Mutagenetix > Incidental Mutation

Incidental Mutation 'R6298:Herc2'

508896

Institutional Source

Beutler Lab

Gene Symbol

Herc2

Ensembl Gene

ENSMUSG00000030451

Gene Name

HECT and RLD domain containing E3 ubiquitin protein ligase 2

Synonyms

D7H15F37S1, D7H15F32S1, rjs, jdf2, D15F32S1h

MMRRC Submission

044408-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.947) question?

Stock #

R6298 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

56050196-56231800 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 56191265 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 3444 (M3444T)

Ref Sequence

ENSEMBL: ENSMUSP00000145997 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076226] [ENSMUST00000164095] [ENSMUST00000205303]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000076226
AA Change: M3444T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000075579
Gene: ENSMUSG00000030451
AA Change: M3444T

Domain	Start	End	E-Value	Type
low complexity region	73	87	N/A	INTRINSIC
low complexity region	164	175	N/A	INTRINSIC
low complexity region	198	212	N/A	INTRINSIC
low complexity region	272	281	N/A	INTRINSIC
low complexity region	304	317	N/A	INTRINSIC
Pfam:RCC1	514	567	7.6e-16	PFAM
Pfam:RCC1_2	554	583	6e-9	PFAM
Pfam:RCC1	570	615	7.1e-17	PFAM
Pfam:RCC1_2	606	637	6.9e-8	PFAM
Pfam:RCC1	624	673	7.2e-15	PFAM
Pfam:RCC1	676	725	3.3e-18	PFAM
Pfam:RCC1_2	712	740	1.6e-9	PFAM
low complexity region	854	866	N/A	INTRINSIC
low complexity region	902	913	N/A	INTRINSIC
coiled coil region	950	977	N/A	INTRINSIC
low complexity region	1052	1066	N/A	INTRINSIC
Cyt-b5	1211	1284	1.08e-1	SMART
low complexity region	1310	1316	N/A	INTRINSIC
low complexity region	1440	1446	N/A	INTRINSIC
low complexity region	1545	1563	N/A	INTRINSIC
coiled coil region	1651	1674	N/A	INTRINSIC
Pfam:MIB_HERC2	1871	1933	5.8e-29	PFAM
low complexity region	1939	1952	N/A	INTRINSIC
low complexity region	2211	2222	N/A	INTRINSIC
low complexity region	2381	2388	N/A	INTRINSIC
low complexity region	2402	2416	N/A	INTRINSIC
low complexity region	2521	2540	N/A	INTRINSIC
Pfam:Cul7	2555	2633	2.6e-43	PFAM
ZnF_ZZ	2703	2747	5.39e-11	SMART
APC10	2780	2933	5.1e-41	SMART
TECPR	2978	3019	7.59e0	SMART
Pfam:RCC1_2	3048	3079	9.2e-8	PFAM
Pfam:RCC1	3066	3115	3.7e-17	PFAM
Pfam:RCC1_2	3102	3131	3.9e-11	PFAM
Pfam:RCC1	3118	3162	1.9e-15	PFAM
Pfam:RCC1	3172	3221	9.6e-15	PFAM
Pfam:RCC1_2	3208	3236	2.2e-7	PFAM
TECPR	3241	3284	1.32e2	SMART
low complexity region	3357	3365	N/A	INTRINSIC
low complexity region	3430	3447	N/A	INTRINSIC
low complexity region	3480	3495	N/A	INTRINSIC
low complexity region	3755	3771	N/A	INTRINSIC
TECPR	3972	4012	2.41e1	SMART
Pfam:RCC1_2	4041	4072	5.1e-8	PFAM
Pfam:RCC1	4059	4108	1.5e-16	PFAM
Pfam:RCC1	4111	4155	7.9e-16	PFAM
TECPR	4184	4225	8.42e1	SMART
Pfam:RCC1_2	4253	4282	4.3e-10	PFAM
Pfam:RCC1	4269	4318	1.2e-17	PFAM
Blast:HECTc	4340	4425	6e-18	BLAST
HECTc	4455	4801	1.37e-62	SMART
low complexity region	4808	4828	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000164095
AA Change: M3444T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000131573
Gene: ENSMUSG00000030451
AA Change: M3444T

