Mutagenetix > Incidental Mutation

Incidental Mutation 'R6291:Scn7a'

508394

Institutional Source

Beutler Lab

Gene Symbol

Scn7a

Ensembl Gene

ENSMUSG00000034810

Gene Name

sodium channel, voltage-gated, type VII, alpha

Synonyms

NaG, Nav2, Nav2.3, Nax, Scn6a

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R6291 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66673425-66784914 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 66700114 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 629 (D629V)

Ref Sequence

ENSEMBL: ENSMUSP00000042405 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042792]

AlphaFold

B1AYL1

Predicted Effect

probably damaging
Transcript: ENSMUST00000042792
AA Change: D629V

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000042405
Gene: ENSMUSG00000034810
AA Change: D629V

Domain	Start	End	E-Value	Type
Pfam:Ion_trans	118	405	4.7e-53	PFAM
coiled coil region	415	443	N/A	INTRINSIC
Pfam:Ion_trans	505	739	5.8e-36	PFAM
Pfam:Na_trans_assoc	741	929	4.1e-17	PFAM
Pfam:Ion_trans	933	1204	3e-49	PFAM
Pfam:Ion_trans	1250	1505	5e-37	PFAM
IQ	1624	1646	6.4e-2	SMART

Meta Mutation Damage Score

0.3871

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.3%

Validation Efficiency

98% (86/88)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the many voltage-gated sodium channel proteins. For proper functioning of neurons and muscles during action potentials, voltage-gated sodium channels direct sodium ion diffusion for membrane depolarization. This sodium channel protein has some atypical characteristics; the similarity between the human and mouse proteins is lower compared to other orthologous sodium channel pairs. Also, the S4 segments, which sense voltage changes, have fewer positive charged residues that in other sodium channels; domain 4 has fewer arginine and lysine residues compared to other sodium channel proteins. Several alternatively spliced transcript variants exist, but the full-length natures of all of them remain unknown. [provided by RefSeq, Dec 2011]
PHENOTYPE: Mice homozygous for disruptions in this gene have a modified dietary preference for NaCl but are phenotypically normal otherwise. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc12	C	T	8: 86,566,544 (GRCm38)	G5D	possibly damaging	Het
Adamts9	T	C	6: 92,890,120 (GRCm38)	K95R	probably damaging	Het
Adap1	G	T	5: 139,273,491 (GRCm38)	L314M	probably benign	Het
Alkbh1	T	A	12: 87,429,094 (GRCm38)	E306V	possibly damaging	Het
Alpk2	T	A	18: 65,305,901 (GRCm38)	D1274V	possibly damaging	Het
