Incidental Mutation 'R6296:Supt6'
ID 508779
Institutional Source Beutler Lab
Gene Symbol Supt6
Ensembl Gene ENSMUSG00000002052
Gene Name SPT6, histone chaperone and transcription elongation factor
Synonyms SPT6, 5131400N11Rik, Supt6h
MMRRC Submission 044407-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R6296 (G1)
Quality Score 225.009
Status Not validated
Chromosome 11
Chromosomal Location 78097575-78136798 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 78116885 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Glutamine at position 589 (R589Q)
Ref Sequence ENSEMBL: ENSMUSP00000002121 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000002121]
AlphaFold Q62383
Predicted Effect possibly damaging
Transcript: ENSMUST00000002121
AA Change: R589Q

PolyPhen 2 Score 0.478 (Sensitivity: 0.89; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000002121
Gene: ENSMUSG00000002052
AA Change: R589Q

DomainStartEndE-ValueType
low complexity region 2 23 N/A INTRINSIC
Pfam:SPT6_acidic 37 127 8.8e-19 PFAM
low complexity region 146 164 N/A INTRINSIC
low complexity region 170 189 N/A INTRINSIC
low complexity region 192 203 N/A INTRINSIC
low complexity region 220 250 N/A INTRINSIC
low complexity region 252 267 N/A INTRINSIC
Pfam:HTH_44 305 432 1.3e-28 PFAM
low complexity region 494 509 N/A INTRINSIC
YqgFc 779 894 4.27e-21 SMART
Pfam:HHH_7 935 1038 3.1e-55 PFAM
Pfam:HHH_3 966 1036 5.2e-10 PFAM
Pfam:DLD 1051 1159 6.8e-39 PFAM
S1 1221 1282 2.8e-3 SMART
SH2 1332 1421 4.12e-11 SMART
low complexity region 1441 1454 N/A INTRINSIC
Blast:SH2 1455 1517 9e-19 BLAST
low complexity region 1586 1599 N/A INTRINSIC
low complexity region 1639 1664 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000108314
Meta Mutation Damage Score 0.1795 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.6%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mice homozygous for a knock-out allele exhibit lethality during pre-implantation development. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 82 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933427D14Rik T C 11: 72,086,580 (GRCm39) K277R probably damaging Het
Aatf T C 11: 84,363,926 (GRCm39) Y267C probably benign Het
Adcy5 A G 16: 35,124,080 (GRCm39) Y1253C probably damaging Het
Adgra3 G A 5: 50,118,189 (GRCm39) P1120S probably benign Het
Adm A G 7: 110,227,561 (GRCm39) T26A probably benign Het
Ahnak T G 19: 8,980,669 (GRCm39) V651G probably damaging Het
Akr7a5 G A 4: 139,045,532 (GRCm39) V358I probably benign Het
Ankar T G 1: 72,682,417 (GRCm39) T1383P probably damaging Het
Bpifb6 G C 2: 153,748,812 (GRCm39) K269N possibly damaging Het
Cacna1c T C 6: 118,575,684 (GRCm39) E1927G possibly damaging Het
Cacna1c T A 6: 118,629,675 (GRCm39) T1249S probably benign Het
Cacna1h G A 17: 25,602,053 (GRCm39) R1596C probably damaging Het
Cbll1 A G 12: 31,537,507 (GRCm39) V415A probably benign Het
Cd300lf C T 11: 115,015,195 (GRCm39) V132I probably benign Het