Domain	Start	End	E-Value	Type
low complexity region	73	87	N/A	INTRINSIC
low complexity region	164	175	N/A	INTRINSIC
low complexity region	198	212	N/A	INTRINSIC
low complexity region	272	281	N/A	INTRINSIC
low complexity region	304	317	N/A	INTRINSIC
Pfam:RCC1	514	567	2.9e-15	PFAM
Pfam:RCC1_2	554	583	1.6e-8	PFAM
Pfam:RCC1	570	615	2.3e-16	PFAM
Pfam:RCC1	624	673	1.2e-14	PFAM
Pfam:RCC1_2	660	689	2.1e-7	PFAM
Pfam:RCC1	676	725	9.8e-18	PFAM
Pfam:RCC1_2	712	740	1.1e-9	PFAM
low complexity region	854	866	N/A	INTRINSIC
low complexity region	902	913	N/A	INTRINSIC
coiled coil region	950	977	N/A	INTRINSIC
low complexity region	1052	1066	N/A	INTRINSIC
Cyt-b5	1211	1284	1.08e-1	SMART
low complexity region	1310	1316	N/A	INTRINSIC
low complexity region	1440	1446	N/A	INTRINSIC
low complexity region	1545	1563	N/A	INTRINSIC
coiled coil region	1651	1674	N/A	INTRINSIC
Pfam:MIB_HERC2	1871	1931	9.3e-25	PFAM
low complexity region	1939	1952	N/A	INTRINSIC
low complexity region	2211	2222	N/A	INTRINSIC
low complexity region	2381	2388	N/A	INTRINSIC
low complexity region	2402	2416	N/A	INTRINSIC
low complexity region	2521	2540	N/A	INTRINSIC
Pfam:Cul7	2555	2632	1.4e-39	PFAM
ZnF_ZZ	2703	2747	5.39e-11	SMART
APC10	2780	2933	5.1e-41	SMART
TECPR	2978	3019	7.59e0	SMART
Pfam:RCC1_2	3048	3079	1.6e-7	PFAM
Pfam:RCC1	3066	3115	7.1e-16	PFAM
Pfam:RCC1_2	3102	3131	7.1e-11	PFAM
Pfam:RCC1	3118	3163	1.2e-14	PFAM
Pfam:RCC1	3172	3221	4.7e-15	PFAM
TECPR	3241	3284	1.32e2	SMART
low complexity region	3357	3365	N/A	INTRINSIC
low complexity region	3430	3447	N/A	INTRINSIC
low complexity region	3480	3495	N/A	INTRINSIC
low complexity region	3755	3771	N/A	INTRINSIC
TECPR	3972	4012	2.41e1	SMART
Pfam:RCC1_2	4041	4072	1.2e-7	PFAM
Pfam:RCC1	4059	4108	9.6e-15	PFAM
Pfam:RCC1	4111	4156	5.6e-15	PFAM
TECPR	4184	4225	8.42e1	SMART
Pfam:RCC1_2	4253	4282	7.3e-10	PFAM
Pfam:RCC1	4269	4318	1.6e-16	PFAM
Blast:HECTc	4340	4425	6e-18	BLAST
HECTc	4455	4801	1.37e-62	SMART
low complexity region	4808	4828	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000205303
AA Change: M3444T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