Ankrd31	A	C	13: 96,878,238 (GRCm38)	K1188N	possibly damaging	Het
Aox2	T	A	1: 58,330,806 (GRCm38)	M759K	probably damaging	Het
Atp13a3	T	A	16: 30,336,243 (GRCm38)	D961V	probably damaging	Het
BC067074	T	A	13: 113,320,447 (GRCm38)	I1009N	possibly damaging	Het
Bcl11a	G	A	11: 24,158,321 (GRCm38)	G100R	probably damaging	Het
Bpifc	G	A	10: 85,976,258 (GRCm38)	A362V	probably damaging	Het
Btaf1	C	T	19: 36,973,008 (GRCm38)	T546I	probably benign	Het
Casd1	C	A	6: 4,619,834 (GRCm38)	P193Q	probably damaging	Het
Cdhr1	T	C	14: 37,089,465 (GRCm38)	T230A	probably benign	Het
Celsr3	T	G	9: 108,828,842 (GRCm38)	D841E	probably damaging	Het
Cenpj	T	C	14: 56,551,976 (GRCm38)	D872G	probably benign	Het
Cep95	G	T	11: 106,815,596 (GRCm38)	A559S	probably damaging	Het
Chpt1	A	T	10: 88,475,444 (GRCm38)	C62*	probably null	Het
Cspp1	A	G	1: 10,064,334 (GRCm38)	K103R	probably damaging	Het
Ctla4	T	C	1: 60,912,678 (GRCm38)	V122A	probably benign	Het
Cyp3a11	A	G	5: 145,862,427 (GRCm38)	F317L	possibly damaging	Het
Daam1	T	C	12: 71,946,251 (GRCm38)	L338P	unknown	Het
Dcc	T	A	18: 71,682,167 (GRCm38)	I379L	probably benign	Het
Dennd2c	C	A	3: 103,131,609 (GRCm38)	C24*	probably null	Het
Dnajc12	A	G	10: 63,397,274 (GRCm38)	I65V	probably benign	Het
Dock3	T	A	9: 106,908,432 (GRCm38)	M208L	probably benign	Het
Dsg1b	T	C	18: 20,404,791 (GRCm38)	I588T	possibly damaging	Het
Eif1b	T	C	9: 120,494,140 (GRCm38)	L22S	probably benign	Het
Ep300	T	A	15: 81,648,507 (GRCm38)	S1649T	unknown	Het
Eps15	CAAA	CAA	4: 109,305,703 (GRCm38)		probably null	Het
Ercc6	A	T	14: 32,569,986 (GRCm38)	E1102D	probably benign	Het
Gsdmc3	T	C	15: 63,860,241 (GRCm38)	N312S	probably benign	Het
Guca2b	A	T	4: 119,657,693 (GRCm38)	L57Q	probably damaging	Het
Heatr5b	G	T	17: 78,762,097 (GRCm38)	H1740Q	probably benign	Het
Hecw1	G	A	13: 14,523,007 (GRCm38)		probably benign	Het
Icam5	A	G	9: 21,036,921 (GRCm38)	H675R	probably benign	Het
Il18rap	T	C	1: 40,524,889 (GRCm38)	F56L	probably benign	Het
Iqgap3	T	A	3: 88,089,730 (GRCm38)		probably null	Het
Itsn1	T	C	16: 91,868,096 (GRCm38)		probably benign	Het
Jakmip3	A	T	7: 139,020,856 (GRCm38)	D315V	probably damaging	Het
Kif24	A	G	4: 41,413,959 (GRCm38)	Y328H	probably damaging	Het
Kmt2a	A	T	9: 44,832,874 (GRCm38)		probably benign	Het
Kng1	A	G	16: 23,079,725 (GRCm38)	E625G	probably damaging	Het
Man2b1	C	T	8: 85,097,046 (GRCm38)	T973I	probably benign	Het
Masp2	G	A	4: 148,602,753 (GRCm38)	V31M	probably damaging	Het
Myo18b	C	T	5: 112,865,735 (GRCm38)	R785H	possibly damaging	Het
Naa15	G	A	3: 51,442,791 (GRCm38)	G103D	probably damaging	Het
Olfr1029	A	G	2: 85,975,582 (GRCm38)	Y113C	probably damaging	Het
