Mutagenetix > Incidental Mutation

Incidental Mutation 'R6277:Lama4'

507723

Institutional Source

Beutler Lab

Gene Symbol

Lama4

Ensembl Gene

ENSMUSG00000019846

Gene Name

laminin, alpha 4

Synonyms

laminin [a]4

MMRRC Submission

044447-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6277 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38841511-38986184 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 38982006 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 1745 (N1745S)

Ref Sequence

ENSEMBL: ENSMUSP00000019992 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019992]

AlphaFold

P97927

Predicted Effect

probably damaging
Transcript: ENSMUST00000019992
AA Change: N1745S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000019992
Gene: ENSMUSG00000019846
AA Change: N1745S

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
EGF_Lam	82	129	1.95e-8	SMART
EGF_Lam	132	184	5.78e-11	SMART
EGF_Lam	187	238	9.83e-14	SMART
Pfam:Laminin_I	283	548	5.3e-71	PFAM
coiled coil region	658	685	N/A	INTRINSIC
LamG	850	1009	9.54e-11	SMART
LamG	1066	1205	5.9e-25	SMART
LamG	1250	1374	6.68e-24	SMART
LamG	1484	1619	1.54e-37	SMART
LamG	1661	1794	3.63e-34	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000163036

Meta Mutation Damage Score

0.1366

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.2%

Validation Efficiency

96% (70/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]
PHENOTYPE: Homozygotes for targeted null mutations exhibit impaired motor control of the hind limbs associated with improperly positioned synaptic active zones and junctional folds, and prenatal and neonatal hemorrhages associated with capillary defects. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	A	G	14: 32,385,651 (GRCm39)	Y105H	possibly damaging	Het
4921539E11Rik	A	T	4: 103,088,668 (GRCm39)	Y179*	probably null	Het
Abca7	G	C	10: 79,841,992 (GRCm39)	V1042L	probably benign	Het
Adck5	C	A	15: 76,477,463 (GRCm39)	T99K	possibly damaging	Het
Adcy5	A	G	16: 35,109,896 (GRCm39)	T823A	probably benign	Het
Adra1d	G	T	2: 131,403,083 (GRCm39)	R336S	probably damaging	Het
Arhgap39	A	T	15: 76,619,337 (GRCm39)	M749K	probably damaging	Het
Arid4a	A	G	12: 71,086,665 (GRCm39)	R168G	possibly damaging	Het
Atxn10	A	G	15: 85,275,893 (GRCm39)	T317A	probably benign	Het
Bicc1	T	C	10: 70,863,731 (GRCm39)	K22E	possibly damaging	Het
Cacna1b	T	G	2: 24,620,808 (GRCm39)	T281P	probably damaging	Het
Ccdc174	A	T	6: 91,857,272 (GRCm39)	Q26L	probably damaging	Het
Celf3	T	C	3: 94,392,672 (GRCm39)	C124R	probably damaging	Het
Cfap44	A	G	16: 44,257,669 (GRCm39)	E1068G	probably benign	Het
Crybg1	T	A	10: 43,873,255 (GRCm39)	E1284D	probably benign	Het
Dcbld2	T	A	16: 58,272,119 (GRCm39)	Y392N	probably damaging	Het
Dcbld2	C	T	16: 58,285,866 (GRCm39)	P675L	probably damaging	Het
Dnah12	A	T	14: 26,492,439 (GRCm39)	H1193L	probably damaging	Het
Efcab3	G	C	11: 104,901,148 (GRCm39)	E4422D	possibly damaging	Het
Ern2	A	G	7: 121,785,330 (GRCm39)	F16L	probably benign	Het
