Mutagenetix > Incidental Mutation

Incidental Mutation 'R6249:Olfr169'

505879

Institutional Source

Beutler Lab

Gene Symbol

Olfr169

Ensembl Gene

ENSMUSG00000068535

Gene Name

olfactory receptor 169

Synonyms

GA_x54KRFPKG5P-16014972-16014031, MOR273-3P

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R6249 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19564889-19571809 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19565975 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Tyrosine at position 303 (N303Y)

Ref Sequence

ENSEMBL: ENSMUSP00000150528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090062] [ENSMUST00000215040] [ENSMUST00000215476] [ENSMUST00000216070]

AlphaFold

Q7TS53

Predicted Effect

probably damaging
Transcript: ENSMUST00000090062
AA Change: N303Y

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000087516
Gene: ENSMUSG00000068535
AA Change: N303Y

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	308	1.3e-45	PFAM
Pfam:7TM_GPCR_Srsx	35	303	2e-6	PFAM
Pfam:7tm_1	41	290	7.4e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215040
AA Change: N303Y

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215476
AA Change: N303Y

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000216070
AA Change: N303Y

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 95.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110004F10Rik	A	T	7: 116,103,270	S90C	probably damaging	Het
Abca9	T	C	11: 110,145,627	D552G	probably benign	Het
Adgrb3	A	G	1: 25,432,558	L502P	probably damaging	Het
Agmo	T	C	12: 37,243,838		probably null	Het
Ank3	A	G	10: 69,823,076		probably null	Het
Apol11b	G	T	15: 77,635,337	T181K	probably benign	Het
Arap2	G	T	5: 62,646,193	H1244N	probably damaging	Het
Arid1b	A	T	17: 5,279,361	M838L	possibly damaging	Het
Aste1	G	A	9: 105,396,617	V19I	probably benign	Het
B3gnt3	A	T	8: 71,692,662	M354K	probably damaging	Het
Calcr	A	T	6: 3,692,711	M381K	possibly damaging	Het
Ccdc63	A	C	5: 122,124,999	L160W	probably benign	Het
Cerkl	A	G	2: 79,368,778	L156P	probably damaging	Het
Dab1	C	T	4: 104,731,751	A524V	probably benign	Het
Ddb1	C	A	19: 10,605,720	Y5*	probably null	Het
Fam205a1	A	T	4: 42,850,528	W543R	probably benign	Het
Fignl2	T	C	15: 101,054,179	E74G	possibly damaging	Het
Glb1l2	C	T	9: 26,765,554		probably benign	Het
Gm10031	A	G	1: 156,525,230	M334V	probably benign	Het
Gm16503	A	G	4: 147,541,051	M1V	probably null	Het
Gm2696	A	C	10: 77,794,812		probably benign	Het
Gm45844	A	G	7: 7,240,093	S20P	probably benign	Het
Gm7579	C	T	7: 142,212,006	P50S	unknown	Het
Idh1	A	G	1: 65,166,219	S196P	probably damaging	Het
Il1rap	T	C	16: 26,692,848	V214A	possibly damaging	Het
Kctd17	C	G	15: 78,430,039		probably null	Het
Lama1	G	T	17: 67,798,604	V2036L	probably benign	Het
Lars	G	T	18: 42,257,206		probably null	Het
Mdn1	T	A	4: 32,708,484	V1670E	possibly damaging	Het
Mtmr7	A	T	8: 40,581,482	I266N	probably damaging	Het
Ncapd3	GGCTGCTGCTGCTGCTGCTGCTG	GGCTGCTGCTGCTGCTGCTG	9: 27,088,053		probably benign	Het
Nckap5	T	C	1: 126,024,930	E1295G	probably benign	Het
Nrxn3	T	A	12: 89,254,678	V409D	probably damaging	Het
Olfr1341	A	G	4: 118,709,713	Y102C	probably damaging	Het
Olfr190	T	C	16: 59,074,432	Y216C	probably damaging	Het
Olfr448	G	T	6: 42,897,304	L284F	probably damaging	Het
Olfr635	A	T	7: 103,979,611	S140C	possibly damaging	Het
Olfr907	T	G	9: 38,499,584	I305R	unknown	Het
Pcdha4	T	A	18: 36,953,676	V304E	probably damaging	Het
Pde6c	T	C	19: 38,158,560		probably null	Het
Pebp4	A	T	14: 70,059,650	T213S	possibly damaging	Het
Phf2	C	A	13: 48,805,872	R886L	unknown	Het
Pik3ca	A	T	3: 32,461,563	H795L	probably damaging	Het
Pisd	G	T	5: 32,738,844	T379N	probably damaging	Het
Pld2	T	C	11: 70,555,370	L732P	probably damaging	Het
Pskh1	A	G	8: 105,912,985	D99G	possibly damaging	Het
Rest	A	G	5: 77,281,224	T497A	probably benign	Het
Rps24	C	T	14: 24,493,462	T108M	possibly damaging	Het
Rtel1	C	T	2: 181,351,682	R566C	probably damaging	Het
Scyl2	A	G	10: 89,657,857	S350P	possibly damaging	Het
Slc38a7	G	T	8: 95,837,674		probably null	Het
Sox5	A	G	6: 143,833,283	I674T	probably benign	Het
Surf4	C	A	2: 26,926,887	E39D	probably damaging	Het
Tas2r144	T	A	6: 42,215,357	Y10*	probably null	Het
Thbs4	C	T	13: 92,774,707	G323R	probably damaging	Het
Top2b	A	G	14: 16,399,006	Y542C	probably damaging	Het
Trim61	A	G	8: 65,014,456	V51A	probably benign	Het
Tyrp1	T	A	4: 80,850,772	I501N	possibly damaging	Het
U2surp	C	T	9: 95,500,816	D32N	probably benign	Het
Ush1c	T	C	7: 46,214,959	Y411C	probably damaging	Het
Vwa7	G	A	17: 35,023,389	V490I	probably benign	Het
Xylt1	G	T	7: 117,667,305	A849S	probably benign	Het
Ythdc1	T	A	5: 86,831,956	N599K	possibly damaging	Het
Zbtb38	T	A	9: 96,685,992	Y1013F	probably damaging	Het
Zfp709	TCGACG	TCG	8: 71,890,708		probably benign	Het
Zswim9	T	C	7: 13,260,977	S418G	probably damaging	Het