Olfr1309	A	G	2: 111,983,624 (GRCm38)	V150A	probably benign	Het
Olfr873	T	A	9: 20,300,603 (GRCm38)	D134E	probably damaging	Het
Papln	A	G	12: 83,783,015 (GRCm38)	N970S	probably benign	Het
Pick1	T	A	15: 79,251,728 (GRCm38)		probably null	Het
Pigr	A	C	1: 130,841,761 (GRCm38)	D103A	probably benign	Het
Plekhf1	A	C	7: 38,221,605 (GRCm38)	F180V	possibly damaging	Het
Plxnc1	A	G	10: 94,833,642 (GRCm38)		probably null	Het
Polr3f	A	G	2: 144,534,388 (GRCm38)	I136V	probably damaging	Het
Ppp2r2c	T	C	5: 36,940,124 (GRCm38)	M218T	possibly damaging	Het
Prox2	C	T	12: 85,089,646 (GRCm38)	V466I	probably damaging	Het
Prrc2a	G	A	17: 35,154,933 (GRCm38)	L1479F	probably damaging	Het
Rbm24	T	A	13: 46,421,837 (GRCm38)		probably null	Het
Rcc1l	A	T	5: 134,166,721 (GRCm38)		probably null	Het
Ripk4	A	C	16: 97,755,123 (GRCm38)	L140R	probably damaging	Het
Rmnd1	A	T	10: 4,422,135 (GRCm38)	L188Q	probably damaging	Het
Rnf170	C	T	8: 26,140,964 (GRCm38)	P249S	probably damaging	Het
Rras	A	G	7: 45,018,171 (GRCm38)		probably null	Het
Rsf1	G	A	7: 97,579,910 (GRCm38)		probably benign	Het
Sipa1l3	A	G	7: 29,388,133 (GRCm38)	S556P	probably damaging	Het
Smarca2	C	T	19: 26,630,892 (GRCm38)	A117V	probably damaging	Het
Snca	C	T	6: 60,815,718 (GRCm38)	A69T	probably damaging	Het
Snx2	T	C	18: 53,209,665 (GRCm38)		probably null	Het
Spp1	T	A	5: 104,439,376 (GRCm38)	S109T	possibly damaging	Het
Stoml3	T	C	3: 53,507,516 (GRCm38)	L243P	probably damaging	Het
Susd2	A	G	10: 75,637,574 (GRCm38)	F789L	possibly damaging	Het
Sycp1	A	G	3: 102,908,961 (GRCm38)	M419T	probably damaging	Het
Thoc1	T	C	18: 9,993,330 (GRCm38)	V563A	probably benign	Het
Tmprss11g	T	C	5: 86,487,422 (GRCm38)	I398V	probably damaging	Het
Tox3	A	G	8: 90,248,938 (GRCm38)	L355P	probably damaging	Het
Tpp1	A	G	7: 105,747,016 (GRCm38)	I492T	probably benign	Het
Trim21	A	T	7: 102,564,082 (GRCm38)	L3Q	probably damaging	Het
Ttn	T	C	2: 76,914,294 (GRCm38)		probably benign	Het
Ttn	A	C	2: 76,907,736 (GRCm38)	V4153G	probably benign	Het
Unc80	A	G	1: 66,521,597 (GRCm38)	E828G	possibly damaging	Het
Vav3	A	G	3: 109,508,854 (GRCm38)	N263S	possibly damaging	Het
Vmn1r183	A	C	7: 24,055,557 (GRCm38)	T262P	possibly damaging	Het
Vmn2r67	G	A	7: 85,149,934 (GRCm38)	P522S	possibly damaging	Het
Vps35	T	A	8: 85,299,457 (GRCm38)	M1L	probably benign	Het
Wdr63	G	A	3: 146,066,893 (GRCm38)	S466L	probably benign	Het
Xpo7	A	T	14: 70,704,690 (GRCm38)	L79*	probably null	Het
Zc3h14	G	A	12: 98,759,828 (GRCm38)	R324H	probably damaging	Het
Zfp330	T	C	8: 82,772,984 (GRCm38)	T6A	probably damaging	Het
Zfp948	T	A	17: 21,587,024 (GRCm38)	H159Q	unknown	Het

