Mutagenetix > Incidental Mutation

Incidental Mutation 'R6286:Vmn2r84'

508240

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r84

Ensembl Gene

ENSMUSG00000070601

Gene Name

vomeronasal 2, receptor 84

Synonyms

EG625068

MMRRC Submission

044456-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R6286 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

130221669-130230110 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 130226737 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Arginine at position 367 (M367R)

Ref Sequence

ENSEMBL: ENSMUSP00000092079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094502]

AlphaFold

D3YWE3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094502
AA Change: M367R

PolyPhen 2 Score 0.655 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000092079
Gene: ENSMUSG00000070601
AA Change: M367R

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	77	448	1.3e-27	PFAM
Pfam:NCD3G	508	561	6.9e-21	PFAM
Pfam:7tm_3	594	830	4.6e-55	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.7%

Validation Efficiency

98% (40/41)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alk	T	A	17: 72,187,842 (GRCm39)	M1300L	probably damaging	Het
C3	A	T	17: 57,531,118 (GRCm39)	I356N	probably damaging	Het
Cntnap5b	T	A	1: 100,182,798 (GRCm39)	S276T	possibly damaging	Het
Cts7	C	T	13: 61,500,584 (GRCm39)	G321E	probably damaging	Het
Cyp11a1	G	A	9: 57,924,701 (GRCm39)		probably benign	Het
Ddx4	T	C	13: 112,750,269 (GRCm39)	T421A	probably damaging	Het
Dnttip2	A	G	3: 122,078,049 (GRCm39)	T694A	probably damaging	Het
Eif3b	G	T	5: 140,405,566 (GRCm39)	D151Y	probably damaging	Het
Fhad1	T	A	4: 141,648,209 (GRCm39)	E219V	probably damaging	Het
Gdf2	C	T	14: 33,667,057 (GRCm39)	R260C	probably damaging	Het
Gm14295	T	C	2: 176,501,361 (GRCm39)	Y284H	possibly damaging	Het
Gm45861	A	G	8: 28,019,619 (GRCm39)	T745A	unknown	Het
Grin2a	G	A	16: 9,579,639 (GRCm39)	T208I	possibly damaging	Het
Ide	T	A	19: 37,255,409 (GRCm39)	Y798F	unknown	Het
Llgl1	G	A	11: 60,600,358 (GRCm39)	G569D	probably damaging	Het
Mrpl9	G	C	3: 94,351,097 (GRCm39)	E92D	probably benign	Het
Muc16	T	A	9: 18,555,685 (GRCm39)	H3536L	unknown	Het
Nasp	T	C	4: 116,461,985 (GRCm39)	Y526C	probably damaging	Het
Nfx1	A	G	4: 40,986,728 (GRCm39)	N404D	probably damaging	Het
Nsrp1	A	G	11: 76,940,269 (GRCm39)	I112T	probably damaging	Het
Or6c7	T	C	10: 129,323,497 (GRCm39)	L206P	probably damaging	Het
Or8b8	A	T	9: 37,809,074 (GRCm39)	I125F	probably damaging	Het
Oxsr1	A	G	9: 119,093,948 (GRCm39)	V235A	probably damaging	Het
Pamr1	C	T	2: 102,471,293 (GRCm39)	Q539*	probably null	Het
Pramel20	T	A	4: 143,297,796 (GRCm39)	V72D	probably benign	Het
Ptpn4	A	G	1: 119,649,592 (GRCm39)		probably null	Het
Ranbp2	C	T	10: 58,315,394 (GRCm39)	A2038V	probably benign	Het
Rsf1	G	GACGGCGGCA	7: 97,229,116 (GRCm39)		probably benign	Homo
Septin5	A	G	16: 18,442,127 (GRCm39)	V253A	probably damaging	Het
Skic3	T	C	13: 76,291,359 (GRCm39)	L993P	probably damaging	Het
Slc22a13	C	A	9: 119,037,778 (GRCm39)	E117*	probably null	Het
Slc9c1	T	C	16: 45,398,194 (GRCm39)	I653T	probably benign	Het
Slco6c1	T	A	1: 97,053,445 (GRCm39)	H152L	possibly damaging	Het
Sp2	A	C	11: 96,852,372 (GRCm39)	V184G	probably benign	Het
Taok1	T	A	11: 77,444,599 (GRCm39)	H492L	probably benign	Het
Tas2r110	G	A	6: 132,845,490 (GRCm39)	D174N	probably benign	Het
Trim24	G	A	6: 37,896,426 (GRCm39)		probably null	Het
Ttn	G	A	2: 76,581,321 (GRCm39)	R21445*	probably null	Het
Ttn	A	G	2: 76,605,967 (GRCm39)	Y16502H	probably damaging	Het
Zfp46	T	C	4: 136,018,320 (GRCm39)	S385P	probably damaging	Het

