Mutagenetix > Incidental Mutation

Incidental Mutation 'R6276:Map2'

507613

Institutional Source

Beutler Lab

Gene Symbol

Map2

Ensembl Gene

ENSMUSG00000015222

Gene Name

microtubule-associated protein 2

Synonyms

repro4, MAP-2, Mtap2, G1-397-34

MMRRC Submission

044446-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.775) question?

Stock #

R6276 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66175273-66442583 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 66399419 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 34 (V34A)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024639] [ENSMUST00000077355] [ENSMUST00000114012] [ENSMUST00000114013] [ENSMUST00000114015] [ENSMUST00000114017] [ENSMUST00000114018] [ENSMUST00000123647] [ENSMUST00000145419] [ENSMUST00000173855]

AlphaFold

P20357

Predicted Effect

probably damaging
Transcript: ENSMUST00000024639
AA Change: V98A

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000024639
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000077355
AA Change: V98A

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000076577
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114012
AA Change: V98A

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000109645
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
low complexity region	133	140	N/A	INTRINSIC
low complexity region	181	195	N/A	INTRINSIC
low complexity region	205	221	N/A	INTRINSIC
low complexity region	228	250	N/A	INTRINSIC
Pfam:Tubulin-binding	299	330	2.1e-18	PFAM
Pfam:Tubulin-binding	331	361	9.1e-20	PFAM
Pfam:Tubulin-binding	362	393	1.7e-17	PFAM
low complexity region	421	434	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114013
AA Change: V98A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000109646
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	86	103	1.2e-5	PFAM
low complexity region	120	141	N/A	INTRINSIC
Pfam:MAP2_projctn	376	1510	N/A	PFAM
low complexity region	1543	1557	N/A	INTRINSIC
low complexity region	1567	1583	N/A	INTRINSIC
low complexity region	1590	1612	N/A	INTRINSIC
Pfam:Tubulin-binding	1662	1692	1.7e-13	PFAM
Pfam:Tubulin-binding	1693	1723	5.8e-18	PFAM
Pfam:Tubulin-binding	1724	1755	5.9e-18	PFAM
low complexity region	1783	1796	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114015
AA Change: V98A

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000109648
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114017
AA Change: V98A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000109650
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114018
AA Change: V98A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000109651
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000123647
AA Change: V98A

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000119341
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	86	103	3.8e-5	PFAM
low complexity region	120	135	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127388

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128673

Predicted Effect

probably damaging
Transcript: ENSMUST00000141148
AA Change: V34A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000117996
Gene: ENSMUSG00000015222
AA Change: V34A

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	23	40	3.2e-6	PFAM
low complexity region	70	77	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000145419

SMART Domains

Protein: ENSMUSP00000134538
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	218	608	1.7e-250	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151423

Predicted Effect

possibly damaging
Transcript: ENSMUST00000173855
AA Change: V98A

PolyPhen 2 Score 0.888 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000134471
Gene: ENSMUSG00000015222
AA Change: V98A

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	142	163	N/A	INTRINSIC
Pfam:MAP2_projctn	458	565	1.1e-52	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174590

