Mutagenetix > Incidental Mutation

Incidental Mutation 'R6267:Myo5b'

507123

Institutional Source

Beutler Lab

Gene Symbol

Myo5b

Ensembl Gene

ENSMUSG00000025885

Gene Name

myosin VB

Synonyms

MMRRC Submission

044405-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.752) question?

Stock #

R6267 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

74440936-74771493 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 74616991 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 173 (Y173C)

Ref Sequence

ENSEMBL: ENSMUSP00000123123 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074157] [ENSMUST00000121875] [ENSMUST00000125882]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000074157
AA Change: Y175C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000073790
Gene: ENSMUSG00000025885
AA Change: Y175C

Domain	Start	End	E-Value	Type
MYSc	63	763	N/A	SMART
IQ	764	786	2.41e-4	SMART
IQ	787	809	7.7e-3	SMART
IQ	812	834	2.18e-2	SMART
IQ	835	857	1.72e0	SMART
IQ	860	882	7.52e-6	SMART
IQ	883	905	4.12e-3	SMART
low complexity region	1053	1065	N/A	INTRINSIC
coiled coil region	1140	1261	N/A	INTRINSIC
coiled coil region	1311	1415	N/A	INTRINSIC
DIL	1650	1755	7.48e-51	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000120161

Predicted Effect

probably damaging
Transcript: ENSMUST00000121875
AA Change: Y175C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000112728
Gene: ENSMUSG00000025885
AA Change: Y175C

Domain	Start	End	E-Value	Type
MYSc	63	763	N/A	SMART
IQ	764	786	2.41e-4	SMART
IQ	787	809	7.7e-3	SMART
IQ	812	834	2.18e-2	SMART
IQ	835	857	1.72e0	SMART
IQ	860	882	7.52e-6	SMART
IQ	883	905	4.12e-3	SMART
low complexity region	1053	1065	N/A	INTRINSIC
coiled coil region	1140	1261	N/A	INTRINSIC
coiled coil region	1332	1441	N/A	INTRINSIC
DIL	1676	1781	7.48e-51	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000125882
AA Change: Y173C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000123123
Gene: ENSMUSG00000025885
AA Change: Y173C