Other mutations in Vmn2r84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Vmn2r84	APN	10	130,227,094 (GRCm39)	missense	possibly damaging	0.65
IGL01590:Vmn2r84	APN	10	130,221,964 (GRCm39)	missense	probably damaging	1.00
IGL01639:Vmn2r84	APN	10	130,225,141 (GRCm39)	nonsense	probably null
IGL01843:Vmn2r84	APN	10	130,222,148 (GRCm39)	missense	probably benign
IGL01911:Vmn2r84	APN	10	130,222,277 (GRCm39)	missense	probably damaging	0.99
IGL01937:Vmn2r84	APN	10	130,221,755 (GRCm39)	missense	probably damaging	1.00
IGL01977:Vmn2r84	APN	10	130,229,935 (GRCm39)	missense	probably benign	0.11
IGL02177:Vmn2r84	APN	10	130,227,881 (GRCm39)	missense	probably benign	0.00
IGL02291:Vmn2r84	APN	10	130,226,617 (GRCm39)	missense	probably damaging	1.00
IGL02590:Vmn2r84	APN	10	130,227,356 (GRCm39)	splice site	probably benign
IGL02727:Vmn2r84	APN	10	130,229,995 (GRCm39)	missense	possibly damaging	0.95
IGL02900:Vmn2r84	APN	10	130,223,861 (GRCm39)	splice site	probably benign
IGL03383:Vmn2r84	APN	10	130,222,556 (GRCm39)	missense	probably damaging	1.00
PIT4378001:Vmn2r84	UTSW	10	130,221,784 (GRCm39)	missense	probably damaging	1.00
R0076:Vmn2r84	UTSW	10	130,230,062 (GRCm39)	missense	probably damaging	1.00
R0089:Vmn2r84	UTSW	10	130,222,588 (GRCm39)	splice site	probably benign
R0153:Vmn2r84	UTSW	10	130,227,877 (GRCm39)	missense	probably benign	0.06
R0611:Vmn2r84	UTSW	10	130,221,991 (GRCm39)	missense	probably damaging	1.00
R0883:Vmn2r84	UTSW	10	130,226,984 (GRCm39)	missense	probably damaging	0.99
R1237:Vmn2r84	UTSW	10	130,223,725 (GRCm39)	splice site	probably null
R1295:Vmn2r84	UTSW	10	130,225,008 (GRCm39)	missense	probably benign	0.12
R1401:Vmn2r84	UTSW	10	130,227,859 (GRCm39)	missense	possibly damaging	0.89
R1521:Vmn2r84	UTSW	10	130,225,137 (GRCm39)	missense	probably benign	0.10
R1590:Vmn2r84	UTSW	10	130,227,349 (GRCm39)	critical splice acceptor site	probably null
R1710:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R1891:Vmn2r84	UTSW	10	130,221,938 (GRCm39)	missense	possibly damaging	0.78
R1956:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1957:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1962:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
R1994:Vmn2r84	UTSW	10	130,221,878 (GRCm39)	missense	probably damaging	1.00
R2124:Vmn2r84	UTSW	10	130,227,100 (GRCm39)	missense	probably damaging	0.99
R2409:Vmn2r84	UTSW	10	130,227,940 (GRCm39)	missense	probably damaging	0.99
R2474:Vmn2r84	UTSW	10	130,222,392 (GRCm39)	missense	possibly damaging	0.50
R2851:Vmn2r84	UTSW	10	130,230,036 (GRCm39)	missense	probably benign	0.05
R3508:Vmn2r84	UTSW	10	130,226,777 (GRCm39)	missense	probably damaging	1.00
R3792:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R4051:Vmn2r84	UTSW	10	130,226,767 (GRCm39)	missense	probably damaging	1.00
R4061:Vmn2r84	UTSW	10	130,221,898 (GRCm39)	missense	probably damaging	1.00
R4091:Vmn2r84	UTSW	10	130,227,238 (GRCm39)	missense	probably damaging	1.00
