Mutagenetix > Incidental Mutation

Incidental Mutation 'R6209:Svep1'

503316

Institutional Source

Beutler Lab

Gene Symbol

Svep1

Ensembl Gene

ENSMUSG00000028369

Gene Name

sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1

Synonyms

1110021D17Rik, Polydom, D430029O09Rik, 4833413O10Rik

MMRRC Submission

044343-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6209 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

58042796-58206596 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 58128869 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 609 (F609L)

Ref Sequence

ENSEMBL: ENSMUSP00000045856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042850]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000042850
AA Change: F609L

PolyPhen 2 Score 0.317 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000045856
Gene: ENSMUSG00000028369
AA Change: F609L

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	51	60	N/A	INTRINSIC
VWA	82	261	2.18e-32	SMART
Pfam:GCC2_GCC3	311	361	3.4e-14	PFAM
CCP	379	434	3.62e-8	SMART
CCP	439	494	1.78e-16	SMART
CCP	499	559	2.13e-5	SMART
Pfam:HYR	560	642	1.7e-20	PFAM
Pfam:HYR	643	722	4.6e-15	PFAM
CCP	727	787	3.59e-1	SMART
low complexity region	862	873	N/A	INTRINSIC
Pfam:GCC2_GCC3	1004	1051	3.2e-16	PFAM
Pfam:GCC2_GCC3	1058	1105	5.4e-19	PFAM
Pfam:GCC2_GCC3	1112	1159	7.7e-19	PFAM
EGF	1195	1228	3.12e-7	SMART
EGF_CA	1230	1266	3.93e-13	SMART
EGF_CA	1268	1304	8.3e-12	SMART
EGF_CA	1306	1342	4.59e-14	SMART
EGF_CA	1344	1380	8.69e-15	SMART
EGF_CA	1382	1418	3.42e-13	SMART
Pfam:Pentaxin	1429	1622	1.8e-28	PFAM
Pfam:Laminin_G_3	1432	1589	1.1e-20	PFAM
CCP	1630	1684	1.71e-9	SMART
CCP	1689	1742	2.31e-15	SMART
EGF_CA	1744	1783	5.23e-9	SMART
CCP	1788	1841	4.62e-15	SMART
CCP	1846	1899	8.29e-17	SMART
CCP	1904	1957	1.1e-12	SMART
CCP	1962	2015	5.6e-14	SMART
CCP	2020	2077	4.15e-8	SMART
CCP	2082	2140	8.11e-11	SMART
CCP	2145	2198	4.38e-16	SMART
CCP	2203	2258	1.69e-8	SMART
CCP	2263	2317	1.42e-15	SMART
CCP	2322	2375	3.1e-7	SMART
CCP	2380	2434	4.55e-14	SMART
CCP	2439	2492	6.95e-10	SMART
CCP	2497	2550	8.88e-17	SMART
CCP	2555	2607	1.7e-13	SMART
CCP	2651	2709	1.02e-7	SMART
CCP	2714	2767	9.6e-13	SMART
CCP	2772	2825	3.64e-13	SMART
CCP	2830	2883	6.63e-16	SMART
CCP	2888	2941	2.76e-13	SMART
CCP	2946	2999	4.41e-12	SMART
CCP	3004	3055	4.25e-5	SMART
CCP	3060	3113	5.15e-13	SMART
CCP	3118	3172	2.11e-9	SMART
CCP	3177	3232	1.02e-7	SMART
CCP	3237	3290	6.19e-16	SMART
CCP	3295	3348	5.35e-11	SMART
CCP	3353	3407	8.43e-9	SMART
CCP	3412	3464	2.44e-14	SMART
EGF	3467	3496	1.28e-3	SMART
