Incidental Mutation 'R6235:Nek10'
| ID |
504902 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nek10
|
| Ensembl Gene |
ENSMUSG00000042567 |
| Gene Name |
NIMA (never in mitosis gene a)- related kinase 10 |
| Synonyms |
LOC238944 |
| MMRRC Submission |
044433-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R6235 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
14 |
| Chromosomal Location |
7457704-7666183 bp(-) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
T to A
at 14821113 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Stop codon
at position 26
(Y26*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000153142
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000112630]
[ENSMUST00000112631]
[ENSMUST00000224491]
|
| AlphaFold |
Q3UGM2 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000112630
AA Change: Y26*
|
| SMART Domains |
Protein: ENSMUSP00000108249
Gene: ENSMUSG00000042567
AA Change: Y26*
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000112631
AA Change: Y26*
|
| SMART Domains |
Protein: ENSMUSP00000108250
Gene: ENSMUSG00000042567
AA Change: Y26*
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000224491
AA Change: Y26*
|
| Meta Mutation Damage Score |
0.9755 |
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.7%
- 10x: 98.4%
- 20x: 95.4%
|
| Validation Efficiency |
95% (73/77) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 77 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam34l |
T |
G |
8: 44,078,949 (GRCm39) |
N425T |
probably benign |
Het |
| Adam6b |
T |
C |
12: 113,455,330 (GRCm39) |
F716L |
probably benign |
Het |
| Akip1 |
A |
G |
7: 109,306,620 (GRCm39) |
M106V |
probably benign |
Het |
| Aldh1l1 |
A |
T |
6: 90,541,439 (GRCm39) |
I278F |
probably benign |
Het |
| Aplnr |
A |
G |
2: 84,967,970 (GRCm39) |
T332A |
probably benign |
Het |
| Aqp4 |
A |
G |
18: 15,531,170 (GRCm39) |
V197A |
probably damaging |
Het |
| Arid4a |
A |
T |
12: 71,116,546 (GRCm39) |
|
probably null |
Het |
| Baiap2 |
A |
T |
11: 119,872,234 (GRCm39) |
N99Y |
probably damaging |
Het |
| Ceacam1 |
A |
T |
7: 25,171,217 (GRCm39) |
|
probably null |
Het |
| Cep78 |
T |
C |
19: 15,953,850 (GRCm39) |
|
probably null |
Het |
| Cldn3 |
T |
C |
5: 135,015,573 (GRCm39) |
F92S |
possibly damaging |
Het |
| Clec16a |
C |
A |
16: 10,512,499 (GRCm39) |
P812Q |
probably damaging |
Het |
| Cnga4 |
T |
G |
7: 105,056,906 (GRCm39) |
Y336* |
probably null |
Het |
| Copg2 |
A |
G |
6: 30,793,006 (GRCm39) |
I443T |
probably damaging |
Het |
| Crhbp |
C |
A |
13: 95,580,358 (GRCm39) |
A81S |
probably damaging |
Het |
| Csad |
A |
T |
15: 102,087,041 (GRCm39) |
V410D |
probably damaging |
Het |
| Ctps1 |
G |
A |
4: 120,416,003 (GRCm39) |
L207F |
probably benign |
Het |
| Dab2ip |
A |
G |
2: 35,613,099 (GRCm39) |
E1003G |
probably damaging |
Het |
| Ddx31 |
C |
G |
2: 28,734,854 (GRCm39) |
A5G |
probably benign |
Het |
| Dnah12 |
A |
T |
14: 26,576,761 (GRCm39) |
I3004F |
probably damaging |
Het |
| Fbxo15 |
A |
G |
18: 84,999,029 (GRCm39) |
|
probably benign |
Het |
| Fbxw28 |
C |
A |
9: 109,155,258 (GRCm39) |
W356C |
probably damaging |
Het |
| Gimap6 |
G |
T |
6: 48,679,391 (GRCm39) |
T215K |
probably benign |
Het |
| Hspa4 |
C |
T |
11: 53,153,766 (GRCm39) |
E702K |
probably benign |
Het |
| Hunk |
A |
G |
16: 90,229,594 (GRCm39) |
I152V |
probably damaging |
Het |
| Itk |
T |
C |
11: 46,227,255 (GRCm39) |
E456G |
probably benign |
Het |
| Krt40 |
G |
A |
11: 99,433,920 (GRCm39) |
A22V |
possibly damaging |
Het |
| Lars2 |
T |
C |
9: 123,240,945 (GRCm39) |
V204A |
probably damaging |
