Mutagenetix > Incidental Mutations

Incidental Mutation 'R6263:Vmn2r32'

506821

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r32

Ensembl Gene

ENSMUSG00000096743

Gene Name

vomeronasal 2, receptor 32

Synonyms

V2r5

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.215) question?

Stock #

R6263 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

7463015-7479973 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 7476692 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 161 (S161T)

Ref Sequence

ENSEMBL: ENSMUSP00000092462 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094866]

AlphaFold

K7N686

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094866
AA Change: S161T

PolyPhen 2 Score 0.903 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000092462
Gene: ENSMUSG00000096743
AA Change: S161T

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	3.6e-33	PFAM
Pfam:NCD3G	512	565	8.1e-21	PFAM
Pfam:7tm_3	598	833	9.5e-55	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.7%

Validation Efficiency

97% (66/68)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931423N10Rik	T	A	2: 23,266,733		probably benign	Het
4932438A13Rik	T	A	3: 36,931,111	N863K	probably benign	Het
Ahnak	A	G	19: 9,018,277	I5642V	probably benign	Het
Atp5a1	A	G	18: 77,779,230		probably null	Het
Bsn	A	T	9: 108,113,254	F1766L	probably damaging	Het
Ccdc141	T	C	2: 77,108,463	Q266R	probably damaging	Het
Cdh10	T	A	15: 18,964,068	D105E	possibly damaging	Het
Clca3a1	T	A	3: 144,749,778	D418V	probably damaging	Het
Cnnm2	C	A	19: 46,856,905	T612K	probably benign	Het
Col3a1	T	C	1: 45,321,575	V55A	unknown	Het
Cpd	A	C	11: 76,846,271	D232E	probably benign	Het
Csde1	A	G	3: 103,040,017	H95R	probably benign	Het
Ctcfl	T	A	2: 173,095,337	H596L	probably benign	Het
Cwc22	T	C	2: 77,896,171	R855G	possibly damaging	Het
Cwc25	A	G	11: 97,753,227	Y227H	probably damaging	Het
Cyp2c38	G	A	19: 39,392,215	P409S	probably damaging	Het
Ddx5	A	G	11: 106,788,313	S2P	possibly damaging	Het
Dnah2	C	A	11: 69,457,412	G2570W	probably damaging	Het
Dnajc10	T	A	2: 80,343,948	V528E	probably damaging	Het
Fmo1	A	G	1: 162,850,060		probably null	Het
Gm11639	G	A	11: 104,919,486	D3150N	unknown	Het
Gpatch1	C	A	7: 35,303,423	D221Y	probably damaging	Het
Ino80	T	C	2: 119,383,414	Y1225C	probably damaging	Het
Itga7	T	A	10: 128,944,086	D501E	probably benign	Het
Lgr4	T	C	2: 110,011,898	S743P	possibly damaging	Het
Lilra5	A	T	7: 4,238,361	Y99F	probably damaging	Het
Lmna	A	G	3: 88,502,958	V49A	probably damaging	Het
Lrrc72	G	A	12: 36,208,604	R267*	probably null	Het
Mapkbp1	T	C	2: 120,023,291	S1199P	probably damaging	Het
Mmp25	G	A	17: 23,630,794	A541V	possibly damaging	Het
Myh10	A	T	11: 68,810,232	N1756Y	probably damaging	Het
Nfe2	A	G	15: 103,250,951	I31T	probably damaging	Het
Nfe2l1	A	T	11: 96,817,744	F732I	probably benign	Het
Olfr1238	C	G	2: 89,406,730	M116I	possibly damaging	Het
Olfr1255	T	A	2: 89,816,760	C145S	probably damaging	Het
Olfr603	C	A	7: 103,383,196	V269F	possibly damaging	Het
Pax6	T	C	2: 105,692,854		probably null	Het
Phb	A	G	11: 95,678,115	E192G	probably damaging	Het
Plekhn1	A	T	4: 156,225,193		probably null	Het
Plxnb2	A	T	15: 89,161,986	V942E	probably damaging	Het
Rsrc2	G	A	5: 123,739,688		probably benign	Het
Sept8	G	A	11: 53,548,383	C460Y	probably benign	Het
Slc13a2	CGTTATCTGT	CGT	11: 78,403,480		probably benign	Het
Slc16a7	A	G	10: 125,294,639	I59T	probably benign	Het
Slc35d1	A	T	4: 103,208,168	I172N	possibly damaging	Het
Smg5	A	T	3: 88,341,901	N40Y	possibly damaging	Het
Smurf1	A	G	5: 144,881,731	V633A	probably damaging	Het
Snx9	T	C	17: 5,887,049	V22A	probably damaging	Het
Sox6	A	T	7: 115,477,060	M741K	probably damaging	Het
Spata31d1d	T	C	13: 59,725,983	Q1246R	probably benign	Het
Sval1	A	G	6: 41,951,726	E24G	probably damaging	Het
Tas2r124	A	G	6: 132,754,904	I59V	probably benign	Het
Tmem151b	T	A	17: 45,547,066	T85S	probably benign	Het
Tpr	T	A	1: 150,442,245		probably null	Het
Trpm6	A	C	19: 18,854,108	T1446P	possibly damaging	Het
Tsc22d4	A	T	5: 137,768,179	K502N	possibly damaging	Het
Tssk3	C	T	4: 129,489,258	S207N	probably benign	Het
Ttbk1	G	T	17: 46,467,262	P618Q	probably damaging	Het
Ttll6	A	T	11: 96,156,545	M657L	probably benign	Het
Ubash3a	T	A	17: 31,215,095	I138N	probably benign	Het
Ush2a	A	G	1: 188,358,642	Y490C	probably damaging	Het
Vmn1r82	G	T	7: 12,305,534	V127F	probably damaging	Het
Wdr49	T	C	3: 75,481,517	I58M	possibly damaging	Het
Wiz	A	T	17: 32,360,443		probably null	Het
Wrap53	A	T	11: 69,562,793	Y324*	probably null	Het
Zfp747	A	C	7: 127,375,966		probably benign	Het
Zfp827	A	G	8: 79,179,073	Y33C	probably damaging	Het

