Mutagenetix > Incidental Mutation

Incidental Mutation 'R6315:Helz2'

510573

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

MMRRC Submission

044472-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6315 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

180869408-180883820 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 180874995 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 1833 (L1833P)

Ref Sequence

ENSEMBL: ENSMUSP00000112917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094203
AA Change: L1833P

PolyPhen 2 Score 0.955 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: L1833P

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000108831
AA Change: L1833P

PolyPhen 2 Score 0.970 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: L1833P

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000121484
AA Change: L1833P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: L1833P

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149417

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155049

Meta Mutation Damage Score

0.8116

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

97% (64/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	T	C	7: 119,945,315 (GRCm39)	Y464H	probably damaging	Het
Ahnak	T	C	19: 8,983,990 (GRCm39)	M1758T	probably damaging	Het
Ak9	A	T	10: 41,282,837 (GRCm39)	D1201V	possibly damaging	Het
Amdhd2	A	C	17: 24,377,330 (GRCm39)	H203Q	probably benign	Het
Ano3	A	G	2: 110,527,384 (GRCm39)	M608T	probably damaging	Het
Arhgef4	G	A	1: 34,762,558 (GRCm39)	A605T	unknown	Het
Atp1a2	C	G	1: 172,116,903 (GRCm39)	R238P	probably damaging	Het
Atp8b1	C	A	18: 64,664,550 (GRCm39)	R1206L	probably damaging	Het
B4galt5	A	T	2: 167,147,729 (GRCm39)	N215K	probably damaging	Het
Bpifa1	A	G	2: 153,987,996 (GRCm39)	K180E	possibly damaging	Het
Cadm1	C	T	9: 47,721,417 (GRCm39)	T269M	probably damaging	Het
Cadm3	T	C	1: 173,171,919 (GRCm39)	D252G	probably benign	Het
Ccdc18	A	G	5: 108,309,448 (GRCm39)	R348G	probably benign	Het
Cers4	G	A	8: 4,566,980 (GRCm39)	C94Y	probably benign	Het
Cfap58	G	A	19: 47,929,716 (GRCm39)	R59H	probably benign	Het
Col19a1	C	T	1: 24,565,533 (GRCm39)	G266D	unknown	Het
Col6a5	G	T	9: 105,759,169 (GRCm39)	F2012L	probably damaging	Het
Dnah6	T	C	6: 73,168,588 (GRCm39)	Q445R	probably benign	Het
Dsg3	A	G	18: 20,657,643 (GRCm39)	S238G	probably benign	Het
E130308A19Rik	G	A	4: 59,691,132 (GRCm39)	S322N	probably benign	Het
Eif5b	A	G	1: 38,057,114 (GRCm39)	D57G	unknown	Het
Fbn2	T	C	18: 58,188,025 (GRCm39)		probably null	Het
Fbxo38	A	T	18: 62,669,218 (GRCm39)	C77*	probably null	Het
Fgb	T	A	3: 82,952,362 (GRCm39)	Q169L	probably benign	Het
Fut9	A	T	4: 25,619,774 (GRCm39)	Y347N	probably damaging	Het
Gabrg2	T	C	11: 41,862,688 (GRCm39)	K132R	probably damaging	Het
Gpn3	C	T	5: 122,510,638 (GRCm39)		probably benign	Het
Hk3	C	T	13: 55,158,970 (GRCm39)	D484N	probably benign	Het
Icam4	T	C	9: 20,941,248 (GRCm39)	V129A	probably damaging	Het
Impdh2	T	C	9: 108,440,638 (GRCm39)	S280P	possibly damaging	Het
Impg1	C	A	9: 80,301,356 (GRCm39)	G267V	probably benign	Het
Iqgap1	C	T	7: 80,449,638 (GRCm39)	V39M	possibly damaging	Het
Lyst	A	G	13: 13,818,089 (GRCm39)	N1253S	probably benign	Het
Mgat4b	T	A	11: 50,122,591 (GRCm39)	V195E	probably damaging	Het
Mrgprb8	C	T	7: 48,038,983 (GRCm39)	T218I	probably damaging	Het
Myrfl	T	G	10: 116,658,724 (GRCm39)	E411A	possibly damaging	Het
Myt1l	T	C	12: 29,877,797 (GRCm39)	S483P	unknown	Het
Neil2	A	G	14: 63,420,920 (GRCm39)	V80A	possibly damaging	Het
Nfe2l2	G	T	2: 75,507,163 (GRCm39)	D312E	probably damaging	Het
Nin	A	T	12: 70,092,389 (GRCm39)	L640Q	probably damaging	Het
Nup205	T	A	6: 35,213,804 (GRCm39)	I1732N	probably damaging	Het
Nup42	T	C	5: 24,372,502 (GRCm39)	S97P	probably damaging	Het
Or5b114-ps1	T	C	19: 13,352,769 (GRCm39)	C148R	unknown	Het
Or5k16	A	C	16: 58,736,609 (GRCm39)	Y132D	probably damaging	Het
Plcb1	A	T	2: 135,188,261 (GRCm39)	T785S	probably benign	Het
Plch1	C	T	3: 63,688,811 (GRCm39)	W131*	probably null	Het
Ppp1r1b	A	G	11: 98,246,216 (GRCm39)	E66G	probably damaging	Het
Ros1	A	T	10: 51,994,306 (GRCm39)	W1325R	probably benign	Het
Sec14l5	A	C	16: 4,998,141 (GRCm39)	E500A	possibly damaging	Het