EGF	3499	3528	1.15e-5	SMART
EGF	3531	3560	2.85e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit complete preweaning lethality, edema, abnormal skin coloration, thick epidermis, acanthosis, and tail/limb abnormalities. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aff3	T	A	1: 38,232,670 (GRCm39)	M990L	probably benign	Het
Agl	A	T	3: 116,578,845 (GRCm39)	Y429*	probably null	Het
Ahnak	A	T	19: 8,989,930 (GRCm39)	K3738M	probably damaging	Het
Amn	G	T	12: 111,241,845 (GRCm39)	V304L	probably damaging	Het
Ap3s1	G	A	18: 46,912,318 (GRCm39)	V113I	probably benign	Het
Arhgef28	A	T	13: 98,065,917 (GRCm39)		probably null	Het
Atad2	A	G	15: 57,981,811 (GRCm39)	S18P	probably damaging	Het
Bhmt1b	C	T	18: 87,775,341 (GRCm39)	A288V	probably damaging	Het
C4b	T	A	17: 34,960,061 (GRCm39)	E305V	possibly damaging	Het
Caskin1	T	C	17: 24,726,095 (GRCm39)	S1401P	possibly damaging	Het
Cblc	A	T	7: 19,519,230 (GRCm39)	V366D	possibly damaging	Het
Cdk17	A	T	10: 93,044,093 (GRCm39)	T11S	probably benign	Het
Cenpc1	A	G	5: 86,181,509 (GRCm39)	S619P	probably benign	Het
Clec4d	T	G	6: 123,247,488 (GRCm39)		probably null	Het
Disp2	T	C	2: 118,617,402 (GRCm39)	L132P	probably damaging	Het
Dync2h1	T	C	9: 7,165,677 (GRCm39)	K528R	probably benign	Het
Fbxw26	A	G	9: 109,547,033 (GRCm39)	I464T	possibly damaging	Het
Gaa	C	A	11: 119,171,997 (GRCm39)	A700D	probably benign	Het
Gabbr2	A	G	4: 46,804,069 (GRCm39)	V262A	probably damaging	Het
Galnt17	T	A	5: 131,110,434 (GRCm39)	M302L	probably benign	Het
Gpn3	T	C	5: 122,520,175 (GRCm39)	I243T	probably benign	Het
Gpr160	T	C	3: 30,950,141 (GRCm39)	V71A	possibly damaging	Het
Gramd1b	G	T	9: 40,244,946 (GRCm39)	A154D	probably damaging	Het
Grid2ip	G	A	5: 143,366,184 (GRCm39)	S379N	probably damaging	Het
H60b	C	A	10: 22,163,043 (GRCm39)	T206N	probably benign	Het
Hydin	A	T	8: 111,320,434 (GRCm39)	I4493F	probably benign	Het
Il19	C	T	1: 130,866,852 (GRCm39)	E43K	possibly damaging	Het
Ints2	A	G	11: 86,115,884 (GRCm39)	Y782H	probably damaging	Het
Itprid1	T	A	6: 55,851,306 (GRCm39)	I62N	probably damaging	Het
Jph1	C	A	1: 17,167,810 (GRCm39)	D7Y	probably damaging	Het
Lipo2	T	C	19: 33,726,852 (GRCm39)	I62V	probably damaging	Het
Map7	G	A	10: 20,152,026 (GRCm39)		probably null	Het
Matk	T	G	10: 81,095,422 (GRCm39)	W81G	probably damaging	Het
Matn4	T	C	2: 164,242,735 (GRCm39)	Y121C	probably damaging	Het
Mical2	G	A	7: 111,923,293 (GRCm39)		probably null	Het
Miga2	T	G	2: 30,271,674 (GRCm39)	Y399D	probably damaging	Het
Mocos	A	T	18: 24,799,672 (GRCm39)	E302V	probably benign	Het
