Mutagenetix > Incidental Mutation

Incidental Mutation 'R6265:Tas2r103'

506942

Institutional Source

Beutler Lab

Gene Symbol

Tas2r103

Ensembl Gene

ENSMUSG00000030196

Gene Name

taste receptor, type 2, member 103

Synonyms

mt2r63, T2R3, Tas2r10, EG667992, mGR03, TRB2, Tas2r3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R6265 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

133036163-133037101 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 133036531 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Valine at position 191 (F191V)

Ref Sequence

ENSEMBL: ENSMUSP00000032317 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032317]

AlphaFold

Q9JKA3

Predicted Effect

probably damaging
Transcript: ENSMUST00000032317
AA Change: F191V

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000032317
Gene: ENSMUSG00000030196
AA Change: F191V

Domain	Start	End	E-Value	Type
Pfam:TAS2R	5	301	1.5e-79	PFAM

Meta Mutation Damage Score

0.4903

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 95.9%

Validation Efficiency

100% (65/65)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700013G24Rik	C	T	4: 137,454,675	P47L	probably damaging	Het
Acot1	T	C	12: 84,016,913	I265T	probably benign	Het
Amotl1	T	C	9: 14,571,655	D587G	possibly damaging	Het
Anks1b	T	G	10: 90,941,500	D1117E	probably damaging	Het
Art4	T	G	6: 136,854,888	N85T	probably damaging	Het
Asb3	C	A	11: 31,085,143	Q462K	probably benign	Het
Atcay	C	T	10: 81,213,280	E163K	possibly damaging	Het
Atp6v0e	T	C	17: 26,676,533	V20A	possibly damaging	Het
Atp7b	G	A	8: 22,015,927	Q520*	probably null	Het
Baat	A	T	4: 49,502,836	D95E	possibly damaging	Het
Cars2	TCCCC	TCCC	8: 11,529,599		probably null	Het
Ccdc24	G	A	4: 117,871,177	Q47*	probably null	Het
Cep170b	C	A	12: 112,744,559	Q1488K	probably damaging	Het
Cldn24	A	C	8: 47,822,339	D66A	probably benign	Het
Cln5	C	A	14: 103,073,227	T110K	probably damaging	Het
Clvs2	C	A	10: 33,528,515	S235I	possibly damaging	Het
Col5a3	C	T	9: 20,793,764	G730R	unknown	Het
Crim1	C	T	17: 78,370,085	P905L	probably benign	Het
Cxxc4	T	C	3: 134,258,063	V356A	probably benign	Het
Dctn6	G	T	8: 34,094,903	N93K	probably damaging	Het
Dnah9	G	A	11: 66,168,094	A125V	probably benign	Het
Dnhd1	T	C	7: 105,693,370	I1307T	probably benign	Het
Dpy19l1	T	A	9: 24,432,371	I493F	possibly damaging	Het
Ebf2	A	G	14: 67,424,060	I546V	probably benign	Het
Fbll1	T	C	11: 35,797,809	E209G	probably damaging	Het
Foxj2	G	A	6: 122,828,174	A2T	probably damaging	Het
Foxs1	A	T	2: 152,932,178	C318*	probably null	Het
Gorab	T	C	1: 163,386,630	T244A	possibly damaging	Het
Gtsf1l	C	A	2: 163,087,663		probably benign	Het
Herc1	T	C	9: 66,372,016	S69P	probably benign	Het
Hpca	C	T	4: 129,118,652	W30*	probably null	Het
Ing3	C	A	6: 21,953,814	Q85K	probably damaging	Het
Klhdc8b	T	C	9: 108,448,425	E264G	probably damaging	Het
Lair1	T	A	7: 4,055,827		probably benign	Het
Lama1	T	C	17: 67,750,655	Y575H	probably damaging	Het
Lamtor2	C	A	3: 88,550,713	G29*	probably null	Het
Loxhd1	A	T	18: 77,361,730	D341V	probably damaging	Het
Lrp2	T	A	2: 69,466,340	D3290V	probably damaging	Het
Matn2	A	T	15: 34,399,155	D396V	probably damaging	Het
Me3	T	A	7: 89,849,743	D510E	probably benign	Het
Melk	A	G	4: 44,318,109	Y170C	probably damaging	Het
Mgat2	T	C	12: 69,184,793	V47A	probably benign	Het
Mindy4	T	C	6: 55,301,064	I631T	probably damaging	Het
Myo5b	T	A	18: 74,577,440		probably null	Het
Myo7b	A	T	18: 31,998,150	D521E	probably damaging	Het
Nlrp9b	T	A	7: 20,062,683	F986I	probably benign	Het
Olfr1089	A	G	2: 86,732,955	L219P	probably damaging	Het
Patj	A	G	4: 98,469,567	D690G	probably benign	Het
Pias2	T	C	18: 77,097,258	S5P	probably damaging	Het
Reep4	A	T	14: 70,547,703	S150C	probably damaging	Het
Slc25a21	T	C	12: 57,196,900	R14G	probably benign	Het
Slc6a6	G	C	6: 91,754,915	R575T	probably damaging	Het
Speg	T	A	1: 75,406,679	Y886*	probably null	Het
Tbc1d19	A	G	5: 53,837,924	D145G	probably benign	Het
Tex47	T	A	5: 7,305,461	I214N	probably damaging	Het
Tnnt3	A	T	7: 142,501,645	D3V	probably damaging	Het
Trdv2-1	T	G	14: 53,946,385	S24A	probably benign	Het
Ubr4	A	G	4: 139,452,640	D3377G	possibly damaging	Het
Ugt3a2	A	T	15: 9,361,579	D147V	probably damaging	Het
Usp9y	A	T	Y: 1,446,843	D103E	probably benign	Homo
Vmn1r168	A	G	7: 23,541,536	I273V	probably benign	Het
Vmn2r61	T	C	7: 42,266,491	I176T	probably benign	Het
Vmn2r8	T	A	5: 108,808,597	D53V	probably benign	Het
Zbtb8os	A	T	4: 129,335,982		probably benign	Het
Zfp551	C	T	7: 12,415,412	R690Q	probably damaging	Het

Other mutations in Tas2r103

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Tas2r103	APN	6	133037056	missense	possibly damaging	0.86
IGL02332:Tas2r103	APN	6	133036512	missense	probably benign	0.21
IGL03122:Tas2r103	APN	6	133036909	missense	probably damaging	1.00
IGL03167:Tas2r103	APN	6	133036660	missense	probably damaging	0.98
R0380:Tas2r103	UTSW	6	133036203	missense	probably damaging	1.00
R0671:Tas2r103	UTSW	6	133036350	missense	probably benign	0.01
R1700:Tas2r103	UTSW	6	133036811	missense	probably damaging	1.00
R2098:Tas2r103	UTSW	6	133036597	missense	probably benign
R3977:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3979:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3980:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R4924:Tas2r103	UTSW	6	133036198	missense	probably benign	0.02
R5779:Tas2r103	UTSW	6	133036945	missense	probably benign	0.02
R5839:Tas2r103	UTSW	6	133036816	missense	probably benign	0.06
R7768:Tas2r103	UTSW	6	133036849	missense	probably benign	0.06
R8851:Tas2r103	UTSW	6	133036933	missense
R9435:Tas2r103	UTSW	6	133036723	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGGGAAAAGCTACTCCTATACTCC -3'
(R):5'- AAAGTGGTATCAGTGACATTGGTG -3'

Sequencing Primer
(F):5'- TTCAAAGACTCTGAAAGAAGTGAC -3'
(R):5'- GACATTGGTGGTATCTGTGATAATC -3'

Posted On

2018-03-15