTSW	1	66,449,903 (GRCm39)	missense	possibly damaging	0.92
R7182:Map2	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
R7235:Map2	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
R7424:Map2	UTSW	1	66,453,983 (GRCm39)	missense	possibly damaging	0.69
R7473:Map2	UTSW	1	66,454,617 (GRCm39)	missense	probably damaging	1.00
R7642:Map2	UTSW	1	66,452,466 (GRCm39)	missense	probably benign	0.01
R7660:Map2	UTSW	1	66,453,536 (GRCm39)	missense	probably damaging	1.00
R7673:Map2	UTSW	1	66,452,933 (GRCm39)	missense	probably benign	0.03
R7768:Map2	UTSW	1	66,453,642 (GRCm39)	missense	possibly damaging	0.94
R7796:Map2	UTSW	1	66,455,654 (GRCm39)	splice site	probably null
R7834:Map2	UTSW	1	66,455,647 (GRCm39)	missense	probably damaging	1.00
R7842:Map2	UTSW	1	66,455,570 (GRCm39)	missense	probably benign
R7955:Map2	UTSW	1	66,452,875 (GRCm39)	missense	probably damaging	1.00
R8056:Map2	UTSW	1	66,454,779 (GRCm39)	missense	probably damaging	0.99
R8118:Map2	UTSW	1	66,464,550 (GRCm39)	missense	probably damaging	1.00
R8135:Map2	UTSW	1	66,452,828 (GRCm39)	missense	probably damaging	1.00
R8152:Map2	UTSW	1	66,453,902 (GRCm39)	missense	probably benign	0.00
R8223:Map2	UTSW	1	66,464,649 (GRCm39)	missense	probably damaging	1.00
R8329:Map2	UTSW	1	66,454,272 (GRCm39)	missense	probably benign	0.01
R8344:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8475:Map2	UTSW	1	66,453,164 (GRCm39)	missense	probably damaging	1.00
R8548:Map2	UTSW	1	66,452,499 (GRCm39)	missense	probably damaging	1.00
R8680:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8713:Map2	UTSW	1	66,453,781 (GRCm39)	missense	probably damaging	1.00
R8745:Map2	UTSW	1	66,452,556 (GRCm39)	missense	probably benign	0.00
R8786:Map2	UTSW	1	66,472,755 (GRCm39)	critical splice donor site	probably benign
R8790:Map2	UTSW	1	66,477,997 (GRCm39)	missense	probably damaging	1.00
R8874:Map2	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
R8887:Map2	UTSW	1	66,454,758 (GRCm39)	missense	possibly damaging	0.48
R8948:Map2	UTSW	1	66,419,684 (GRCm39)	missense	probably damaging	1.00
R8950:Map2	UTSW	1	66,419,684 (GRCm39)	missense	probably damaging	1.00
R8998:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R8999:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R9072:Map2	UTSW	1	66,453,312 (GRCm39)	missense	probably damaging	1.00
R9088:Map2	UTSW	1	66,453,773 (GRCm39)	missense	probably damaging	1.00
R9089:Map2	UTSW	1	66,452,098 (GRCm39)	missense	probably benign	0.09
R9106:Map2	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
R9112:Map2	UTSW	1	66,472,723 (GRCm39)	nonsense	probably null
R9120:Map2	UTSW	1	66,453,218 (GRCm39)	missense	probably damaging	1.00
R9161:Map2	UTSW	1	66,477,503 (GRCm39)	missense	possibly damaging	0.65
R9464:Map2	UTSW	1	66,454,497 (GRCm39)	missense	probably damaging	1.00
R9589:Map2	UTSW	1	66,449,753 (GRCm39)	missense	probably benign
V8831:Map2	UTSW	1	66,455,004 (GRCm39)	missense	probably damaging	1.00
Z1177:Map2	UTSW	1	66,477,520 (GRCm39)	missense	probably damaging	0.96
Z1177:Map2	UTSW	1	66,419,839 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGGATCAACTGCAATCACCC -3'
(R):5'- GGTGACCCATGACCCTGTAAAC -3'

Sequencing Primer
(F):5'- ACTCTTCACGTACCCCAGG -3'
(R):5'- CCATGACCCTGTAAACATCTCTG -3'

Posted On

2018-02-27