Mrpl11	C	T	19: 5,014,743 (GRCm39)	A172V	probably damaging	Het
Mrpl48	A	C	7: 100,209,001 (GRCm39)	Y108D	probably damaging	Het
Mtr	A	G	13: 12,205,278 (GRCm39)	S1061P	probably benign	Het
Myh11	T	A	16: 14,026,155 (GRCm39)	K1309*	probably null	Het
Myom2	G	A	8: 15,154,173 (GRCm39)	V704I	possibly damaging	Het
Nars2	A	G	7: 96,706,728 (GRCm39)	H413R	probably benign	Het
Nckap1	A	G	2: 80,355,946 (GRCm39)	L619P	probably damaging	Het
Notch1	T	C	2: 26,362,817 (GRCm39)	N983S	probably damaging	Het
Nup210	A	G	6: 91,002,337 (GRCm39)	V717A	probably benign	Het
P4ha3	G	T	7: 99,966,292 (GRCm39)	G479V	probably benign	Het
Pcdhb18	G	A	18: 37,623,537 (GRCm39)	R289Q	probably benign	Het
Phf11a	A	G	14: 59,525,028 (GRCm39)	S59P	probably damaging	Het
Phyhip	G	A	14: 70,700,798 (GRCm39)	S95N	probably benign	Het
Ppat	A	G	5: 77,065,993 (GRCm39)	V375A	probably benign	Het
Prkdc	A	G	16: 15,608,456 (GRCm39)	E3086G	probably damaging	Het
Psg22	A	G	7: 18,453,599 (GRCm39)	E98G	probably damaging	Het
Rabgap1	A	T	2: 37,453,610 (GRCm39)	K1013*	probably null	Het
Retreg3	C	T	11: 101,010,526 (GRCm39)	G27D	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rptn	C	G	3: 93,305,437 (GRCm39)	H923Q	possibly damaging	Het
Sema6a	A	T	18: 47,431,369 (GRCm39)		probably null	Het
Septin10	T	C	10: 59,006,670 (GRCm39)	E349G	probably damaging	Het
Serpina1c	A	G	12: 103,863,429 (GRCm39)	V257A	probably damaging	Het
Sgpp2	T	A	1: 78,367,119 (GRCm39)	M84K	probably damaging	Het
Shoc1	A	G	4: 59,043,869 (GRCm39)	*1482R	probably null	Het
Skint11	A	G	4: 114,101,907 (GRCm39)	S116G	possibly damaging	Het
Slc2a5	G	A	4: 150,227,557 (GRCm39)	V459I	probably benign	Het
Smarca2	T	G	19: 26,748,404 (GRCm39)	Y126*	probably null	Het
Stab2	T	G	10: 86,758,867 (GRCm39)	N1024H	possibly damaging	Het
Sting1	A	T	18: 35,869,155 (GRCm39)	I178N	probably damaging	Het
Tbc1d2	G	A	4: 46,614,068 (GRCm39)	T671I	probably damaging	Het
Thoc5	T	C	11: 4,855,697 (GRCm39)	I82T	probably damaging	Het
Tmem213	G	T	6: 38,092,517 (GRCm39)	C83F	probably damaging	Het
Topaz1	G	A	9: 122,579,570 (GRCm39)	D827N	possibly damaging	Het
Trappc10	C	A	10: 78,050,646 (GRCm39)	G265V	possibly damaging	Het
Ttn	A	G	2: 76,539,808 (GRCm39)	S26066P	probably damaging	Het
Zfp292	G	T	4: 34,809,442 (GRCm39)	Q1201K	probably benign	Het
Zfp354a	T	A	11: 50,951,815 (GRCm39)		probably null	Het
Zfp418	T	C	7: 7,185,096 (GRCm39)	V353A	possibly damaging	Het
Zfp444	G	A	7: 6,192,948 (GRCm39)		probably benign	Het
Zfp503	C	A	14: 22,035,778 (GRCm39)	Q379H	probably damaging	Het
Zfp804a	A	G	2: 82,088,462 (GRCm39)	K764E	probably damaging	Het