Sirpb1c	A	G	3: 15,886,470 (GRCm39)	V302A	possibly damaging	Het
Spata19	T	C	9: 27,312,133 (GRCm39)	Y107H	possibly damaging	Het
Sv2a	G	A	3: 96,095,502 (GRCm39)	M298I	probably benign	Het
Tdrd6	A	T	17: 43,937,229 (GRCm39)	M1273K	probably benign	Het
Tectb	A	G	19: 55,179,904 (GRCm39)	H250R	possibly damaging	Het
Trip11	T	G	12: 101,851,837 (GRCm39)	E742D	possibly damaging	Het
Tslp	A	G	18: 32,950,143 (GRCm39)	T71A	probably benign	Het
Usp20	C	T	2: 30,907,770 (GRCm39)	R711C	possibly damaging	Het
Vmn1r181	G	A	7: 23,684,183 (GRCm39)	R216Q	probably benign	Het
Vmn2r8	A	T	5: 108,949,757 (GRCm39)	F363L	probably benign	Het
Vsnl1	T	A	12: 11,382,156 (GRCm39)	N75I	probably damaging	Het
Wdr55	A	T	18: 36,895,122 (GRCm39)	D96V	probably damaging	Het
Wls	T	A	3: 159,640,007 (GRCm39)		probably null	Het
Zc3h13	A	G	14: 75,546,355 (GRCm39)	D152G	probably damaging	Het
Zc3h18	A	G	8: 123,110,604 (GRCm39)	E151G	probably benign	Het
Zfat	A	G	15: 67,956,311 (GRCm39)	S1174P	probably damaging	Het
Zfyve9	T	A	4: 108,531,685 (GRCm39)	T416S	probably damaging	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	180,871,495 (GRCm39)	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	180,874,799 (GRCm39)	nonsense	probably null
IGL00704:Helz2	APN	2	180,876,178 (GRCm39)	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	180,874,038 (GRCm39)	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	180,875,770 (GRCm39)	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	180,874,674 (GRCm39)	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	180,880,274 (GRCm39)	missense	probably benign	0.29
IGL02116:Helz2	APN	2	180,873,978 (GRCm39)	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	180,873,483 (GRCm39)	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	180,872,704 (GRCm39)	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	180,872,815 (GRCm39)	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	180,876,819 (GRCm39)	missense	probably benign	0.14
IGL02869:Helz2	APN	2	180,872,939 (GRCm39)	intron	probably benign
IGL03003:Helz2	APN	2	180,882,046 (GRCm39)	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	180,871,015 (GRCm39)	critical splice donor site	probably null
IGL03310:Helz2	APN	2	180,873,597 (GRCm39)	missense	probably benign	0.00
Colby	UTSW	2	180,874,995 (GRCm39)	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	180,876,627 (GRCm39)	missense	probably benign	0.03
R0013:Helz2	UTSW	2	180,882,752 (GRCm39)	missense	probably benign
R0013:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	180,882,304 (GRCm39)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	180,882,304 (GRCm39)	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	180,870,614 (GRCm39)	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	180,870,614 (GRCm39)	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	180,878,200 (GRCm39)	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	180,878,200 (GRCm39)	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	180,879,595 (GRCm39)	missense	probably benign	0.30
R0117:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	180,874,062 (GRCm39)	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	180,872,223 (GRCm39)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	180,872,223 (GRCm39)	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	180,872,386 (GRCm39)	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	180,874,002 (GRCm39)	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	180,871,449 (GRCm39)	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	180,869,563 (GRCm39)	missense	probably benign	0.00
R0541:Helz2	UTSW	2	180,876,618 (GRCm39)	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	180,873,882 (GRCm39)	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	180,873,909 (GRCm39)	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	180,882,674 (GRCm39)	missense	probably benign
R0826:Helz2	UTSW	2	180,882,646 (GRCm39)	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	180,872,570 (GRCm39)	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	180,877,928 (GRCm39)	missense	probably benign
