Mutagenetix > Incidental Mutation

Incidental Mutation 'R6315:Vmn2r8'

510584

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r8

Ensembl Gene

ENSMUSG00000090961

Gene Name

vomeronasal 2, receptor 8

Synonyms

EG627479

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R6315 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108797193-108808754 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 108801891 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 363 (F363L)

Ref Sequence

ENSEMBL: ENSMUSP00000126953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172140]

AlphaFold

L7N472

Predicted Effect

probably benign
Transcript: ENSMUST00000172140
AA Change: F363L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000126953
Gene: ENSMUSG00000090961
AA Change: F363L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	419	1.1e-28	PFAM
Pfam:NCD3G	507	561	8.2e-18	PFAM
Pfam:7tm_3	594	829	1.1e-54	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

97% (64/66)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	T	C	7: 120,346,092	Y464H	probably damaging	Het
Ahnak	T	C	19: 9,006,626	M1758T	probably damaging	Het
Ak9	A	T	10: 41,406,841	D1201V	possibly damaging	Het
Amdhd2	A	C	17: 24,158,356	H203Q	probably benign	Het
Ano3	A	G	2: 110,697,039	M608T	probably damaging	Het
Arhgef4	G	A	1: 34,723,477	A605T	unknown	Het
Atp1a2	C	G	1: 172,289,336	R238P	probably damaging	Het
Atp8b1	C	A	18: 64,531,479	R1206L	probably damaging	Het
B4galt5	A	T	2: 167,305,809	N215K	probably damaging	Het
Bpifa1	A	G	2: 154,146,076	K180E	possibly damaging	Het
Cadm1	C	T	9: 47,810,119	T269M	probably damaging	Het
Cadm3	T	C	1: 173,344,352	D252G	probably benign	Het
Ccdc18	A	G	5: 108,161,582	R348G	probably benign	Het
Cers4	G	A	8: 4,516,980	C94Y	probably benign	Het
Cfap58	G	A	19: 47,941,277	R59H	probably benign	Het
Col19a1	C	T	1: 24,526,452	G266D	unknown	Het
Col6a5	G	T	9: 105,881,970	F2012L	probably damaging	Het
Dnah6	T	C	6: 73,191,605	Q445R	probably benign	Het
Dsg3	A	G	18: 20,524,586	S238G	probably benign	Het
E130308A19Rik	G	A	4: 59,691,132	S322N	probably benign	Het
Eif5b	A	G	1: 38,018,033	D57G	unknown	Het
Fbn2	T	C	18: 58,054,953		probably null	Het
Fbxo38	A	T	18: 62,536,147	C77*	probably null	Het
Fgb	T	A	3: 83,045,055	Q169L	probably benign	Het
Fut9	A	T	4: 25,619,774	Y347N	probably damaging	Het
Gabrg2	T	C	11: 41,971,861	K132R	probably damaging	Het
Gpn3	C	T	5: 122,372,575		probably benign	Het
Helz2	A	G	2: 181,233,202	L1833P	probably damaging	Het
Hk3	C	T	13: 55,011,157	D484N	probably benign	Het
Icam4	T	C	9: 21,029,952	V129A	probably damaging	Het
Impdh2	T	C	9: 108,563,439	S280P	possibly damaging	Het
Impg1	C	A	9: 80,394,074	G267V	probably benign	Het
Iqgap1	C	T	7: 80,799,890	V39M	possibly damaging	Het
Lyst	A	G	13: 13,643,504	N1253S	probably benign	Het
Mgat4b	T	A	11: 50,231,764	V195E	probably damaging	Het
Mrgprb8	C	T	7: 48,389,235	T218I	probably damaging	Het
Myrfl	T	G	10: 116,822,819	E411A	possibly damaging	Het
Myt1l	T	C	12: 29,827,798	S483P	unknown	Het
Neil2	A	G	14: 63,183,471	V80A	possibly damaging	Het
Nfe2l2	G	T	2: 75,676,819	D312E	probably damaging	Het
Nin	A	T	12: 70,045,615	L640Q	probably damaging	Het
Nup205	T	A	6: 35,236,869	I1732N	probably damaging	Het
Nupl2	T	C	5: 24,167,504	S97P	probably damaging	Het
Olfr1468-ps1	T	C	19: 13,375,405	C148R	unknown	Het
Olfr180	A	C	16: 58,916,246	Y132D	probably damaging	Het
Plcb1	A	T	2: 135,346,341	T785S	probably benign	Het
Plch1	C	T	3: 63,781,390	W131*	probably null	Het
Ppp1r1b	A	G	11: 98,355,390	E66G	probably damaging	Het
Ros1	A	T	10: 52,118,210	W1325R	probably benign	Het
Sec14l5	A	C	16: 5,180,277	E500A	possibly damaging	Het
Sirpb1c	A	G	3: 15,832,306	V302A	possibly damaging	Het
Spata19	T	C	9: 27,400,837	Y107H	possibly damaging	Het
Sv2a	G	A	3: 96,188,186	M298I	probably benign	Het
Tdrd6	A	T	17: 43,626,338	M1273K	probably benign	Het
Tectb	A	G	19: 55,191,472	H250R	possibly damaging	Het
Trip11	T	G	12: 101,885,578	E742D	possibly damaging	Het
Tslp	A	G	18: 32,817,090	T71A	probably benign	Het
Usp20	C	T	2: 31,017,758	R711C	possibly damaging	Het
Vmn1r181	G	A	7: 23,984,758	R216Q	probably benign	Het
Vsnl1	T	A	12: 11,332,155	N75I	probably damaging	Het
Wdr55	A	T	18: 36,762,069	D96V	probably damaging	Het
Wls	T	A	3: 159,934,371		probably null	Het
Zc3h13	A	G	14: 75,308,915	D152G	probably damaging	Het
Zc3h18	A	G	8: 122,383,865	E151G	probably benign	Het
Zfat	A	G	15: 68,084,462	S1174P	probably damaging	Het
Zfyve9	T	A	4: 108,674,488	T416S	probably damaging	Het