Fam163b	G	T	2: 27,002,763 (GRCm39)	T78N	probably benign	Het
Fbxw21	A	T	9: 108,974,623 (GRCm39)	I299K	possibly damaging	Het
Foxg1	A	G	12: 49,432,299 (GRCm39)	N344S	probably benign	Het
Git2	A	G	5: 114,871,308 (GRCm39)	I202T	probably damaging	Het
Gm11595	G	T	11: 99,663,510 (GRCm39)	P57T	unknown	Het
Gm12258	G	A	11: 58,745,113 (GRCm39)	V15M	probably damaging	Het
Gm15056	C	T	8: 21,390,914 (GRCm39)	G40S	probably damaging	Het
Gnal	C	A	18: 67,346,143 (GRCm39)	H274Q	probably damaging	Het
Hormad2	A	T	11: 4,371,583 (GRCm39)		probably null	Het
Ighv7-2	A	G	12: 113,876,087 (GRCm39)	I6T	probably benign	Het
Irf9	A	G	14: 55,845,109 (GRCm39)	D323G	probably benign	Het
Klhl23	G	T	2: 69,664,096 (GRCm39)	D482Y	probably damaging	Het
Lct	T	A	1: 128,231,974 (GRCm39)	Y625F	probably benign	Het
Lrrc26	T	C	2: 25,180,116 (GRCm39)	V39A	probably benign	Het
Mllt6	G	A	11: 97,564,774 (GRCm39)	A527T	probably damaging	Het
Mrgprb4	T	A	7: 47,848,649 (GRCm39)	D93V	probably benign	Het
Nlrp5	A	G	7: 23,120,880 (GRCm39)	D698G	probably damaging	Het
Or13p10	T	A	4: 118,523,520 (GRCm39)	S269T	probably benign	Het
Pacsin1	T	A	17: 27,924,969 (GRCm39)		probably null	Het
Padi3	A	T	4: 140,518,472 (GRCm39)		probably null	Het
Pcdha8	T	C	18: 37,127,411 (GRCm39)	I631T	probably damaging	Het
Pkn3	G	A	2: 29,972,957 (GRCm39)	G315D	possibly damaging	Het
Ppfibp1	T	A	6: 146,907,422 (GRCm39)	D228E	probably benign	Het
Prag1	G	T	8: 36,613,745 (GRCm39)	R1099L	probably damaging	Het
Pramel31	A	G	4: 144,090,223 (GRCm39)	E421G	probably damaging	Het
Prr36	A	G	8: 4,264,746 (GRCm39)		probably benign	Het
Prrc2c	A	G	1: 162,541,883 (GRCm39)	S369P	probably benign	Het
Ptprn2	A	G	12: 116,839,800 (GRCm39)	D441G	probably benign	Het
Slc9c1	G	A	16: 45,427,204 (GRCm39)		probably benign	Het
Slit1	G	A	19: 41,588,948 (GRCm39)	T1513I	possibly damaging	Het
Smcr8	C	T	11: 60,669,635 (GRCm39)	T261M	probably benign	Het
Spata17	G	A	1: 186,926,151 (GRCm39)	R60*	probably null	Het
Speer4f1	A	G	5: 17,681,241 (GRCm39)	R40G	probably damaging	Het
Spns3	A	G	11: 72,420,466 (GRCm39)	V340A	possibly damaging	Het
Srgap3	T	C	6: 112,716,344 (GRCm39)	I595V	probably benign	Het
Srms	G	A	2: 180,848,038 (GRCm39)	A489V	possibly damaging	Het
St3gal4	T	C	9: 34,964,558 (GRCm39)	N169S	probably damaging	Het
Taar5	T	A	10: 23,847,169 (GRCm39)	V189E	probably damaging	Het
Tbc1d32	G	A	10: 56,071,525 (GRCm39)	P333L	probably benign	Het
Tet3	G	T	6: 83,345,066 (GRCm39)	Y1790*	probably null	Het
Ticrr	T	A	7: 79,344,444 (GRCm39)	H1436Q	probably benign	Het
Top6bl	G	T	19: 4,677,250 (GRCm39)	S466*	probably null	Het
Ubl7	C	T	9: 57,830,555 (GRCm39)	L334F	possibly damaging	Het
Unc13a	A	G	8: 72,119,283 (GRCm39)		probably null	Het
Unc13c	T	C	9: 73,606,451 (GRCm39)	D1303G	probably damaging	Het
Vmn2r114	A	G	17: 23,509,954 (GRCm39)	L842P	possibly damaging	Het
Vps35	G	T	8: 85,987,857 (GRCm39)	Q765K	possibly damaging	Het
Zfp87	G	T	13: 74,520,643 (GRCm39)	S145*	probably null	Het
Zgrf1	C	T	3: 127,392,461 (GRCm39)	A1327V	possibly damaging	Het
Zkscan6	T	A	11: 65,718,983 (GRCm39)	S334R	probably benign	Het