98% (79/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16. [provided by RefSeq, Mar 2012]
PHENOTYPE: Homozygotes for null mutations exhibit runting, nervousness, and incoordination. Males are sterile with sperm abnormalities, while females show reduced fertility and impaired maternal ability. Also see alleles at the Oca2 (p) locus for deletions that encompass the Herc2 gene. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abtb2	A	T	2: 103,709,488 (GRCm38)	M733L	probably benign	Het
Acss3	G	A	10: 107,084,856 (GRCm38)	P131L	probably damaging	Het
Adgrv1	T	A	13: 81,391,767 (GRCm38)	I5678F	probably benign	Het
Aff1	C	T	5: 103,754,720 (GRCm38)	L6F	possibly damaging	Het
Ank3	G	T	10: 69,850,176 (GRCm38)	R273L	probably damaging	Het
Anks1b	G	A	10: 90,680,837 (GRCm38)	G898D	probably damaging	Het
Anxa11	C	T	14: 25,872,734 (GRCm38)	P131S	unknown	Het
Ap4e1	T	A	2: 127,047,115 (GRCm38)	M500K	probably benign	Het
Bag3	T	C	7: 128,540,198 (GRCm38)	S138P	probably damaging	Het
Capn9	T	C	8: 124,617,454 (GRCm38)	V670A	probably benign	Het
Ccne2	T	A	4: 11,199,306 (GRCm38)	W236R	probably damaging	Het
Cdh23	A	T	10: 60,426,672 (GRCm38)	Y702*	probably null	Het
Chd1l	G	A	3: 97,587,167 (GRCm38)	A399V	probably damaging	Het
Cit	A	T	5: 115,948,065 (GRCm38)	E896V	probably damaging	Het
Cntln	T	A	4: 85,096,761 (GRCm38)	N1096K	probably damaging	Het
Cntnap5c	T	C	17: 58,104,752 (GRCm38)	C544R	probably damaging	Het
Csf1	A	T	3: 107,748,359 (GRCm38)	L452Q	possibly damaging	Het
Cts8	T	A	13: 61,249,223 (GRCm38)	K294N	possibly damaging	Het
Dcakd	T	G	11: 102,999,792 (GRCm38)	E56D	possibly damaging	Het
Dnah17	T	C	11: 118,108,161 (GRCm38)	I929V	probably benign	Het
Dnah2	G	T	11: 69,491,641 (GRCm38)	H1214Q	probably benign	Het
Dock9	T	C	14: 121,634,594 (GRCm38)	D536G	probably damaging	Het
Drc3	A	G	11: 60,393,770 (GRCm38)	N467S	possibly damaging	Het
Dysf	A	G	6: 84,107,136 (GRCm38)		probably null	Het
Ecpas	T	G	4: 58,877,157 (GRCm38)	T93P	probably damaging	Het
Evpl	A	T	11: 116,230,922 (GRCm38)	L378Q	probably damaging	Het
Fer1l4	A	T	2: 156,024,740 (GRCm38)	H1520Q	probably damaging	Het
Fhod1	A	T	8: 105,337,148 (GRCm38)		probably benign	Het
Gabra1	T	A	11: 42,182,378 (GRCm38)		probably benign	Het
Gm10549	C	A	18: 33,464,305 (GRCm38)		probably benign	Het
Gm14137	A	G	2: 119,175,091 (GRCm38)	T44A	possibly damaging	Het
H2bl1	T	A	13: 98,984,466 (GRCm38)	R17S	probably benign	Het
Igsf5	T	C	16: 96,396,448 (GRCm38)	S208P	possibly damaging	Het
Ino80b	A	G	6: 83,125,085 (GRCm38)	L12P	possibly damaging	Het
Insrr	A	G	3: 87,812,965 (GRCm38)	D970G	probably damaging	Het
Iqcf4	G	A	9: 106,568,675 (GRCm38)	A91V	probably benign	Het
Itga10	A	G	3: 96,656,762 (GRCm38)	T911A	probably benign	Het
Katnip	T	C	7: 125,870,697 (GRCm38)	V1446A	probably benign	Het
Klhl30	A	T	1: 91,357,364 (GRCm38)	D314V	probably benign	Het
Lpcat1	T	C	13: 73,510,955 (GRCm38)	V330A	possibly damaging	Het
Myef2l	T	C	3: 10,089,179 (GRCm38)	I336T	probably damaging	Het
Nhlrc3	A	T	3: 53,452,523 (GRCm38)	D306E	possibly damaging	Het
Notum	T	A	11: 120,657,940 (GRCm38)	I187F	probably damaging	Het
Nphp3	T	C	9: 104,015,441 (GRCm38)	L288P	probably damaging	Het
Ntrk2	G	T	13: 58,871,756 (GRCm38)	E394*	probably null	Het
Or6k2	T	A	1: 174,152,182 (GRCm38)	V222D	probably benign	Het
Pbld2	A	G	10: 63,039,152 (GRCm38)	K63E	probably benign	Het
Phc2	T	C	4: 128,748,189 (GRCm38)	M768T	possibly damaging	Het
Pik3c2g	G	A	6: 139,626,563 (GRCm38)	C249Y	probably damaging	Het
Plod1	G	A	4: 147,916,315 (GRCm38)		probably benign	Het
Plscr2	A	T	9: 92,290,719 (GRCm38)	T9S	probably benign	Het
Pnrc1	T	A	4: 33,246,315 (GRCm38)	M215L	probably benign	Het
Prcp	G	T	7: 92,928,633 (GRCm38)	C370F	probably damaging	Het
Pter	A	G	2: 12,978,394 (GRCm38)	N70S	probably damaging	Het
Ptprt	G	T	2: 161,553,859 (GRCm38)	H1131Q	probably damaging	Het
Rasal2	T	C	1: 157,411,862 (GRCm38)	D8G	possibly damaging	Het
Rcn2	A	C	9: 56,052,925 (GRCm38)	K159Q	probably benign	Het
Rex2	T	A	4: 147,057,515 (GRCm38)	C153*	probably null	Het
Rps6ka2	T	C	17: 7,170,367 (GRCm38)	F8S	possibly damaging	Het
Samd9l	A	G	6: 3,375,383 (GRCm38)	L626S	probably damaging	Het
Sptb	A	G	12: 76,620,654 (GRCm38)		probably null	Het
Srgap3	A	T	6: 112,816,610 (GRCm38)	V135D	probably damaging	Het
Srsf7	T	C	17: 80,207,253 (GRCm38)		probably benign	Het
Tacc2	A	G	7: 130,626,525 (GRCm38)	T1647A	probably benign	Het
Thap12	G	T	7: 98,703,405 (GRCm38)	A6S	probably damaging	Het
Tmem33	T	A	5: 67,268,551 (GRCm38)	L146*	probably null	Het
Trip13	C	A	13: 73,936,259 (GRCm38)	E36*	probably null	Het
Vkorc1l1	T	A	5: 129,942,238 (GRCm38)	C23S	probably damaging	Het
Vmn1r20	G	A	6: 57,432,127 (GRCm38)	R146H	probably benign	Het
Vmn1r222	T	A	13: 23,232,795 (GRCm38)	I83F	probably benign	Het
Vmn2r107	A	T	17: 20,355,782 (GRCm38)	I125F	probably benign	Het
Vmn2r116	A	G	17: 23,386,762 (GRCm38)	D216G	probably damaging	Het
Vwa3a	C	T	7: 120,795,651 (GRCm38)	T898I	probably benign	Het
Wdfy3	T	C	5: 101,968,946 (GRCm38)	D76G	probably damaging	Het
Wdhd1	T	C	14: 47,273,122 (GRCm38)	D148G	possibly damaging	Het
Xylt1	T	C	7: 117,656,737 (GRCm38)	I844T	probably damaging	Het
Zfp106	A	T	2: 120,522,704 (GRCm38)	V1535D	probably damaging	Het
Zfp385b	A	T	2: 77,413,979 (GRCm38)	L315Q	possibly damaging	Het
Zfp458	T	A	13: 67,256,806 (GRCm38)	H523L	probably damaging	Het
Zfp712	T	C	13: 67,041,329 (GRCm38)	H378R	probably damaging	Het
Zic1	T	C	9: 91,364,503 (GRCm38)	Y172C	probably damaging	Het