Other mutations in Svep1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00475:Svep1	APN	4	58,176,077 (GRCm39)	missense	probably damaging	0.98
IGL00489:Svep1	APN	4	58,068,988 (GRCm39)	missense	possibly damaging	0.71
IGL00496:Svep1	APN	4	58,069,001 (GRCm39)	missense	possibly damaging	0.95
IGL00864:Svep1	APN	4	58,068,533 (GRCm39)	nonsense	probably null
IGL00904:Svep1	APN	4	58,097,398 (GRCm39)	missense	probably benign	0.00
IGL00935:Svep1	APN	4	58,090,664 (GRCm39)	missense	possibly damaging	0.71
IGL00963:Svep1	APN	4	58,072,791 (GRCm39)	nonsense	probably null
IGL01077:Svep1	APN	4	58,068,760 (GRCm39)	missense	possibly damaging	0.71
IGL01084:Svep1	APN	4	58,111,419 (GRCm39)	missense	possibly damaging	0.71
IGL01150:Svep1	APN	4	58,070,302 (GRCm39)	missense	probably benign	0.04
IGL01161:Svep1	APN	4	58,146,569 (GRCm39)	missense	probably damaging	0.96
IGL01360:Svep1	APN	4	58,116,554 (GRCm39)	missense	possibly damaging	0.73
IGL01365:Svep1	APN	4	58,100,878 (GRCm39)	critical splice acceptor site	probably null
IGL01396:Svep1	APN	4	58,068,552 (GRCm39)	missense	possibly damaging	0.85
IGL01601:Svep1	APN	4	58,084,872 (GRCm39)	missense	probably damaging	1.00
IGL01636:Svep1	APN	4	58,116,622 (GRCm39)	missense	possibly damaging	0.96
IGL01838:Svep1	APN	4	58,121,910 (GRCm39)	missense	possibly damaging	0.72
IGL01949:Svep1	APN	4	58,176,006 (GRCm39)	missense	probably damaging	1.00
IGL01984:Svep1	APN	4	58,068,877 (GRCm39)	missense	possibly damaging	0.93
IGL02005:Svep1	APN	4	58,069,056 (GRCm39)	missense	possibly damaging	0.93
IGL02036:Svep1	APN	4	58,088,245 (GRCm39)	missense	possibly damaging	0.85
IGL02039:Svep1	APN	4	58,123,980 (GRCm39)	critical splice donor site	probably null
IGL02043:Svep1	APN	4	58,068,556 (GRCm39)	missense	probably benign	0.19
IGL02073:Svep1	APN	4	58,070,104 (GRCm39)	missense	probably benign	0.06
IGL02188:Svep1	APN	4	58,068,382 (GRCm39)	missense	possibly damaging	0.71
IGL02256:Svep1	APN	4	58,070,311 (GRCm39)	missense	possibly damaging	0.71
IGL02284:Svep1	APN	4	58,072,819 (GRCm39)	missense	probably benign	0.32
IGL02323:Svep1	APN	4	58,070,236 (GRCm39)	nonsense	probably null
IGL02440:Svep1	APN	4	58,145,293 (GRCm39)	missense	probably benign	0.06
IGL02449:Svep1	APN	4	58,070,296 (GRCm39)	missense	possibly damaging	0.71
IGL02501:Svep1	APN	4	58,145,341 (GRCm39)	splice site	probably benign
IGL02568:Svep1	APN	4	58,135,441 (GRCm39)	missense	probably benign	0.42
IGL02625:Svep1	APN	4	58,115,807 (GRCm39)	missense	possibly damaging	0.53
IGL02795:Svep1	APN	4	58,123,223 (GRCm39)	missense	probably damaging	1.00
IGL02818:Svep1	APN	4	58,069,804 (GRCm39)	missense	possibly damaging	0.71
IGL02871:Svep1	APN	4	58,100,871 (GRCm39)	missense	probably benign
IGL02875:Svep1	APN	4	58,082,821 (GRCm39)	splice site	probably benign
IGL02887:Svep1	APN	4	58,145,301 (GRCm39)	missense	probably damaging	1.00
IGL03240:Svep1	APN	4	58,048,188 (GRCm39)	missense	possibly damaging	0.73
IGL03243:Svep1	APN	4	58,133,387 (GRCm39)	missense	probably benign	0.06
IGL03264:Svep1	APN	4	58,066,422 (GRCm39)	splice site	probably benign
IGL03288:Svep1	APN	4	58,116,532 (GRCm39)	missense	probably benign	0.01
IGL03340:Svep1	APN	4	58,111,451 (GRCm39)	missense	possibly damaging	0.96