Other mutations in Lama4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Lama4	APN	10	38,941,591 (GRCm39)	splice site	probably benign
IGL00091:Lama4	APN	10	38,948,801 (GRCm39)	missense	probably damaging	1.00
IGL00429:Lama4	APN	10	38,887,022 (GRCm39)	missense	possibly damaging	0.58
IGL00430:Lama4	APN	10	38,921,700 (GRCm39)	missense	possibly damaging	0.54
IGL01074:Lama4	APN	10	38,974,484 (GRCm39)	critical splice donor site	probably null
IGL01386:Lama4	APN	10	38,887,060 (GRCm39)	missense	probably benign	0.00
IGL01603:Lama4	APN	10	38,941,642 (GRCm39)	missense	possibly damaging	0.92
IGL01643:Lama4	APN	10	38,932,846 (GRCm39)	missense	probably benign
IGL01655:Lama4	APN	10	38,936,209 (GRCm39)	missense	probably benign
IGL01954:Lama4	APN	10	38,963,295 (GRCm39)	missense	probably benign	0.05
IGL01984:Lama4	APN	10	38,951,525 (GRCm39)	critical splice donor site	probably null
IGL02193:Lama4	APN	10	38,918,670 (GRCm39)	missense	probably benign
IGL02290:Lama4	APN	10	38,893,360 (GRCm39)	missense	probably benign	0.00
IGL02441:Lama4	APN	10	38,937,441 (GRCm39)	missense	probably benign	0.20
IGL02549:Lama4	APN	10	38,936,200 (GRCm39)	missense	probably benign	0.00
IGL02797:Lama4	APN	10	38,932,920 (GRCm39)	missense	probably null	0.00
IGL02819:Lama4	APN	10	38,902,565 (GRCm39)	missense	possibly damaging	0.80
IGL03122:Lama4	APN	10	38,943,959 (GRCm39)	missense	probably benign
IGL03184:Lama4	APN	10	38,954,839 (GRCm39)	missense	probably damaging	1.00
IGL03307:Lama4	APN	10	38,893,379 (GRCm39)	missense	probably benign
BB006:Lama4	UTSW	10	38,954,843 (GRCm39)	missense	probably damaging	1.00
BB016:Lama4	UTSW	10	38,954,843 (GRCm39)	missense	probably damaging	1.00
PIT4585001:Lama4	UTSW	10	38,950,742 (GRCm39)	missense	probably damaging	1.00
R0003:Lama4	UTSW	10	38,936,218 (GRCm39)	missense	possibly damaging	0.55
R0015:Lama4	UTSW	10	38,951,432 (GRCm39)	missense	possibly damaging	0.87
R0015:Lama4	UTSW	10	38,951,432 (GRCm39)	missense	possibly damaging	0.87
R0035:Lama4	UTSW	10	38,948,734 (GRCm39)	missense	probably benign	0.01
R0141:Lama4	UTSW	10	38,968,274 (GRCm39)	missense	probably benign	0.05
R0257:Lama4	UTSW	10	38,970,880 (GRCm39)	splice site	probably benign
R0267:Lama4	UTSW	10	38,904,635 (GRCm39)	missense	probably damaging	0.96
R0557:Lama4	UTSW	10	38,964,393 (GRCm39)	missense	probably benign	0.38
R1052:Lama4	UTSW	10	38,968,241 (GRCm39)	missense	possibly damaging	0.68
R1248:Lama4	UTSW	10	38,932,843 (GRCm39)	missense	probably damaging	0.99
R1249:Lama4	UTSW	10	38,951,474 (GRCm39)	missense	probably damaging	1.00
R1291:Lama4	UTSW	10	38,924,065 (GRCm39)	missense	probably benign	0.00
R1307:Lama4	UTSW	10	38,946,028 (GRCm39)	missense	probably benign	0.06
R1404:Lama4	UTSW	10	38,937,387 (GRCm39)	missense	probably benign	0.09
R1404:Lama4	UTSW	10	38,937,387 (GRCm39)	missense	probably benign	0.09
R1443:Lama4	UTSW	10	38,949,639 (GRCm39)	missense	probably damaging	1.00
R1499:Lama4	UTSW	10	38,964,876 (GRCm39)	missense	possibly damaging	0.92
R1616:Lama4	UTSW	10	38,951,446 (GRCm39)	missense	probably damaging	1.00
R1691:Lama4	UTSW	10	38,956,559 (GRCm39)	missense	probably benign	0.09
R1748:Lama4	UTSW	10	38,941,615 (GRCm39)	missense	probably benign	0.01
R1768:Lama4	UTSW	10	38,979,497 (GRCm39)	missense	possibly damaging	0.82