R1170:Helz2	UTSW	2	180,871,608 (GRCm39)	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	180,872,921 (GRCm39)	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	180,879,389 (GRCm39)	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	180,874,774 (GRCm39)	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	180,877,317 (GRCm39)	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	180,881,447 (GRCm39)	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	180,881,447 (GRCm39)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	180,874,597 (GRCm39)	missense	probably benign	0.00
R1564:Helz2	UTSW	2	180,875,021 (GRCm39)	missense	probably benign	0.01
R1584:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	180,875,940 (GRCm39)	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	180,878,056 (GRCm39)	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	180,880,252 (GRCm39)	missense	possibly damaging	0.84
R1779:Helz2	UTSW	2	180,876,780 (GRCm39)	missense	probably benign
R1837:Helz2	UTSW	2	180,871,082 (GRCm39)	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	180,873,878 (GRCm39)	missense	probably benign	0.02
R1894:Helz2	UTSW	2	180,876,082 (GRCm39)	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	180,875,543 (GRCm39)	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	180,873,122 (GRCm39)	missense	probably benign	0.45
R2034:Helz2	UTSW	2	180,874,371 (GRCm39)	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	180,879,272 (GRCm39)	missense	probably benign	0.03
R2061:Helz2	UTSW	2	180,882,337 (GRCm39)	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	180,876,895 (GRCm39)	missense	probably benign	0.07
R2142:Helz2	UTSW	2	180,873,173 (GRCm39)	missense	probably benign
R2180:Helz2	UTSW	2	180,875,525 (GRCm39)	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	180,870,841 (GRCm39)	nonsense	probably null
R2248:Helz2	UTSW	2	180,875,226 (GRCm39)	missense	probably benign	0.33
R2495:Helz2	UTSW	2	180,874,705 (GRCm39)	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	180,882,535 (GRCm39)	missense	probably benign
R3617:Helz2	UTSW	2	180,874,854 (GRCm39)	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	180,882,182 (GRCm39)	nonsense	probably null
R3803:Helz2	UTSW	2	180,881,789 (GRCm39)	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	180,871,503 (GRCm39)	missense	probably benign	0.00
R4052:Helz2	UTSW	2	180,882,268 (GRCm39)	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	180,871,695 (GRCm39)	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	180,870,626 (GRCm39)	missense	probably benign
R4624:Helz2	UTSW	2	180,881,101 (GRCm39)	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	180,880,210 (GRCm39)	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	180,879,210 (GRCm39)	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	180,871,913 (GRCm39)	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	180,877,940 (GRCm39)	missense	probably benign	0.01
R4915:Helz2	UTSW	2	180,874,231 (GRCm39)	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	180,882,709 (GRCm39)	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	180,882,362 (GRCm39)	missense	probably benign
R5089:Helz2	UTSW	2	180,876,942 (GRCm39)	missense	probably benign	0.14
R5190:Helz2	UTSW	2	180,872,550 (GRCm39)	critical splice donor site	probably null
R5309:Helz2	UTSW	2	180,876,639 (GRCm39)	missense	probably benign	0.08
R5358:Helz2	UTSW	2	180,877,321 (GRCm39)	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	180,876,862 (GRCm39)	missense	probably benign
R5559:Helz2	UTSW	2	180,871,919 (GRCm39)	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	180,882,051 (GRCm39)	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	180,879,082 (GRCm39)	intron	probably benign
R5805:Helz2	UTSW	2	180,882,301 (GRCm39)	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	180,878,189 (GRCm39)	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	180,874,449 (GRCm39)	missense	probably benign	0.02
R5873:Helz2	UTSW	2	180,875,821 (GRCm39)	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	180,872,177 (GRCm39)	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	180,872,560 (GRCm39)	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	180,872,843 (GRCm39)	missense	probably benign	0.08