IGL03341:Svep1	APN	4	58,070,308 (GRCm39)	nonsense	probably null
IGL03348:Svep1	APN	4	58,113,635 (GRCm39)	missense	probably damaging	1.00
R0001:Svep1	UTSW	4	58,066,460 (GRCm39)	missense	possibly damaging	0.93
R0042:Svep1	UTSW	4	58,123,192 (GRCm39)	missense	possibly damaging	0.92
R0042:Svep1	UTSW	4	58,123,192 (GRCm39)	missense	possibly damaging	0.92
R0125:Svep1	UTSW	4	58,099,937 (GRCm39)	splice site	probably benign
R0142:Svep1	UTSW	4	58,118,232 (GRCm39)	missense	probably benign	0.33
R0147:Svep1	UTSW	4	58,116,608 (GRCm39)	missense	possibly damaging	0.85
R0148:Svep1	UTSW	4	58,116,608 (GRCm39)	missense	possibly damaging	0.85
R0157:Svep1	UTSW	4	58,069,830 (GRCm39)	missense	possibly damaging	0.72
R0195:Svep1	UTSW	4	58,089,514 (GRCm39)	missense	possibly damaging	0.82
R0197:Svep1	UTSW	4	58,070,851 (GRCm39)	missense	possibly damaging	0.71
R0257:Svep1	UTSW	4	58,179,610 (GRCm39)	missense	possibly damaging	0.71
R0314:Svep1	UTSW	4	58,096,331 (GRCm39)	missense	possibly damaging	0.71
R0316:Svep1	UTSW	4	58,072,737 (GRCm39)	missense	probably damaging	0.98
R0322:Svep1	UTSW	4	58,057,996 (GRCm39)	splice site	probably benign
R0426:Svep1	UTSW	4	58,073,333 (GRCm39)	missense	possibly damaging	0.87
R0446:Svep1	UTSW	4	58,088,280 (GRCm39)	missense	probably damaging	1.00
R0457:Svep1	UTSW	4	58,118,136 (GRCm39)	missense	probably damaging	1.00
R0471:Svep1	UTSW	4	58,054,700 (GRCm39)	missense	possibly damaging	0.85
R0555:Svep1	UTSW	4	58,128,858 (GRCm39)	missense	possibly damaging	0.71
R0634:Svep1	UTSW	4	58,070,661 (GRCm39)	missense	possibly damaging	0.86
R0636:Svep1	UTSW	4	58,073,121 (GRCm39)	nonsense	probably null
R0827:Svep1	UTSW	4	58,053,113 (GRCm39)	splice site	probably benign
R1025:Svep1	UTSW	4	58,087,817 (GRCm39)	missense	possibly damaging	0.86
R1027:Svep1	UTSW	4	58,094,084 (GRCm39)	missense	possibly damaging	0.86
R1069:Svep1	UTSW	4	58,070,239 (GRCm39)	missense	probably damaging	1.00
R1161:Svep1	UTSW	4	58,069,416 (GRCm39)	missense	possibly damaging	0.71
R1245:Svep1	UTSW	4	58,066,427 (GRCm39)	critical splice donor site	probably null
R1282:Svep1	UTSW	4	58,100,032 (GRCm39)	missense	possibly damaging	0.93
R1310:Svep1	UTSW	4	58,069,416 (GRCm39)	missense	possibly damaging	0.71
R1444:Svep1	UTSW	4	58,115,754 (GRCm39)	missense	possibly damaging	0.53
R1460:Svep1	UTSW	4	58,068,740 (GRCm39)	missense	possibly damaging	0.85
R1500:Svep1	UTSW	4	58,070,239 (GRCm39)	missense	probably damaging	1.00
R1628:Svep1	UTSW	4	58,107,561 (GRCm39)	missense	probably benign	0.00
R1712:Svep1	UTSW	4	58,070,629 (GRCm39)	missense	probably benign	0.06
R1774:Svep1	UTSW	4	58,146,562 (GRCm39)	missense	possibly damaging	0.92
R1783:Svep1	UTSW	4	58,073,333 (GRCm39)	missense	probably benign
R1829:Svep1	UTSW	4	58,096,310 (GRCm39)	missense	possibly damaging	0.93
R1978:Svep1	UTSW	4	58,097,292 (GRCm39)	missense	possibly damaging	0.73
R1993:Svep1	UTSW	4	58,064,170 (GRCm39)	critical splice donor site	probably null
R2017:Svep1	UTSW	4	58,070,568 (GRCm39)	missense	probably benign	0.08
R2058:Svep1	UTSW	4	58,084,554 (GRCm39)	missense	possibly damaging	0.92
R2109:Svep1	UTSW	4	58,206,030 (GRCm39)	missense	possibly damaging	0.51