Het |
| Lipo3 |
A |
G |
19: 33,760,963 (GRCm39) |
Y140H |
probably damaging |
Het |
| Lrp1 |
G |
A |
10: 127,424,046 (GRCm39) |
R809W |
probably damaging |
Het |
| Magi3 |
C |
T |
3: 103,923,384 (GRCm39) |
G1111D |
probably damaging |
Het |
| Mis18bp1 |
A |
T |
12: 65,205,182 (GRCm39) |
V47E |
probably damaging |
Het |
| Mpdz |
T |
C |
4: 81,303,518 (GRCm39) |
E140G |
probably damaging |
Het |
| Myo1d |
T |
C |
11: 80,583,770 (GRCm39) |
I81V |
probably benign |
Het |
| Ntng2 |
C |
A |
2: 29,117,991 (GRCm39) |
E152D |
probably damaging |
Het |
| Or10w1 |
C |
A |
19: 13,632,145 (GRCm39) |
C117* |
probably null |
Het |
| Or5m9b |
T |
C |
2: 85,905,510 (GRCm39) |
L142P |
possibly damaging |
Het |
| Otud3 |
A |
T |
4: 138,629,212 (GRCm39) |
V185D |
probably damaging |
Het |
| Parp6 |
C |
T |
9: 59,538,098 (GRCm39) |
R248W |
probably benign |
Het |
| Pcdhga7 |
A |
G |
18: 37,849,483 (GRCm39) |
T497A |
probably benign |
Het |
| Ppp1r18 |
A |
G |
17: 36,184,769 (GRCm39) |
E140G |
probably damaging |
Het |
| Prl3b1 |
T |
C |
13: 27,431,928 (GRCm39) |
L151P |
probably damaging |
Het |
| Pth1r |
C |
T |
9: 110,551,384 (GRCm39) |
E572K |
possibly damaging |
Het |
| Ptprq |
T |
A |
10: 107,471,199 (GRCm39) |
T1401S |
possibly damaging |
Het |
| Rad51ap2 |
A |
G |
12: 11,507,517 (GRCm39) |
T480A |
possibly damaging |
Het |
| Rax |
T |
A |
18: 66,068,232 (GRCm39) |
Q291L |
unknown |
Het |
| Reck |
T |
C |
4: 43,937,450 (GRCm39) |
L734P |
probably damaging |
Het |
| Rgmb |
A |
G |
17: 16,041,081 (GRCm39) |
F169L |
probably damaging |
Het |
| Rhobtb3 |
T |
C |
13: 76,041,029 (GRCm39) |
I426M |
probably damaging |
Het |
| Ring1 |
C |
A |
17: 34,242,280 (GRCm39) |
A76S |
probably damaging |
Het |
| Robo3 |
T |
G |
9: 37,332,225 (GRCm39) |
Y891S |
probably damaging |
Het |
| Rpusd2 |
A |
G |
2: 118,865,338 (GRCm39) |
I12V |
probably benign |
Het |
| Rragd |
T |
C |
4: 32,995,985 (GRCm39) |
V165A |
possibly damaging |
Het |
| Rsf1 |
G |
GACGGCGGCT |
7: 97,229,116 (GRCm39) |
|
probably benign |
Homo |
| Ryr1 |
G |
T |
7: 28,815,606 (GRCm39) |
Q95K |
probably benign |
Het |
| S1pr4 |
T |
A |
10: 81,334,716 (GRCm39) |
N253Y |
possibly damaging |
Het |
| Scn3a |
A |
C |
2: 65,291,679 (GRCm39) |
V1689G |
probably damaging |
Het |
| Sdhb |
A |
G |
4: 140,700,984 (GRCm39) |
N147D |
probably damaging |
Het |
| Sdk1 |
A |
T |
5: 142,020,181 (GRCm39) |
H913L |
possibly damaging |
Het |
| Serpina3i |
T |
A |
12: 104,232,791 (GRCm39) |
M232K |
probably damaging |
Het |
| Sipa1l2 |
A |
T |
8: 126,201,610 (GRCm39) |
V646E |
probably damaging |
Het |
| Slc38a1 |
A |
T |
15: 96,476,673 (GRCm39) |
I396N |
probably benign |
Het |
| Slc6a13 |
A |
T |
6: 121,279,753 (GRCm39) |
E42D |
probably benign |
Het |
| Stard9 |
G |
A |
2: 120,544,027 (GRCm39) |
V4442M |
probably damaging |
Het |
| Sumo3 |
T |
A |
10: 77,452,071 (GRCm39) |
|
probably benign |
Het |
| Syngap1 |
A |
G |
17: 27,177,104 (GRCm39) |
I356V |
probably benign |
Het |
| Tcam1 |
C |
T |
11: 106,174,880 (GRCm39) |
Q112* |
probably null |
Het |
| Tdp2 |
T |
A |
13: 25,024,378 (GRCm39) |
L225* |
probably null |
Het |
| Tmprss7 |
A |
G |
16: 45,478,485 (GRCm39) |
V747A |
probably benign |
Het |
| Ugt2b34 |
T |
A |
5: 87,054,223 (GRCm39) |
Y186F |
probably benign |
Het |
| Usp37 |
G |
T |
1: 74,514,292 (GRCm39) |
S293* |
probably null |
Het |
| Vmn1r36 |
A |
G |
6: 66,693,230 (GRCm39) |
I109T |
probably benign |
Het |
| Vsig10l |
T |
A |
7: 43,118,396 (GRCm39) |
V798E |
probably benign |
Het |
| Zfp36l1 |
G |
T |
12: 80,159,596 (GRCm39) |
C18* |
probably null |
Het |
| Zfp959 |
T |
C |