R4190:Vmn2r84	UTSW	10	130,227,163 (GRCm39)	nonsense	probably null
R4520:Vmn2r84	UTSW	10	130,222,391 (GRCm39)	missense	probably damaging	1.00
R4584:Vmn2r84	UTSW	10	130,226,582 (GRCm39)	missense	probably benign	0.00
R4588:Vmn2r84	UTSW	10	130,221,809 (GRCm39)	missense	probably damaging	0.98
R4655:Vmn2r84	UTSW	10	130,229,973 (GRCm39)	nonsense	probably null
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R5022:Vmn2r84	UTSW	10	130,222,417 (GRCm39)	missense	probably damaging	1.00
R5146:Vmn2r84	UTSW	10	130,221,971 (GRCm39)	missense	probably damaging	1.00
R5237:Vmn2r84	UTSW	10	130,221,863 (GRCm39)	missense	probably damaging	0.99
R5695:Vmn2r84	UTSW	10	130,225,064 (GRCm39)	missense	probably benign	0.12
R5793:Vmn2r84	UTSW	10	130,221,754 (GRCm39)	missense	probably damaging	0.99
R6210:Vmn2r84	UTSW	10	130,222,114 (GRCm39)	missense	probably damaging	1.00
R6580:Vmn2r84	UTSW	10	130,225,110 (GRCm39)	missense	possibly damaging	0.93
R6607:Vmn2r84	UTSW	10	130,226,731 (GRCm39)	missense	possibly damaging	0.87
R6818:Vmn2r84	UTSW	10	130,222,147 (GRCm39)	missense	probably benign	0.09
R6956:Vmn2r84	UTSW	10	130,225,136 (GRCm39)	missense	probably damaging	0.98
R6994:Vmn2r84	UTSW	10	130,226,876 (GRCm39)	missense	possibly damaging	0.90
R7075:Vmn2r84	UTSW	10	130,226,941 (GRCm39)	missense	probably damaging	0.99
R7225:Vmn2r84	UTSW	10	130,222,552 (GRCm39)	missense	probably damaging	0.99
R7252:Vmn2r84	UTSW	10	130,222,279 (GRCm39)	missense	probably damaging	1.00
R7263:Vmn2r84	UTSW	10	130,225,077 (GRCm39)	missense	probably damaging	1.00
R7297:Vmn2r84	UTSW	10	130,227,119 (GRCm39)	missense	probably benign	0.19
R7439:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7441:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7857:Vmn2r84	UTSW	10	130,226,738 (GRCm39)	missense	probably benign	0.00
R8263:Vmn2r84	UTSW	10	130,227,037 (GRCm39)	missense	probably damaging	1.00
R8555:Vmn2r84	UTSW	10	130,230,100 (GRCm39)	missense	probably benign	0.28
R8766:Vmn2r84	UTSW	10	130,222,241 (GRCm39)	missense	probably damaging	0.98
R8821:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8831:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8970:Vmn2r84	UTSW	10	130,222,244 (GRCm39)	missense	probably damaging	0.98
R9164:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R9190:Vmn2r84	UTSW	10	130,226,843 (GRCm39)	missense	probably benign	0.03
R9261:Vmn2r84	UTSW	10	130,229,976 (GRCm39)	missense	probably benign	0.00
R9310:Vmn2r84	UTSW	10	130,227,993 (GRCm39)	missense	possibly damaging	0.81
R9434:Vmn2r84	UTSW	10	130,221,745 (GRCm39)	missense	possibly damaging	0.65
R9613:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r84	UTSW	10	130,227,771 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTTTACCTGCTGACATTCTGTG -3'
(R):5'- TCACAACCTCACAATGGGATG -3'

Sequencing Primer
(F):5'- ACCTGCTGACATTCTGTGTATTC -3'
(R):5'- ACCTCACAATGGGATGTTATCAC -3'

Posted On

2018-03-15