Incidental Mutation 'R6235:Usp37'
ID 504843
Institutional Source Beutler Lab
Gene Symbol Usp37
Ensembl Gene ENSMUSG00000033364
Gene Name ubiquitin specific peptidase 37
Synonyms C330008N13Rik, 4932415L06Rik
MMRRC Submission
Accession Numbers
Is this an essential gene? Essential (E-score: 1.000) question?
Stock # R6235 (G1)
Quality Score 225.009
Status Validated
Chromosome 1
Chromosomal Location 74435511-74544284 bp(-) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) G to T at 74475133 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Serine to Stop codon at position 293 (S293*)
Ref Sequence ENSEMBL: ENSMUSP00000140670 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000044260] [ENSMUST00000189257]
AlphaFold no structure available at present
Predicted Effect probably null
Transcript: ENSMUST00000044260
AA Change: S293*
SMART Domains Protein: ENSMUSP00000035445
Gene: ENSMUSG00000033364
AA Change: S293*

DomainStartEndE-ValueType
Pfam:UCH_N 1 105 5.1e-47 PFAM
low complexity region 182 200 N/A INTRINSIC
Pfam:UCH_1 341 645 3.4e-16 PFAM
UIM 704 723 1.33e1 SMART
UIM 806 825 1.04e-1 SMART
UIM 828 847 2.11e-2 SMART
low complexity region 893 909 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000189257
AA Change: S293*
SMART Domains Protein: ENSMUSP00000140670
Gene: ENSMUSG00000033364
AA Change: S293*