Meta Mutation Damage Score

0.0742

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.3%
20x: 95.5%

Validation Efficiency

100% (71/71)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit altered contextual memory. Mice homozygous for another knock-out allele display decreased body weight, altered microtubule density and organization in Purkinje cell dendrites, and reduced dendritic length inhippocampal neurons. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr3	A	T	1: 125,395,137 (GRCm38)	D364E	probably benign	Het
Adra2c	C	T	5: 35,280,079 (GRCm38)	T65I	probably damaging	Het
Agap2	T	A	10: 127,089,360 (GRCm38)		probably null	Het
Ager	T	C	17: 34,598,754 (GRCm38)	V126A	possibly damaging	Het
Arhgap39	T	C	15: 76,737,536 (GRCm38)	I288M	probably benign	Het
Arhgap45	A	T	10: 80,026,234 (GRCm38)	S541C	probably benign	Het
Asb4	A	G	6: 5,431,043 (GRCm38)	Y426C	probably damaging	Het
Azi2	C	T	9: 118,049,338 (GRCm38)	T82I	probably damaging	Het
Baz2b	T	A	2: 59,948,223 (GRCm38)	R764S	probably damaging	Het
Ccdc146	A	C	5: 21,301,340 (GRCm38)	I701S	probably damaging	Het
Ccnq	T	C	11: 78,751,230 (GRCm38)	K145E	probably damaging	Het
Cd55b	T	A	1: 130,418,166 (GRCm38)	I172F	probably damaging	Het
Cdk11b	A	G	4: 155,634,190 (GRCm38)	E199G	probably benign	Het
Cntnap4	A	T	8: 112,752,289 (GRCm38)	T216S	possibly damaging	Het
D5Ertd579e	A	T	5: 36,604,514 (GRCm38)	N1336K	possibly damaging	Het
Dlg5	T	C	14: 24,164,568 (GRCm38)	N649S	probably damaging	Het
Dmxl1	A	G	18: 49,846,586 (GRCm38)	E96G	probably benign	Het
Dscaml1	C	T	9: 45,668,160 (GRCm38)	T335I	possibly damaging	Het
Epb41l2	G	T	10: 25,502,124 (GRCm38)	G695C	probably damaging	Het
Erbb4	C	T	1: 68,560,576 (GRCm38)	R114H	probably damaging	Het
F2rl1	G	T	13: 95,513,938 (GRCm38)	Y145*	probably null	Het
Fsip2	A	T	2: 82,980,441 (GRCm38)	Y2368F	possibly damaging	Het
Galnt7	C	T	8: 57,536,578 (GRCm38)		probably null	Het
Garre1	A	T	7: 34,242,377 (GRCm38)	Y627*	probably null	Het
Gm6811	T	G	17: 21,094,690 (GRCm38)		noncoding transcript	Het
Gm6811	T	A	17: 21,093,983 (GRCm38)		noncoding transcript	Het
H2-M1	G	A	17: 36,671,710 (GRCm38)	T86M	possibly damaging	Het
Hk2	T	C	6: 82,743,366 (GRCm38)	D170G	probably benign	Het
Hmcn1	C	T	1: 150,738,681 (GRCm38)	A1325T	possibly damaging	Het
Insrr	T	A	3: 87,800,519 (GRCm38)	Y89*	probably null	Het
Itga1	T	C	13: 114,980,852 (GRCm38)	E871G	probably benign	Het
Kat6a	T	C	8: 22,939,405 (GRCm38)	L1592P	possibly damaging	Het
