Mutagenetix > Incidental Mutation

Incidental Mutation 'R6318:Ceacam14'

510277

Institutional Source

Beutler Lab

Gene Symbol

Ceacam14

Ensembl Gene

ENSMUSG00000023185

Gene Name

carcinoembryonic antigen-related cell adhesion molecule 14

Synonyms

1600025E09Rik, 1600021E03Rik

MMRRC Submission

044473-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6318 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

17812633-17815663 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 17814312 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 109 (I109T)

Ref Sequence

ENSEMBL: ENSMUSP00000023953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023953]

AlphaFold

Q78Y72

Predicted Effect

probably damaging
Transcript: ENSMUST00000023953
AA Change: I109T

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000023953
Gene: ENSMUSG00000023185
AA Change: I109T

Domain	Start	End	E-Value	Type
low complexity region	18	29	N/A	INTRINSIC
IG_like	40	141	6.35e0	SMART
IG_like	158	261	2.73e1	SMART

Meta Mutation Damage Score

0.3519

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 97.3%

Validation Efficiency

99% (74/75)

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700102P08Rik	A	G	9: 108,393,275 (GRCm38)	T13A	possibly damaging	Het
Abcg4	T	C	9: 44,275,348 (GRCm38)	T500A	probably benign	Het
Adcy4	T	A	14: 55,769,224 (GRCm38)	I1051F	probably damaging	Het
Adgrg6	A	T	10: 14,467,497 (GRCm38)	N235K	probably benign	Het
Aldh3a2	A	C	11: 61,262,419 (GRCm38)	Y160*	probably null	Het
Arhgef4	G	A	1: 34,723,477 (GRCm38)	A605T	unknown	Het
Armt1	T	C	10: 4,450,859 (GRCm38)	M202T	probably benign	Het
AU019823	T	C	9: 50,607,461 (GRCm38)	R284G	probably benign	Het
Brd2	C	A	17: 34,112,898 (GRCm38)	V349F	probably damaging	Het
Btnl10	A	T	11: 58,926,865 (GRCm38)		probably benign	Het
Ccdc178	A	G	18: 22,120,534 (GRCm38)	V216A	possibly damaging	Het
Ccdc85c	A	T	12: 108,274,709 (GRCm38)	L142Q	unknown	Het
Cdc27	A	T	11: 104,528,694 (GRCm38)	S172T	probably damaging	Het
Ces5a	C	T	8: 93,534,583 (GRCm38)	G72E	probably damaging	Het
Cfb	A	G	17: 34,861,824 (GRCm38)	Y66H	possibly damaging	Het
Clec14a	G	A	12: 58,268,215 (GRCm38)	P207L	probably damaging	Het
Clec16a	G	A	16: 10,630,788 (GRCm38)	R599H	probably damaging	Het
Csmd1	A	T	8: 15,903,212 (GRCm38)	I3423N	probably damaging	Het
Cyp4a29	C	A	4: 115,250,199 (GRCm38)	N243K	probably benign	Het
Ddx59	T	C	1: 136,416,872 (GRCm38)	F94L	probably damaging	Het
Dusp3	A	C	11: 101,986,871 (GRCm38)	V19G	probably benign	Het
Fat3	T	C	9: 15,916,984 (GRCm38)		probably benign	Het
Fgfr4	T	A	13: 55,166,108 (GRCm38)	V545E	probably damaging	Het
Fxn	G	T	19: 24,280,426 (GRCm38)	A47D	probably damaging	Het
Gdpgp1	A	T	7: 80,239,150 (GRCm38)	I310F	possibly damaging	Het
Gm7210	A	T	7: 11,594,113 (GRCm38)		noncoding transcript	Het
Grm7	G	T	6: 111,358,875 (GRCm38)	C749F	probably damaging	Het
Hps4	T	G	5: 112,346,629 (GRCm38)	V26G	probably damaging	Het
Igf1r	A	G	7: 68,165,233 (GRCm38)	D294G	probably benign	Het
Immp1l	T	C	2: 105,930,827 (GRCm38)	F27S	probably benign	Het
Kcnk3	T	A	5: 30,622,586 (GRCm38)	C327S	probably damaging	Het
Kif13a	T	A	13: 46,815,207 (GRCm38)		probably null	Het
Krtap5-4	T	C	7: 142,304,090 (GRCm38)	S166P	unknown	Het
Lyst	G	A	13: 13,743,311 (GRCm38)	D3319N	possibly damaging	Het
Malrd1	T	G	2: 16,042,267 (GRCm38)	S1735A	unknown	Het
Myh4	A	G	11: 67,243,442 (GRCm38)	T308A	probably benign	Het
Myh8	A	T	11: 67,299,341 (GRCm38)	Q1269L	probably benign	Het
Myo15b	G	A	11: 115,890,831 (GRCm38)	V1367I	probably damaging	Het
Nae1	T	C	8: 104,523,637 (GRCm38)	D208G	probably benign	Het
Nelfe	T	A	17: 34,854,456 (GRCm38)	V296D	probably damaging	Het
Npepps	A	T	11: 97,218,548 (GRCm38)	V734E	probably damaging	Het
Ogdh	A	G	11: 6,349,390 (GRCm38)	N752S	probably damaging	Het
Olfr1094	A	G	2: 86,829,654 (GRCm38)	I301V	possibly damaging	Het
Olfr266	T	C	3: 106,822,187 (GRCm38)	D124G	probably damaging	Het
Olfr401	A	T	11: 74,121,721 (GRCm38)	Q144L	possibly damaging	Het
Olfr906	T	C	9: 38,488,377 (GRCm38)	M116T	probably benign	Het
Otof	C	A	5: 30,414,544 (GRCm38)	G171V	probably damaging	Het
Phtf2	A	T	5: 20,801,941 (GRCm38)	V208D	probably damaging	Het
Pkn1	T	A	8: 83,683,591 (GRCm38)	T340S	probably damaging	Het
Plcd4	C	T	1: 74,563,594 (GRCm38)	L668F	possibly damaging	Het
Plch1	C	T	3: 63,781,390 (GRCm38)	W131*	probably null	Het
Prss50	A	T	9: 110,861,299 (GRCm38)	D170V	probably damaging	Het
Ptprg	T	C	14: 12,237,118 (GRCm38)	V604A	probably damaging	Het
Rab33b	T	C	3: 51,493,405 (GRCm38)	V100A	probably damaging	Het
Rbm26	T	A	14: 105,131,535 (GRCm38)	D736V	probably damaging	Het
Rela	C	A	19: 5,646,964 (GRCm38)	P400T	probably benign	Het
Rpusd4	T	C	9: 35,268,038 (GRCm38)	L50P	probably damaging	Het
Scn10a	A	C	9: 119,627,115 (GRCm38)	Y1214D	probably damaging	Het
Sema3c	A	G	5: 17,672,432 (GRCm38)	E179G	probably damaging	Het
Skint5	T	A	4: 113,517,133 (GRCm38)	D1253V	unknown	Het
Sp4	G	T	12: 118,238,178 (GRCm38)	P771H	probably damaging	Het
Sphkap	T	A	1: 83,278,378 (GRCm38)	Y263F	probably damaging	Het
Ssbp1	G	A	6: 40,476,753 (GRCm38)	V78I	probably benign	Het
St3gal6	A	G	16: 58,486,406 (GRCm38)	I87T	probably benign	Het
Tango6	T	A	8: 106,818,497 (GRCm38)	D997E	probably benign	Het
Tpr	C	T	1: 150,445,888 (GRCm38)	P2265S	possibly damaging	Het
Ttc7b	A	T	12: 100,325,677 (GRCm38)	F212Y	probably damaging	Het
Ubc	A	G	5: 125,388,260 (GRCm38)	M1T	probably null	Het
Vill	G	C	9: 119,063,648 (GRCm38)	Q376H	probably benign	Het
Yes1	T	C	5: 32,651,686 (GRCm38)	I132T	possibly damaging	Het
Ythdc2	A	G	18: 44,860,377 (GRCm38)	T830A	probably benign	Het
Zbtb41	T	A	1: 139,430,306 (GRCm38)	F451I	possibly damaging	Het
Zfp995	C	T	17: 21,880,512 (GRCm38)	C247Y	probably benign	Het

