Mutagenetix > Incidental Mutation

Incidental Mutation 'R6255:Tspan10'

506069

Institutional Source

Beutler Lab

Gene Symbol

Tspan10

Ensembl Gene

ENSMUSG00000039691

Gene Name

tetraspanin 10

Synonyms

Ocsp

MMRRC Submission

044372-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.237) question?

Stock #

R6255 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

120442644-120446950 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 120444542 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 159 (C159*)

Ref Sequence

ENSEMBL: ENSMUSP00000041883 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026452] [ENSMUST00000044105]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000026452

SMART Domains

Protein: ENSMUSP00000026452
Gene: ENSMUSG00000025386

Domain	Start	End	E-Value	Type
Pfam:PDE6_gamma	6	87	6e-51	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000044105
AA Change: C159*

SMART Domains

Protein: ENSMUSP00000041883
Gene: ENSMUSG00000039691
AA Change: C159*

Domain	Start	End	E-Value	Type
Pfam:Tetraspannin	74	318	1.9e-42	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141795

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143192

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155421

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.8%

Validation Efficiency

100% (58/58)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	T	A	13: 119,466,123 (GRCm38)	V7E	possibly damaging	Het
Aars2	G	A	17: 45,514,609 (GRCm38)	G333S	probably damaging	Het
Aen	T	A	7: 78,905,844 (GRCm38)	I85N	probably damaging	Het
Ahnak	C	A	19: 9,008,025 (GRCm38)	H2224Q	possibly damaging	Het
Aldh18a1	C	T	19: 40,580,043 (GRCm38)	R41H	possibly damaging	Het
Bpifb9b	T	A	2: 154,309,364 (GRCm38)	W2R	probably damaging	Het
Caprin2	A	G	6: 148,877,892 (GRCm38)	I139T	probably benign	Het
Cdhr3	T	A	12: 33,053,475 (GRCm38)	N381I	probably damaging	Het
Cecr2	A	G	6: 120,758,050 (GRCm38)	Y721C	probably damaging	Het
Cfhr4	A	T	1: 139,753,011 (GRCm38)	C256*	probably null	Het
Cherp	G	T	8: 72,470,881 (GRCm38)	A125D	probably damaging	Het
Cped1	G	A	6: 22,138,715 (GRCm38)		probably null	Het
Ctdp1	T	A	18: 80,459,297 (GRCm38)		probably null	Het
Cyp2c55	T	C	19: 39,018,667 (GRCm38)	I169T	probably benign	Het
Cyp4a31	T	C	4: 115,574,920 (GRCm38)	L418P	possibly damaging	Het
Efcab7	T	C	4: 99,829,390 (GRCm38)		probably benign	Het
Efcab8	T	C	2: 153,810,268 (GRCm38)	W466R	possibly damaging	Het
Ehd3	C	A	17: 73,805,413 (GRCm38)	N57K	probably benign	Het
Ern2	C	A	7: 122,173,272 (GRCm38)	K654N	probably damaging	Het
Fbh1	A	T	2: 11,748,446 (GRCm38)	F879L	probably benign	Het
Gde1	T	C	7: 118,691,781 (GRCm38)	D92G	probably null	Het
Heatr5b	A	G	17: 78,803,434 (GRCm38)	V995A	probably damaging	Het
Ifrd2	A	G	9: 107,592,091 (GRCm38)	E346G	probably damaging	Het
Ism1	AACGGACCCGTTCTTGTGGCTATGCA	AA	2: 139,746,042 (GRCm38)		probably benign	Het
Itgb4	T	C	11: 115,998,137 (GRCm38)	V1102A	possibly damaging	Het
Itgb6	A	T	2: 60,605,276 (GRCm38)	I710N	probably damaging	Het
Kif1a	T	C	1: 93,019,983 (GRCm38)	K1578E	probably damaging	Het
Kif9	T	C	9: 110,517,834 (GRCm38)		probably null	Het
Kitl	T	C	10: 100,089,233 (GRCm38)	*57Q	probably null	Het
Lrat	C	A	3: 82,903,505 (GRCm38)	V70F	probably damaging	Het
Lrrc9	T	A	12: 72,487,023 (GRCm38)	M1022K	probably benign	Het
Muc16	T	C	9: 18,655,599 (GRCm38)	T1875A	unknown	Het
Mup4	T	A	4: 59,957,890 (GRCm38)	N171I	probably damaging	Het
Npas4	G	A	19: 4,986,375 (GRCm38)	T587I	probably damaging	Het
Oas3	A	G	5: 120,771,230 (GRCm38)	V217A	probably benign	Het
Or6c1b	A	G	10: 129,437,688 (GRCm38)	N292S	possibly damaging	Het
Osbp	C	A	19: 11,977,953 (GRCm38)	A323D	possibly damaging	Het
Panx2	G	A	15: 89,067,618 (GRCm38)	R96H	probably damaging	Het
Pcdhb18	G	A	18: 37,490,484 (GRCm38)	R289Q	probably benign	Het
Piezo2	T	C	18: 63,121,270 (GRCm38)	R385G	possibly damaging	Het
Pkn2	T	C	3: 142,811,599 (GRCm38)	T476A	probably damaging	Het
Plekha4	T	C	7: 45,553,802 (GRCm38)		probably benign	Het
Ppfibp2	T	C	7: 107,681,762 (GRCm38)	S94P	probably damaging	Het
Pramel7	T	A	2: 87,489,663 (GRCm38)	I429L	probably benign	Het
Rif1	A	G	2: 52,085,053 (GRCm38)	K325E	probably damaging	Het
Ror2	T	C	13: 53,110,542 (GRCm38)	Y826C	probably damaging	Het
Rsph10b	G	A	5: 143,959,746 (GRCm38)	G19R	probably damaging	Het
Slc20a1	A	G	2: 129,208,004 (GRCm38)	N361D	probably damaging	Het
Slc26a9	A	G	1: 131,763,909 (GRCm38)	D630G	probably benign	Het
Smtnl2	G	A	11: 72,401,399 (GRCm38)	A274V	probably damaging	Het
Trank1	T	C	9: 111,352,246 (GRCm38)		probably null	Het
Uba6	G	A	5: 86,164,765 (GRCm38)	T23I	probably benign	Het
Vmn2r74	T	C	7: 85,952,451 (GRCm38)	T660A	possibly damaging	Het
Vwa5b1	T	C	4: 138,578,672 (GRCm38)	N905S	probably benign	Het
Zfp831	T	C	2: 174,646,421 (GRCm38)	L963P	possibly damaging	Het
Zfp990	T	A	4: 145,537,789 (GRCm38)	N452K	probably benign	Het