Other mutations in Olfr169

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01080:Olfr169	APN	16	19566208	missense	probably damaging	1.00
IGL01862:Olfr169	APN	16	19566676	missense	probably damaging	1.00
IGL02064:Olfr169	APN	16	19566548	missense	probably damaging	1.00
IGL03061:Olfr169	APN	16	19566713	missense	possibly damaging	0.87
IGL03136:Olfr169	APN	16	19566353	missense	probably damaging	1.00
R0066:Olfr169	UTSW	16	19566049	missense	probably damaging	1.00
R0243:Olfr169	UTSW	16	19566294	missense	probably damaging	0.97
R0629:Olfr169	UTSW	16	19565980	missense	possibly damaging	0.88
R1644:Olfr169	UTSW	16	19566406	missense	probably benign	0.11
R1943:Olfr169	UTSW	16	19566437	missense	probably benign	0.19
R3016:Olfr169	UTSW	16	19566391	missense	probably damaging	1.00
R4290:Olfr169	UTSW	16	19566244	missense	possibly damaging	0.88
R4689:Olfr169	UTSW	16	19566513	nonsense	probably null
R4791:Olfr169	UTSW	16	19566663	missense	possibly damaging	0.50
R5497:Olfr169	UTSW	16	19566330	missense	probably benign	0.10
R5843:Olfr169	UTSW	16	19566583	missense	probably damaging	1.00
R6106:Olfr169	UTSW	16	19566259	missense	probably damaging	0.99
R7895:Olfr169	UTSW	16	19566722	nonsense	probably null
R9284:Olfr169	UTSW	16	19566607	missense	probably damaging	1.00
R9335:Olfr169	UTSW	16	19566763	missense	probably benign	0.32
R9364:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68
R9404:Olfr169	UTSW	16	19565981	missense	probably benign	0.01
R9475:Olfr169	UTSW	16	19566520	missense	probably benign	0.09
R9554:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- AGACTTTACCATGGGCATCTG -3'
(R):5'- TCCAAATGCAGTCATCAGGG -3'

Sequencing Primer
(F):5'- ACCATGGGCATCTGTATACAG -3'
(R):5'- GGCCCGGCAAAAGTCCTTTTC -3'

Posted On

2018-02-28