Other mutations in Ceacam14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Ceacam14	APN	7	17,814,137 (GRCm38)	missense	probably damaging	1.00
R0452:Ceacam14	UTSW	7	17,815,323 (GRCm38)	missense	probably benign
R1613:Ceacam14	UTSW	7	17,814,048 (GRCm38)	splice site	probably benign
R1990:Ceacam14	UTSW	7	17,815,365 (GRCm38)	nonsense	probably null
R2153:Ceacam14	UTSW	7	17,814,228 (GRCm38)	missense	probably benign	0.00
R3887:Ceacam14	UTSW	7	17,814,138 (GRCm38)	missense	probably damaging	1.00
R4724:Ceacam14	UTSW	7	17,814,050 (GRCm38)	critical splice acceptor site	probably null
R5323:Ceacam14	UTSW	7	17,815,477 (GRCm38)	makesense	probably null
R5454:Ceacam14	UTSW	7	17,814,185 (GRCm38)	missense	probably damaging	1.00
R5696:Ceacam14	UTSW	7	17,814,342 (GRCm38)	missense	probably damaging	0.97
R6763:Ceacam14	UTSW	7	17,815,343 (GRCm38)	missense	probably benign	0.04
R7607:Ceacam14	UTSW	7	17,814,321 (GRCm38)	missense	possibly damaging	0.95
R7789:Ceacam14	UTSW	7	17,814,171 (GRCm38)	missense	probably damaging	0.99
R8946:Ceacam14	UTSW	7	17,814,074 (GRCm38)	missense	probably benign	0.01
R9781:Ceacam14	UTSW	7	17,815,157 (GRCm38)	missense	possibly damaging	0.60

Predicted Primers

PCR Primer
(F):5'- TCAAATCAGTGCCTCCCATTG -3'
(R):5'- GGAGATCCAGTTTAGGCCTG -3'

Sequencing Primer
(F):5'- CCTCCCATTGCTGTTGAAGGG -3'
(R):5'- CAGTTTAGGCCTGACATTTTTCAG -3'

Posted On

2018-04-02