Domain	Start	End	E-Value	Type
MYSc	61	183	8.63e-4	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene, together with other proteins, may be involved in plasma membrane recycling. Mutations in this gene are associated with microvillous inclusion disease. [provided by RefSeq, Sep 2009]
PHENOTYPE: Homozygous null mice show perinatal mortality, diarrhea, intestinal microvillus atrophy and the presence of microvillus inclusion bodies, resembling phenotype of Microvillus Inclusion Disease. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700014D04Rik	T	C	13: 59,742,683 (GRCm38)	D441G	probably benign	Het
4933427D14Rik	T	C	11: 72,195,754 (GRCm38)	K277R	probably damaging	Het
Aatf	T	C	11: 84,473,100 (GRCm38)	Y267C	probably benign	Het
Abi3bp	A	G	16: 56,594,497 (GRCm38)	T341A	probably damaging	Het
Acer2	T	C	4: 86,874,586 (GRCm38)	F33S	probably damaging	Het
Actr1b	A	G	1: 36,701,163 (GRCm38)	V299A	possibly damaging	Het
Ampd3	T	A	7: 110,791,180 (GRCm38)		probably null	Het
Atm	A	C	9: 53,444,000 (GRCm38)	I2898R	probably damaging	Het
Bpifb6	G	C	2: 153,906,892 (GRCm38)	K269N	possibly damaging	Het
Cacna1c	T	A	6: 118,652,714 (GRCm38)	T1249S	probably benign	Het
Cacna1c	T	C	6: 118,598,723 (GRCm38)	E1927G	possibly damaging	Het
Cars2	TCCCC	TCCC	8: 11,529,599 (GRCm38)		probably null	Het
Cbll1	A	G	12: 31,487,508 (GRCm38)	V415A	probably benign	Het
Cd300lf	C	T	11: 115,124,369 (GRCm38)	V132I	probably benign	Het
Chd2	T	C	7: 73,463,671 (GRCm38)	E1187G	probably damaging	Het
Cntrl	T	C	2: 35,129,793 (GRCm38)	L544P	probably damaging	Het
Cryga	A	C	1: 65,103,010 (GRCm38)	S75A	probably benign	Het
Dcbld1	T	A	10: 52,319,480 (GRCm38)	Y261*	probably null	Het
Ddx11	G	A	17: 66,150,729 (GRCm38)		probably null	Het
Dgke	C	T	11: 89,040,749 (GRCm38)	V560I	probably benign	Het
Dst	A	C	1: 34,228,672 (GRCm38)	D5065A	probably damaging	Het
Dusp16	C	A	6: 134,720,493 (GRCm38)		probably null	Het
Eif4enif1	T	A	11: 3,227,793 (GRCm38)	V395E	probably damaging	Het
Enox1	A	G	14: 77,577,764 (GRCm38)	T121A	probably damaging	Het
Enpp4	G	T	17: 44,102,480 (GRCm38)	N54K	probably benign	Het
Erc2	A	T	14: 28,080,155 (GRCm38)	K764M	probably damaging	Het
Ercc6	G	T	14: 32,526,403 (GRCm38)	E304*	probably null	Het
Fam117a	T	A	11: 95,364,145 (GRCm38)	C115S	possibly damaging	Het
Fcrl5	G	A	3: 87,448,324 (GRCm38)	G448E	probably damaging	Het
Galntl5	T	C	5: 25,186,165 (GRCm38)	S21P	probably benign	Het
Garnl3	T	C	2: 33,104,880 (GRCm38)	D39G	probably benign	Het
Gm14295	C	T	2: 176,808,989 (GRCm38)	Q91*	probably null	Het
Grb10	T	A	11: 11,970,639 (GRCm38)		probably benign	Het
Grip1	C	T	10: 120,075,464 (GRCm38)	Q696*	probably null	Het
Herc2	T	G	7: 56,204,718 (GRCm38)	L3797R	possibly damaging	Het
Herc2	T	A	7: 56,153,166 (GRCm38)	C2112*	probably null	Het
Ighm	T	C	12: 113,421,567 (GRCm38)	I258V	unknown	Het