Cfap54 T C 10: 92,902,708 (GRCm39) Y148C probably damaging Het
Coa5 A G 1: 37,467,428 (GRCm39) S60P probably damaging Het
Col6a2 T C 10: 76,446,883 (GRCm39) N342D probably damaging Het
Cyp2c29 T G 19: 39,318,705 (GRCm39) I434S possibly damaging Het
Ddx11 G A 17: 66,457,724 (GRCm39) probably null Het
Dgke C T 11: 88,931,575 (GRCm39) V560I probably benign Het
Dusp16 C A 6: 134,697,456 (GRCm39) probably null Het
Enpp4 G T 17: 44,413,371 (GRCm39) N54K probably benign Het
Epn1 G A 7: 5,093,122 (GRCm39) A145T probably damaging Het
Erc2 A T 14: 27,802,112 (GRCm39) K764M probably damaging Het
Ercc6 G T 14: 32,248,360 (GRCm39) E304* probably null Het
Fam117a T A 11: 95,254,971 (GRCm39) C115S possibly damaging Het
Galntl5 T C 5: 25,391,163 (GRCm39) S21P probably benign Het
Gm6358 T C 16: 88,937,970 (GRCm39) S70P unknown Het
Grip1 C T 10: 119,911,369 (GRCm39) Q696* probably null Het
Haus5 C T 7: 30,358,401 (GRCm39) W298* probably null Het
Hsfy2 T A 1: 56,676,351 (GRCm39) H62L probably benign Het
Ighm T C 12: 113,385,187 (GRCm39) I258V unknown Het
Igkv13-71-1 G T 6: 69,212,783 (GRCm39) noncoding transcript Het
Inava C T 1: 136,148,809 (GRCm39) probably null Het
Irx1 A C 13: 72,107,787 (GRCm39) S298R probably damaging Het
Jarid2 T A 13: 45,056,539 (GRCm39) Y443N possibly damaging Het
Kcnc1 G A 7: 46,084,740 (GRCm39) A555T probably benign Het
Krtap4-6 T A 11: 99,556,245 (GRCm39) R161* probably null Het
Lipo3 T C 19: 33,757,737 (GRCm39) D244G probably benign Het
Lmo2 T G 2: 103,800,946 (GRCm39) V39G possibly damaging Het
Lrch2 C T X: 146,263,553 (GRCm39) A369T probably damaging Homo
Macf1 A T 4: 123,326,668 (GRCm39) I4943N probably damaging Het
Mkx A T 18: 7,000,591 (GRCm39) probably null Het
Nek1 C T 8: 61,525,343 (GRCm39) Q594* probably null Het
Nipbl T C 15: 8,330,379 (GRCm39) M2349V possibly damaging Het
Nup155 T A 15: 8,182,639 (GRCm39) C1201S probably damaging Het
Or2y8 C A 11: 52,035,423 (GRCm39) R311S probably benign Het
Or4a76 G A 2: 89,460,975 (GRCm39) T89I probably damaging Het
Or5ac25 T C 16: 59,181,769 (GRCm39) K271E probably benign Het
Osbpl1a A G 18: 12,952,560 (GRCm39) probably null Het
Pbx1 T C 1: 168,011,184 (GRCm39) D372G possibly damaging Het
Pikfyve T A 1: 65,302,112 (GRCm39) S1668T probably damaging Het
Pitpnc1 T C 11: 107,117,092 (GRCm39) H193R probably damaging Het
Plag1 G T 4: 3,904,499 (GRCm39) H231N probably damaging Het
Prmt8 A G 6: 127,688,767 (GRCm39) I201T probably damaging Het
Ptpn23 A T 9: 110,222,894 (GRCm39) N54K probably damaging Het
Rab11fip4 T C 11: 79,581,655 (GRCm39) probably null Het
Rassf9 T A 10: 102,381,614 (GRCm39) I332N probably damaging Het
Rgs9 T C 11: 109,159,813 (GRCm39) N173S probably benign Het
Rhbdl1 A G 17: 26,053,943 (GRCm39) L309P probably damaging Het
Rnf214 A G 9: 45,779,119 (GRCm39) S389P probably benign Het
Rxfp1 A C 3: 79,575,155 (GRCm39) L181R probably damaging Het