Other mutations in Tspan10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00848:Tspan10	APN	11	120,444,270 (GRCm38)	missense	probably benign	0.00
IGL02219:Tspan10	APN	11	120,446,372 (GRCm38)	missense	probably benign	0.16
R0427:Tspan10	UTSW	11	120,444,294 (GRCm38)	missense	probably damaging	1.00
R0551:Tspan10	UTSW	11	120,444,418 (GRCm38)	missense	probably damaging	1.00
R1688:Tspan10	UTSW	11	120,442,782 (GRCm38)	missense	probably damaging	0.97
R2209:Tspan10	UTSW	11	120,446,163 (GRCm38)	missense	probably benign	0.02
R4657:Tspan10	UTSW	11	120,444,498 (GRCm38)	missense	probably damaging	0.99
R4767:Tspan10	UTSW	11	120,446,166 (GRCm38)	missense	probably damaging	1.00
R5556:Tspan10	UTSW	11	120,444,715 (GRCm38)	missense	possibly damaging	0.96
R6873:Tspan10	UTSW	11	120,444,723 (GRCm38)	missense	probably damaging	1.00
R6959:Tspan10	UTSW	11	120,444,696 (GRCm38)	missense	probably damaging	1.00
R9249:Tspan10	UTSW	11	120,446,225 (GRCm38)	missense	probably benign	0.11
X0065:Tspan10	UTSW	11	120,446,265 (GRCm38)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GGGGCCTGACTGTTAAAAGG -3'
(R):5'- TCTTACCCACGTGGCGTTTG -3'

Sequencing Primer
(F):5'- GCCTGACTGTTAAAAGGTCTCAG -3'
(R):5'- GGACACTCACAGGTTCTGTTGC -3'

Posted On

2018-02-28