Other mutations in Herc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00329:Herc2	APN	7	56,124,299 (GRCm38)	missense	probably damaging	1.00
IGL00529:Herc2	APN	7	56,157,753 (GRCm38)	missense	probably benign
IGL00548:Herc2	APN	7	56,206,565 (GRCm38)	missense	probably benign	0.20
IGL00970:Herc2	APN	7	56,181,064 (GRCm38)	splice site	probably benign
IGL01141:Herc2	APN	7	56,212,841 (GRCm38)	missense	possibly damaging	0.47
IGL01147:Herc2	APN	7	56,156,949 (GRCm38)	missense	probably benign	0.43
IGL01150:Herc2	APN	7	56,181,133 (GRCm38)	missense	probably damaging	1.00
IGL01519:Herc2	APN	7	56,103,950 (GRCm38)	missense	probably damaging	1.00
IGL01576:Herc2	APN	7	56,226,661 (GRCm38)	critical splice donor site	probably null
IGL01626:Herc2	APN	7	56,085,142 (GRCm38)	missense	probably benign	0.02
IGL01658:Herc2	APN	7	56,159,452 (GRCm38)	missense	probably damaging	1.00
IGL01707:Herc2	APN	7	56,165,187 (GRCm38)	missense	probably damaging	1.00
IGL01727:Herc2	APN	7	56,137,806 (GRCm38)	missense	probably damaging	1.00
IGL01935:Herc2	APN	7	56,153,793 (GRCm38)	missense	probably benign
IGL01969:Herc2	APN	7	56,185,831 (GRCm38)	splice site	probably benign
IGL02074:Herc2	APN	7	56,087,444 (GRCm38)	splice site	probably benign
IGL02261:Herc2	APN	7	56,206,744 (GRCm38)	missense	probably damaging	0.99
IGL02339:Herc2	APN	7	56,121,722 (GRCm38)	missense	probably benign	0.01
IGL02353:Herc2	APN	7	56,114,812 (GRCm38)	missense	probably damaging	1.00
IGL02360:Herc2	APN	7	56,114,812 (GRCm38)	missense	probably damaging	1.00
IGL02409:Herc2	APN	7	56,220,469 (GRCm38)	splice site	probably null
IGL02528:Herc2	APN	7	56,108,893 (GRCm38)	splice site	probably benign
IGL02571:Herc2	APN	7	56,153,386 (GRCm38)	missense	probably damaging	1.00
IGL02578:Herc2	APN	7	56,106,535 (GRCm38)	splice site	probably null
IGL02661:Herc2	APN	7	56,113,073 (GRCm38)	missense	probably damaging	1.00
IGL02664:Herc2	APN	7	56,135,678 (GRCm38)	nonsense	probably null
IGL02675:Herc2	APN	7	56,164,101 (GRCm38)	missense	probably damaging	0.99
IGL02689:Herc2	APN	7	56,165,283 (GRCm38)	splice site	probably benign
IGL02710:Herc2	APN	7	56,137,814 (GRCm38)	missense	possibly damaging	0.95
IGL02750:Herc2	APN	7	56,204,379 (GRCm38)	splice site	probably benign
IGL02754:Herc2	APN	7	56,097,498 (GRCm38)	missense	probably damaging	1.00
IGL03029:Herc2	APN	7	56,168,967 (GRCm38)	missense	probably damaging	1.00
IGL03039:Herc2	APN	7	56,169,021 (GRCm38)	splice site	probably benign
IGL03082:Herc2	APN	7	56,185,923 (GRCm38)	missense	probably benign	0.19
IGL03090:Herc2	APN	7	56,204,473 (GRCm38)	missense	probably damaging	0.96
IGL03154:Herc2	APN	7	56,202,159 (GRCm38)	missense	probably damaging	1.00
IGL03165:Herc2	APN	7	56,191,912 (GRCm38)	missense	probably damaging	1.00
IGL03201:Herc2	APN	7	56,219,768 (GRCm38)	missense	probably damaging	1.00
IGL03234:Herc2	APN	7	56,103,862 (GRCm38)	missense	probably damaging	1.00
IGL03293:Herc2	APN	7	56,155,130 (GRCm38)	missense	probably benign	0.43
IGL03331:Herc2	APN	7	56,135,267 (GRCm38)	splice site	probably benign
IGL03340:Herc2	APN	7	56,090,920 (GRCm38)	missense	possibly damaging	0.51
IGL03409:Herc2	APN	7	56,228,569 (GRCm38)	missense	probably damaging	1.00
alarmed	UTSW	7	56,229,662 (GRCm38)	missense	possibly damaging	0.92
hyper	UTSW	7	56,159,417 (GRCm38)	missense	probably damaging	1.00
R0798_herc2_487	UTSW	7	56,135,683 (GRCm38)	critical splice donor site	probably null
R1370_Herc2_948	UTSW	7	56,168,873 (GRCm38)	missense	probably benign	0.01
R2030_Herc2_144	UTSW	7	56,184,373 (GRCm38)	missense	probably damaging	0.99