17: 56,204,427 (GRCm39) |
Y152H |
probably damaging |
Het |
| Zfyve26 |
A |
T |
12: 79,296,373 (GRCm39) |
C1949S |
probably damaging |
Het |
| Zswim9 |
T |
A |
7: 12,995,529 (GRCm39) |
E209V |
probably damaging |
Het |
|
| Other mutations in Nek10 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01361:Nek10
|
APN |
14 |
14,850,957 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL02067:Nek10
|
APN |
14 |
14,861,639 (GRCm38) |
missense |
probably benign |
0.12 |
|
IGL02361:Nek10
|
APN |
14 |
14,843,856 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02687:Nek10
|
APN |
14 |
14,840,570 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02929:Nek10
|
APN |
14 |
14,821,119 (GRCm38) |
missense |
possibly damaging |
0.82 |
|
IGL03229:Nek10
|
APN |
14 |
14,986,686 (GRCm38) |
missense |
probably benign |
0.10 |
|
P0041:Nek10
|
UTSW |
14 |
14,861,603 (GRCm38) |
missense |
probably benign |
0.01 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0142:Nek10
|
UTSW |
14 |
14,861,560 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R0433:Nek10
|
UTSW |
14 |
14,860,927 (GRCm38) |
missense |
probably benign |
0.32 |
|
R0633:Nek10
|
UTSW |
14 |
14,857,782 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1087:Nek10
|
UTSW |
14 |
14,827,059 (GRCm38) |
missense |
possibly damaging |
0.59 |
|
R1184:Nek10
|
UTSW |
14 |
14,931,325 (GRCm38) |
splice site |
probably benign |
|
|
R1250:Nek10
|
UTSW |
14 |
14,853,887 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1371:Nek10
|
UTSW |
14 |
14,850,983 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1506:Nek10
|
UTSW |
14 |
14,999,078 (GRCm38) |
splice site |
probably benign |
|
|
R1829:Nek10
|
UTSW |
14 |
14,863,454 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1831:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1833:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1990:Nek10
|
UTSW |
14 |
14,860,764 (GRCm38) |
missense |
probably benign |
|
|
R1997:Nek10
|
UTSW |
14 |
14,827,003 (GRCm38) |
missense |
probably benign |
0.09 |
|
R2011:Nek10
|
UTSW |
14 |
14,885,122 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2158:Nek10
|
UTSW |
14 |
14,885,047 (GRCm38) |
splice site |
probably null |
|
|
R2288:Nek10
|
UTSW |
14 |
14,853,956 (GRCm38) |
nonsense |
probably null |
|
|
R2568:Nek10
|
UTSW |
14 |
14,999,112 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R2907:Nek10
|
UTSW |
14 |
14,980,613 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R2965:Nek10
|
UTSW |
14 |
14,836,202 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3922:Nek10
|
UTSW |
14 |
14,861,585 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R4032:Nek10
|
UTSW |
14 |
14,853,877 (GRCm38) |
splice site |
probably null |
|
|
R4700:Nek10
|
UTSW |
14 |
14,842,841 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R4742:Nek10
|
UTSW |
14 |
14,861,624 (GRCm38) |
missense |
probably null |
0.03 |
|
R4785:Nek10
|
UTSW |
14 |
14,855,714 (GRCm38) |
missense |
probably benign |
|
|
R4890:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4891:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4920:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4924:Nek10
|
UTSW |
14 |
14,846,594 (GRCm38) |
splice site |
probably null |
|
|
R4928:Nek10
|
UTSW |
14 |
14,930,577 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4948:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4952:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4953:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R5092:Nek10
|
UTSW |
14 |
14,820,851 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R5097:Nek10