Other mutations in Vmn2r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02957:Vmn2r8	APN	5	108802225	missense	probably benign	0.01
R0324:Vmn2r8	UTSW	5	108797941	splice site	probably null
R0335:Vmn2r8	UTSW	5	108797451	splice site	probably null
R0394:Vmn2r8	UTSW	5	108802072	missense	probably benign	0.12
R0501:Vmn2r8	UTSW	5	108803183	missense	probably benign	0.03
R0615:Vmn2r8	UTSW	5	108799329	missense	probably damaging	1.00
R0678:Vmn2r8	UTSW	5	108800546	missense	probably benign	0.00
R1167:Vmn2r8	UTSW	5	108803176	missense	probably benign	0.01
R1187:Vmn2r8	UTSW	5	108803219	nonsense	probably null
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1451:Vmn2r8	UTSW	5	108798067	missense	probably damaging	1.00
R1535:Vmn2r8	UTSW	5	108802174	missense	probably damaging	1.00
R1795:Vmn2r8	UTSW	5	108803106	missense	probably benign
R1874:Vmn2r8	UTSW	5	108802418	missense	possibly damaging	0.74
R1908:Vmn2r8	UTSW	5	108797570	missense	probably benign	0.03
R1925:Vmn2r8	UTSW	5	108802153	missense	probably damaging	0.97
R1960:Vmn2r8	UTSW	5	108799286	missense	probably damaging	0.99
R1961:Vmn2r8	UTSW	5	108798095	missense	probably benign	0.45
R1967:Vmn2r8	UTSW	5	108802383	missense	probably benign	0.01
R2095:Vmn2r8	UTSW	5	108808621	missense	possibly damaging	0.94
R2159:Vmn2r8	UTSW	5	108802303	missense	probably benign	0.22
R4240:Vmn2r8	UTSW	5	108797503	missense	probably damaging	0.99
R4581:Vmn2r8	UTSW	5	108801704	missense	probably benign	0.03
R4744:Vmn2r8	UTSW	5	108808581	missense	probably benign	0.00
R4755:Vmn2r8	UTSW	5	108801700	missense	probably benign	0.03
R4917:Vmn2r8	UTSW	5	108797398	missense	probably damaging	1.00
R4957:Vmn2r8	UTSW	5	108799263	missense	probably benign	0.16
R5141:Vmn2r8	UTSW	5	108808706	missense	probably damaging	0.96
R5481:Vmn2r8	UTSW	5	108801770	missense	probably benign	0.09
R5571:Vmn2r8	UTSW	5	108802240	missense	probably damaging	1.00
R5624:Vmn2r8	UTSW	5	108802459	missense	probably damaging	0.99
R6003:Vmn2r8	UTSW	5	108797382	missense	probably damaging	1.00
R6243:Vmn2r8	UTSW	5	108799345	missense	probably benign	0.01
R6265:Vmn2r8	UTSW	5	108808597	missense	probably benign
R6413:Vmn2r8	UTSW	5	108801723	missense	probably benign	0.09
R7120:Vmn2r8	UTSW	5	108808638	missense	possibly damaging	0.56
R7406:Vmn2r8	UTSW	5	108800576	missense	probably benign	0.00
R7409:Vmn2r8	UTSW	5	108808583	nonsense	probably null
R7489:Vmn2r8	UTSW	5	108797656	missense	possibly damaging	0.95
R7532:Vmn2r8	UTSW	5	108802240	missense	probably benign	0.22
R7534:Vmn2r8	UTSW	5	108802174	missense	possibly damaging	0.94
R7739:Vmn2r8	UTSW	5	108802177	missense	probably damaging	1.00
R8099:Vmn2r8	UTSW	5	108801834	missense	probably benign
R8245:Vmn2r8	UTSW	5	108798070	missense	probably damaging	1.00
R8711:Vmn2r8	UTSW	5	108798096	missense	possibly damaging	0.89
R8781:Vmn2r8	UTSW	5	108797731	missense	possibly damaging	0.95
R8874:Vmn2r8	UTSW	5	108808751	missense	probably damaging	1.00
R8927:Vmn2r8	UTSW	5	108802265	missense
R8928:Vmn2r8	UTSW	5	108802265	missense
R9288:Vmn2r8	UTSW	5	108802319	missense	probably benign	0.39
R9596:Vmn2r8	UTSW	5	108799330	missense	possibly damaging	0.94
R9652:Vmn2r8	UTSW	5	108803241	missense	probably benign	0.18
Z1088:Vmn2r8	UTSW	5	108801998	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CAGTCAGTGAATATTCCTTTGGG -3'
(R):5'- CATCACAATGGGACGTTATCAC -3'

Sequencing Primer
(F):5'- CAGTGAATATTCCTTTGGGTACTTC -3'
(R):5'- ATGGGACGTTATCACAAATAAAAATG -3'

Posted On

2018-04-02