Mutagenetix > Incidental Mutation

Incidental Mutation 'R6216:Vmn2r101'

503748

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r101

Ensembl Gene

ENSMUSG00000094892

Gene Name

vomeronasal 2, receptor 101

Synonyms

EG627576

MMRRC Submission

044349-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R6216 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19577231-19612317 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19591005 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 450 (S450R)

Ref Sequence

ENSEMBL: ENSMUSP00000131583 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171914]

AlphaFold

E9PZS9

Predicted Effect

probably benign
Transcript: ENSMUST00000171914
AA Change: S450R

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000131583
Gene: ENSMUSG00000094892
AA Change: S450R

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	82	466	1.6e-36	PFAM
Pfam:NCD3G	509	562	6.4e-22	PFAM
Pfam:7tm_3	595	830	1.4e-51	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

97% (67/69)

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	T	C	9: 57,257,627	D488G	probably benign	Het
Adgrv1	C	A	13: 81,524,471		probably null	Het
Afg3l2	G	T	18: 67,421,259	L458M	probably damaging	Het
Alkbh3	G	A	2: 94,008,536		probably benign	Het
Ankrd22	A	T	19: 34,124,169		probably null	Het
Anks1b	A	G	10: 90,260,756	D425G	probably damaging	Het
Arid2	A	G	15: 96,356,909	D212G	probably benign	Het
Bbx	A	T	16: 50,251,388	S225T	probably benign	Het
Bivm	T	A	1: 44,126,868		probably null	Het
Bpifb3	A	G	2: 153,925,853	Y282C	probably benign	Het
Cacna1h	A	T	17: 25,378,819	I1767N	probably damaging	Het
Ccdc28a	G	T	10: 18,224,971	S36*	probably null	Het
Ccser2	A	G	14: 36,940,508	S240P	probably damaging	Het
Ceacam1	A	T	7: 25,471,996	S348T	probably benign	Het
Ddx49	C	T	8: 70,297,284	G161D	probably damaging	Het
Dhx16	A	G	17: 35,882,972	E353G	possibly damaging	Het
Etv2	A	G	7: 30,634,611		probably null	Het
Extl1	T	C	4: 134,363,130	T345A	probably benign	Het
Fam163a	A	T	1: 156,078,995	S137T	probably benign	Het
Fermt2	A	T	14: 45,459,881	M671K	possibly damaging	Het
Fsd1l	T	C	4: 53,694,742	C388R	probably damaging	Het
Galnt18	A	C	7: 111,513,550	V470G	probably benign	Het
Grin2b	T	A	6: 135,772,399	I602F	probably damaging	Het
Gucy1b1	G	T	3: 82,046,713		probably null	Het
Hace1	T	C	10: 45,618,547	V151A	probably benign	Het
Hadhb	T	A	5: 30,174,931	D258E	probably benign	Het
Heatr1	C	T	13: 12,432,664	T1746I	probably benign	Het
Hrnr	T	C	3: 93,332,162	S3236P	unknown	Het
Kcnd1	G	C	X: 7,823,909	A23P	probably damaging	Homo
Kiz	C	A	2: 146,889,497	D302E	probably damaging	Het
Kng2	A	T	16: 22,987,593	W619R	probably damaging	Het
Mfap3l	C	A	8: 60,671,807	T361K	probably damaging	Het
Mlec	T	C	5: 115,150,317	H160R	probably benign	Het
Myo16	T	C	8: 10,315,494	L89P	probably benign	Het
Neb	T	C	2: 52,224,552	I4232V	probably benign	Het
Nf1	A	G	11: 79,411,607	I334V	possibly damaging	Het
Nipbl	A	C	15: 8,318,383	F1942V	probably damaging	Het
Olfr1205	T	C	2: 88,831,311	S65P	probably damaging	Het
Olfr1242	C	A	2: 89,493,722	V197F	probably damaging	Het
Olfr642	A	G	7: 104,049,695	Y220H	probably damaging	Het
Olfr699	A	T	7: 106,790,458	I181N	probably benign	Het
Pabpc2	G	T	18: 39,774,719	A346S	probably damaging	Het
Paxip1	T	C	5: 27,766,173	T393A	unknown	Het
Pcdhgb5	A	T	18: 37,731,928	T259S	probably benign	Het
Pfkp	A	T	13: 6,619,188	L253H	probably benign	Het
Piezo1	T	C	8: 122,489,130	D1428G	probably benign	Het
Pih1d3	A	T	1: 31,223,351	D138V	probably damaging	Het
Pkd1l2	T	G	8: 117,081,470	D105A	probably damaging	Het
Pou2f1	G	T	1: 165,880,320		probably benign	Het
Ppfia4	T	C	1: 134,329,183	E100G	probably damaging	Het
Ppp1r15a	G	T	7: 45,524,022	T454K	probably damaging	Het
Prpsap1	A	T	11: 116,471,413	I381N	probably damaging	Het
Ptx3	T	A	3: 66,224,844	V262E	probably damaging	Het
Rab21	A	T	10: 115,294,926	H158Q	probably benign	Het
Rasa2	A	G	9: 96,544,304	S830P	probably damaging	Het
Rdh1	G	A	10: 127,764,753	S215N	probably benign	Het
Rhot1	G	A	11: 80,251,059	R463H	probably benign	Het
Rsf1	CG	CGACGGCGGGG	7: 97,579,908		probably benign	Het
Sart3	C	T	5: 113,743,206	A938T	probably benign	Het
Selenom	A	T	11: 3,514,915		probably benign	Het
Selenop	T	C	15: 3,279,465	C300R	probably damaging	Het
Skint1	T	A	4: 112,021,482	S204T	probably benign	Het
Ss18l1	A	G	2: 180,061,913	N313S	unknown	Het
Sycp2l	A	G	13: 41,141,724	D289G	probably damaging	Het
Terf1	C	T	1: 15,818,997	H188Y	probably benign	Het
Tmem116	C	T	5: 121,491,108	T188M	probably benign	Het
Trdn	A	G	10: 33,305,069	T357A	probably damaging	Het
Trip11	A	C	12: 101,890,600	M401R	probably benign	Het
Ubald2	A	C	11: 116,434,385	D26A	probably benign	Het
Ube2w	C	G	1: 16,619,284		probably benign	Het

