Mutagenetix > Incidental Mutation

Incidental Mutation 'R6278:Or5w14'

507762

Institutional Source

Beutler Lab

Gene Symbol

Or5w14

Ensembl Gene

ENSMUSG00000075150

Gene Name

olfactory receptor family 5 subfamily W member 14

Synonyms

Olfr1137, MOR177-20, GA_x6K02T2Q125-49215724-49214792, MOR40-9P

MMRRC Submission

044448-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R6278 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87541316-87542248 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 87541815 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 145 (L145*)

Ref Sequence

ENSEMBL: ENSMUSP00000149311 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099850] [ENSMUST00000214209]

AlphaFold

Q7TR40

Predicted Effect

probably null
Transcript: ENSMUST00000099850
AA Change: L145*

SMART Domains

Protein: ENSMUSP00000097437
Gene: ENSMUSG00000075150
AA Change: L145*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-45	PFAM
Pfam:7tm_1	41	290	2.8e-17	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000214209
AA Change: L145*

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.8%
20x: 93.6%

Validation Efficiency

100% (49/49)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsm3	T	C	7: 119,373,072 (GRCm39)	S192P	probably damaging	Het
Amy1	T	A	3: 113,355,339 (GRCm39)	Y348F	probably damaging	Het
Brpf3	C	A	17: 29,040,258 (GRCm39)	P893H	probably benign	Het
Cage1	T	C	13: 38,200,395 (GRCm39)	T702A	possibly damaging	Het
Cdk11b	A	G	4: 155,734,060 (GRCm39)		probably benign	Het
Cdk5rap3	T	C	11: 96,802,729 (GRCm39)	Y207C	probably damaging	Het
Cenpc1	T	C	5: 86,183,394 (GRCm39)	K465R	probably damaging	Het
Cldn14	A	C	16: 93,716,486 (GRCm39)	L120R	possibly damaging	Het
Cyp2c69	A	T	19: 39,831,507 (GRCm39)	C435*	probably null	Het
Dmxl2	T	A	9: 54,323,046 (GRCm39)	E1446V	probably damaging	Het
Dnah17	T	A	11: 118,017,116 (GRCm39)	D208V	probably damaging	Het
Eif4g3	G	T	4: 137,915,394 (GRCm39)	G1430V	possibly damaging	Het
Eif5	T	A	12: 111,509,227 (GRCm39)	D284E	probably benign	Het
Fam47e	T	C	5: 92,710,376 (GRCm39)	L125P	probably damaging	Het
Far1	A	G	7: 113,167,344 (GRCm39)	I476M	probably benign	Het
Fsip2	A	T	2: 82,819,242 (GRCm39)	I4992L	probably benign	Het
Gli3	T	A	13: 15,899,698 (GRCm39)	N1028K	possibly damaging	Het
Gucy1a1	T	C	3: 82,004,941 (GRCm39)	K615E	probably damaging	Het
Gykl1	G	T	18: 52,828,280 (GRCm39)	S496I	probably benign	Het
Hmcn1	C	T	1: 150,573,170 (GRCm39)		probably null	Het
Itga2	T	A	13: 114,982,424 (GRCm39)	H1027L	probably benign	Het
Kif24	A	T	4: 41,423,498 (GRCm39)	V251E	probably damaging	Het
Nupr1	T	C	7: 126,224,518 (GRCm39)	Y30C	probably damaging	Het
Or5b119	T	A	19: 13,457,119 (GRCm39)	M148L	probably benign	Het
Or5g29	A	G	2: 85,421,342 (GRCm39)	I153V	probably benign	Het
Or8g26	T	A	9: 39,095,594 (GRCm39)	I40N	probably damaging	Het
P2ry1	T	A	3: 60,911,215 (GRCm39)	I118N	possibly damaging	Het
Pcdh1	C	T	18: 38,332,263 (GRCm39)	V247M	probably benign	Het
Pdia5	A	G	16: 35,250,293 (GRCm39)	V222A	possibly damaging	Het
Plce1	G	A	19: 38,713,495 (GRCm39)		probably null	Het
Ppm1m	T	C	9: 106,074,427 (GRCm39)	R239G	probably damaging	Het
Pramel30	C	T	4: 144,056,837 (GRCm39)	P7S	probably damaging	Het
Prkcz	A	T	4: 155,352,652 (GRCm39)	F492I	probably damaging	Het
Rpl6	T	C	5: 121,346,912 (GRCm39)	I269T	possibly damaging	Het
Scoc	T	C	8: 84,184,965 (GRCm39)	S2G	unknown	Het
Spesp1	A	T	9: 62,179,921 (GRCm39)	M329K	probably benign	Het
Ston2	T	C	12: 91,615,104 (GRCm39)	K435E	probably damaging	Het
Synj2	A	T	17: 6,026,149 (GRCm39)	L69F	probably damaging	Het
Tigd5	T	C	15: 75,781,842 (GRCm39)	I68T	probably damaging	Het
Tmcc2	A	T	1: 132,286,720 (GRCm39)	M577K	probably damaging	Het
Tmem131l	A	T	3: 83,849,798 (GRCm39)	I263N	possibly damaging	Het
Trip13	T	C	13: 74,061,439 (GRCm39)	E408G	probably benign	Het
Txnl4b	T	C	8: 110,295,735 (GRCm39)		probably null	Het
Ube2o	T	C	11: 116,430,369 (GRCm39)	E1123G	probably damaging	Het
Vmn1r123	A	G	7: 20,896,774 (GRCm39)	H222R	possibly damaging	Het
Vmn2r73	G	T	7: 85,522,140 (GRCm39)	H66Q	probably benign	Het
Zc3h13	T	A	14: 75,567,863 (GRCm39)	V1052D	probably benign	Het