R6045:Helz2	UTSW	2	180,882,106 (GRCm39)	missense	probably benign	0.03
R6077:Helz2	UTSW	2	180,874,831 (GRCm39)	missense	probably benign	0.41
R6218:Helz2	UTSW	2	180,874,087 (GRCm39)	missense	probably benign	0.03
R6218:Helz2	UTSW	2	180,877,738 (GRCm39)	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	180,876,862 (GRCm39)	missense	probably benign
R6581:Helz2	UTSW	2	180,871,172 (GRCm39)	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	180,881,350 (GRCm39)	nonsense	probably null
R6964:Helz2	UTSW	2	180,872,221 (GRCm39)	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	180,882,307 (GRCm39)	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	180,873,078 (GRCm39)	missense	probably benign	0.00
R7372:Helz2	UTSW	2	180,880,216 (GRCm39)	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	180,877,393 (GRCm39)	splice site	probably null
R7512:Helz2	UTSW	2	180,872,647 (GRCm39)	missense	probably benign	0.00
R7583:Helz2	UTSW	2	180,879,365 (GRCm39)	missense	probably benign	0.06
R7724:Helz2	UTSW	2	180,873,789 (GRCm39)	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	180,872,148 (GRCm39)	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	180,876,324 (GRCm39)	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	180,875,784 (GRCm39)	missense	probably benign
R7799:Helz2	UTSW	2	180,879,782 (GRCm39)	missense	probably benign	0.15
R7841:Helz2	UTSW	2	180,874,695 (GRCm39)	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	180,879,543 (GRCm39)	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	180,881,998 (GRCm39)	missense	probably benign	0.34
R8030:Helz2	UTSW	2	180,879,689 (GRCm39)	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	180,880,055 (GRCm39)	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	180,871,124 (GRCm39)	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	180,879,895 (GRCm39)	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	180,871,950 (GRCm39)	missense	probably benign	0.03
R8486:Helz2	UTSW	2	180,871,124 (GRCm39)	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	180,871,350 (GRCm39)	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	180,874,560 (GRCm39)	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	180,874,920 (GRCm39)	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	180,880,173 (GRCm39)	missense
R8953:Helz2	UTSW	2	180,874,884 (GRCm39)	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	180,871,407 (GRCm39)	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	180,879,581 (GRCm39)	missense	probably benign	0.19
R8976:Helz2	UTSW	2	180,876,486 (GRCm39)	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	180,870,792 (GRCm39)	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	180,874,261 (GRCm39)	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	180,881,968 (GRCm39)	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	180,881,433 (GRCm39)	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	180,881,848 (GRCm39)	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	180,871,883 (GRCm39)	missense	probably benign
R9186:Helz2	UTSW	2	180,876,457 (GRCm39)	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	180,882,741 (GRCm39)	missense	probably benign
R9407:Helz2	UTSW	2	180,881,975 (GRCm39)	missense	probably benign	0.01
R9465:Helz2	UTSW	2	180,874,710 (GRCm39)	missense	probably benign	0.01
R9502:Helz2	UTSW	2	180,878,245 (GRCm39)	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	180,882,014 (GRCm39)	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	180,882,470 (GRCm39)	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	180,882,025 (GRCm39)	missense	probably benign	0.00
R9800:Helz2	UTSW	2	180,882,616 (GRCm39)	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	180,873,534 (GRCm39)	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	180,879,357 (GRCm39)	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	180,877,754 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTTGCCCATGTGTACCAATC -3'
(R):5'- GCTGAATTCCTTGTGAGCAAC -3'

Sequencing Primer
(F):5'- ATGTGTACCAATCCACCTGTAGCG -3'
(R):5'- GCAACAAGCACACACAGACACTC -3'

Posted On

2018-04-02