Other mutations in Scn7a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00090:Scn7a	APN	2	66,683,327 (GRCm38)	splice site	probably benign
IGL00432:Scn7a	APN	2	66,741,982 (GRCm38)	nonsense	probably null
IGL00720:Scn7a	APN	2	66,676,044 (GRCm38)	missense	possibly damaging	0.67
IGL00783:Scn7a	APN	2	66,692,564 (GRCm38)	missense	probably damaging	0.99
IGL00784:Scn7a	APN	2	66,692,564 (GRCm38)	missense	probably damaging	0.99
IGL00926:Scn7a	APN	2	66,684,131 (GRCm38)	missense	probably benign	0.06
IGL00963:Scn7a	APN	2	66,703,945 (GRCm38)	splice site	probably benign
IGL01099:Scn7a	APN	2	66,684,238 (GRCm38)	missense	probably damaging	1.00
IGL01326:Scn7a	APN	2	66,752,260 (GRCm38)	missense	probably benign	0.13
IGL01538:Scn7a	APN	2	66,703,852 (GRCm38)	missense	probably benign
IGL01624:Scn7a	APN	2	66,751,925 (GRCm38)	missense	probably benign	0.07
IGL01794:Scn7a	APN	2	66,675,509 (GRCm38)	missense	probably benign
IGL02100:Scn7a	APN	2	66,675,499 (GRCm38)	makesense	probably null
IGL02326:Scn7a	APN	2	66,700,048 (GRCm38)	missense	probably benign	0.00
IGL02472:Scn7a	APN	2	66,752,314 (GRCm38)	missense	probably damaging	1.00
IGL02528:Scn7a	APN	2	66,700,175 (GRCm38)	missense	probably damaging	1.00
IGL02798:Scn7a	APN	2	66,713,875 (GRCm38)	missense	probably benign	0.00
IGL03026:Scn7a	APN	2	66,676,098 (GRCm38)	missense	probably damaging	0.99
IGL03071:Scn7a	APN	2	66,699,947 (GRCm38)	missense	possibly damaging	0.89
IGL03080:Scn7a	APN	2	66,697,816 (GRCm38)	missense	probably benign	0.01
IGL03180:Scn7a	APN	2	66,676,234 (GRCm38)	missense	possibly damaging	0.94
IGL03337:Scn7a	APN	2	66,675,960 (GRCm38)	missense	probably benign	0.00
alert	UTSW	2	66,680,246 (GRCm38)	nonsense	probably null
glimmer	UTSW	2	66,743,703 (GRCm38)	missense	probably damaging	0.96
Uptick	UTSW	2	66,700,049 (GRCm38)	nonsense	probably null
PIT4514001:Scn7a	UTSW	2	66,684,179 (GRCm38)	missense	probably damaging	1.00
R0004:Scn7a	UTSW	2	66,687,795 (GRCm38)	missense	possibly damaging	0.81
R0076:Scn7a	UTSW	2	66,714,037 (GRCm38)	missense	probably benign	0.04
R0230:Scn7a	UTSW	2	66,726,284 (GRCm38)	missense	probably damaging	1.00
R0463:Scn7a	UTSW	2	66,675,740 (GRCm38)	missense	probably benign	0.05
R0846:Scn7a	UTSW	2	66,697,600 (GRCm38)	missense	possibly damaging	0.71
R1237:Scn7a	UTSW	2	66,680,295 (GRCm38)	missense	probably damaging	0.98
R1282:Scn7a	UTSW	2	66,700,849 (GRCm38)	missense	probably damaging	0.98
R1467:Scn7a	UTSW	2	66,689,558 (GRCm38)	missense	probably benign	0.01
R1467:Scn7a	UTSW	2	66,689,558 (GRCm38)	missense	probably benign	0.01
R1501:Scn7a	UTSW	2	66,700,163 (GRCm38)	missense	probably benign	0.37
R1672:Scn7a	UTSW	2	66,697,600 (GRCm38)	missense	possibly damaging	0.71
R1690:Scn7a	UTSW	2	66,675,943 (GRCm38)	missense	probably damaging	0.99
R1712:Scn7a	UTSW	2	66,705,103 (GRCm38)	missense	probably benign	0.05
R1758:Scn7a	UTSW	2	66,700,887 (GRCm38)	missense	probably damaging	0.97
R1758:Scn7a	UTSW	2	66,680,183 (GRCm38)	missense	probably benign	0.00
R1775:Scn7a	UTSW	2	66,680,955 (GRCm38)	missense	probably benign	0.02
R1848:Scn7a	UTSW	2	66,684,013 (GRCm38)	critical splice donor site	probably null
R1851:Scn7a	UTSW	2	66,680,291 (GRCm38)	missense	probably benign
R1919:Scn7a	UTSW	2	66,699,973 (GRCm38)	missense	probably damaging	1.00