Jarid2	T	A	13: 44,903,063 (GRCm38)	Y443N	possibly damaging	Het
Kif13b	A	G	14: 64,738,634 (GRCm38)	Y466C	probably damaging	Het
Krtap4-6	T	A	11: 99,665,419 (GRCm38)	R161*	probably null	Het
Lingo4	G	A	3: 94,403,390 (GRCm38)	G545E	probably benign	Het
Lmo2	T	G	2: 103,970,601 (GRCm38)	V39G	possibly damaging	Het
Lor	C	A	3: 92,081,812 (GRCm38)	G56*	probably null	Het
Lrfn1	A	G	7: 28,459,744 (GRCm38)	R363G	probably benign	Het
Lrp1b	T	C	2: 40,657,525 (GRCm38)	D446G	probably benign	Het
Ltbp1	G	T	17: 75,005,989 (GRCm38)	G35V	possibly damaging	Het
Magel2	G	A	7: 62,378,679 (GRCm38)	V444M	probably damaging	Het
Mkx	A	T	18: 7,000,591 (GRCm38)		probably null	Het
Ms4a7	A	T	19: 11,333,295 (GRCm38)	I20N	possibly damaging	Het
Nek1	C	T	8: 61,072,309 (GRCm38)	Q594*	probably null	Het
Nipbl	T	C	15: 8,300,895 (GRCm38)	M2349V	possibly damaging	Het
Nmnat2	A	T	1: 153,076,971 (GRCm38)	H102L	probably damaging	Het
Nup155	T	A	15: 8,153,155 (GRCm38)	C1201S	probably damaging	Het
Olfr1249	G	A	2: 89,630,631 (GRCm38)	T89I	probably damaging	Het
Olfr1373	C	A	11: 52,144,596 (GRCm38)	R311S	probably benign	Het
Olfr686	G	A	7: 105,203,392 (GRCm38)	T317I	probably damaging	Het
Osbpl1a	A	G	18: 12,819,503 (GRCm38)		probably null	Het
Pcnt	A	G	10: 76,385,798 (GRCm38)	V1998A	probably benign	Het
Pitpnc1	T	C	11: 107,226,266 (GRCm38)	H193R	probably damaging	Het
Pitpnm1	T	C	19: 4,110,522 (GRCm38)	L781P	probably damaging	Het
Prdm14	A	T	1: 13,118,936 (GRCm38)	C395S	probably damaging	Het
Prmt8	A	G	6: 127,711,804 (GRCm38)	I201T	probably damaging	Het
Pter	T	C	2: 12,978,541 (GRCm38)	V119A	probably damaging	Het
Rab11fip4	T	C	11: 79,690,829 (GRCm38)		probably null	Het
Rgs9	T	C	11: 109,268,987 (GRCm38)	N173S	probably benign	Het
Rorb	C	A	19: 18,977,857 (GRCm38)	V47L	possibly damaging	Het
Rtn4r	A	G	16: 18,151,182 (GRCm38)	Y158C	probably damaging	Het
Sdr16c5	C	T	4: 4,016,162 (GRCm38)	G88E	probably damaging	Het
Sfxn1	C	T	13: 54,093,880 (GRCm38)	T208I	probably benign	Het
Sgo2b	C	T	8: 63,927,793 (GRCm38)	M668I	probably benign	Het
Slc52a3	G	T	2: 152,007,609 (GRCm38)		probably null	Het
Smco1	A	T	16: 32,274,014 (GRCm38)	M168L	probably benign	Het
Spata31d1d	G	A	13: 59,728,464 (GRCm38)	T419I	possibly damaging	Het
Spink5	T	C	18: 44,014,757 (GRCm38)	S857P	probably damaging	Het
Stk35	T	A	2: 129,810,888 (GRCm38)	Y436*	probably null	Het
Tmem225	T	A	9: 40,148,435 (GRCm38)	I37N	probably damaging	Het
Unkl	T	C	17: 25,231,865 (GRCm38)	*232R	probably null	Het
Usp16	A	G	16: 87,483,191 (GRCm38)	N813S	probably benign	Het
Vmn1r128	A	T	7: 21,350,296 (GRCm38)	*308C	probably null	Het
Vmn2r45	A	G	7: 8,472,208 (GRCm38)	V607A	probably benign	Het
Vmn2r63	A	T	7: 42,928,635 (GRCm38)		probably null	Het
Wnk4	A	T	11: 101,273,998 (GRCm38)	N718Y	probably damaging	Het
Zfp503	G	C	14: 21,985,800 (GRCm38)	Y349*	probably null	Het
Zfp990	T	A	4: 145,538,103 (GRCm38)	F557Y	possibly damaging	Het