uptight	UTSW	7	56,113,210 (GRCm38)	missense	probably damaging	1.00
I0000:Herc2	UTSW	7	56,136,729 (GRCm38)	splice site	probably benign
PIT1430001:Herc2	UTSW	7	56,226,954 (GRCm38)	missense	probably damaging	1.00
R0009:Herc2	UTSW	7	56,207,812 (GRCm38)	missense	probably benign	0.03
R0009:Herc2	UTSW	7	56,207,812 (GRCm38)	missense	probably benign	0.03
R0058:Herc2	UTSW	7	56,170,483 (GRCm38)	missense	possibly damaging	0.93
R0114:Herc2	UTSW	7	56,153,774 (GRCm38)	splice site	probably benign
R0117:Herc2	UTSW	7	56,213,611 (GRCm38)	splice site	probably benign
R0141:Herc2	UTSW	7	56,121,561 (GRCm38)	missense	probably benign	0.17
R0266:Herc2	UTSW	7	56,206,578 (GRCm38)	missense	probably damaging	1.00
R0401:Herc2	UTSW	7	56,157,732 (GRCm38)	missense	probably damaging	0.99
R0403:Herc2	UTSW	7	56,159,417 (GRCm38)	missense	probably damaging	1.00
R0437:Herc2	UTSW	7	56,219,815 (GRCm38)	nonsense	probably null
R0491:Herc2	UTSW	7	56,122,366 (GRCm38)	missense	possibly damaging	0.54
R0499:Herc2	UTSW	7	56,184,369 (GRCm38)	nonsense	probably null
R0580:Herc2	UTSW	7	56,138,791 (GRCm38)	missense	probably damaging	1.00
R0650:Herc2	UTSW	7	56,113,210 (GRCm38)	missense	probably damaging	1.00
R0744:Herc2	UTSW	7	56,206,036 (GRCm38)	splice site	probably benign
R0798:Herc2	UTSW	7	56,135,683 (GRCm38)	critical splice donor site	probably null
R0842:Herc2	UTSW	7	56,121,705 (GRCm38)	missense	probably benign
R0849:Herc2	UTSW	7	56,206,578 (GRCm38)	missense	probably damaging	1.00
R0850:Herc2	UTSW	7	56,204,483 (GRCm38)	missense	probably benign	0.09
R0926:Herc2	UTSW	7	56,132,548 (GRCm38)	missense	possibly damaging	0.67
R1146:Herc2	UTSW	7	56,146,696 (GRCm38)	missense	probably benign
R1146:Herc2	UTSW	7	56,146,696 (GRCm38)	missense	probably benign
R1292:Herc2	UTSW	7	56,197,203 (GRCm38)	missense	probably benign	0.05
R1370:Herc2	UTSW	7	56,168,873 (GRCm38)	missense	probably benign	0.01
R1443:Herc2	UTSW	7	56,204,733 (GRCm38)	missense	possibly damaging	0.69
R1445:Herc2	UTSW	7	56,168,996 (GRCm38)	missense	probably damaging	1.00
R1541:Herc2	UTSW	7	56,135,657 (GRCm38)	missense	probably damaging	1.00
R1550:Herc2	UTSW	7	56,135,658 (GRCm38)	missense	probably damaging	1.00
R1551:Herc2	UTSW	7	56,146,669 (GRCm38)	missense	probably benign	0.01
R1633:Herc2	UTSW	7	56,229,369 (GRCm38)	missense	probably null	1.00
R1635:Herc2	UTSW	7	56,136,667 (GRCm38)	missense	probably benign	0.00
R1659:Herc2	UTSW	7	56,135,105 (GRCm38)	missense	probably benign	0.00
R1682:Herc2	UTSW	7	56,088,400 (GRCm38)	missense	possibly damaging	0.87
R1697:Herc2	UTSW	7	56,153,905 (GRCm38)	missense	probably benign	0.43
R1748:Herc2	UTSW	7	56,148,823 (GRCm38)	critical splice donor site	probably null
R1802:Herc2	UTSW	7	56,184,332 (GRCm38)	missense	probably damaging	1.00
R1835:Herc2	UTSW	7	56,206,765 (GRCm38)	nonsense	probably null
R1836:Herc2	UTSW	7	56,155,105 (GRCm38)	nonsense	probably null
R1872:Herc2	UTSW	7	56,157,509 (GRCm38)	missense	probably benign	0.18
R1889:Herc2	UTSW	7	56,189,813 (GRCm38)	missense	possibly damaging	0.60
R1906:Herc2	UTSW	7	56,114,864 (GRCm38)	missense	probably benign	0.01
R2004:Herc2	UTSW	7	56,137,859 (GRCm38)	missense	probably damaging	1.00
R2030:Herc2	UTSW	7	56,184,373 (GRCm38)	missense	probably damaging	0.99
R2037:Herc2	UTSW	7	56,205,961 (GRCm38)	missense	probably damaging	1.00
R2059:Herc2	UTSW	7	56,163,897 (GRCm38)	missense	probably damaging	1.00
R2068:Herc2	UTSW	7	56,132,497 (GRCm38)	missense	probably damaging	1.00
R2072:Herc2	UTSW	7	56,226,964 (GRCm38)	missense	probably damaging	1.00
R2085:Herc2	UTSW	7	56,212,965 (GRCm38)	missense	possibly damaging	0.94