R1772:Lama4	UTSW	10	38,936,220 (GRCm39)	missense	probably benign	0.00
R1813:Lama4	UTSW	10	38,936,182 (GRCm39)	missense	probably damaging	1.00
R1813:Lama4	UTSW	10	38,909,121 (GRCm39)	splice site	probably benign
R1897:Lama4	UTSW	10	38,936,182 (GRCm39)	missense	probably damaging	1.00
R1907:Lama4	UTSW	10	38,948,754 (GRCm39)	missense	probably benign	0.13
R1943:Lama4	UTSW	10	38,973,134 (GRCm39)	missense	possibly damaging	0.85
R2041:Lama4	UTSW	10	38,945,987 (GRCm39)	missense	probably damaging	1.00
R2242:Lama4	UTSW	10	38,902,689 (GRCm39)	missense	probably damaging	1.00
R2300:Lama4	UTSW	10	38,963,316 (GRCm39)	missense	probably benign
R2326:Lama4	UTSW	10	38,918,563 (GRCm39)	splice site	probably null
R2570:Lama4	UTSW	10	38,982,043 (GRCm39)	missense	probably damaging	1.00
R2570:Lama4	UTSW	10	38,951,354 (GRCm39)	missense	possibly damaging	0.94
R2571:Lama4	UTSW	10	38,918,671 (GRCm39)	missense	possibly damaging	0.55
R2887:Lama4	UTSW	10	38,968,250 (GRCm39)	missense	possibly damaging	0.94
R2926:Lama4	UTSW	10	38,954,828 (GRCm39)	missense	probably benign	0.16
R3237:Lama4	UTSW	10	38,973,175 (GRCm39)	missense	probably damaging	0.97
R4095:Lama4	UTSW	10	38,973,118 (GRCm39)	missense	probably damaging	1.00
R4151:Lama4	UTSW	10	38,881,424 (GRCm39)	missense	probably benign	0.00
R4470:Lama4	UTSW	10	38,956,492 (GRCm39)	nonsense	probably null
R4812:Lama4	UTSW	10	38,948,765 (GRCm39)	missense	probably benign
R4822:Lama4	UTSW	10	38,909,049 (GRCm39)	missense	probably benign	0.01
R4997:Lama4	UTSW	10	38,968,262 (GRCm39)	missense	probably damaging	0.99
R5119:Lama4	UTSW	10	38,924,050 (GRCm39)	missense	probably benign	0.00
R5468:Lama4	UTSW	10	38,948,678 (GRCm39)	splice site	probably null
R5909:Lama4	UTSW	10	38,948,855 (GRCm39)	missense	probably benign	0.00
R5917:Lama4	UTSW	10	38,924,028 (GRCm39)	missense	probably benign	0.10
R5927:Lama4	UTSW	10	38,948,808 (GRCm39)	missense	probably damaging	1.00
R5950:Lama4	UTSW	10	38,906,444 (GRCm39)	missense	probably benign	0.03
R6051:Lama4	UTSW	10	38,943,898 (GRCm39)	missense	probably benign	0.01
R6294:Lama4	UTSW	10	38,951,466 (GRCm39)	missense	probably damaging	1.00
R6372:Lama4	UTSW	10	38,943,948 (GRCm39)	missense	probably benign
R6532:Lama4	UTSW	10	38,924,073 (GRCm39)	missense	possibly damaging	0.58
R6547:Lama4	UTSW	10	38,949,652 (GRCm39)	missense	probably damaging	1.00
R6578:Lama4	UTSW	10	38,893,361 (GRCm39)	missense	probably benign	0.01
R6737:Lama4	UTSW	10	38,970,907 (GRCm39)	missense	probably damaging	0.96
R6987:Lama4	UTSW	10	38,950,275 (GRCm39)	missense	probably benign	0.00
R7040:Lama4	UTSW	10	38,936,158 (GRCm39)	missense	possibly damaging	0.69
R7139:Lama4	UTSW	10	38,951,491 (GRCm39)	missense	probably damaging	1.00
R7188:Lama4	UTSW	10	38,841,729 (GRCm39)	start gained	probably benign
R7189:Lama4	UTSW	10	38,841,729 (GRCm39)	start gained	probably benign
R7199:Lama4	UTSW	10	38,956,536 (GRCm39)	missense	possibly damaging	0.84
R7211:Lama4	UTSW	10	38,881,491 (GRCm39)	missense	probably damaging	0.98
R7262:Lama4	UTSW	10	38,970,930 (GRCm39)	missense	probably damaging	1.00
R7274:Lama4	UTSW	10	38,968,295 (GRCm39)	missense	probably benign	0.00
R7311:Lama4	UTSW	10	38,902,631 (GRCm39)	missense	probably damaging	1.00
R7391:Lama4	UTSW	10	38,963,383 (GRCm39)	critical splice donor site	probably null