R2215:Svep1	UTSW	4	58,138,602 (GRCm39)	splice site	probably benign
R2281:Svep1	UTSW	4	58,082,677 (GRCm39)	missense	possibly damaging	0.85
R2504:Svep1	UTSW	4	58,135,628 (GRCm39)	splice site	probably null
R2763:Svep1	UTSW	4	58,084,061 (GRCm39)	missense	possibly damaging	0.86
R3122:Svep1	UTSW	4	58,087,845 (GRCm39)	missense	possibly damaging	0.51
R3605:Svep1	UTSW	4	58,066,542 (GRCm39)	missense	probably benign	0.32
R3763:Svep1	UTSW	4	58,084,833 (GRCm39)	missense	possibly damaging	0.89
R3827:Svep1	UTSW	4	58,096,177 (GRCm39)	missense	probably damaging	0.98
R3829:Svep1	UTSW	4	58,096,177 (GRCm39)	missense	probably damaging	0.98
R3830:Svep1	UTSW	4	58,096,177 (GRCm39)	missense	probably damaging	0.98
R3910:Svep1	UTSW	4	58,145,156 (GRCm39)	critical splice donor site	probably null
R3943:Svep1	UTSW	4	58,084,807 (GRCm39)	splice site	probably null
R3944:Svep1	UTSW	4	58,084,807 (GRCm39)	splice site	probably null
R4153:Svep1	UTSW	4	58,089,426 (GRCm39)	missense	possibly damaging	0.52
R4154:Svep1	UTSW	4	58,069,068 (GRCm39)	missense	possibly damaging	0.71
R4191:Svep1	UTSW	4	58,046,601 (GRCm39)	missense	possibly damaging	0.86
R4355:Svep1	UTSW	4	58,138,695 (GRCm39)	missense	possibly damaging	0.71
R4388:Svep1	UTSW	4	58,069,249 (GRCm39)	missense	possibly damaging	0.93
R4532:Svep1	UTSW	4	58,068,886 (GRCm39)	missense	possibly damaging	0.52
R4584:Svep1	UTSW	4	58,068,526 (GRCm39)	nonsense	probably null
R4592:Svep1	UTSW	4	58,084,028 (GRCm39)	missense	possibly damaging	0.93
R4593:Svep1	UTSW	4	58,091,944 (GRCm39)	missense	possibly damaging	0.71
R4625:Svep1	UTSW	4	58,072,698 (GRCm39)	missense	probably damaging	0.98
R4639:Svep1	UTSW	4	58,082,724 (GRCm39)	missense	probably benign
R4700:Svep1	UTSW	4	58,097,323 (GRCm39)	missense	possibly damaging	0.71
R4720:Svep1	UTSW	4	58,205,869 (GRCm39)	missense	possibly damaging	0.71
R4724:Svep1	UTSW	4	58,070,752 (GRCm39)	missense	possibly damaging	0.71
R4753:Svep1	UTSW	4	58,053,212 (GRCm39)	missense	probably benign	0.06
R4781:Svep1	UTSW	4	58,070,340 (GRCm39)	missense	probably damaging	0.98
R4820:Svep1	UTSW	4	58,082,664 (GRCm39)	missense	probably benign	0.27
R4896:Svep1	UTSW	4	58,087,751 (GRCm39)	missense	probably benign	0.08
R4905:Svep1	UTSW	4	58,069,308 (GRCm39)	missense	probably benign	0.00
R4910:Svep1	UTSW	4	58,096,276 (GRCm39)	missense	possibly damaging	0.71
R4972:Svep1	UTSW	4	58,087,778 (GRCm39)	missense	possibly damaging	0.71
R5004:Svep1	UTSW	4	58,087,751 (GRCm39)	missense	probably benign	0.08
R5088:Svep1	UTSW	4	58,120,648 (GRCm39)	missense	possibly damaging	0.73
R5112:Svep1	UTSW	4	58,068,610 (GRCm39)	nonsense	probably null
R5185:Svep1	UTSW	4	58,084,534 (GRCm39)	missense	probably damaging	0.99
R5302:Svep1	UTSW	4	58,096,183 (GRCm39)	missense	possibly damaging	0.71
R5307:Svep1	UTSW	4	58,072,677 (GRCm39)	missense	possibly damaging	0.71
R5339:Svep1	UTSW	4	58,121,892 (GRCm39)	missense	possibly damaging	0.96
R5379:Svep1	UTSW	4	58,072,991 (GRCm39)	missense	possibly damaging	0.51
R5384:Svep1	UTSW	4	58,104,545 (GRCm39)	missense	possibly damaging	0.71
R5414:Svep1	UTSW	4	58,206,322 (GRCm39)	missense	possibly damaging	0.53
R5514:Svep1	UTSW	4	58,044,054 (GRCm39)	missense	possibly damaging	0.53