Kndc1	C	A	7: 139,921,063 (GRCm38)	A756E	probably benign	Het
Krt12	A	T	11: 99,421,902 (GRCm38)	C105*	probably null	Het
Lama1	G	A	17: 67,784,088 (GRCm38)		probably null	Het
Lama3	A	G	18: 12,506,949 (GRCm38)	N67S	probably benign	Het
Lrrk1	A	G	7: 66,306,839 (GRCm38)		probably null	Het
Lypd9	T	A	11: 58,446,366 (GRCm38)	I94L	possibly damaging	Het
Mroh6	A	G	15: 75,885,700 (GRCm38)	L487P	probably damaging	Het
Myo18b	A	T	5: 112,811,642 (GRCm38)	S1430T	probably benign	Het
Notch3	G	A	17: 32,154,749 (GRCm38)	T495I	probably benign	Het
Olfr391-ps	A	T	11: 73,799,403 (GRCm38)	M118K	probably damaging	Het
Palld	C	T	8: 61,513,423 (GRCm38)	A980T	probably damaging	Het
Paxip1	A	T	5: 27,761,668 (GRCm38)	I620N	probably damaging	Het
Pcdha6	A	G	18: 36,969,767 (GRCm38)		probably null	Het
Pcdhb11	G	A	18: 37,421,760 (GRCm38)	V48M	probably benign	Het
Pcdhga6	A	G	18: 37,707,644 (GRCm38)	E139G	probably benign	Het
Pck1	T	C	2: 173,157,319 (GRCm38)	V426A	probably damaging	Het
Pck2	T	A	14: 55,542,624 (GRCm38)	I110N	probably damaging	Het
Peg10	GAT	GATCAT	6: 4,756,449 (GRCm38)		probably benign	Het
Phactr3	G	A	2: 178,279,019 (GRCm38)	E222K	probably damaging	Het
Ppip5k1	A	T	2: 121,323,203 (GRCm38)		probably benign	Het
Prodh2	A	G	7: 30,506,651 (GRCm38)	H278R	probably benign	Het
Rspry1	T	A	8: 94,623,258 (GRCm38)	H91Q	probably damaging	Het
Slc13a2	CGTTATCTGT	CGT	11: 78,403,480 (GRCm38)		probably benign	Het
Smn1	A	G	13: 100,127,995 (GRCm38)	N78S	possibly damaging	Het
Spta1	T	A	1: 174,218,512 (GRCm38)	I1614N	probably damaging	Het
Syt17	T	C	7: 118,434,290 (GRCm38)	D165G	probably damaging	Het
Tbck	A	G	3: 132,743,005 (GRCm38)	Y593C	probably damaging	Het
Tcaf2	G	C	6: 42,629,753 (GRCm38)	F422L	probably benign	Het
Tex15	T	A	8: 33,577,189 (GRCm38)	F2216I	possibly damaging	Het
Trpc4	A	G	3: 54,318,020 (GRCm38)	E846G	probably benign	Het
Ttc41	T	C	10: 86,744,449 (GRCm38)	I753T	probably benign	Het
Vdac1	C	T	11: 52,376,482 (GRCm38)	T70M	possibly damaging	Het
Vmn1r124	A	C	7: 21,260,179 (GRCm38)	F147V	probably benign	Het
Vmn1r220	T	C	13: 23,184,295 (GRCm38)	D77G	probably damaging	Het
Vmn2r28	T	C	7: 5,490,731 (GRCm38)	H72R	probably benign	Het
Vmn2r78	A	T	7: 86,921,110 (GRCm38)	I279L	probably benign	Het
Vmn2r95	G	T	17: 18,451,470 (GRCm38)	A490S	possibly damaging	Het
Wdfy4	C	T	14: 33,109,525 (GRCm38)	A915T	possibly damaging	Het
Zmiz2	G	T	11: 6,395,604 (GRCm38)		probably null	Het
Zscan2	A	G	7: 80,875,809 (GRCm38)	N426S	probably benign	Het