R7399:Lama4	UTSW	10	38,923,944 (GRCm39)	missense	probably damaging	0.98
R7426:Lama4	UTSW	10	38,921,751 (GRCm39)	missense	possibly damaging	0.82
R7472:Lama4	UTSW	10	38,963,369 (GRCm39)	missense	possibly damaging	0.65
R7635:Lama4	UTSW	10	38,968,184 (GRCm39)	missense	probably benign
R7775:Lama4	UTSW	10	38,954,843 (GRCm39)	missense	probably damaging	1.00
R7805:Lama4	UTSW	10	38,902,747 (GRCm39)	critical splice donor site	probably null
R7885:Lama4	UTSW	10	38,964,840 (GRCm39)	missense	probably benign	0.01
R7895:Lama4	UTSW	10	38,964,325 (GRCm39)	missense	probably damaging	0.96
R7910:Lama4	UTSW	10	38,946,005 (GRCm39)	missense	probably damaging	0.99
R7929:Lama4	UTSW	10	38,954,843 (GRCm39)	missense	probably damaging	1.00
R7952:Lama4	UTSW	10	38,906,486 (GRCm39)	missense	probably benign	0.39
R7991:Lama4	UTSW	10	38,921,805 (GRCm39)	missense	possibly damaging	0.70
R8059:Lama4	UTSW	10	38,842,057 (GRCm39)	missense	probably benign	0.00
R8194:Lama4	UTSW	10	38,954,716 (GRCm39)	missense	probably damaging	0.99
R8248:Lama4	UTSW	10	38,937,375 (GRCm39)	missense	possibly damaging	0.82
R8252:Lama4	UTSW	10	38,936,142 (GRCm39)	missense	probably benign	0.00
R8265:Lama4	UTSW	10	38,981,200 (GRCm39)	missense	probably damaging	1.00
R8275:Lama4	UTSW	10	38,948,807 (GRCm39)	missense	probably damaging	1.00
R8426:Lama4	UTSW	10	38,979,487 (GRCm39)	missense	probably damaging	0.98
R8434:Lama4	UTSW	10	38,902,703 (GRCm39)	missense	possibly damaging	0.92
R8720:Lama4	UTSW	10	38,971,079 (GRCm39)	missense	probably damaging	0.97
R8792:Lama4	UTSW	10	38,924,048 (GRCm39)	missense	probably benign	0.00
R8836:Lama4	UTSW	10	38,902,587 (GRCm39)	missense	probably damaging	1.00
R8867:Lama4	UTSW	10	38,923,996 (GRCm39)	missense	probably damaging	1.00
R8892:Lama4	UTSW	10	38,973,194 (GRCm39)	missense	probably damaging	1.00
R8913:Lama4	UTSW	10	38,982,039 (GRCm39)	missense	probably benign	0.10
R9129:Lama4	UTSW	10	38,932,887 (GRCm39)	missense	probably benign
R9177:Lama4	UTSW	10	38,950,688 (GRCm39)	missense	probably damaging	0.98
R9187:Lama4	UTSW	10	38,924,124 (GRCm39)	critical splice donor site	probably null
R9193:Lama4	UTSW	10	38,951,444 (GRCm39)	missense	probably benign	0.03
R9268:Lama4	UTSW	10	38,950,688 (GRCm39)	missense	probably damaging	0.98
R9287:Lama4	UTSW	10	38,981,960 (GRCm39)	missense	probably damaging	1.00
R9295:Lama4	UTSW	10	38,948,747 (GRCm39)	missense	probably damaging	1.00
R9303:Lama4	UTSW	10	38,973,137 (GRCm39)	missense	probably damaging	0.99
R9330:Lama4	UTSW	10	38,954,722 (GRCm39)	missense	probably damaging	0.99
R9430:Lama4	UTSW	10	38,921,802 (GRCm39)	missense	probably null
R9572:Lama4	UTSW	10	38,959,271 (GRCm39)	missense	probably damaging	1.00
R9636:Lama4	UTSW	10	38,956,500 (GRCm39)	missense	possibly damaging	0.67
R9663:Lama4	UTSW	10	38,923,944 (GRCm39)	missense	probably damaging	0.98
R9777:Lama4	UTSW	10	38,924,101 (GRCm39)	missense	probably benign	0.00
X0067:Lama4	UTSW	10	38,921,688 (GRCm39)	missense	probably benign	0.00
Z1177:Lama4	UTSW	10	38,881,421 (GRCm39)	missense	probably damaging	1.00
Z1177:Lama4	UTSW	10	38,881,420 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AATCTGATTGTCCCCTTGTCTAAG -3'
(R):5'- GTGAGTTACGAAAATACAAGGCTCAC -3'

Sequencing Primer
(F):5'- ATGCTTCGACATTATTGCTGTATTC -3'
(R):5'- AGGCTCACTTCTTAATATAACAACAG -3'

Posted On

2018-03-15