DomainStartEndE-ValueType
PDB:3U12|B 4 125 2e-71 PDB
low complexity region 182 200 N/A INTRINSIC
Pfam:UCH_1 341 608 4.3e-19 PFAM
low complexity region 628 646 N/A INTRINSIC
UIM 704 723 1.33e1 SMART
UIM 806 825 1.04e-1 SMART
UIM 828 847 2.11e-2 SMART
low complexity region 893 909 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000191058
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.4%
  • 20x: 95.4%
Validation Efficiency 95% (73/77)
MGI Phenotype PHENOTYPE: Mice homozygous for an ENU-induced mutation exhibit complete embryonic lethality. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 77 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam6b T C 12: 113,491,710 F716L probably benign Het
Akip1 A G 7: 109,707,413 M106V probably benign Het
Aldh1l1 A T 6: 90,564,457 I278F probably benign Het
Aplnr A G 2: 85,137,626 T332A probably benign Het
Aqp4 A G 18: 15,398,113 V197A probably damaging Het
Arid4a A T 12: 71,069,772 probably null Het
Baiap2 A T 11: 119,981,408 N99Y probably damaging Het
Ceacam1 A T 7: 25,471,792 probably null Het
Cep78 T C 19: 15,976,486 probably null Het
Cldn3 T C 5: 134,986,719 F92S possibly damaging Het
Clec16a C A 16: 10,694,635 P812Q probably damaging Het
Cnga4 T G 7: 105,407,699 Y336* probably null Het
Copg2 A G 6: 30,816,071 I443T probably damaging Het
Crhbp C A 13: 95,443,850 A81S probably damaging Het
Csad A T 15: 102,178,606 V410D probably damaging Het
Ctps G A 4: 120,558,806 L207F probably benign Het
Dab2ip A G 2: 35,723,087 E1003G probably damaging Het
Ddx31 C G 2: 28,844,842 A5G probably benign Het
Dnah12 A T 14: 26,854,804 I3004F probably damaging Het
Fbxo15 A G 18: 84,980,904 probably benign Het
Fbxw28 C A 9: 109,326,190 W356C probably damaging Het
Gimap6 G T 6: 48,702,457 T215K probably benign Het
Gm5346 T G 8: 43,625,912 N425T probably benign Het
Hspa4 C T 11: 53,262,939 E702K probably benign Het
Hunk A G 16: 90,432,706 I152V probably damaging Het
Itk T C 11: 46,336,428 E456G probably benign Het
Krt40 G A 11: 99,543,094 A22V possibly damaging Het
Lars2 T C 9: 123,411,880 V204A probably damaging Het
Lipo1 A G 19: 33,783,563 Y140H probably damaging Het
Lrp1 G A 10: 127,588,177 R809W probably damaging Het
Magi3 C T 3: 104,016,068 G1111D probably damaging Het
Mis18bp1 A T 12: 65,158,408 V47E probably damaging Het
Mpdz T C 4: 81,385,281 E140G probably damaging Het
Myo1d T C 11: 80,692,944 I81V probably benign Het
Nek10 T A 14: 14,821,113 Y26* probably null Het
Ntng2 C A 2: 29,227,979 E152D probably damaging Het
Olfr1036 T C 2: 86,075,166 L142P possibly damaging Het
Olfr1490 C A 19: 13,654,781 C117* probably null Het
Otud3 A T 4: 138,901,901 V185D probably damaging Het
Parp6 C T 9: 59,630,815 R248W probably benign Het
Pcdhga7 A G 18: 37,716,430 T497A probably benign Het
Ppp1r18 A G 17: 35,873,877 E140G probably damaging Het
Prl3b1 T C 13: 27,247,945 L151P probably damaging Het
Pth1r C T 9: 110,722,316 E572K possibly damaging Het
Ptprq T A 10: 107,635,338 T1401S possibly damaging Het
Rad51ap2 A G 12: 11,457,516 T480A possibly damaging Het
Rax T A 18: 65,935,161 Q291L unknown Het
Reck T C 4: 43,937,450 L734P probably damaging Het
Rgmb A G 17: 15,820,819 F169L probably damaging Het
Rhobtb3 T C 13: 75,892,910 I426M probably damaging Het
Ring1 C A 17: 34,023,306 A76S probably damaging Het
Robo3 T G 9: 37,420,929 Y891S probably damaging Het
Rpusd2 A G 2: 119,034,857 I12V probably benign Het
Rragd T C 4: 32,995,985 V165A possibly damaging Het
Rsf1 G GACGGCGGCT 7: 97,579,909 probably benign Homo
Ryr1 G T 7: 29,116,181 Q95K probably benign Het
S1pr4 T A 10: 81,498,882 N253Y possibly damaging Het
Scn3a A C 2: 65,461,335 V1689G probably damaging Het
Sdhb A G 4: 140,973,673 N147D probably damaging Het
Sdk1 A T 5: 142,034,426 H913L possibly damaging Het
Serpina3i T A 12: 104,266,532 M232K probably damaging Het
Sipa1l2 A T 8: 125,474,871 V646E probably damaging Het
Slc38a1 A T 15: 96,578,792 I396N probably benign Het
Slc6a13 A T 6: 121,302,794 E42D probably benign Het
Stard9 G A 2: 120,713,546 V4442M probably damaging Het
Sumo3 T A 10: 77,616,237 probably benign Het
Syngap1 A G 17: 26,958,130 I356V probably benign Het
Tcam1 C T 11: 106,284,054 Q112* probably null Het
Tdp2 T A 13: 24,840,395 L225* probably null Het
Tmprss7 A G 16: 45,658,122 V747A probably benign Het
Ugt2b34 T A 5: 86,906,364 Y186F probably benign Het
Vmn1r36 A G 6: 66,716,246 I109T probably benign Het
Vsig10l T A 7: 43,468,972 V798E probably benign Het
Zfp36l1 G T 12: 80,112,822 C18* probably null Het
Zfp959 T C 17: 55,897,427 Y152H probably damaging Het
Zfyve26 A T 12: 79,249,599 C1949S probably damaging Het
Zswim9 T A 7: 13,261,603 E209V probably damaging Het
Other mutations in Usp37
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00929:Usp37 APN 1 74490154 missense probably benign 0.05
IGL00961:Usp37 APN 1 74490155 missense probably benign
IGL01089:Usp37 APN 1 74493046 nonsense probably null
IGL01348:Usp37 APN 1 74461702 missense probably damaging 0.98
IGL01609:Usp37 APN 1 74475040 missense probably benign 0.02
PIT4544001:Usp37 UTSW 1 74470579 missense possibly damaging 0.65
R0331:Usp37 UTSW 1 74454064 nonsense probably null
R0332:Usp37 UTSW 1 74495710 missense possibly damaging 0.47
R0418:Usp37 UTSW 1 74490107 missense probably benign 0.01
R0456:Usp37 UTSW 1 74468348 missense probably damaging 1.00
R1605:Usp37 UTSW 1 74493004 missense possibly damaging 0.59
R1756:Usp37 UTSW 1 74479655 missense probably benign 0.20
R1971:Usp37 UTSW 1 74439968 nonsense probably null
R2061:Usp37 UTSW 1 74468272 missense probably damaging 1.00
R2130:Usp37 UTSW 1 74461656 missense probably damaging 1.00
R2215:Usp37 UTSW 1 74444526 missense probably damaging 1.00
R2867:Usp37 UTSW 1 74450532 missense probably damaging 1.00
R2867:Usp37 UTSW 1 74450532 missense probably damaging 1.00
R3716:Usp37 UTSW 1 74492986 missense possibly damaging 0.93
R5077:Usp37 UTSW 1 74441561 missense probably damaging 0.99
R5635:Usp37 UTSW 1 74495811 start gained probably benign
R5826:Usp37 UTSW 1 74470626 missense probably damaging 0.99
R5933:Usp37 UTSW 1 74485982 missense probably damaging 0.98
R6048:Usp37 UTSW 1 74478136 splice site probably null
R6169:Usp37 UTSW 1 74495751 missense probably damaging 0.99
R6193:Usp37 UTSW 1 74492928 missense probably damaging 1.00
R6361:Usp37 UTSW 1 74453893 missense probably benign 0.06
R6572:Usp37 UTSW 1 74495782 missense possibly damaging 0.95
R6759:Usp37 UTSW 1 74495749 nonsense probably null
R6997:Usp37 UTSW 1 74453959 missense probably benign 0.01
R7471:Usp37 UTSW 1 74495628 critical splice donor site probably null
R7632:Usp37 UTSW 1 74468374 missense probably benign 0.04
R7691:Usp37 UTSW 1 74486760 frame shift probably null
R8954:Usp37 UTSW 1 74474984 critical splice donor site probably null
R9280:Usp37 UTSW 1 74450540 missense probably damaging 0.98
R9484:Usp37 UTSW 1 74459922 missense probably damaging 1.00
RF017:Usp37 UTSW 1 74470690 missense probably damaging 1.00
X0058:Usp37 UTSW 1 74453923 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AACTGGAGACTAGGTCATGGAC -3'
(R):5'- AGGCTCCTGTATGAACTTACTAAG -3'

Sequencing Primer
(F):5'- CTGGAGACTAGGTCATGGACTTAATG -3'
(R):5'- GGATCTCTCTGACTCTGCTA -3'
Posted On 2018-02-28