R1932:Scn7a	UTSW	2	66,676,102 (GRCm38)	missense	probably damaging	1.00
R1945:Scn7a	UTSW	2	66,675,980 (GRCm38)	missense	probably damaging	1.00
R1970:Scn7a	UTSW	2	66,684,289 (GRCm38)	missense	possibly damaging	0.89
R1998:Scn7a	UTSW	2	66,683,269 (GRCm38)	missense	probably damaging	0.99
R2008:Scn7a	UTSW	2	66,687,747 (GRCm38)	missense	possibly damaging	0.82
R2038:Scn7a	UTSW	2	66,737,436 (GRCm38)	missense	probably damaging	1.00
R2113:Scn7a	UTSW	2	66,675,968 (GRCm38)	missense	probably damaging	1.00
R2128:Scn7a	UTSW	2	66,697,986 (GRCm38)	missense	probably damaging	0.99
R2163:Scn7a	UTSW	2	66,675,956 (GRCm38)	missense	probably damaging	0.97
R2421:Scn7a	UTSW	2	66,726,302 (GRCm38)	splice site	probably benign
R2446:Scn7a	UTSW	2	66,692,658 (GRCm38)	missense	probably damaging	0.98
R2922:Scn7a	UTSW	2	66,700,207 (GRCm38)	splice site	probably benign
R3015:Scn7a	UTSW	2	66,699,896 (GRCm38)	missense	probably benign	0.08
R3034:Scn7a	UTSW	2	66,682,808 (GRCm38)	missense	probably damaging	1.00
R3419:Scn7a	UTSW	2	66,700,895 (GRCm38)	frame shift	probably null
R3429:Scn7a	UTSW	2	66,700,895 (GRCm38)	frame shift	probably null
R3430:Scn7a	UTSW	2	66,700,895 (GRCm38)	frame shift	probably null
R3434:Scn7a	UTSW	2	66,675,503 (GRCm38)	missense	probably benign	0.01
R3803:Scn7a	UTSW	2	66,680,246 (GRCm38)	nonsense	probably null
R3831:Scn7a	UTSW	2	66,697,684 (GRCm38)	missense	probably damaging	0.96
R3833:Scn7a	UTSW	2	66,697,684 (GRCm38)	missense	probably damaging	0.96
R4017:Scn7a	UTSW	2	66,741,985 (GRCm38)	missense	probably damaging	1.00
R4244:Scn7a	UTSW	2	66,742,001 (GRCm38)	missense	probably benign	0.00
R4245:Scn7a	UTSW	2	66,742,001 (GRCm38)	missense	probably benign	0.00
R4276:Scn7a	UTSW	2	66,684,063 (GRCm38)	missense	probably damaging	0.97
R4307:Scn7a	UTSW	2	66,675,755 (GRCm38)	missense	possibly damaging	0.47
R4327:Scn7a	UTSW	2	66,737,471 (GRCm38)	missense	probably damaging	1.00
R4353:Scn7a	UTSW	2	66,676,436 (GRCm38)	missense	probably benign	0.00
R4721:Scn7a	UTSW	2	66,684,185 (GRCm38)	missense	probably damaging	1.00
R4722:Scn7a	UTSW	2	66,700,884 (GRCm38)	missense	possibly damaging	0.95
R4781:Scn7a	UTSW	2	66,703,760 (GRCm38)	missense	possibly damaging	0.95
R4792:Scn7a	UTSW	2	66,726,248 (GRCm38)	missense	probably damaging	1.00
R5362:Scn7a	UTSW	2	66,699,998 (GRCm38)	missense	probably damaging	1.00
R5437:Scn7a	UTSW	2	66,676,346 (GRCm38)	missense	probably damaging	1.00
R5729:Scn7a	UTSW	2	66,741,957 (GRCm38)	critical splice donor site	probably null
R5777:Scn7a	UTSW	2	66,692,569 (GRCm38)	missense	probably damaging	1.00
R5785:Scn7a	UTSW	2	66,697,568 (GRCm38)	missense	possibly damaging	0.79
R5821:Scn7a	UTSW	2	66,743,703 (GRCm38)	missense	probably damaging	0.96
R5830:Scn7a	UTSW	2	66,714,051 (GRCm38)	nonsense	probably null
R5877:Scn7a	UTSW	2	66,699,873 (GRCm38)	nonsense	probably null
R5881:Scn7a	UTSW	2	66,675,526 (GRCm38)	missense	probably benign	0.01
R5967:Scn7a	UTSW	2	66,675,713 (GRCm38)	missense	probably damaging	1.00
R5988:Scn7a	UTSW	2	66,726,214 (GRCm38)	nonsense	probably null
R6077:Scn7a	UTSW	2	66,697,596 (GRCm38)	missense	probably damaging	1.00
R6135:Scn7a	UTSW	2	66,703,900 (GRCm38)	missense	probably benign
R6242:Scn7a	UTSW	2	66,700,766 (GRCm38)	missense	probably benign	0.00
R6264:Scn7a	UTSW	2	66,675,526 (GRCm38)	missense	possibly damaging	0.93