Other mutations in Myo5b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00798:Myo5b	APN	18	74,654,076 (GRCm38)	splice site	probably benign
IGL01083:Myo5b	APN	18	74,733,903 (GRCm38)	splice site	probably benign
IGL01448:Myo5b	APN	18	74,644,090 (GRCm38)	missense	probably damaging	0.97
IGL01516:Myo5b	APN	18	74,627,195 (GRCm38)	missense	probably damaging	0.99
IGL01525:Myo5b	APN	18	74,740,549 (GRCm38)	missense	probably damaging	1.00
IGL01873:Myo5b	APN	18	74,580,396 (GRCm38)	missense	probably damaging	1.00
IGL01887:Myo5b	APN	18	74,714,936 (GRCm38)	missense	probably benign	0.41
IGL01953:Myo5b	APN	18	74,569,767 (GRCm38)	missense	possibly damaging	0.62
IGL01976:Myo5b	APN	18	74,698,277 (GRCm38)	missense	probably damaging	1.00
IGL02017:Myo5b	APN	18	74,716,999 (GRCm38)	missense	probably damaging	1.00
IGL02331:Myo5b	APN	18	74,638,040 (GRCm38)	critical splice acceptor site	probably null
IGL02624:Myo5b	APN	18	74,714,939 (GRCm38)	missense	probably damaging	0.98
IGL02707:Myo5b	APN	18	74,695,367 (GRCm38)	splice site	probably benign
IGL02806:Myo5b	APN	18	74,617,080 (GRCm38)	critical splice donor site	probably null
IGL03009:Myo5b	APN	18	74,760,968 (GRCm38)	missense	possibly damaging	0.54
IGL03061:Myo5b	APN	18	74,580,544 (GRCm38)	splice site	probably benign
IGL03061:Myo5b	APN	18	74,634,559 (GRCm38)	missense	probably benign	0.02
unrat	UTSW	18	74,653,361 (GRCm38)	missense	possibly damaging	0.93
BB007:Myo5b	UTSW	18	74,731,754 (GRCm38)	missense	probably benign
BB017:Myo5b	UTSW	18	74,731,754 (GRCm38)	missense	probably benign
R0085:Myo5b	UTSW	18	74,701,680 (GRCm38)	missense	probably benign	0.21
R0114:Myo5b	UTSW	18	74,742,171 (GRCm38)	missense	probably benign	0.03
R0226:Myo5b	UTSW	18	74,742,180 (GRCm38)	missense	probably benign
R0242:Myo5b	UTSW	18	74,661,716 (GRCm38)	missense	possibly damaging	0.95
R0242:Myo5b	UTSW	18	74,661,716 (GRCm38)	missense	possibly damaging	0.95
R0471:Myo5b	UTSW	18	74,728,954 (GRCm38)	splice site	probably benign
R0494:Myo5b	UTSW	18	74,653,967 (GRCm38)	missense	probably damaging	1.00
R0920:Myo5b	UTSW	18	74,625,641 (GRCm38)	missense	probably benign	0.09
R1144:Myo5b	UTSW	18	74,625,587 (GRCm38)	missense	probably damaging	1.00
R1177:Myo5b	UTSW	18	74,644,072 (GRCm38)	missense	probably damaging	1.00
R1387:Myo5b	UTSW	18	74,644,201 (GRCm38)	splice site	probably benign
R1468:Myo5b	UTSW	18	74,740,503 (GRCm38)	missense	probably damaging	0.99
R1468:Myo5b	UTSW	18	74,740,503 (GRCm38)	missense	probably damaging	0.99
R1555:Myo5b	UTSW	18	74,569,782 (GRCm38)	missense	probably damaging	1.00
R1587:Myo5b	UTSW	18	74,733,990 (GRCm38)	missense	probably benign
R1600:Myo5b	UTSW	18	74,713,540 (GRCm38)	unclassified	probably benign
R1639:Myo5b	UTSW	18	74,707,916 (GRCm38)	missense	probably benign	0.19
R1779:Myo5b	UTSW	18	74,742,147 (GRCm38)	missense	probably benign	0.06
R1806:Myo5b	UTSW	18	74,577,609 (GRCm38)	missense	possibly damaging	0.91
R1929:Myo5b	UTSW	18	74,733,925 (GRCm38)	missense	probably damaging	0.99
R2046:Myo5b	UTSW	18	74,577,455 (GRCm38)	missense	probably benign	0.28