Other mutations in Or5w14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Or5w14	APN	2	87,541,477 (GRCm39)	missense	possibly damaging	0.76
IGL02225:Or5w14	APN	2	87,541,743 (GRCm39)	missense	possibly damaging	0.78
IGL02312:Or5w14	APN	2	87,541,353 (GRCm39)	missense	probably benign	0.00
IGL03326:Or5w14	APN	2	87,542,039 (GRCm39)	missense	probably damaging	1.00
R0432:Or5w14	UTSW	2	87,541,774 (GRCm39)	missense	probably benign	0.13
R1656:Or5w14	UTSW	2	87,541,422 (GRCm39)	missense	possibly damaging	0.94
R1852:Or5w14	UTSW	2	87,541,317 (GRCm39)	splice site	probably null
R1880:Or5w14	UTSW	2	87,541,639 (GRCm39)	missense	probably damaging	1.00
R2184:Or5w14	UTSW	2	87,541,549 (GRCm39)	missense	probably damaging	1.00
R2511:Or5w14	UTSW	2	87,541,392 (GRCm39)	missense	probably damaging	1.00
R4356:Or5w14	UTSW	2	87,542,229 (GRCm39)	missense	possibly damaging	0.95
R6184:Or5w14	UTSW	2	87,542,188 (GRCm39)	missense	probably benign
R6621:Or5w14	UTSW	2	87,541,899 (GRCm39)	missense	probably benign	0.10
R7549:Or5w14	UTSW	2	87,542,115 (GRCm39)	missense	probably damaging	1.00
R7799:Or5w14	UTSW	2	87,541,428 (GRCm39)	missense	possibly damaging	0.88
R8187:Or5w14	UTSW	2	87,541,624 (GRCm39)	missense	probably benign	0.14
R8236:Or5w14	UTSW	2	87,542,104 (GRCm39)	missense	possibly damaging	0.63
R8298:Or5w14	UTSW	2	87,541,376 (GRCm39)	missense	probably damaging	1.00
R8314:Or5w14	UTSW	2	87,541,546 (GRCm39)	missense	probably benign	0.00
R8398:Or5w14	UTSW	2	87,542,175 (GRCm39)	missense	probably benign
R9121:Or5w14	UTSW	2	87,541,975 (GRCm39)	missense	probably damaging	0.99
R9378:Or5w14	UTSW	2	87,541,423 (GRCm39)	missense	possibly damaging	0.88
R9553:Or5w14	UTSW	2	87,541,992 (GRCm39)	missense	probably benign	0.10
R9732:Or5w14	UTSW	2	87,541,489 (GRCm39)	missense	possibly damaging	0.78

Predicted Primers

PCR Primer
(F):5'- GACACTTGAGATTGTGCTCAGTTC -3'
(R):5'- TGCTGGTAGACCTAGTAGCC -3'

Sequencing Primer
(F):5'- TGTGCTCAGTTCAATGAAACC -3'
(R):5'- GTAGACCTAGTAGCCGAGAAACATTC -3'

Posted On

2018-03-15