Other mutations in Map2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Map2	APN	1	66,425,331 (GRCm38)	missense	probably damaging	1.00
IGL02135:Map2	APN	1	66,380,761 (GRCm38)	nonsense	probably null
IGL02526:Map2	APN	1	66,380,717 (GRCm38)	missense	possibly damaging	0.94
Annas	UTSW	1	66,433,597 (GRCm38)	critical splice donor site	probably null
calliope	UTSW	1	66,425,298 (GRCm38)	missense	probably damaging	1.00
carthage	UTSW	1	66,414,648 (GRCm38)	missense	probably damaging	1.00
costas	UTSW	1	66,412,653 (GRCm38)	missense	possibly damaging	0.94
Jacobin	UTSW	1	66,399,419 (GRCm38)	missense	probably damaging	1.00
Nectar	UTSW	1	66,415,363 (GRCm38)	nonsense	probably null
ruby_throat	UTSW	1	66,414,884 (GRCm38)	missense	possibly damaging	0.67
Rufous	UTSW	1	66,380,768 (GRCm38)	missense	probably damaging	1.00
Speckled	UTSW	1	66,412,727 (GRCm38)	nonsense	probably null
Sunbird	UTSW	1	66,415,487 (GRCm38)	missense	probably benign	0.01
swift	UTSW	1	66,416,364 (GRCm38)	missense	probably damaging	1.00
E0370:Map2	UTSW	1	66,416,724 (GRCm38)	unclassified	probably benign
PIT4362001:Map2	UTSW	1	66,412,518 (GRCm38)	missense	probably benign	0.05
R0067:Map2	UTSW	1	66,413,163 (GRCm38)	missense	probably benign	0.04
R0238:Map2	UTSW	1	66,416,106 (GRCm38)	missense	probably damaging	1.00
R0238:Map2	UTSW	1	66,416,106 (GRCm38)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,416,106 (GRCm38)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,416,106 (GRCm38)	missense	probably damaging	1.00
R0268:Map2	UTSW	1	66,380,722 (GRCm38)	nonsense	probably null
R0302:Map2	UTSW	1	66,414,828 (GRCm38)	missense	probably benign	0.15
R0305:Map2	UTSW	1	66,413,094 (GRCm38)	missense	probably benign	0.00
R0409:Map2	UTSW	1	66,433,580 (GRCm38)	missense	probably damaging	1.00
R0561:Map2	UTSW	1	66,425,497 (GRCm38)	missense	probably damaging	1.00
R0674:Map2	UTSW	1	66,413,202 (GRCm38)	missense	probably damaging	1.00
R0738:Map2	UTSW	1	66,425,189 (GRCm38)	splice site	probably benign
R0893:Map2	UTSW	1	66,380,768 (GRCm38)	missense	probably damaging	1.00
R1305:Map2	UTSW	1	66,425,395 (GRCm38)	missense	probably damaging	1.00
R1534:Map2	UTSW	1	66,413,180 (GRCm38)	missense	probably benign	0.33
R1632:Map2	UTSW	1	66,415,086 (GRCm38)	missense	possibly damaging	0.60
R1682:Map2	UTSW	1	66,415,622 (GRCm38)	splice site	probably null
R1774:Map2	UTSW	1	66,414,074 (GRCm38)	missense	probably damaging	1.00
R2014:Map2	UTSW	1	66,416,136 (GRCm38)	missense	possibly damaging	0.55
R2017:Map2	UTSW	1	66,412,799 (GRCm38)	missense	probably damaging	1.00
R2050:Map2	UTSW	1	66,414,314 (GRCm38)	missense	probably damaging	0.98
R2093:Map2	UTSW	1	66,399,440 (GRCm38)	missense	probably damaging	1.00
R2214:Map2	UTSW	1	66,420,186 (GRCm38)	missense	probably damaging	0.99
R2284:Map2	UTSW	1	66,414,068 (GRCm38)	missense	probably damaging	1.00
R3011:Map2	UTSW	1	66,414,612 (GRCm38)	missense	probably damaging	1.00
R3105:Map2	UTSW	1	66,433,597 (GRCm38)	critical splice donor site	probably null
R3708:Map2	UTSW	1	66,416,555 (GRCm38)	unclassified	probably benign