R2093:Myo5b	UTSW	18	74,759,192 (GRCm38)	missense	probably damaging	0.98
R2270:Myo5b	UTSW	18	74,733,925 (GRCm38)	missense	probably damaging	0.99
R2272:Myo5b	UTSW	18	74,733,925 (GRCm38)	missense	probably damaging	0.99
R2298:Myo5b	UTSW	18	74,625,605 (GRCm38)	missense	probably damaging	1.00
R2433:Myo5b	UTSW	18	74,759,087 (GRCm38)	missense	probably damaging	1.00
R2888:Myo5b	UTSW	18	74,762,618 (GRCm38)	missense	probably damaging	1.00
R3824:Myo5b	UTSW	18	74,661,655 (GRCm38)	missense	probably benign	0.41
R3937:Myo5b	UTSW	18	74,716,037 (GRCm38)	missense	probably damaging	0.98
R3938:Myo5b	UTSW	18	74,716,037 (GRCm38)	missense	probably damaging	0.98
R3947:Myo5b	UTSW	18	74,695,403 (GRCm38)	missense	probably damaging	1.00
R3971:Myo5b	UTSW	18	74,740,527 (GRCm38)	missense	probably damaging	1.00
R3972:Myo5b	UTSW	18	74,740,527 (GRCm38)	missense	probably damaging	1.00
R3974:Myo5b	UTSW	18	74,634,481 (GRCm38)	missense	probably damaging	1.00
R4027:Myo5b	UTSW	18	74,759,240 (GRCm38)	missense	possibly damaging	0.67
R4080:Myo5b	UTSW	18	74,740,488 (GRCm38)	missense	probably benign
R4285:Myo5b	UTSW	18	74,714,849 (GRCm38)	missense	probably benign
R4308:Myo5b	UTSW	18	74,731,740 (GRCm38)	missense	possibly damaging	0.89
R4411:Myo5b	UTSW	18	74,698,274 (GRCm38)	missense	possibly damaging	0.89
R4415:Myo5b	UTSW	18	74,580,408 (GRCm38)	missense	probably damaging	1.00
R4516:Myo5b	UTSW	18	74,625,674 (GRCm38)	missense	probably damaging	1.00
R4690:Myo5b	UTSW	18	74,722,462 (GRCm38)	missense	probably damaging	0.97
R4781:Myo5b	UTSW	18	74,744,681 (GRCm38)	missense	possibly damaging	0.80
R4786:Myo5b	UTSW	18	74,695,380 (GRCm38)	missense	probably benign	0.01
R4796:Myo5b	UTSW	18	74,744,630 (GRCm38)	missense	possibly damaging	0.68
R4924:Myo5b	UTSW	18	74,695,384 (GRCm38)	missense	probably benign	0.19
R4972:Myo5b	UTSW	18	74,627,193 (GRCm38)	missense	probably damaging	0.98
R5004:Myo5b	UTSW	18	74,744,773 (GRCm38)	critical splice donor site	probably null
R5024:Myo5b	UTSW	18	74,716,034 (GRCm38)	missense	possibly damaging	0.90
R5043:Myo5b	UTSW	18	74,638,153 (GRCm38)	critical splice donor site	probably null
R5187:Myo5b	UTSW	18	74,701,674 (GRCm38)	missense	possibly damaging	0.68
R5232:Myo5b	UTSW	18	74,714,932 (GRCm38)	missense	probably damaging	0.99
R5254:Myo5b	UTSW	18	74,700,606 (GRCm38)	missense	possibly damaging	0.65
R5255:Myo5b	UTSW	18	74,662,670 (GRCm38)	missense	possibly damaging	0.94
R5715:Myo5b	UTSW	18	74,742,175 (GRCm38)	missense	possibly damaging	0.88
R5733:Myo5b	UTSW	18	74,654,057 (GRCm38)	missense	possibly damaging	0.93
R5797:Myo5b	UTSW	18	74,701,521 (GRCm38)	missense	probably benign
R5875:Myo5b	UTSW	18	74,707,902 (GRCm38)	splice site	probably null
R6088:Myo5b	UTSW	18	74,720,898 (GRCm38)	missense	possibly damaging	0.89
R6104:Myo5b	UTSW	18	74,700,679 (GRCm38)	missense	probably benign	0.19
R6237:Myo5b	UTSW	18	74,742,178 (GRCm38)	missense	probably damaging	1.00
R6265:Myo5b	UTSW	18	74,577,440 (GRCm38)	splice site	probably null
R6328:Myo5b	UTSW	18	74,616,993 (GRCm38)	missense	probably damaging	1.00
R6330:Myo5b	UTSW	18	74,616,993 (GRCm38)	missense	probably damaging	1.00