R5538:Svep1	UTSW	4	58,049,282 (GRCm39)	critical splice acceptor site	probably null
R5549:Svep1	UTSW	4	58,057,954 (GRCm39)	missense	probably benign	0.32
R5618:Svep1	UTSW	4	58,070,537 (GRCm39)	missense	probably benign
R5623:Svep1	UTSW	4	58,091,964 (GRCm39)	missense	possibly damaging	0.92
R5686:Svep1	UTSW	4	58,072,826 (GRCm39)	missense	possibly damaging	0.71
R5743:Svep1	UTSW	4	58,096,223 (GRCm39)	missense	possibly damaging	0.71
R5773:Svep1	UTSW	4	58,099,985 (GRCm39)	missense	possibly damaging	0.86
R5809:Svep1	UTSW	4	58,116,524 (GRCm39)	missense	possibly damaging	0.73
R5896:Svep1	UTSW	4	58,084,906 (GRCm39)	missense	possibly damaging	0.71
R5918:Svep1	UTSW	4	58,069,345 (GRCm39)	missense	possibly damaging	0.71
R5969:Svep1	UTSW	4	58,070,977 (GRCm39)	nonsense	probably null
R6010:Svep1	UTSW	4	58,115,832 (GRCm39)	missense	possibly damaging	0.95
R6187:Svep1	UTSW	4	58,072,872 (GRCm39)	missense	probably damaging	1.00
R6192:Svep1	UTSW	4	58,104,536 (GRCm39)	missense	possibly damaging	0.92
R6234:Svep1	UTSW	4	58,113,458 (GRCm39)	splice site	probably null
R6326:Svep1	UTSW	4	58,073,045 (GRCm39)	missense	possibly damaging	0.51
R6400:Svep1	UTSW	4	58,049,169 (GRCm39)	missense	probably damaging	1.00
R6418:Svep1	UTSW	4	58,053,126 (GRCm39)	missense	probably benign	0.01
R6440:Svep1	UTSW	4	58,116,555 (GRCm39)	missense	possibly damaging	0.53
R6489:Svep1	UTSW	4	58,100,066 (GRCm39)	missense	probably damaging	1.00
R6515:Svep1	UTSW	4	58,088,280 (GRCm39)	missense	probably damaging	1.00
R6738:Svep1	UTSW	4	58,123,180 (GRCm39)	missense	possibly damaging	0.71
R6773:Svep1	UTSW	4	58,049,146 (GRCm39)	missense	possibly damaging	0.71
R6796:Svep1	UTSW	4	58,064,275 (GRCm39)	missense	probably benign	0.01
R7055:Svep1	UTSW	4	58,120,642 (GRCm39)	missense	probably benign	0.33
R7055:Svep1	UTSW	4	58,064,275 (GRCm39)	missense	probably benign	0.19
R7111:Svep1	UTSW	4	58,118,207 (GRCm39)	missense	possibly damaging	0.70
R7161:Svep1	UTSW	4	58,128,859 (GRCm39)	missense	possibly damaging	0.93
R7162:Svep1	UTSW	4	58,070,262 (GRCm39)	missense	possibly damaging	0.71
R7182:Svep1	UTSW	4	58,043,991 (GRCm39)	missense	probably benign	0.18
R7292:Svep1	UTSW	4	58,111,395 (GRCm39)	missense	possibly damaging	0.71
R7299:Svep1	UTSW	4	58,046,587 (GRCm39)	nonsense	probably null
R7301:Svep1	UTSW	4	58,046,587 (GRCm39)	nonsense	probably null
R7316:Svep1	UTSW	4	58,068,763 (GRCm39)	missense	possibly damaging	0.71
R7337:Svep1	UTSW	4	58,108,323 (GRCm39)	missense	probably damaging	0.98
R7391:Svep1	UTSW	4	58,145,185 (GRCm39)	missense	probably damaging	0.98
R7402:Svep1	UTSW	4	58,069,699 (GRCm39)	missense	possibly damaging	0.71
R7445:Svep1	UTSW	4	58,094,122 (GRCm39)	missense	possibly damaging	0.85
R7450:Svep1	UTSW	4	58,064,248 (GRCm39)	missense	possibly damaging	0.71
R7492:Svep1	UTSW	4	58,066,468 (GRCm39)	missense	possibly damaging	0.51
R7505:Svep1	UTSW	4	58,115,862 (GRCm39)	missense	possibly damaging	0.53
R7509:Svep1	UTSW	4	58,090,683 (GRCm39)	missense	probably benign	0.40
R7538:Svep1	UTSW	4	58,053,260 (GRCm39)	missense	possibly damaging	0.71
R7555:Svep1	UTSW	4	58,069,422 (GRCm39)	missense	probably damaging	0.98
R7660:Svep1	UTSW	4	58,087,782 (GRCm39)	missense	probably benign	0.32