R3709:Map2	UTSW	1	66,415,856 (GRCm38)	nonsense	probably null
R3729:Map2	UTSW	1	66,412,446 (GRCm38)	missense	possibly damaging	0.80
R3760:Map2	UTSW	1	66,438,918 (GRCm38)	missense	probably damaging	1.00
R3788:Map2	UTSW	1	66,416,863 (GRCm38)	missense	probably damaging	0.99
R3789:Map2	UTSW	1	66,416,863 (GRCm38)	missense	probably damaging	0.99
R4003:Map2	UTSW	1	66,415,740 (GRCm38)	missense	probably damaging	1.00
R4120:Map2	UTSW	1	66,415,904 (GRCm38)	missense	probably damaging	1.00
R4172:Map2	UTSW	1	66,413,600 (GRCm38)	missense	possibly damaging	0.89
R4198:Map2	UTSW	1	66,425,298 (GRCm38)	missense	probably damaging	1.00
R4200:Map2	UTSW	1	66,425,298 (GRCm38)	missense	probably damaging	1.00
R4205:Map2	UTSW	1	66,425,290 (GRCm38)	missense	probably damaging	1.00
R4613:Map2	UTSW	1	66,425,469 (GRCm38)	missense	probably damaging	1.00
R4700:Map2	UTSW	1	66,410,637 (GRCm38)	missense	probably damaging	0.96
R4974:Map2	UTSW	1	66,413,505 (GRCm38)	missense	probably benign	0.15
R5007:Map2	UTSW	1	66,413,289 (GRCm38)	missense	possibly damaging	0.86
R5039:Map2	UTSW	1	66,438,796 (GRCm38)	missense	probably damaging	1.00
R5237:Map2	UTSW	1	66,439,010 (GRCm38)	unclassified	probably benign
R5313:Map2	UTSW	1	66,425,379 (GRCm38)	missense	probably damaging	1.00
R5455:Map2	UTSW	1	66,399,391 (GRCm38)	missense	probably damaging	1.00
R5490:Map2	UTSW	1	66,413,133 (GRCm38)	missense	probably damaging	1.00
R5517:Map2	UTSW	1	66,415,256 (GRCm38)	missense	probably benign	0.00
R5532:Map2	UTSW	1	66,414,620 (GRCm38)	missense	probably damaging	1.00
R5583:Map2	UTSW	1	66,416,037 (GRCm38)	missense	probably damaging	1.00
R5764:Map2	UTSW	1	66,414,875 (GRCm38)	missense	probably damaging	0.99
R5996:Map2	UTSW	1	66,414,884 (GRCm38)	missense	possibly damaging	0.67
R6058:Map2	UTSW	1	66,415,414 (GRCm38)	missense	probably benign	0.05
R6199:Map2	UTSW	1	66,425,478 (GRCm38)	missense	probably damaging	1.00
R6208:Map2	UTSW	1	66,431,590 (GRCm38)	missense	probably damaging	1.00
R6378:Map2	UTSW	1	66,415,329 (GRCm38)	missense	probably damaging	1.00
R6424:Map2	UTSW	1	66,414,787 (GRCm38)	missense	possibly damaging	0.67
R6743:Map2	UTSW	1	66,415,607 (GRCm38)	missense	probably benign	0.04
R6837:Map2	UTSW	1	66,414,572 (GRCm38)	missense	probably damaging	1.00
R6901:Map2	UTSW	1	66,421,773 (GRCm38)	missense	possibly damaging	0.94
R6984:Map2	UTSW	1	66,415,236 (GRCm38)	missense	possibly damaging	0.90
R6989:Map2	UTSW	1	66,414,906 (GRCm38)	missense	probably benign	0.00
R7001:Map2	UTSW	1	66,415,487 (GRCm38)	missense	probably benign	0.01
R7055:Map2	UTSW	1	66,416,824 (GRCm38)	missense	probably damaging	1.00
R7094:Map2	UTSW	1	66,412,727 (GRCm38)	nonsense	probably null
R7106:Map2	UTSW	1	66,410,744 (GRCm38)	missense	possibly damaging	0.92
R7182:Map2	UTSW	1	66,412,653 (GRCm38)	missense	possibly damaging	0.94
R7235:Map2	UTSW	1	66,414,648 (GRCm38)	missense	probably damaging	1.00
R7424:Map2	UTSW	1	66,414,824 (GRCm38)	missense	possibly damaging	0.69
R7473:Map2	UTSW	1	66,415,458 (GRCm38)	missense	probably damaging	1.00