Other mutations in Vmn2r32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02140:Vmn2r32	APN	7	7476697	missense	probably damaging	0.97
IGL02333:Vmn2r32	APN	7	7464144	missense	probably damaging	1.00
IGL02406:Vmn2r32	APN	7	7476710	missense	probably benign
IGL02428:Vmn2r32	APN	7	7474284	missense	probably benign	0.02
IGL02484:Vmn2r32	APN	7	7464117	missense	probably damaging	1.00
IGL03277:Vmn2r32	APN	7	7474252	missense	probably benign	0.23
IGL03366:Vmn2r32	APN	7	7464030	missense	probably damaging	0.99
R1055:Vmn2r32	UTSW	7	7474327	nonsense	probably null
R1695:Vmn2r32	UTSW	7	7463992	missense	probably benign	0.01
R2172:Vmn2r32	UTSW	7	7474615	missense	probably damaging	0.99
R2262:Vmn2r32	UTSW	7	7474619	missense	probably benign
R3150:Vmn2r32	UTSW	7	7472555	missense	probably benign
R4362:Vmn2r32	UTSW	7	7479858	nonsense	probably null
R4432:Vmn2r32	UTSW	7	7479919	missense	probably damaging	0.98
R4851:Vmn2r32	UTSW	7	7479954	missense	possibly damaging	0.59
R4949:Vmn2r32	UTSW	7	7464084	missense	probably benign	0.22
R5990:Vmn2r32	UTSW	7	7479810	missense	probably damaging	0.97
R6083:Vmn2r32	UTSW	7	7464210	missense	probably benign	0.15
R6084:Vmn2r32	UTSW	7	7464210	missense	probably benign	0.15
R6116:Vmn2r32	UTSW	7	7464093	missense	probably damaging	1.00
R6889:Vmn2r32	UTSW	7	7472574	missense	possibly damaging	0.82
R7286:Vmn2r32	UTSW	7	7479808	missense	probably benign	0.21
R7390:Vmn2r32	UTSW	7	7479852	missense	probably benign	0.00
R7412:Vmn2r32	UTSW	7	7474213	missense	possibly damaging	0.53
R7508:Vmn2r32	UTSW	7	7467374	missense	possibly damaging	0.87
R8812:Vmn2r32	UTSW	7	7474670	missense	probably damaging	1.00
R8968:Vmn2r32	UTSW	7	7474205	missense	probably benign	0.00
R9331:Vmn2r32	UTSW	7	7464403	nonsense	probably null
R9358:Vmn2r32	UTSW	7	7474198	missense	probably damaging	1.00
Z1177:Vmn2r32	UTSW	7	7474161	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GCCTGACTTAATTCTACATGGC -3'
(R):5'- CAGATACTCTTTGGGCCATTGTG -3'

Sequencing Primer
(F):5'- ATCTGTCAACTTCTGGTGGTAGTAAC -3'
(R):5'- GTGATGGAAAAACTGTAACACCTAC -3'

Posted On

2018-03-15