Sfxn1 C T 13: 54,247,899 (GRCm39) T208I probably benign Het
Sgo2b C T 8: 64,380,827 (GRCm39) M668I probably benign Het
Sh3bp4 C A 1: 89,073,211 (GRCm39) S686R probably damaging Het
Spata31d1d G A 13: 59,876,278 (GRCm39) T419I possibly damaging Het
Spata31d1e T C 13: 59,890,497 (GRCm39) D441G probably benign Het
Spink5 T C 18: 44,147,824 (GRCm39) S857P probably damaging Het
Stard13 A T 5: 150,986,138 (GRCm39) S339R probably damaging Het
Stk35 T A 2: 129,652,808 (GRCm39) Y436* probably null Het
Tinag T A 9: 76,904,217 (GRCm39) E402V possibly damaging Het
Tmem183a T C 1: 134,289,349 (GRCm39) D27G probably damaging Het
Tnfrsf13c T C 15: 82,108,103 (GRCm39) T56A probably damaging Het
Tnrc18 A C 5: 142,719,331 (GRCm39) L1985R unknown Het
Ttn G A 2: 76,579,673 (GRCm39) T23740M probably damaging Het
Ufl1 G T 4: 25,270,572 (GRCm39) Q215K probably benign Het
Unkl T C 17: 25,450,839 (GRCm39) *232R probably null Het
Wnk4 A T 11: 101,164,824 (GRCm39) N718Y probably damaging Het
Xkr4 T C 1: 3,286,793 (GRCm39) T466A probably benign Het
Zfp451 T A 1: 33,808,898 (GRCm39) K988* probably null Het
Zfp503 G C 14: 22,035,868 (GRCm39) Y349* probably null Het
Zfp990 T A 4: 145,264,673 (GRCm39) F557Y possibly damaging Het
Other mutations in Supt6
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00911:Supt6 APN 11 78,122,007 (GRCm39) missense possibly damaging 0.94
IGL01457:Supt6 APN 11 78,111,969 (GRCm39) missense probably damaging 1.00
IGL01608:Supt6 APN 11 78,116,309 (GRCm39) missense probably damaging 1.00
IGL01739:Supt6 APN 11 78,113,013 (GRCm39) missense probably damaging 1.00
IGL01765:Supt6 APN 11 78,112,985 (GRCm39) missense probably benign 0.09
IGL01894:Supt6 APN 11 78,113,664 (GRCm39) missense probably benign 0.00
IGL01952:Supt6 APN 11 78,116,586 (GRCm39) missense probably benign 0.01
IGL02067:Supt6 APN 11 78,121,983 (GRCm39) missense probably benign 0.01
IGL02244:Supt6 APN 11 78,123,623 (GRCm39) missense possibly damaging 0.92
IGL02267:Supt6 APN 11 78,117,030 (GRCm39) missense possibly damaging 0.72
IGL02379:Supt6 APN 11 78,116,195 (GRCm39) missense possibly damaging 0.75
IGL02541:Supt6 APN 11 78,117,744 (GRCm39) missense probably damaging 0.99
IGL02635:Supt6 APN 11 78,103,565 (GRCm39) missense probably damaging 1.00
IGL03347:Supt6 APN 11 78,123,011 (GRCm39) missense possibly damaging 0.71
IGL02980:Supt6 UTSW 11 78,116,548 (GRCm39) missense probably damaging 1.00
IGL02991:Supt6 UTSW 11 78,116,179 (GRCm39) missense probably damaging 1.00
R0145:Supt6 UTSW 11 78,099,062 (GRCm39) missense probably benign 0.22
R0371:Supt6 UTSW 11 78,113,983 (GRCm39) missense probably benign 0.00
R0452:Supt6 UTSW 11 78,117,829 (GRCm39) missense probably damaging 1.00
R0464:Supt6 UTSW 11 78,107,164 (GRCm39) missense probably benign 0.33
R0616:Supt6 UTSW 11 78,100,321 (GRCm39) missense probably damaging 1.00
R0653:Supt6 UTSW 11 78,116,841 (GRCm39) missense probably benign 0.01
R0788:Supt6 UTSW 11 78,098,598 (GRCm39) unclassified probably benign