R7670:Svep1	UTSW	4	58,097,424 (GRCm39)	missense	probably damaging	1.00
R7719:Svep1	UTSW	4	58,068,523 (GRCm39)	missense	probably damaging	0.97
R7733:Svep1	UTSW	4	58,049,239 (GRCm39)	missense	probably benign	0.03
R7781:Svep1	UTSW	4	58,069,251 (GRCm39)	missense	possibly damaging	0.71
R7821:Svep1	UTSW	4	58,179,601 (GRCm39)	missense	probably damaging	0.99
R7832:Svep1	UTSW	4	58,054,539 (GRCm39)	missense	probably benign	0.44
R8017:Svep1	UTSW	4	58,146,637 (GRCm39)	missense	probably damaging	0.99
R8019:Svep1	UTSW	4	58,146,637 (GRCm39)	missense	probably damaging	0.99
R8066:Svep1	UTSW	4	58,113,650 (GRCm39)	missense	probably benign	0.33
R8159:Svep1	UTSW	4	58,087,815 (GRCm39)	missense	probably benign	0.01
R8159:Svep1	UTSW	4	58,069,396 (GRCm39)	missense	possibly damaging	0.71
R8170:Svep1	UTSW	4	58,069,378 (GRCm39)	missense	probably benign	0.00
R8246:Svep1	UTSW	4	58,091,889 (GRCm39)	missense	probably damaging	0.96
R8392:Svep1	UTSW	4	58,070,566 (GRCm39)	missense	possibly damaging	0.71
R8436:Svep1	UTSW	4	58,044,053 (GRCm39)	missense	possibly damaging	0.86
R8544:Svep1	UTSW	4	58,206,025 (GRCm39)	missense	probably benign	0.00
R8669:Svep1	UTSW	4	58,070,119 (GRCm39)	missense	possibly damaging	0.95
R8707:Svep1	UTSW	4	58,070,197 (GRCm39)	nonsense	probably null
R8790:Svep1	UTSW	4	58,118,145 (GRCm39)	missense	possibly damaging	0.53
R8804:Svep1	UTSW	4	58,206,043 (GRCm39)	missense	possibly damaging	0.86
R8868:Svep1	UTSW	4	58,135,578 (GRCm39)	missense	possibly damaging	0.77
R8880:Svep1	UTSW	4	58,064,204 (GRCm39)	missense	possibly damaging	0.51
R8949:Svep1	UTSW	4	58,054,604 (GRCm39)	missense	possibly damaging	0.85
R9007:Svep1	UTSW	4	58,091,915 (GRCm39)	missense	possibly damaging	0.86
R9028:Svep1	UTSW	4	58,145,199 (GRCm39)	missense	possibly damaging	0.92
R9131:Svep1	UTSW	4	58,087,778 (GRCm39)	missense	possibly damaging	0.71
R9285:Svep1	UTSW	4	58,084,809 (GRCm39)	critical splice donor site	probably null
R9302:Svep1	UTSW	4	58,120,565 (GRCm39)	missense	possibly damaging	0.53
R9314:Svep1	UTSW	4	58,070,347 (GRCm39)	missense	probably damaging	1.00
R9427:Svep1	UTSW	4	58,069,804 (GRCm39)	missense	possibly damaging	0.71
R9443:Svep1	UTSW	4	58,179,697 (GRCm39)	missense	possibly damaging	0.95
R9473:Svep1	UTSW	4	58,064,243 (GRCm39)	missense	probably benign	0.00
R9487:Svep1	UTSW	4	58,070,517 (GRCm39)	missense	probably benign
R9494:Svep1	UTSW	4	58,070,577 (GRCm39)	missense	possibly damaging	0.51
R9515:Svep1	UTSW	4	58,084,144 (GRCm39)	missense	possibly damaging	0.71
R9681:Svep1	UTSW	4	58,084,959 (GRCm39)	missense	probably damaging	0.98
X0063:Svep1	UTSW	4	58,070,468 (GRCm39)	nonsense	probably null
Z1176:Svep1	UTSW	4	58,133,415 (GRCm39)	missense	possibly damaging	0.51
Z1176:Svep1	UTSW	4	58,115,814 (GRCm39)	missense	possibly damaging	0.93
Z1176:Svep1	UTSW	4	58,111,386 (GRCm39)	missense	probably damaging	0.97
Z1177:Svep1	UTSW	4	58,206,300 (GRCm39)	missense	probably damaging	0.99
Z1177:Svep1	UTSW	4	58,115,841 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- GGCAGCAGACACTGACGAATAC -3'
(R):5'- GTTCTGAAGATCACACAAGAGTATG -3'

Sequencing Primer
(F):5'- CACTGACGAATACAAAGGATTGTC -3'
(R):5'- GATCACACAAGAGTATGAAAGTGTTC -3'

Posted On

2018-02-27