Other mutations in Vmn2r101

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01121:Vmn2r101	APN	17	19589674	missense	probably damaging	0.99
IGL02125:Vmn2r101	APN	17	19589701	missense	possibly damaging	0.95
IGL02300:Vmn2r101	APN	17	19611937	missense	probably damaging	1.00
IGL02682:Vmn2r101	APN	17	19612245	missense	possibly damaging	0.82
IGL02825:Vmn2r101	APN	17	19589870	missense	probably benign	0.00
IGL02862:Vmn2r101	APN	17	19611605	missense	probably damaging	1.00
IGL02943:Vmn2r101	APN	17	19611404	missense	probably damaging	0.99
R0371:Vmn2r101	UTSW	17	19590132	missense	probably benign	0.07
R0462:Vmn2r101	UTSW	17	19590169	missense	probably benign	0.04
R0492:Vmn2r101	UTSW	17	19588983	missense	probably damaging	1.00
R0654:Vmn2r101	UTSW	17	19590111	missense	probably benign	0.01
R1120:Vmn2r101	UTSW	17	19577461	splice site	probably benign
R1323:Vmn2r101	UTSW	17	19612051	missense	probably damaging	1.00
R1323:Vmn2r101	UTSW	17	19612051	missense	probably damaging	1.00
R1676:Vmn2r101	UTSW	17	19611922	missense	probably benign	0.00
R2023:Vmn2r101	UTSW	17	19590106	nonsense	probably null
R2149:Vmn2r101	UTSW	17	19588963	missense	probably benign	0.00
R2350:Vmn2r101	UTSW	17	19589783	missense	probably benign	0.01
R2760:Vmn2r101	UTSW	17	19589639	missense	probably benign	0.14
R3085:Vmn2r101	UTSW	17	19588815	splice site	probably null
R3086:Vmn2r101	UTSW	17	19588815	splice site	probably null
R3719:Vmn2r101	UTSW	17	19589549	missense	possibly damaging	0.50
R3771:Vmn2r101	UTSW	17	19589657	missense	probably benign
R3773:Vmn2r101	UTSW	17	19589657	missense	probably benign
R4225:Vmn2r101	UTSW	17	19611689	missense	probably damaging	1.00
R4248:Vmn2r101	UTSW	17	19589114	missense	probably damaging	1.00
R4290:Vmn2r101	UTSW	17	19612041	missense	probably damaging	1.00
R4291:Vmn2r101	UTSW	17	19612041	missense	probably damaging	1.00
R4293:Vmn2r101	UTSW	17	19612041	missense	probably damaging	1.00
R4307:Vmn2r101	UTSW	17	19590161	missense	probably damaging	1.00
R4721:Vmn2r101	UTSW	17	19612025	missense	probably damaging	0.99
R4829:Vmn2r101	UTSW	17	19611967	missense	probably benign	0.03
R5022:Vmn2r101	UTSW	17	19611387	critical splice acceptor site	probably null
R5110:Vmn2r101	UTSW	17	19611635	missense	possibly damaging	0.92
R5244:Vmn2r101	UTSW	17	19611526	missense	probably damaging	1.00