R2115:Herc2	UTSW	7	56,185,828 (GRCm38)	splice site	probably benign
R2160:Herc2	UTSW	7	56,212,922 (GRCm38)	missense	probably benign	0.00
R2173:Herc2	UTSW	7	56,185,951 (GRCm38)	missense	probably benign	0.27
R2221:Herc2	UTSW	7	56,169,018 (GRCm38)	critical splice donor site	probably null
R2280:Herc2	UTSW	7	56,137,271 (GRCm38)	missense	possibly damaging	0.79
R3078:Herc2	UTSW	7	56,137,243 (GRCm38)	missense	probably benign
R3104:Herc2	UTSW	7	56,135,355 (GRCm38)	missense	probably benign	0.23
R3177:Herc2	UTSW	7	56,153,428 (GRCm38)	missense	probably benign	0.00
R3277:Herc2	UTSW	7	56,153,428 (GRCm38)	missense	probably benign	0.00
R3766:Herc2	UTSW	7	56,163,824 (GRCm38)	missense	probably damaging	1.00
R3770:Herc2	UTSW	7	56,165,007 (GRCm38)	missense	probably benign
R3807:Herc2	UTSW	7	56,207,809 (GRCm38)	missense	probably damaging	1.00
R3912:Herc2	UTSW	7	56,098,437 (GRCm38)	missense	probably damaging	0.98
R4004:Herc2	UTSW	7	56,106,465 (GRCm38)	missense	possibly damaging	0.53
R4039:Herc2	UTSW	7	56,156,411 (GRCm38)	missense	probably damaging	0.98
R4190:Herc2	UTSW	7	56,122,448 (GRCm38)	missense	probably benign	0.03
R4225:Herc2	UTSW	7	56,164,987 (GRCm38)	missense	probably damaging	1.00
R4334:Herc2	UTSW	7	56,226,654 (GRCm38)	missense	probably damaging	1.00
R4405:Herc2	UTSW	7	56,170,477 (GRCm38)	missense	probably damaging	1.00
R4448:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4450:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4565:Herc2	UTSW	7	56,153,838 (GRCm38)	missense	possibly damaging	0.71
R4667:Herc2	UTSW	7	56,131,253 (GRCm38)	missense	probably damaging	1.00
R4747:Herc2	UTSW	7	56,106,393 (GRCm38)	missense	possibly damaging	0.80
R4762:Herc2	UTSW	7	56,170,640 (GRCm38)	missense	probably benign	0.19
R4829:Herc2	UTSW	7	56,106,492 (GRCm38)	missense	probably benign	0.39
R4832:Herc2	UTSW	7	56,098,417 (GRCm38)	nonsense	probably null
R4895:Herc2	UTSW	7	56,222,986 (GRCm38)	missense	probably damaging	1.00
R4904:Herc2	UTSW	7	56,157,486 (GRCm38)	missense	probably damaging	0.99
R4908:Herc2	UTSW	7	56,177,912 (GRCm38)	missense	probably benign	0.01
R4911:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4921:Herc2	UTSW	7	56,229,690 (GRCm38)	missense	probably benign	0.04
R4939:Herc2	UTSW	7	56,206,736 (GRCm38)	missense	probably damaging	1.00
R5155:Herc2	UTSW	7	56,227,826 (GRCm38)	missense	possibly damaging	0.85
R5184:Herc2	UTSW	7	56,122,351 (GRCm38)	missense	probably damaging	1.00
R5269:Herc2	UTSW	7	56,168,870 (GRCm38)	nonsense	probably null
R5306:Herc2	UTSW	7	56,184,961 (GRCm38)	missense	probably damaging	1.00
R5314:Herc2	UTSW	7	56,219,786 (GRCm38)	missense	probably damaging	0.99
R5369:Herc2	UTSW	7	56,182,700 (GRCm38)	missense	probably damaging	1.00
R5418:Herc2	UTSW	7	56,137,565 (GRCm38)	missense	probably damaging	1.00
R5420:Herc2	UTSW	7	56,203,830 (GRCm38)	missense	probably damaging	0.96
R5463:Herc2	UTSW	7	56,194,262 (GRCm38)	missense	probably damaging	1.00
R5510:Herc2	UTSW	7	56,206,771 (GRCm38)	missense	probably damaging	1.00
R5634:Herc2	UTSW	7	56,206,783 (GRCm38)	missense	probably damaging	1.00
R5638:Herc2	UTSW	7	56,204,416 (GRCm38)	missense	probably benign	0.01
R5690:Herc2	UTSW	7	56,157,705 (GRCm38)	missense	probably benign
R5762:Herc2	UTSW	7	56,197,190 (GRCm38)	missense	possibly damaging	0.68
R5807:Herc2	UTSW	7	56,230,919 (GRCm38)	missense	probably damaging	0.99
R5878:Herc2	UTSW	7	56,124,248 (GRCm38)	missense	probably benign
R6036:Herc2	UTSW	7	56,068,053 (GRCm38)	missense	probably benign	0.01
R6036:Herc2	UTSW	7	56,068,053 (GRCm38)	missense	probably benign	0.01