R6544:Scn7a	UTSW	2	66,684,100 (GRCm38)	missense	probably damaging	1.00
R6770:Scn7a	UTSW	2	66,729,184 (GRCm38)	splice site	probably null
R6997:Scn7a	UTSW	2	66,703,803 (GRCm38)	missense	probably damaging	1.00
R7014:Scn7a	UTSW	2	66,741,959 (GRCm38)	missense	probably null	1.00
R7126:Scn7a	UTSW	2	66,757,286 (GRCm38)	missense	possibly damaging	0.80
R7129:Scn7a	UTSW	2	66,700,193 (GRCm38)	missense	probably benign	0.14
R7176:Scn7a	UTSW	2	66,676,288 (GRCm38)	missense	probably damaging	1.00
R7185:Scn7a	UTSW	2	66,687,795 (GRCm38)	missense	possibly damaging	0.81
R7276:Scn7a	UTSW	2	66,757,162 (GRCm38)	missense	probably damaging	1.00
R7332:Scn7a	UTSW	2	66,692,554 (GRCm38)	nonsense	probably null
R7421:Scn7a	UTSW	2	66,675,532 (GRCm38)	missense	probably benign	0.07
R7488:Scn7a	UTSW	2	66,757,230 (GRCm38)	missense	probably benign	0.16
R7636:Scn7a	UTSW	2	66,743,828 (GRCm38)	missense	possibly damaging	0.67
R7685:Scn7a	UTSW	2	66,676,192 (GRCm38)	missense	probably damaging	1.00
R7711:Scn7a	UTSW	2	66,700,877 (GRCm38)	missense	probably damaging	1.00
R7813:Scn7a	UTSW	2	66,676,345 (GRCm38)	missense	probably damaging	1.00
R7833:Scn7a	UTSW	2	66,676,150 (GRCm38)	missense	probably damaging	1.00
R7914:Scn7a	UTSW	2	66,699,950 (GRCm38)	missense	probably damaging	0.97
R7953:Scn7a	UTSW	2	66,757,326 (GRCm38)	missense	possibly damaging	0.90
R7970:Scn7a	UTSW	2	66,675,829 (GRCm38)	missense	probably damaging	1.00
R8061:Scn7a	UTSW	2	66,692,594 (GRCm38)	missense	probably damaging	1.00
R8121:Scn7a	UTSW	2	66,700,859 (GRCm38)	missense	probably damaging	1.00
R8172:Scn7a	UTSW	2	66,675,847 (GRCm38)	missense	possibly damaging	0.90
R8209:Scn7a	UTSW	2	66,700,860 (GRCm38)	missense	possibly damaging	0.88
R8226:Scn7a	UTSW	2	66,700,860 (GRCm38)	missense	possibly damaging	0.88
R8288:Scn7a	UTSW	2	66,675,974 (GRCm38)	missense	probably damaging	1.00
R8431:Scn7a	UTSW	2	66,703,820 (GRCm38)	missense	possibly damaging	0.62
R8678:Scn7a	UTSW	2	66,743,697 (GRCm38)	splice site	probably benign
R8745:Scn7a	UTSW	2	66,680,182 (GRCm38)	missense	probably benign
R8781:Scn7a	UTSW	2	66,737,431 (GRCm38)	missense	probably benign	0.03
R8848:Scn7a	UTSW	2	66,700,049 (GRCm38)	nonsense	probably null
R8878:Scn7a	UTSW	2	66,675,855 (GRCm38)	missense	probably damaging	1.00
R8943:Scn7a	UTSW	2	66,694,862 (GRCm38)	synonymous	silent
R8991:Scn7a	UTSW	2	66,684,244 (GRCm38)	missense	possibly damaging	0.65
R9147:Scn7a	UTSW	2	66,684,163 (GRCm38)	missense	possibly damaging	0.89
R9148:Scn7a	UTSW	2	66,684,163 (GRCm38)	missense	possibly damaging	0.89
R9402:Scn7a	UTSW	2	66,680,112 (GRCm38)	missense	probably damaging	1.00
R9501:Scn7a	UTSW	2	66,752,235 (GRCm38)	missense	probably benign	0.00
R9546:Scn7a	UTSW	2	66,752,259 (GRCm38)	missense	possibly damaging	0.93
R9715:Scn7a	UTSW	2	66,689,558 (GRCm38)	missense	possibly damaging	0.93
X0060:Scn7a	UTSW	2	66,689,682 (GRCm38)	missense	probably benign	0.01
X0066:Scn7a	UTSW	2	66,680,192 (GRCm38)	missense	probably benign
Z1088:Scn7a	UTSW	2	66,713,951 (GRCm38)	missense	probably damaging	0.98
Z1177:Scn7a	UTSW	2	66,752,269 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGATTCGGAACACGGTCATG -3'
(R):5'- GTCCCAGTAAGTAGAATTCAAGACC -3'

Sequencing Primer
(F):5'- TTCGGAACACGGTCATGTAGGC -3'
(R):5'- CAAAGAGTGGATGTTTTTGCCCAC -3'

Posted On

2018-03-15