R5397:Vmn2r101	UTSW	17	19588842	missense	probably damaging	1.00
R5875:Vmn2r101	UTSW	17	19588830	missense	probably damaging	0.99
R5944:Vmn2r101	UTSW	17	19589507	missense	probably benign	0.00
R6334:Vmn2r101	UTSW	17	19589850	missense	possibly damaging	0.83
R6512:Vmn2r101	UTSW	17	19588884	missense	probably damaging	1.00
R6607:Vmn2r101	UTSW	17	19612034	missense	probably damaging	1.00
R6965:Vmn2r101	UTSW	17	19591022	missense	probably benign	0.00
R7101:Vmn2r101	UTSW	17	19589088	missense	probably null	0.14
R7183:Vmn2r101	UTSW	17	19612178	missense	probably damaging	1.00
R7344:Vmn2r101	UTSW	17	19611797	missense	probably benign	0.38
R7375:Vmn2r101	UTSW	17	19611390	missense	probably damaging	1.00
R7574:Vmn2r101	UTSW	17	19611637	missense	possibly damaging	0.91
R7575:Vmn2r101	UTSW	17	19611392	missense	probably benign	0.01
R7592:Vmn2r101	UTSW	17	19591181	splice site	probably null
R7626:Vmn2r101	UTSW	17	19611930	nonsense	probably null
R7715:Vmn2r101	UTSW	17	19611915	missense	probably damaging	1.00
R7730:Vmn2r101	UTSW	17	19611688	missense	possibly damaging	0.81
R8078:Vmn2r101	UTSW	17	19590245	missense	probably benign	0.07
R8228:Vmn2r101	UTSW	17	19591022	missense	probably benign	0.00
R8283:Vmn2r101	UTSW	17	19611991	missense	probably damaging	1.00
R8712:Vmn2r101	UTSW	17	19591135	missense	probably benign	0.24
R8765:Vmn2r101	UTSW	17	19588983	missense	probably damaging	1.00
R9091:Vmn2r101	UTSW	17	19589982	missense	probably benign
R9092:Vmn2r101	UTSW	17	19589545	missense	probably benign	0.07
R9113:Vmn2r101	UTSW	17	19591026	missense	possibly damaging	0.56
R9158:Vmn2r101	UTSW	17	19588899	missense	probably benign	0.00
R9168:Vmn2r101	UTSW	17	19588876	missense	probably damaging	1.00
R9235:Vmn2r101	UTSW	17	19589976	missense	probably damaging	0.99
R9270:Vmn2r101	UTSW	17	19589982	missense	probably benign
R9290:Vmn2r101	UTSW	17	19591133	missense	probably benign	0.05
R9296:Vmn2r101	UTSW	17	19589785	missense	probably damaging	0.98
R9602:Vmn2r101	UTSW	17	19611518	nonsense	probably null
R9706:Vmn2r101	UTSW	17	19589663	missense	probably damaging	1.00
Z1088:Vmn2r101	UTSW	17	19588975	missense	possibly damaging	0.78

Predicted Primers

PCR Primer
(F):5'- TAAGCTTGGCTCTCAGTTTTCACTG -3'
(R):5'- CCACCTCTGAAAATGTTTCTGGC -3'

Sequencing Primer
(F):5'- TCGAACACTTAGGTTTTTACTTAGTC -3'
(R):5'- ACCTCTGAAAATGTTTCTGGCCATTG -3'

Posted On

2018-02-27