R7642:Map2	UTSW	1	66,413,307 (GRCm38)	missense	probably benign	0.01
R7660:Map2	UTSW	1	66,414,377 (GRCm38)	missense	probably damaging	1.00
R7673:Map2	UTSW	1	66,413,774 (GRCm38)	missense	probably benign	0.03
R7768:Map2	UTSW	1	66,414,483 (GRCm38)	missense	possibly damaging	0.94
R7796:Map2	UTSW	1	66,416,495 (GRCm38)	splice site	probably null
R7834:Map2	UTSW	1	66,416,488 (GRCm38)	missense	probably damaging	1.00
R7842:Map2	UTSW	1	66,416,411 (GRCm38)	missense	probably benign
R7955:Map2	UTSW	1	66,413,716 (GRCm38)	missense	probably damaging	1.00
R8056:Map2	UTSW	1	66,415,620 (GRCm38)	missense	probably damaging	0.99
R8118:Map2	UTSW	1	66,425,391 (GRCm38)	missense	probably damaging	1.00
R8135:Map2	UTSW	1	66,413,669 (GRCm38)	missense	probably damaging	1.00
R8152:Map2	UTSW	1	66,414,743 (GRCm38)	missense	probably benign	0.00
R8223:Map2	UTSW	1	66,425,490 (GRCm38)	missense	probably damaging	1.00
R8329:Map2	UTSW	1	66,415,113 (GRCm38)	missense	probably benign	0.01
R8344:Map2	UTSW	1	66,421,713 (GRCm38)	missense	probably damaging	1.00
R8475:Map2	UTSW	1	66,414,005 (GRCm38)	missense	probably damaging	1.00
R8548:Map2	UTSW	1	66,413,340 (GRCm38)	missense	probably damaging	1.00
R8680:Map2	UTSW	1	66,421,713 (GRCm38)	missense	probably damaging	1.00
R8713:Map2	UTSW	1	66,414,622 (GRCm38)	missense	probably damaging	1.00
R8745:Map2	UTSW	1	66,413,397 (GRCm38)	missense	probably benign	0.00
R8786:Map2	UTSW	1	66,433,596 (GRCm38)	critical splice donor site	probably benign
R8790:Map2	UTSW	1	66,438,838 (GRCm38)	missense	probably damaging	1.00
R8874:Map2	UTSW	1	66,416,364 (GRCm38)	missense	probably damaging	1.00
R8887:Map2	UTSW	1	66,415,599 (GRCm38)	missense	possibly damaging	0.48
R8948:Map2	UTSW	1	66,380,525 (GRCm38)	missense	probably damaging	1.00
R8950:Map2	UTSW	1	66,380,525 (GRCm38)	missense	probably damaging	1.00
R8998:Map2	UTSW	1	66,413,314 (GRCm38)	missense	possibly damaging	0.91
R8999:Map2	UTSW	1	66,413,314 (GRCm38)	missense	possibly damaging	0.91
R9072:Map2	UTSW	1	66,414,153 (GRCm38)	missense	probably damaging	1.00
R9088:Map2	UTSW	1	66,414,614 (GRCm38)	missense	probably damaging	1.00
R9089:Map2	UTSW	1	66,412,939 (GRCm38)	missense	probably benign	0.09
R9106:Map2	UTSW	1	66,415,363 (GRCm38)	nonsense	probably null
R9112:Map2	UTSW	1	66,433,564 (GRCm38)	nonsense	probably null
R9120:Map2	UTSW	1	66,414,059 (GRCm38)	missense	probably damaging	1.00
R9161:Map2	UTSW	1	66,438,344 (GRCm38)	missense	possibly damaging	0.65
R9464:Map2	UTSW	1	66,415,338 (GRCm38)	missense	probably damaging	1.00
R9589:Map2	UTSW	1	66,410,594 (GRCm38)	missense	probably benign
V8831:Map2	UTSW	1	66,415,845 (GRCm38)	missense	probably damaging	1.00
Z1177:Map2	UTSW	1	66,438,361 (GRCm38)	missense	probably damaging	0.96
Z1177:Map2	UTSW	1	66,380,680 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GGGACCCTAGAAGAAAAGTTCTC -3'
(R):5'- TGCTTCTAGTAGAGGTGACAGG -3'

Sequencing Primer
(F):5'- GACCCTAGAAGAAAAGTTCTCAATTG -3'
(R):5'- CTTCTAGTAGAGGTGACAGGTGAATG -3'

Posted On

2018-03-15