R1103:Supt6 UTSW 11 78,116,299 (GRCm39) missense possibly damaging 0.59
R1282:Supt6 UTSW 11 78,119,594 (GRCm39) missense possibly damaging 0.83
R1460:Supt6 UTSW 11 78,113,024 (GRCm39) missense possibly damaging 0.93
R1508:Supt6 UTSW 11 78,107,029 (GRCm39) critical splice donor site probably null
R1850:Supt6 UTSW 11 78,110,703 (GRCm39) splice site probably benign
R1854:Supt6 UTSW 11 78,123,366 (GRCm39) missense possibly damaging 0.51
R1855:Supt6 UTSW 11 78,123,366 (GRCm39) missense possibly damaging 0.51
R2054:Supt6 UTSW 11 78,115,187 (GRCm39) splice site probably benign
R2098:Supt6 UTSW 11 78,104,087 (GRCm39) splice site probably null
R2146:Supt6 UTSW 11 78,121,758 (GRCm39) missense probably damaging 1.00
R2167:Supt6 UTSW 11 78,098,993 (GRCm39) missense possibly damaging 0.94
R4621:Supt6 UTSW 11 78,103,572 (GRCm39) missense possibly damaging 0.65
R4734:Supt6 UTSW 11 78,115,509 (GRCm39) missense probably benign 0.01
R4825:Supt6 UTSW 11 78,098,960 (GRCm39) missense possibly damaging 0.84
R5575:Supt6 UTSW 11 78,119,787 (GRCm39) missense probably damaging 1.00
R5789:Supt6 UTSW 11 78,124,412 (GRCm39) missense unknown
R5889:Supt6 UTSW 11 78,103,574 (GRCm39) missense probably damaging 0.98
R6297:Supt6 UTSW 11 78,116,885 (GRCm39) missense possibly damaging 0.48
R6394:Supt6 UTSW 11 78,121,891 (GRCm39) missense probably damaging 1.00
R6702:Supt6 UTSW 11 78,122,626 (GRCm39) missense possibly damaging 0.93
R6737:Supt6 UTSW 11 78,122,644 (GRCm39) missense probably damaging 0.99
R6751:Supt6 UTSW 11 78,099,775 (GRCm39) missense probably benign 0.09
R6853:Supt6 UTSW 11 78,123,656 (GRCm39) missense possibly damaging 0.85
R7213:Supt6 UTSW 11 78,122,976 (GRCm39) missense probably damaging 1.00
R7259:Supt6 UTSW 11 78,098,442 (GRCm39) missense probably damaging 0.99
R7609:Supt6 UTSW 11 78,117,777 (GRCm39) missense probably benign 0.01
R7776:Supt6 UTSW 11 78,100,355 (GRCm39) missense probably damaging 0.99
R8683:Supt6 UTSW 11 78,108,727 (GRCm39) missense probably benign 0.13
R8895:Supt6 UTSW 11 78,103,664 (GRCm39) missense probably damaging 0.98
R9097:Supt6 UTSW 11 78,113,100 (GRCm39) missense probably benign 0.00
R9175:Supt6 UTSW 11 78,112,052 (GRCm39) missense possibly damaging 0.70
R9228:Supt6 UTSW 11 78,116,612 (GRCm39) missense probably benign 0.03
R9311:Supt6 UTSW 11 78,116,284 (GRCm39) missense probably damaging 1.00
R9476:Supt6 UTSW 11 78,120,290 (GRCm39) missense probably damaging 1.00
R9510:Supt6 UTSW 11 78,120,290 (GRCm39) missense probably damaging 1.00
R9748:Supt6 UTSW 11 78,108,767 (GRCm39) missense probably damaging 0.96
X0067:Supt6 UTSW 11 78,123,501 (GRCm39) missense probably benign
Z1176:Supt6 UTSW 11 78,102,662 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCTCCACATAGATGCAGTTTAGTG -3'
(R):5'- CTCACACCTGAGCAGTTTGG -3'

Sequencing Primer
(F):5'- CACATAGATGCAGTTTAGTGTGGCG -3'
(R):5'- CTGAGCAGTTTGGGGAGAACCTC -3'
Posted On 2018-04-02