R6083:Herc2	UTSW	7	56,228,505 (GRCm38)	missense	probably benign	0.00
R6192:Herc2	UTSW	7	56,207,762 (GRCm38)	missense	probably damaging	1.00
R6193:Herc2	UTSW	7	56,156,901 (GRCm38)	missense	probably damaging	0.98
R6261:Herc2	UTSW	7	56,197,072 (GRCm38)	nonsense	probably null
R6267:Herc2	UTSW	7	56,153,166 (GRCm38)	nonsense	probably null
R6267:Herc2	UTSW	7	56,204,718 (GRCm38)	missense	possibly damaging	0.51
R6299:Herc2	UTSW	7	56,135,055 (GRCm38)	missense	possibly damaging	0.47
R6326:Herc2	UTSW	7	56,222,934 (GRCm38)	missense	probably damaging	0.98
R6347:Herc2	UTSW	7	56,194,403 (GRCm38)	critical splice donor site	probably null
R6394:Herc2	UTSW	7	56,215,981 (GRCm38)	missense	probably damaging	1.00
R6500:Herc2	UTSW	7	56,146,645 (GRCm38)	nonsense	probably null
R6526:Herc2	UTSW	7	56,157,330 (GRCm38)	missense	probably damaging	0.99
R6592:Herc2	UTSW	7	56,207,690 (GRCm38)	critical splice acceptor site	probably null
R6619:Herc2	UTSW	7	56,068,092 (GRCm38)	nonsense	probably null
R6719:Herc2	UTSW	7	56,212,826 (GRCm38)	missense	probably damaging	1.00
R6750:Herc2	UTSW	7	56,097,447 (GRCm38)	missense	probably damaging	1.00
R6807:Herc2	UTSW	7	56,164,922 (GRCm38)	missense	probably damaging	1.00
R6811:Herc2	UTSW	7	56,113,433 (GRCm38)	nonsense	probably null
R6837:Herc2	UTSW	7	56,189,841 (GRCm38)	missense	possibly damaging	0.89
R6838:Herc2	UTSW	7	56,108,778 (GRCm38)	missense	probably damaging	1.00
R6902:Herc2	UTSW	7	56,135,486 (GRCm38)	missense	probably benign	0.37
R6983:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6985:Herc2	UTSW	7	56,132,480 (GRCm38)	missense	probably damaging	1.00
R6985:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6986:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6987:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R7113:Herc2	UTSW	7	56,203,849 (GRCm38)	missense	probably damaging	0.99
R7173:Herc2	UTSW	7	56,203,827 (GRCm38)	missense	probably damaging	1.00
R7202:Herc2	UTSW	7	56,131,286 (GRCm38)	missense	probably damaging	0.99
R7205:Herc2	UTSW	7	56,182,640 (GRCm38)	missense	probably damaging	1.00
R7236:Herc2	UTSW	7	56,085,080 (GRCm38)	missense	probably benign	0.29
R7297:Herc2	UTSW	7	56,136,658 (GRCm38)	missense	probably benign	0.00
R7358:Herc2	UTSW	7	56,182,675 (GRCm38)	missense	possibly damaging	0.48
R7438:Herc2	UTSW	7	56,103,718 (GRCm38)	splice site	probably null
R7537:Herc2	UTSW	7	56,219,779 (GRCm38)	nonsense	probably null
R7578:Herc2	UTSW	7	56,134,800 (GRCm38)	missense	probably benign	0.07
R7614:Herc2	UTSW	7	56,153,275 (GRCm38)	nonsense	probably null
R7638:Herc2	UTSW	7	56,157,438 (GRCm38)	missense	probably benign	0.26
R7638:Herc2	UTSW	7	56,220,525 (GRCm38)	missense	probably damaging	1.00
R7646:Herc2	UTSW	7	56,134,613 (GRCm38)	missense	probably benign
R7663:Herc2	UTSW	7	56,136,685 (GRCm38)	missense	probably benign
R7665:Herc2	UTSW	7	56,153,155 (GRCm38)	missense	probably damaging	1.00
R7691:Herc2	UTSW	7	56,191,845 (GRCm38)	missense	probably benign
R7733:Herc2	UTSW	7	56,188,664 (GRCm38)	missense	probably damaging	0.99
R7767:Herc2	UTSW	7	56,228,527 (GRCm38)	missense	probably benign	0.39
R7802:Herc2	UTSW	7	56,164,090 (GRCm38)	missense	probably damaging	1.00
R7847:Herc2	UTSW	7	56,157,560 (GRCm38)	critical splice donor site	probably null
R7956:Herc2	UTSW	7	56,113,400 (GRCm38)	missense	probably damaging	0.97
R7985:Herc2	UTSW	7	56,165,244 (GRCm38)	missense	probably benign
R8003:Herc2	UTSW	7	56,168,904 (GRCm38)	missense	possibly damaging	0.94
R8045:Herc2	UTSW	7	56,184,900 (GRCm38)	missense	probably damaging	1.00