|
UTSW |
14 |
14,857,851 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5593:Nek10
|
UTSW |
14 |
14,980,544 (GRCm38) |
nonsense |
probably null |
|
|
R5696:Nek10
|
UTSW |
14 |
14,860,736 (GRCm38) |
splice site |
probably null |
|
|
R5813:Nek10
|
UTSW |
14 |
14,986,704 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5829:Nek10
|
UTSW |
14 |
14,865,404 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5872:Nek10
|
UTSW |
14 |
14,850,896 (GRCm38) |
missense |
probably benign |
0.06 |
|
R5939:Nek10
|
UTSW |
14 |
14,931,290 (GRCm38) |
missense |
possibly damaging |
0.58 |
|
R6025:Nek10
|
UTSW |
14 |
14,865,633 (GRCm38) |
missense |
probably benign |
0.41 |
|
R6539:Nek10
|
UTSW |
14 |
14,860,789 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R6542:Nek10
|
UTSW |
14 |
14,999,108 (GRCm38) |
missense |
probably benign |
0.44 |
|
R6561:Nek10
|
UTSW |
14 |
14,828,448 (GRCm38) |
missense |
possibly damaging |
0.48 |
|
R6659:Nek10
|
UTSW |
14 |
14,861,684 (GRCm38) |
missense |
probably benign |
0.29 |
|
R7039:Nek10
|
UTSW |
14 |
14,986,700 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7039:Nek10
|
UTSW |
14 |
14,826,946 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R7102:Nek10
|
UTSW |
14 |
14,828,517 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7185:Nek10
|
UTSW |
14 |
14,846,621 (GRCm38) |
missense |
probably benign |
0.03 |
|
R7198:Nek10
|
UTSW |
14 |
14,850,947 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7202:Nek10
|
UTSW |
14 |
14,836,171 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7251:Nek10
|
UTSW |
14 |
14,853,965 (GRCm38) |
missense |
probably benign |
|
|
R7345:Nek10
|
UTSW |
14 |
14,955,503 (GRCm38) |
missense |
probably benign |
|
|
R7590:Nek10
|
UTSW |
14 |
15,006,693 (GRCm38) |
makesense |
probably null |
|
|
R7593:Nek10
|
UTSW |
14 |
14,826,955 (GRCm38) |
missense |
probably benign |
0.04 |
|
R7616:Nek10
|
UTSW |
14 |
14,937,759 (GRCm38) |
missense |
probably benign |
0.27 |
|
R7635:Nek10
|
UTSW |
14 |
14,850,932 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7817:Nek10
|
UTSW |
14 |
15,001,017 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7826:Nek10
|
UTSW |
14 |
14,860,846 (GRCm38) |
splice site |
probably null |
|
|
R7986:Nek10
|
UTSW |
14 |
15,001,020 (GRCm38) |
missense |
probably benign |
0.17 |
|
R8765:Nek10
|
UTSW |
14 |
14,999,104 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R8856:Nek10
|
UTSW |
14 |
14,937,610 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8973:Nek10
|
UTSW |
14 |
14,931,321 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9002:Nek10
|
UTSW |
14 |
14,980,590 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9088:Nek10
|
UTSW |
14 |
14,931,314 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9195:Nek10
|
UTSW |
14 |
14,821,139 (GRCm38) |
missense |
probably benign |
0.03 |
|
R9464:Nek10
|
UTSW |
14 |
14,937,766 (GRCm38) |
missense |
probably benign |
|
|
R9511:Nek10
|
UTSW |
14 |
14,828,511 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9529:Nek10
|
UTSW |
14 |
14,850,833 (GRCm38) |
missense |
probably benign |
|
|
R9590:Nek10
|
UTSW |
14 |
14,853,888 (GRCm38) |
missense |
probably damaging |
1.00 |
|
Z1177:Nek10
|
UTSW |
14 |
15,001,157 (GRCm38) |
nonsense |
probably null |
|
|
Z1177:Nek10
|
UTSW |
14 |
14,853,948 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CAGTGGCATTAGGTAGGTTTAAC -3'
(R):5'- CTGGATGCCAACCAAGTCTTG -3'
Sequencing Primer
(F):5'- AGGTTTAACGCTTTTGTGAGAAATTG -3'
(R):5'- CTGACCCAGGTGAGAGCAG -3'
|
| Posted On |
2018-02-28 |
Incidental Mutation