R6331:Myo5b	UTSW	18	74,616,993 (GRCm38)	missense	probably damaging	1.00
R6347:Myo5b	UTSW	18	74,770,385 (GRCm38)	missense	probably benign	0.11
R6479:Myo5b	UTSW	18	74,617,015 (GRCm38)	missense	probably damaging	1.00
R6748:Myo5b	UTSW	18	74,701,503 (GRCm38)	missense	possibly damaging	0.80
R6749:Myo5b	UTSW	18	74,701,503 (GRCm38)	missense	possibly damaging	0.80
R6750:Myo5b	UTSW	18	74,617,035 (GRCm38)	missense	possibly damaging	0.74
R6833:Myo5b	UTSW	18	74,770,325 (GRCm38)	missense	probably benign
R6876:Myo5b	UTSW	18	74,707,955 (GRCm38)	missense	probably benign
R6880:Myo5b	UTSW	18	74,722,430 (GRCm38)	missense	probably benign	0.02
R6902:Myo5b	UTSW	18	74,676,685 (GRCm38)	missense	possibly damaging	0.95
R6985:Myo5b	UTSW	18	74,653,361 (GRCm38)	missense	possibly damaging	0.93
R7039:Myo5b	UTSW	18	74,701,528 (GRCm38)	missense	probably benign	0.01
R7162:Myo5b	UTSW	18	74,695,427 (GRCm38)	missense	probably benign	0.02
R7345:Myo5b	UTSW	18	74,708,024 (GRCm38)	missense	possibly damaging	0.82
R7530:Myo5b	UTSW	18	74,731,731 (GRCm38)	missense	probably benign	0.00
R7564:Myo5b	UTSW	18	74,634,511 (GRCm38)	missense	possibly damaging	0.84
R7629:Myo5b	UTSW	18	74,627,254 (GRCm38)	critical splice donor site	probably null
R7635:Myo5b	UTSW	18	74,580,396 (GRCm38)	missense	probably damaging	1.00
R7670:Myo5b	UTSW	18	74,701,446 (GRCm38)	missense	probably benign	0.05
R7754:Myo5b	UTSW	18	74,634,559 (GRCm38)	missense	probably benign	0.02
R7930:Myo5b	UTSW	18	74,731,754 (GRCm38)	missense	probably benign
R8013:Myo5b	UTSW	18	74,760,899 (GRCm38)	nonsense	probably null
R8271:Myo5b	UTSW	18	74,627,190 (GRCm38)	missense	probably damaging	1.00
R8312:Myo5b	UTSW	18	74,733,962 (GRCm38)	missense	probably damaging	1.00
R8383:Myo5b	UTSW	18	74,643,978 (GRCm38)	missense	probably benign	0.05
R8384:Myo5b	UTSW	18	74,742,202 (GRCm38)	missense	probably damaging	1.00
R8474:Myo5b	UTSW	18	74,770,340 (GRCm38)	missense	probably damaging	1.00
R8825:Myo5b	UTSW	18	74,759,098 (GRCm38)	missense	possibly damaging	0.79
R8846:Myo5b	UTSW	18	74,707,972 (GRCm38)	missense	probably benign	0.04
R9236:Myo5b	UTSW	18	74,720,863 (GRCm38)	missense	probably benign
R9283:Myo5b	UTSW	18	74,644,078 (GRCm38)	missense	probably benign	0.16
R9370:Myo5b	UTSW	18	74,627,175 (GRCm38)	missense	possibly damaging	0.54
R9506:Myo5b	UTSW	18	74,744,760 (GRCm38)	missense	possibly damaging	0.82
R9523:Myo5b	UTSW	18	74,728,897 (GRCm38)	missense	possibly damaging	0.89
R9622:Myo5b	UTSW	18	74,714,946 (GRCm38)	missense	probably damaging	0.99
R9676:Myo5b	UTSW	18	74,759,160 (GRCm38)	missense	probably benign	0.22
R9725:Myo5b	UTSW	18	74,723,770 (GRCm38)	missense	probably benign
RF009:Myo5b	UTSW	18	74,643,999 (GRCm38)	missense	probably damaging	1.00
Z1088:Myo5b	UTSW	18	74,744,749 (GRCm38)	missense	probably benign	0.35
Z1177:Myo5b	UTSW	18	74,617,017 (GRCm38)	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- CCACATTTATTCCCCAAGAGATGC -3'
(R):5'- TTTCAGAGGTACCTCCACCC -3'

Sequencing Primer
(F):5'- GAGATGCTTCTCACCAGGTTAC -3'
(R):5'- AATGCCAGGGCTGCCTAAG -3'

Posted On

2018-03-15