R8085:Herc2	UTSW	7	56,229,679 (GRCm38)	missense	probably benign	0.01
R8134:Herc2	UTSW	7	56,085,136 (GRCm38)	missense	probably benign	0.10
R8259:Herc2	UTSW	7	56,205,890 (GRCm38)	missense	probably damaging	0.99
R8286:Herc2	UTSW	7	56,229,662 (GRCm38)	missense	possibly damaging	0.92
R8304:Herc2	UTSW	7	56,159,438 (GRCm38)	missense	probably damaging	1.00
R8321:Herc2	UTSW	7	56,229,348 (GRCm38)	missense	possibly damaging	0.84
R8332:Herc2	UTSW	7	56,146,595 (GRCm38)	missense	probably damaging	1.00
R8432:Herc2	UTSW	7	56,155,112 (GRCm38)	missense	probably benign	0.14
R8516:Herc2	UTSW	7	56,206,570 (GRCm38)	missense	probably benign	0.05
R8676:Herc2	UTSW	7	56,188,613 (GRCm38)	missense	probably damaging	1.00
R8738:Herc2	UTSW	7	56,148,654 (GRCm38)	missense	possibly damaging	0.78
R8742:Herc2	UTSW	7	56,094,395 (GRCm38)	missense	probably benign	0.12
R8796:Herc2	UTSW	7	56,135,375 (GRCm38)	missense	probably benign	0.01
R8825:Herc2	UTSW	7	56,050,878 (GRCm38)	start codon destroyed	probably null	0.01
R8826:Herc2	UTSW	7	56,106,396 (GRCm38)	missense	probably benign	0.12
R8842:Herc2	UTSW	7	56,088,311 (GRCm38)	missense	probably damaging	0.99
R9103:Herc2	UTSW	7	56,135,055 (GRCm38)	missense	possibly damaging	0.47
R9124:Herc2	UTSW	7	56,184,308 (GRCm38)	missense	probably damaging	1.00
R9134:Herc2	UTSW	7	56,182,429 (GRCm38)	missense	probably damaging	0.99
R9168:Herc2	UTSW	7	56,152,460 (GRCm38)	missense	probably damaging	0.99
R9173:Herc2	UTSW	7	56,206,602 (GRCm38)	missense	probably damaging	0.97
R9238:Herc2	UTSW	7	56,163,760 (GRCm38)	missense	probably damaging	0.98
R9249:Herc2	UTSW	7	56,113,142 (GRCm38)	missense	probably damaging	1.00
R9344:Herc2	UTSW	7	56,122,364 (GRCm38)	missense	probably benign	0.07
R9432:Herc2	UTSW	7	56,131,184 (GRCm38)	missense	probably damaging	1.00
R9472:Herc2	UTSW	7	56,164,095 (GRCm38)	missense	probably damaging	1.00
R9513:Herc2	UTSW	7	56,113,100 (GRCm38)	missense	probably damaging	1.00
R9579:Herc2	UTSW	7	56,108,752 (GRCm38)	missense	probably damaging	0.99
R9596:Herc2	UTSW	7	56,184,847 (GRCm38)	missense
R9664:Herc2	UTSW	7	56,170,590 (GRCm38)	missense	possibly damaging	0.90
R9760:Herc2	UTSW	7	56,163,911 (GRCm38)	critical splice donor site	probably null
R9781:Herc2	UTSW	7	56,100,348 (GRCm38)	missense	possibly damaging	0.53
RF024:Herc2	UTSW	7	56,226,525 (GRCm38)	missense	probably damaging	1.00
X0011:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
X0023:Herc2	UTSW	7	56,090,918 (GRCm38)	missense	possibly damaging	0.73
X0057:Herc2	UTSW	7	56,229,690 (GRCm38)	missense	probably benign	0.04
X0064:Herc2	UTSW	7	56,191,258 (GRCm38)	missense	probably benign
X0064:Herc2	UTSW	7	56,191,211 (GRCm38)	missense	probably benign	0.01
Z1088:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1088:Herc2	UTSW	7	56,087,341 (GRCm38)	missense	probably benign	0.00
Z1088:Herc2	UTSW	7	56,226,589 (GRCm38)	missense	probably damaging	1.00
Z1088:Herc2	UTSW	7	56,215,432 (GRCm38)	missense	probably damaging	1.00
Z1088:Herc2	UTSW	7	56,215,381 (GRCm38)	missense	possibly damaging	0.86
Z1176:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1176:Herc2	UTSW	7	56,097,533 (GRCm38)	missense	possibly damaging	0.48
Z1176:Herc2	UTSW	7	56,132,498 (GRCm38)	missense	probably damaging	1.00
Z1177:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1177:Herc2	UTSW	7	56,121,589 (GRCm38)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- AGACATACTCTTAGTGAGCACTTGAC -3'
(R):5'- AGCTCAAAATCAGATAGCGGAC -3'

Sequencing Primer
(F):5'- AGTGAGCACTTGACCTATCCTG -3'
(R):5'- CAAAATCAGATAGCGGACACAAAATG -3'

Posted On

2018-04-02